LOCUS       NM_001410606            1919 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus asparagine synthetase (Asns), transcript variant 3,
            mRNA.
ACCESSION   NM_001410606 XM_006505092
VERSION     NM_001410606.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   3  (bases 1 to 1919)
  AUTHORS   Zhu,Y., Ackers-Johnson,M., Shanmugam,M.K., Pakkiri,L.S., Drum,C.L.,
            Chen,Y., Kim,J., Paltzer,W.G., Mahmoud,A.I., Tan,W.L.W.,
            Lee,M.C.J., Jiang,J., Luu,D.A.T., Ng,S.L., Li,P.Y.Q., Wang,A.,
            Qi,R., Ong,G.J.X., Ng,T.Y., Haigh,J.J., Tiang,Z., Richards,A.M. and
            Foo,R.S.Y.
  TITLE     Asparagine Synthetase Marks a Distinct Dependency Threshold for
            Cardiomyocyte Dedifferentiation
  JOURNAL   Circulation 149 (23), 1833-1851 (2024)
   PUBMED   38586957
  REMARK    GeneRIF: Asparagine Synthetase Marks a Distinct Dependency
            Threshold for Cardiomyocyte Dedifferentiation.
REFERENCE   4  (bases 1 to 1919)
  AUTHORS   Clerici,S., Podrini,C., Stefanoni,D., Distefano,G., Cassina,L.,
            Steidl,M.E., Tronci,L., Canu,T., Chiaravalli,M., Spies,D.,
            Bell,T.A. 3rd, Costa,A.S., Esposito,A., D'Alessandro,A., Frezza,C.,
            Bachi,A. and Boletta,A.
  TITLE     Inhibition of asparagine synthetase effectively retards polycystic
            kidney disease progression
  JOURNAL   EMBO Mol Med 16 (6), 1379-1403 (2024)
   PUBMED   38684863
  REMARK    GeneRIF: Inhibition of asparagine synthetase effectively retards
            polycystic kidney disease progression.
REFERENCE   5  (bases 1 to 1919)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 1919)
  AUTHORS   Ruzzo,E.K., Capo-Chichi,J.M., Ben-Zeev,B., Chitayat,D., Mao,H.,
            Pappas,A.L., Hitomi,Y., Lu,Y.F., Yao,X., Hamdan,F.F., Pelak,K.,
            Reznik-Wolf,H., Bar-Joseph,I., Oz-Levi,D., Lev,D., Lerman-Sagie,T.,
            Leshinsky-Silver,E., Anikster,Y., Ben-Asher,E., Olender,T.,
            Colleaux,L., Decarie,J.C., Blaser,S., Banwell,B., Joshi,R.B.,
            He,X.P., Patry,L., Silver,R.J., Dobrzeniecka,S., Islam,M.S.,
            Hasnat,A., Samuels,M.E., Aryal,D.K., Rodriguiz,R.M., Jiang,Y.H.,
            Wetsel,W.C., McNamara,J.O., Rouleau,G.A., Silver,D.L., Lancet,D.,
            Pras,E., Mitchell,G.A., Michaud,J.L. and Goldstein,D.B.
  TITLE     Deficiency of asparagine synthetase causes congenital microcephaly
            and a progressive form of encephalopathy
  JOURNAL   Neuron 80 (2), 429-441 (2013)
   PUBMED   24139043
REFERENCE   7  (bases 1 to 1919)
  AUTHORS   Iida,S., Kamiya,H., Nakaya,A., Hayashi,Y., Sawada,A., Kaji,N.,
            Baba,Y. and Harashima,H.
  TITLE     Reduced plasma glucose by asparagine synthetase knockdown in the
            mouse liver
  JOURNAL   Biol Pharm Bull 36 (12), 2009-2011 (2013)
   PUBMED   24292060
  REMARK    GeneRIF: Asns is involved in the regulation of plasma glucose
            levels.
REFERENCE   8  (bases 1 to 1919)
  AUTHORS   Seo,J., Fortuno,E.S. 3rd, Suh,J.M., Stenesen,D., Tang,W.,
            Parks,E.J., Adams,C.M., Townes,T. and Graff,J.M.
  TITLE     Atf4 regulates obesity, glucose homeostasis, and energy expenditure
  JOURNAL   Diabetes 58 (11), 2565-2573 (2009)
   PUBMED   19690063
REFERENCE   9  (bases 1 to 1919)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   10 (bases 1 to 1919)
  AUTHORS   Van Dyck,F., Braem,C.V., Chen,Z., Declercq,J., Deckers,R.,
            Kim,B.M., Ito,S., Wu,M.K., Cohen,D.E., Dewerchin,M., Derua,R.,
            Waelkens,E., Fiette,L., Roebroek,A., Schuit,F., Van de Ven,W.J. and
            Shivdasani,R.A.
  TITLE     Loss of the PlagL2 transcription factor affects lacteal uptake of
            chylomicrons
  JOURNAL   Cell Metab 6 (5), 406-413 (2007)
   PUBMED   17983586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129291.4.
            
            On Aug 13, 2022 this sequence version replaced XM_006505092.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.130067.1,
                                           SRR5189679.113074.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC129291.4         86837-86900
            65-336              AC129291.4         90224-90495
            337-574             AC129291.4         94341-94578
            575-760             AC129291.4         97291-97476
            761-862             AC129291.4         97712-97813
            863-990             AC129291.4         98370-98497
            991-1117            AC129291.4         99536-99662
            1118-1224           AC129291.4         101701-101807
            1225-1325           AC129291.4         102416-102516
            1326-1407           AC129291.4         103431-103512
            1408-1563           AC129291.4         103599-103754
            1564-1919           AC129291.4         104223-104578
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 3.31 cM"
     gene            1..1919
                     /gene="Asns"
                     /note="asparagine synthetase"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
     exon            1..64
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            65..336
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1773
                     /gene="Asns"
                     /EC_number="6.3.5.4"
                     /note="isoform a is encoded by transcript variant 3;
                     glutamine-dependent asparagine synthetase"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]
                     isoform a"
                     /protein_id="NP_001397535.1"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVRKYPYLWLCYNGEIYNHKALQQRFEFEYQTNVDGEIIL
                     HLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
                     AKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDS
                     VEKLFPGFDLETVKNNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKE
                     AQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLKELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPDMRIPKNGIEKHLLRE
                     TFEDCNLLPKEILWRPKEAFSDGITSVKNSWFKILQDYVEHQVDDEMMSAASQKFPFN
                     TPKTKEGYFYRQIFERHYPGRADWLTHYWMPKWINATDPSARTLTHYKSAAKA"
     exon            337..574
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            575..760
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            761..862
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            863..990
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1117
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1224
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1325
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1407
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1563
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1919
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1898..1903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asns"
                     /note="hexamer: AATAAA"
     polyA_site      1919
                     /gene="Asns"
                     /note="major polyA site"
ORIGIN      
        1 aggaaagctt tcatgtgagc gaagtgcagc gcggaggcag ggccgctcca gacagaaggc
       61 gaagagtgcc tgcagtccgc ctgtagcatg tgtggcatct gggccctctt cggcagcgat
      121 gactgccttt ccgtgcagtg tctgagtgcg atgaagatcg cgcacagggg gccagatgca
      181 tttcgctttg agaatgtcaa tggatacacc aactgctgct ttggctttca ccgcttggct
      241 gtggttgacc cgctgtttgg aatgcagccg ataagagtga ggaaataccc ttatttgtgg
      301 ctctgttaca atggtgaaat ctacaaccac aaggcgctac agcaacgttt tgaatttgaa
      361 tatcagacca atgtggatgg tgagattatc ctccacctct atgacaaagg aggcatcgag
      421 aaaaccatct gtatgctgga cggggtgttt gcattcatct tactggacac tgccaataag
      481 aaagtatttc tgggcagaga cacctatgga gtcaggccct tgtttaaagc catgacagaa
      541 gatgggtttc tggctgtgtg ttcagaagct aaaggccttg tttccttaaa acactccacc
      601 actcccttct taaaagtgga gcccttcctt cctggacact atgaagtttt ggatttaaaa
      661 ccaaatggca aagttgcgtc tgtggaaatg gtcaaatacc atcactgtac ggatgaacca
      721 ttgcatgcca tctatgacag cgtggagaaa ctcttcccag gctttgacct agagaccgtg
      781 aagaacaatc tgcgtatcct ttttgacaac gctatcaaga aacgcttgat gacagaccgg
      841 aggattggct gccttttatc agggggcctg gactcgagct tggttgctgc ctctctgctg
      901 aagcaactca aggaggccca agttcagtat cctctccaga catttgctat tggcatggag
      961 gacagccccg atctcctggc cgctagaaag gtggcaaatt atattggaag cgagcatcat
     1021 gaagtccttt ttaactctga agaaggcatt caggccctgg atgaagtcat attttccttg
     1081 gaaacttatg atattacgac agttcgggca tctgtgggca tgtatttaat ttccaagtat
     1141 attcggaaga acacagacag cgtggtgatc ttctccggag aggggtcaga tgaacttaca
     1201 cagggctata tatatttcca caaggctcct tcccctgaga aggccgagga ggagagtgag
     1261 agactgctga aggaactcta cctgtttgat gttctccggg ccgaccgcac tactgctgca
     1321 cacggtctcg aactgagagt cccctttctg gatcatcggt tttcttccta ttacctgtct
     1381 ctgccgccag atatgagaat tccaaaaaat ggcatagaaa aacatctcct gagagagact
     1441 tttgaggact gcaacctgct acccaaagag attctctggc gacccaaaga agccttcagt
     1501 gatgggatca cctcagtcaa gaactcctgg ttcaagattt tgcaggacta tgttgaacat
     1561 caggttgatg atgagatgat gtctgcagcc tcccagaagt ttcccttcaa tactcccaaa
     1621 actaaggaag gctacttcta ccgtcagatc tttgaacgcc attacccagg ccgggctgat
     1681 tggctgactc attattggat gcctaagtgg atcaatgcta ctgacccttc tgcccgcact
     1741 ctgacccatt ataagtcagc tgccaaagct taggcactct ctacactctt gtgtaaaagt
     1801 aaatgtttct tccggctctg aaggtcgaga cagcgacaca atcagaaaga atgagactca
     1861 gccatcagtc acccaggctt acttaggcat gaaaagaaat aaaagtttca catctgaaa
//
