LOCUS       NM_001410605            2243 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus asparagine synthetase (Asns), transcript variant 2,
            mRNA.
ACCESSION   NM_001410605 XM_006505093
VERSION     NM_001410605.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   3  (bases 1 to 2243)
  AUTHORS   Zhu,Y., Ackers-Johnson,M., Shanmugam,M.K., Pakkiri,L.S., Drum,C.L.,
            Chen,Y., Kim,J., Paltzer,W.G., Mahmoud,A.I., Tan,W.L.W.,
            Lee,M.C.J., Jiang,J., Luu,D.A.T., Ng,S.L., Li,P.Y.Q., Wang,A.,
            Qi,R., Ong,G.J.X., Ng,T.Y., Haigh,J.J., Tiang,Z., Richards,A.M. and
            Foo,R.S.Y.
  TITLE     Asparagine Synthetase Marks a Distinct Dependency Threshold for
            Cardiomyocyte Dedifferentiation
  JOURNAL   Circulation 149 (23), 1833-1851 (2024)
   PUBMED   38586957
  REMARK    GeneRIF: Asparagine Synthetase Marks a Distinct Dependency
            Threshold for Cardiomyocyte Dedifferentiation.
REFERENCE   4  (bases 1 to 2243)
  AUTHORS   Clerici,S., Podrini,C., Stefanoni,D., Distefano,G., Cassina,L.,
            Steidl,M.E., Tronci,L., Canu,T., Chiaravalli,M., Spies,D.,
            Bell,T.A. 3rd, Costa,A.S., Esposito,A., D'Alessandro,A., Frezza,C.,
            Bachi,A. and Boletta,A.
  TITLE     Inhibition of asparagine synthetase effectively retards polycystic
            kidney disease progression
  JOURNAL   EMBO Mol Med 16 (6), 1379-1403 (2024)
   PUBMED   38684863
  REMARK    GeneRIF: Inhibition of asparagine synthetase effectively retards
            polycystic kidney disease progression.
REFERENCE   5  (bases 1 to 2243)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 2243)
  AUTHORS   Ruzzo,E.K., Capo-Chichi,J.M., Ben-Zeev,B., Chitayat,D., Mao,H.,
            Pappas,A.L., Hitomi,Y., Lu,Y.F., Yao,X., Hamdan,F.F., Pelak,K.,
            Reznik-Wolf,H., Bar-Joseph,I., Oz-Levi,D., Lev,D., Lerman-Sagie,T.,
            Leshinsky-Silver,E., Anikster,Y., Ben-Asher,E., Olender,T.,
            Colleaux,L., Decarie,J.C., Blaser,S., Banwell,B., Joshi,R.B.,
            He,X.P., Patry,L., Silver,R.J., Dobrzeniecka,S., Islam,M.S.,
            Hasnat,A., Samuels,M.E., Aryal,D.K., Rodriguiz,R.M., Jiang,Y.H.,
            Wetsel,W.C., McNamara,J.O., Rouleau,G.A., Silver,D.L., Lancet,D.,
            Pras,E., Mitchell,G.A., Michaud,J.L. and Goldstein,D.B.
  TITLE     Deficiency of asparagine synthetase causes congenital microcephaly
            and a progressive form of encephalopathy
  JOURNAL   Neuron 80 (2), 429-441 (2013)
   PUBMED   24139043
REFERENCE   7  (bases 1 to 2243)
  AUTHORS   Iida,S., Kamiya,H., Nakaya,A., Hayashi,Y., Sawada,A., Kaji,N.,
            Baba,Y. and Harashima,H.
  TITLE     Reduced plasma glucose by asparagine synthetase knockdown in the
            mouse liver
  JOURNAL   Biol Pharm Bull 36 (12), 2009-2011 (2013)
   PUBMED   24292060
  REMARK    GeneRIF: Asns is involved in the regulation of plasma glucose
            levels.
REFERENCE   8  (bases 1 to 2243)
  AUTHORS   Seo,J., Fortuno,E.S. 3rd, Suh,J.M., Stenesen,D., Tang,W.,
            Parks,E.J., Adams,C.M., Townes,T. and Graff,J.M.
  TITLE     Atf4 regulates obesity, glucose homeostasis, and energy expenditure
  JOURNAL   Diabetes 58 (11), 2565-2573 (2009)
   PUBMED   19690063
REFERENCE   9  (bases 1 to 2243)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   10 (bases 1 to 2243)
  AUTHORS   Van Dyck,F., Braem,C.V., Chen,Z., Declercq,J., Deckers,R.,
            Kim,B.M., Ito,S., Wu,M.K., Cohen,D.E., Dewerchin,M., Derua,R.,
            Waelkens,E., Fiette,L., Roebroek,A., Schuit,F., Van de Ven,W.J. and
            Shivdasani,R.A.
  TITLE     Loss of the PlagL2 transcription factor affects lacteal uptake of
            chylomicrons
  JOURNAL   Cell Metab 6 (5), 406-413 (2007)
   PUBMED   17983586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129291.4.
            
            On Aug 13, 2022 this sequence version replaced XM_006505093.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.141295.1,
                                           SRR7345562.1322294.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-388               AC129291.4         86837-87224
            389-660             AC129291.4         90224-90495
            661-898             AC129291.4         94341-94578
            899-1084            AC129291.4         97291-97476
            1085-1186           AC129291.4         97712-97813
            1187-1314           AC129291.4         98370-98497
            1315-1441           AC129291.4         99536-99662
            1442-1548           AC129291.4         101701-101807
            1549-1649           AC129291.4         102416-102516
            1650-1731           AC129291.4         103431-103512
            1732-1887           AC129291.4         103599-103754
            1888-2243           AC129291.4         104223-104578
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 3.31 cM"
     gene            1..2243
                     /gene="Asns"
                     /note="asparagine synthetase"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
     exon            1..388
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            389..660
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     CDS             412..2097
                     /gene="Asns"
                     /EC_number="6.3.5.4"
                     /note="isoform a is encoded by transcript variant 2;
                     glutamine-dependent asparagine synthetase"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]
                     isoform a"
                     /protein_id="NP_001397534.1"
                     /db_xref="GeneID:27053"
                     /db_xref="MGI:MGI:1350929"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVRKYPYLWLCYNGEIYNHKALQQRFEFEYQTNVDGEIIL
                     HLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
                     AKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDS
                     VEKLFPGFDLETVKNNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKE
                     AQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLKELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPDMRIPKNGIEKHLLRE
                     TFEDCNLLPKEILWRPKEAFSDGITSVKNSWFKILQDYVEHQVDDEMMSAASQKFPFN
                     TPKTKEGYFYRQIFERHYPGRADWLTHYWMPKWINATDPSARTLTHYKSAAKA"
     exon            661..898
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1084
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1186
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1314
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1441
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1548
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1649
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1731
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1887
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2243
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2222..2227
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asns"
                     /note="hexamer: AATAAA"
     polyA_site      2243
                     /gene="Asns"
                     /note="major polyA site"
ORIGIN      
        1 aggaaagctt tcatgtgagc gaagtgcagc gcggaggcag ggccgctcca gacagaaggc
       61 gaaggtggcc ttcatttaga caccataaat attttttttt actgtatgac gtctagatct
      121 gggaaatttc agccatccga aggttttgcc tgggactagg tggacgcttg cacaagacca
      181 gctgtaattg ggagccccag gctagtgtag gggtctcgct ctccagcagg acagaggctc
      241 taccaagctc tagagcgagt gtctctgggt ctgtctggat ccggatttct gagcactcac
      301 tcacctcgct gctcaggccc ttcctccact ccagttccat actcgcagcc acgccgggaa
      361 aaatctgttt aaggagacag gcaaccaaag tgcctgcagt ccgcctgtag catgtgtggc
      421 atctgggccc tcttcggcag cgatgactgc ctttccgtgc agtgtctgag tgcgatgaag
      481 atcgcgcaca gggggccaga tgcatttcgc tttgagaatg tcaatggata caccaactgc
      541 tgctttggct ttcaccgctt ggctgtggtt gacccgctgt ttggaatgca gccgataaga
      601 gtgaggaaat acccttattt gtggctctgt tacaatggtg aaatctacaa ccacaaggcg
      661 ctacagcaac gttttgaatt tgaatatcag accaatgtgg atggtgagat tatcctccac
      721 ctctatgaca aaggaggcat cgagaaaacc atctgtatgc tggacggggt gtttgcattc
      781 atcttactgg acactgccaa taagaaagta tttctgggca gagacaccta tggagtcagg
      841 cccttgttta aagccatgac agaagatggg tttctggctg tgtgttcaga agctaaaggc
      901 cttgtttcct taaaacactc caccactccc ttcttaaaag tggagccctt ccttcctgga
      961 cactatgaag ttttggattt aaaaccaaat ggcaaagttg cgtctgtgga aatggtcaaa
     1021 taccatcact gtacggatga accattgcat gccatctatg acagcgtgga gaaactcttc
     1081 ccaggctttg acctagagac cgtgaagaac aatctgcgta tcctttttga caacgctatc
     1141 aagaaacgct tgatgacaga ccggaggatt ggctgccttt tatcaggggg cctggactcg
     1201 agcttggttg ctgcctctct gctgaagcaa ctcaaggagg cccaagttca gtatcctctc
     1261 cagacatttg ctattggcat ggaggacagc cccgatctcc tggccgctag aaaggtggca
     1321 aattatattg gaagcgagca tcatgaagtc ctttttaact ctgaagaagg cattcaggcc
     1381 ctggatgaag tcatattttc cttggaaact tatgatatta cgacagttcg ggcatctgtg
     1441 ggcatgtatt taatttccaa gtatattcgg aagaacacag acagcgtggt gatcttctcc
     1501 ggagaggggt cagatgaact tacacagggc tatatatatt tccacaaggc tccttcccct
     1561 gagaaggccg aggaggagag tgagagactg ctgaaggaac tctacctgtt tgatgttctc
     1621 cgggccgacc gcactactgc tgcacacggt ctcgaactga gagtcccctt tctggatcat
     1681 cggttttctt cctattacct gtctctgccg ccagatatga gaattccaaa aaatggcata
     1741 gaaaaacatc tcctgagaga gacttttgag gactgcaacc tgctacccaa agagattctc
     1801 tggcgaccca aagaagcctt cagtgatggg atcacctcag tcaagaactc ctggttcaag
     1861 attttgcagg actatgttga acatcaggtt gatgatgaga tgatgtctgc agcctcccag
     1921 aagtttccct tcaatactcc caaaactaag gaaggctact tctaccgtca gatctttgaa
     1981 cgccattacc caggccgggc tgattggctg actcattatt ggatgcctaa gtggatcaat
     2041 gctactgacc cttctgcccg cactctgacc cattataagt cagctgccaa agcttaggca
     2101 ctctctacac tcttgtgtaa aagtaaatgt ttcttccggc tctgaaggtc gagacagcga
     2161 cacaatcaga aagaatgaga ctcagccatc agtcacccag gcttacttag gcatgaaaag
     2221 aaataaaagt ttcacatctg aaa
//
