LOCUS       NM_138679              11293 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus ASH1 like histone lysine methyltransferase (Ash1l),
            mRNA.
ACCESSION   NM_138679
VERSION     NM_138679.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Shipman,G.A., Padilla,R., Horth,C., Hu,B., Bareke,E.,
            Vitorino,F.N., Gongora,J.M., Garcia,B.A., Lu,C. and Majewski,J.
  TITLE     Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and ASH1L
            reveal their distinct contributions to H3K36 methylation
  JOURNAL   Genome Biol 25 (1), 263 (2024)
   PUBMED   39390582
  REMARK    GeneRIF: Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and
            ASH1L reveal their distinct contributions to H3K36 methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   Zhao,X., Lin,S., Ren,H., Sun,S., Zheng,L., Chen,L.F. and Wang,Z.
  TITLE     The histone methyltransferase ASH1L protects against bone loss by
            inhibiting osteoclastogenesis
  JOURNAL   Cell Death Differ 31 (5), 605-617 (2024)
   PUBMED   38431690
REFERENCE   3  (bases 1 to 11293)
  AUTHORS   Du,W.J., Yang,H., Tong,F., Liu,S., Zhang,C., Chen,Y., Yan,Y.,
            Xiang,Y.W., Hua,L.Y., Gong,Y., Xu,Z.X., Liu,X., Jiang,X., Lu,M.,
            Guan,J.S. and Han,Q.
  TITLE     Ash1L ameliorates psoriasis via limiting neuronal
            activity-dependent release of miR-let-7b
  JOURNAL   Br J Pharmacol 181 (7), 1107-1127 (2024)
   PUBMED   37766518
  REMARK    GeneRIF: Ash1L ameliorates psoriasis via limiting neuronal
            activity-dependent release of miR-let-7b.
REFERENCE   4  (bases 1 to 11293)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 11293)
  AUTHORS   Zhang,T., Ren,T., Lin,H., Tong,Y., Zhang,J., Nie,J., Zhu,Y.,
            Wang,Y., Jin,B., Zhang,C., Chen,T. and He,M.
  TITLE     ASH1L contributes to oocyte apoptosis by regulating DNA damage
  JOURNAL   Am J Physiol Cell Physiol 323 (4), C1264-C1273 (2022)
   PUBMED   36094439
  REMARK    GeneRIF: ASH1L contributes to oocyte apoptosis by regulating DNA
            damage.
REFERENCE   6  (bases 1 to 11293)
  AUTHORS   Tanaka,Y., Katagiri,Z., Kawahashi,K., Kioussis,D. and Kitajima,S.
  TITLE     Trithorax-group protein ASH1 methylates histone H3 lysine 36
  JOURNAL   Gene 397 (1-2), 161-168 (2007)
   PUBMED   17544230
  REMARK    GeneRIF: ASH1 mono- or di-methylates histone H3 K36 but not any
            other lysine residues of recombinant unmodified mammalian histones
REFERENCE   7  (bases 1 to 11293)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   8  (bases 1 to 11293)
  AUTHORS   Ding,S., Wu,X., Li,G., Han,M., Zhuang,Y. and Xu,T.
  TITLE     Efficient transposition of the piggyBac (PB) transposon in
            mammalian cells and mice
  JOURNAL   Cell 122 (3), 473-483 (2005)
   PUBMED   16096065
REFERENCE   9  (bases 1 to 11293)
  AUTHORS   Zucchelli,S., Holler,P., Yamagata,T., Roy,M., Benoist,C. and
            Mathis,D.
  TITLE     Defective central tolerance induction in NOD mice: genomics and
            genetics
  JOURNAL   Immunity 22 (3), 385-396 (2005)
   PUBMED   15780994
REFERENCE   10 (bases 1 to 11293)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK627544.1, CF167627.1, AK153783.1, BC052194.1, AK033177.1 and
            AK031724.1.
            
            On Sep 22, 2005 this sequence version replaced NM_138679.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-622               CK627544.1         22-643
            623-821             CF167627.1         198-396
            822-6113            AK153783.1         1-5292
            6114-6426           BC052194.1         31-343
            6427-6436           AK033177.1         188-197
            6437-9290           BC052194.1         354-3207
            9291-10772          AK031724.1         533-2014
            10773-11293         BC052194.1         4692-5212
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.01 cM"
     gene            1..11293
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /note="ASH1 like histone lysine methyltransferase"
                     /db_xref="GeneID:192195"
                     /db_xref="MGI:MGI:2183158"
     exon            1..517
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..8977
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /EC_number="2.1.1.359"
                     /EC_number="2.1.1.367"
                     /note="probable histone-lysine N-methyltransferase ASH1L;
                     ASH1-like protein; absent small and homeotic disks protein
                     1 homolog; absent, small, or homeotic discs 1; chromatin
                     remodeling factor; ash1 (absent, small, or homeotic)-like"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase ASH1L"
                     /protein_id="NP_619620.3"
                     /db_xref="CCDS:CCDS17487.1"
                     /db_xref="GeneID:192195"
                     /db_xref="MGI:MGI:2183158"
                     /translation="MDPRNTAMLGLGSDSEGFSRKSPSTINPGTLASKREAEIEGATE
                     EEDPRKRNRERGTEAGKEDGSTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNL
                     ENYVCRPAIKTTIKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIDCQAEE
                     SIHLHSQGESNPLSKKLSPVHSQMADYISAAPSLVGSRDPDIKDRALLNGGTSVTEKL
                     AQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALST
                     AVGLVTKDPGKKPMFNAAVGLINKDSVKKLGTGTTAVFINKDLGKKPGAITTVGLLSK
                     ESGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVAS
                     SAMGLVNKDIGKKLLNCPMAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHDSQE
                     LPESLKDSATGKAFEKSVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDNFSSS
                     ERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFSAVGESNLPSSSPTVSVNPV
                     TRSPPEASSQLVPNPLLLNSTAEQMEEISESIGKSQFTAESTHLNVGHRSLGHSLSIE
                     CKGIDKELNESKNTHLDIPRISSSLGKKPSLTSDSGIHAITPSVVNFTSLFSNKPFLK
                     LGAVTAPDKHCQVAESLSSSFQSKPLKKRKGRKPRWTKVVARSTCRSPKGLDLERSEL
                     FKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLTDSEKPS
                     HKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQ
                     SKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVAT
                     ESPSKLESESENHRSSSDFFESEDQLQDTDDLDDSHRQSVCSMSDLEMEPDKKISKRN
                     NGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSK
                     LGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILP
                     PLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQT
                     LAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTD
                     NNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDK
                     MKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRI
                     NFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYP
                     SYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTK
                     YHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQ
                     PQVSMDSGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKNLINREEQ
                     WVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGS
                     CRVSNPNSSCRKKLTDSPGLFPVQDTALNRPHRKEPLPSSERAIQSLAGSQSASDKPS
                     QRSSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASMDDSVDSLLQRIVHHDEQES
                     MEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSAS
                     RCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFV
                     KRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVN
                     LTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIETPVEIPSPLETPAEPSE
                     PENTLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTIDPKSRLIQLKKEKLEYTPG
                     EHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVD
                     VKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHE
                     WVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYC
                     LNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTY
                     DYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHKGSQSSSTHRKSARAKEKR
                     KSKHKLKKRKGHPSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKI
                     HQKQEEVKHTRDIHSASLYTRWNGLCRDDGNIKSDVFMTQFSALQTARSVRTRRLAAA
                     EENLEVARAARLAQIFKEICDGIISYRDSSQQTLAAPLLNLPPKKKNADYYEKISDPL
                     DLSTIEKQILIGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYSARHE
                     ASAQIDEIVGETASEADSSETSVSEKESGHEKDDDVIRCICGLYKDEGLMIQCDKCMV
                     WQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQ
                     GDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYF
                     RPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGIKEQDVY
                     ICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGR
                     SSWKSERSKPPLKDLGQEDDALPLIEEVLASQEQAAREVPSPEEPDQERATGDIGDAE
                     KKPEESSQEAQLASTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKL
                     RRRTLFIPENSFRK"
     exon            518..5075
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            5076..5177
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            5178..5913
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            5914..6093
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6094..6188
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6189..6262
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6263..6308
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6309..6417
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6418..6624
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6625..6771
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6772..6880
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            6881..7045
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7046..7140
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7141..7295
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7296..7448
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7449..7503
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7504..7636
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7637..7885
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7886..7972
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            7973..8063
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8064..8202
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8203..8375
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8376..8542
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8543..8583
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8584..8885
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     exon            8886..11270
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11246..11251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /note="hexamer: ATTAAA"
     polyA_site      11270
                     /gene="Ash1l"
                     /gene_synonym="8030453L17Rik; Ash1; E430018P19Rik; Kmt2h"
                     /note="major polyA site"
ORIGIN      
        1 ggaaagagat gctggttgca agttttggaa aaccctattt ttttatggaa cagtgttctg
       61 taaaagtttt gtaagatccc ttggcaataa cacacttgtg atggacccta ggaatactgc
      121 tatgttagga ttgggttctg attccgaagg attttcaaga aagagtcctt ctaccatcaa
      181 tcctggcact ctggccagca aaagagaagc agagatagag ggtgccacag aagaagagga
      241 tcctcgcaag cggaatcgag aaaggggcac agaagcagga aaggaggatg gctcgacgga
      301 tgcacagcaa cagttttcag tgaaagaaac aaacttttca gagggaaact taaaattgaa
      361 aattggcctt caggcaaaga gaactaaaaa gcctccaaag aacttggaga actatgtgtg
      421 tcgacctgcc ataaagacaa ccatcaagca ctcaaggaaa gcacttaaaa gtgggaagat
      481 gacagatgag aagaatgagc actgcccttc aaaatgggac tcttcaaaat tgttcaagaa
      541 agcaggtgac gctacagcca tcgactgcca agccgaagaa agcatccatc ttcattcaca
      601 gggggaaagt aatcctttgt ctaagaagct ttctccagta cattcacaaa tggcagatta
      661 cattagtgca gcaccatctc ttgttggcag tcgagatcct gatataaagg acagagcatt
      721 acttaatgga ggaactagtg taacagaaaa gttggcacag ctcattgcta cctgtcctcc
      781 ttccaagtct tccaaggcaa aaccgaagaa gttaggaact ggcactacag ttggattggt
      841 tagcaaggac ttgatcagga aaccaggtgt tggctctata gctggaataa ttcataaaga
      901 cttaattaaa aagccagctc ttagcacagc agttgggttg gtaactaaag atccagggaa
      961 aaagcctatg ttcaatgcag cagtaggcct gatcaataag gactctgtga aaaaattggg
     1021 aactggcact acagccgtat tcattaataa agacttaggc aaaaagccag gggctatcac
     1081 tacagttgga ctgctgagca aagaatcagg aaagaagcta ggcattggta ttgttccagg
     1141 gctagtgaat aaagagtctg gcaagaaatt aggacttggc accgtagttg gactggttaa
     1201 taaagagttg ggaaagaaac tgagttctac agttggccta gtggccaagg atgttacgaa
     1261 gaagattgta gcaagctcag caatgggatt ggttaataag gacattggaa agaaattatt
     1321 aaattgtcct atggcaggac aactgggcag taaagatgcc ttaaacctta aatctgaagc
     1381 actgctcccc actcaggaac aacttaaggc ttcctgtagt gcaaacataa gtaatcatga
     1441 cagtcaagaa cttcctgagt ctctgaaaga cagtgctacc ggcaaagctt ttgagaagag
     1501 tgttatgcgg catagtaaag aaagcatgtt ggaaaaattt tcagtccgaa aagaaattac
     1561 taatttggag aaagaaatgt ttaatgaagg aacatgcatt cagcaagaca atttctcatc
     1621 tagtgaaagg ggagcctttg aaacctcaaa acatgaaaag cagcctcctg tgtattgcac
     1681 ttctcctgac tttcaaatag gaggtgcctc tgatgcctct acagctaaat ctcccttcag
     1741 tgcagtaggc gaaagcaacc ttccttcctc gtcacctact gtatctgtta atcctgtaac
     1801 caggagtccc cctgaagctt cttcacagtt ggttccaaac ccattacttt taaattctac
     1861 agcagaacaa atggaagaaa tctctgaatc tattggaaag agccagttta ctgctgaaag
     1921 tacccacttg aatgttggtc acagatcatt aggtcatagc ttaagtattg agtgtaaagg
     1981 aattgataaa gaactaaatg aatcaaaaaa tacacatcta gatattccta gaataagttc
     2041 ttccttggga aaaaagccaa gtttgacttc tgactctggt attcatgcaa ttactccttc
     2101 cgttgttaat tttactagtt tgtttagtaa caaacctttt ttaaagcttg gtgcagtcac
     2161 tgcaccagac aaacactgcc aggttgctga aagcctaagc tcgagtttcc agtccaaacc
     2221 attgaaaaaa agaaaaggaa gaaaacctcg gtggactaaa gtggtggcaa gaagcacatg
     2281 ccggtctcca aaaggactag acttagaaag atcagagctt tttaagaatg tttcttgtag
     2341 ctcactatca aatagttctg agccagcgaa gtttatgaaa actatcggag catcatcatt
     2401 cgtcgatcat gacttcctta aacgccgatt gccaaagttg agtaagtctt cagctccatc
     2461 tcttgctctt ttaaccgaca gcgagaaacc atctcataag tcttttatta ctcacaaact
     2521 gtcctccagt atgtgtgtca ctagcgatct tttgtcggat atttataagc ccaagagagg
     2581 aagacctaaa tccaaggaaa tgcctcagct agaaggtcca cctaaaagga ctttaaaaat
     2641 acctgcctct aaagtttttt ctttacagtc taaagaagag caagaacccc caattttaca
     2701 accagaaatt gaaattcctt ccttcaagca aagtttgtct gtatctcctt ttccaaaaaa
     2761 aagaggcagg cctaagaggc agatgaggtc tccagtgaag atgaaacccc ctgtcctgtc
     2821 agtggctcca tttgttgcca ctgaaagtcc aagtaagctc gagtctgaaa gtgagaacca
     2881 tagaagtagc agtgactttt ttgagagtga ggaccagctt caggacacag acgacttaga
     2941 tgacagtcac aggcaaagtg tctgtagtat gagtgacctt gagatggaac cagataaaaa
     3001 aattagcaag cgaaacaatg gacaattaat gaaaacaatt atccgaaaaa taaataaaat
     3061 gaagacttta aagagaaaaa aactgttgaa tcagattctt tcgagttctg tagaatcaag
     3121 taataaagga aaagtgcagt ccaagcttca caatacagtc tcaagtcttg ctgccacatt
     3181 tggctctaaa ttggggcaac aaatcaatgt cagcaagaaa ggaaccattt acatagggaa
     3241 gagaaggggg cggaaaccaa aaaccgtctt aaatggcctg ctttctggta gccctgccag
     3301 ccttgctgtg cttgaacaaa cagctcagca ggcagctggg tcagcattag gacagatcct
     3361 ccccccttta ctgccatcac ctgctagtag ctctgagatc cttccatcac ctatttgctc
     3421 tcagtcttct gggactagtg gaggtcagag ccctgttagt agtgacgcag gctttgttga
     3481 acctagctca gtgccatatt tgcatgtgca ctccagacag ggcagtatga ttcagactct
     3541 tgcaatgaag aaggcttcca agggcagaag gcggctctct cctcctactt tgttgccaaa
     3601 ttctccttct catttgagtg aactcacatc cctgaaagaa gccactcctt ccccagttag
     3661 tgagtctcat agtgacgaga ccattcccag tgacagtggc attgggacag ataataatag
     3721 cacatcagac agggcagaga agttttgtgg acagaaaaag aggagacatt cttttgagca
     3781 catttctctg attccccctg aaacttctac cgtcctaaac agtctcaaag aaaaacacaa
     3841 acacaaatgt aagcgcagga gtcacgatta cctcagctat gacaagatga aaagacagaa
     3901 gcgaaaacgg aaaaagaaat atccccagct tcgaaatagg caggatccag acttcatcgc
     3961 agaactagag gagctgataa gtcgtctaag tgagattcgg atcacccatc gaagccatca
     4021 ttttattccc cgagacctcc tgccaactat ttttcgaatc aactttaata gtttctatac
     4081 acatccttct tttcccttag acccattgca ctacattcgg aaacctgact taaaaaagaa
     4141 acgagggagg ccccctaaga tgagggaggc aatggcagaa atgcctttta tgcacagcct
     4201 tagttttcct ttatctagta ctggatttta tccttcttat ggcatgcctt actctccctc
     4261 accccttaca gctgctccaa taggattagg gtattatgga aggtatcccc ccactcttta
     4321 tccaccgcca ccatctcctt cattcaccac tccactgcca cctccctcct atatgcatgc
     4381 tggtcattta ttgctcaatc ctaccaaata tcataagaaa aagcataagc tgcttagaca
     4441 ggaggctttt cttacaacca gcaggacccc tctcctttcc atgagcacct accccagcgt
     4501 acctccagag atggcttatg gttggatggt ggagcacaaa cacagacacc gtcacaaaca
     4561 cagagagcac cgttctgaac agccccaggt ttccatggat agtggctctt ccagatccgt
     4621 cttggagtct ttgaaacgtt accgatttgg taaggatact gttggagatc gatataagca
     4681 taaggaaaag cacaggtgtc acatgtcctg ccctcatctc tctccttcaa agaacttaat
     4741 aaacagagaa gaacagtggg tttctcgaga gccttcagaa tcaagttctt tagccttggg
     4801 attgcaaaca cctttacaaa ttgactgctc agaaagttct cccagtttgt cccttggagg
     4861 atttactccc aattctgagc cagccagcag tgatgaacat atgaaccttt ttacaagtgc
     4921 aataggcagc tgcagagttt caaaccctaa ctccagttgc cggaagaaat taactgacag
     4981 ccctgggctc tttcctgtac aagatactgc actaaatcgg cctcacagaa aggaaccatt
     5041 accttccagt gaaagggcaa tacagtcgtt ggcaggttcc cagtcagctt ccgataaacc
     5101 ttcccagcga tcatcagaaa gcacaaattg tagccctact cgtaaaaggt cttcatctga
     5161 gagtacttct tcaacagtga atggagttcc ttccagaagt cccagattgg ttgcttctat
     5221 ggatgattct gtagacagtc tgttgcagcg gatagtacat cacgatgagc aagagtccat
     5281 ggagaaaaac ggtgatgctt ccattacaac tgtgtctgca ccaccttctt ccagtccagg
     5341 ccatagctac agcaaggagc gagccttagg taaatcagac agccttctag tgcctgcagt
     5401 cccgaatgac tcttgcagta atatcccact tctttctgaa aagtcagcaa gtaggtgttc
     5461 cccccatcac atcaagagaa gtgtagttga agctatgcaa cgccaagctc ggaaaatgtg
     5521 caattatgac aaaatcttgg ccaccaagaa aaacctggac catgttaata aaattttaaa
     5581 agccaaaaaa cttcaaaggc aggccaggac aggaaacaat tttgtgaaac gcagaccagg
     5641 tcgacctcgg aaatgccccc tccaagcagt ggtgtcaatg caagccttcc aggccgccca
     5701 gtttgtcagc ccggaattga atgaaggcga agacatgtcc ctgcacctaa gtccagacac
     5761 agtcactgat gtgatcgagg ctgtggttca gagtgtgaac ctcacttcag aacataagaa
     5821 gggggtgaag aggaaaaatt ggctgttaga agaacagact aggaaaaagc agaagacagt
     5881 accagaggaa gaagagcaag aaaacaataa aagctttata gaaacaccag ttgaaattcc
     5941 cagtcctctt gaaacgcctg ctgaaccttc tgaacctgaa aataccttgc aacctgtgct
     6001 ggctctcatc ccacgagaaa agaaggcccc acgtcctcca aagaagaaat accagagagc
     6061 tgggctgtat tctgatgttt acaaaacaat agacccaaag agccgattaa tccaattaaa
     6121 gaaagaaaag ttggagtata ctccaggaga gcacgagtat gggttgttcc cagcgcccat
     6181 tcatgttgga aagtatctca gacagaagag aattgacttc cagcttccct atgatattct
     6241 ctggcagtgg aaacacaatc agctatacaa aaagccagat gtccccctgt ataaaaaaat
     6301 ccgttcaaat gtctatgttg atgtcaaacc actttctggt tatgaggcta ccacctgtaa
     6361 ttgtaagaag ccagatgacg acaccaggaa gggctgtggg gatgactgcc taaatagaat
     6421 gatctttgct gagtgttccc ctaacacttg cccctgtggt gagcagtgct gtaaccagag
     6481 gatacagagg catgagtggg tgcagtgtct ggaacgattt cgagctgagg aaaaaggttg
     6541 gggaattaga accaaagaac ccctaaaagc tggccagttc atcattgaat acctagggga
     6601 agttgtcagc gaacaagaat tcaggaacag gatgattgag caatatcata atcacagtga
     6661 ccactactgt ttaaacctgg atagcggaat ggtgattgac agttaccgca tgggaaatga
     6721 ggccagattc atcaaccaca gctgtgaccc aaattgtgaa atgcagaaat ggtctgttaa
     6781 tggagtgtac cgtattggac tgtatgcact taaggatatg ccagctggga cagagcttac
     6841 ctatgattac aactttcatt ccttcaatgt agaaaaacag caactttgta aatgtggttt
     6901 tgagaagtgt cgaggaatca ttggaggcaa gagtcaacgg atgaacggac tccctagcca
     6961 taaaggtagc cagtcttcaa gcacgcatag aaagtctgca cgggcgaaag agaagcggaa
     7021 gtccaagcac aagctgaaga aaaggaaagg ccatccctct gaagagccca gtgaaaacat
     7081 caacacccca acaagattga ctccacagtt acagatgaag ccaatgtcca acagagagag
     7141 gaacttcgtg ctaaagcacc atgtgttctt ggtgcgaaac tgggaaaaga tccatcagaa
     7201 gcaggaggag gtgaagcaca cccgtgacat ccactcagca tcgctgtaca cccgctggaa
     7261 cggcctctgt cgggacgacg ggaacattaa gtctgatgtc ttcatgaccc agttctctgc
     7321 cctgcagaca gctagatctg ttcgaacaag acgcttggca gctgcagagg aaaacctgga
     7381 agtggctcgg gcagcgcgcc tagcacagat cttcaaagag atctgtgatg gcatcatctc
     7441 ctacagagat tcttcccagc aaacccttgc agctccactt ttgaatcttc ccccaaagaa
     7501 aaaaaatgct gactattatg agaagatctc tgaccccctg gatctcagca ccatagagaa
     7561 gcagatcctc attgggtatt acaaaacagt ggaggctttc gatgctgaca tgcttaaggt
     7621 ctttcggaat gctgagaagt actatgggcg taaatcccca attgggagag atgtttgccg
     7681 cttacgaaag gcctattaca gcgcccggca tgaagcttcg gctcagatcg atgagattgt
     7741 gggagaaacg gccagtgagg ccgacagcag cgagacctca gtctccgaga aggagagcgg
     7801 gcacgagaag gatgatgatg ttatccgctg catctgcggc ctctataaag atgaaggcct
     7861 catgatccag tgtgacaagt gcatggtgtg gcagcactgc gactgcatgg gtgtgaacac
     7921 ggatgtggag cactaccttt gtgagcagtg tgaccctcgg cctgtggaca gggaggtacc
     7981 catgatccct aggccccact atgcccagcc cggctgtgtc tacttcatct gtttactccg
     8041 agatgacttg ctgcttcgtc agggtgattg tgtgtacctg atgagggaca gtcggcgcac
     8101 gcctgatggc cacccagtcc gtcagtccta cagactctta tctcacatta accgagataa
     8161 acttgacatc tttagaatcg agaagctctg gaagaatgaa aaagaagaac ggtttgcctt
     8221 tggtcaccat tatttccgcc cacatgagac tcatcactct ccatcccgtc ggttctatca
     8281 caacgagctg ttccgggtgc ccctctatga gatcattccc ttggaggccg tagttgggac
     8341 ctgctgtgtg ttggaccttt atacatactg taaagggaga cccaaaggaa taaaggaaca
     8401 agatgtgtat atctgtgatt atcggcttga caagtcagca cacctttttt acaagattca
     8461 tcggaatcga tatccggtct gcaccaaacc ctatgccttt gatcatttcc ccaagaagct
     8521 cactcccaaa agagacttct cacctcatta tgtcccagac aactacaaga ggaacggagg
     8581 acgatcatcc tggaagtctg aacgctcaaa gccaccccta aaagacctag gacaagaaga
     8641 tgatgctttg cccttgattg aagaggtact ggctagtcag gagcaagcag ccagggaagt
     8701 gcccagccct gaggagccag accaggaaag agccactggg gacattgggg acgctgaaaa
     8761 gaaaccagag gagagcagtc aagaagccca gttagccagc acacctgagg agcgaaggca
     8821 cagccagagg gaacggctca accagatcct gctcaacctc cttgagaaaa tacctgggaa
     8881 aaatgccatt gacgtgacct acctgctgga ggaagggtcc ggcaggaaac tgcgaaggcg
     8941 cactttgttt ataccagaaa atagctttcg gaagtgactc tcaaaaggat gagaaccgcg
     9001 agcgagcacc tgggatccaa cggcaccatc aagtccagcc tcttgctctc tggacatgga
     9061 ctggggcaga aaagttctgt tcaggcaagg ggggtggggt cacgtttctg acatggtact
     9121 ttctaaagct cggagctagt ggaaagggaa aggaagtagg cttgtctgag acgggtgcgt
     9181 ttacttctct ctctggcttc tcctgagggt taacagagac aggaggacca cacttggagt
     9241 agccagaagc tactggtact ttccagcact tccaaaggga agggtccgga cccagcctgc
     9301 cccgtttatc tcttagctct aagagctacg tcagagacct aaacaggtac agtagctgag
     9361 gcctctttta agttccttta acgggaaagt tctttctccc ttcttgccca accaggtcaa
     9421 agtactagaa catgaatcag ctgctcagag cacttagaac tgctgccact ctccctacct
     9481 ctgttcccag agtagagtca gtagaaccag ggaggcggag aggcccccat ggggtccgaa
     9541 gggcagtatt ggtaattatt tttggtttac ccttcataac ctgccgaaca tactttttcc
     9601 tcctgagaaa gcccagcccc cgccagcaca catgctgctt ctcttcctgt gtgtctgctg
     9661 tgcaaaagat tcacgtccaa gaaagaaaaa cggtttgtaa aactgcataa accctctaca
     9721 cttctcccct aacaaaactc actacagaac tgttgagcag tgttcactct cggagtgttt
     9781 gttctaagtg taggttaccg actgaaacac tactcttatt ttcttaactg tatagttccg
     9841 gtgtccgtcc ctcccacgag tcccattttc cttccacctt tctgaatgac ttcatcatca
     9901 ggaggtatag ccagggtcac cttctcaccc agtctcttga ccagaagtta ccagaccata
     9961 ctgtctcttt acaagtaaaa ttcaaaaagc tttgctttgt cttctcagac atacatatgc
    10021 atatacattt tagatgttct tataagagaa aagatggttt ttaaatgtgc caagttatgt
    10081 gtgtgtataa atatatatat gtgtgtgtgt gcgtgtatat atatatatgt gtgtgtgtgt
    10141 atgtgtatat atatatgtgt gtgtgggtgc gagtgtgcga gtatgtgtat gtgggggtgg
    10201 gtgcgcgtgt gtgtgtgtgt gtgtatgctg tgagattggt aagcaataca ttagtaaaca
    10261 catcttcatt actcttttcc aatactggac caatgatgtc ctaactgtac atttccttcc
    10321 ccttatgatg acgacgctct gactcattta ggtagagaca tttgaccacc ttcattccat
    10381 tgattttttt tttctccttt ctgtgttatt cttaagggaa aagagagaga gagaaagagg
    10441 ggatggcata gatccccttg agcagagaaa aagcaaaata ttttattaaa gaaaaaaaga
    10501 aattaagaaa atagtttgga gtattttctt actgtagaga agcattgtac attaccacga
    10561 gacctgggta tgagatgctc gcgcgtggcg ctggggagcg catggccaag cccactggag
    10621 tggtttcttt tgtttctcat tgcagggagg gggagggggg aggactaagg gcactttggg
    10681 ggtctccttt tagtcaaaag caagaaaatg acaagaaaga gattaaattc aatgttttcc
    10741 tttaatagtg ttaaacacta aaattttaaa ataagaaaga aaaaactttg taaaatgtaa
    10801 gaacagaagc aaaagacact acgctgtcat ttttatcttt cttttgttga aagattaaaa
    10861 actgaaatgt tttttagaca atcaaatgtt aggtaagtgc aaaaaacttg tttttcttac
    10921 tggtgtagaa attaatgcct tttttatttt tcagttattt tataataaca aaataaaaac
    10981 aacccccagc tgccaggtgg gttttggtgt tcgaaatgcg gggcaaagca ctacatcact
    11041 gcaaatagat acagagttag tctgcatgtc tgtaggctgt gtgattgcgg aaaatataaa
    11101 tgctgctaat atatttcctt tttacagaaa agcatatcta aatagatgat tgttttgatg
    11161 ttaatctttg taaattatgt attaccaatt ttaacattgg atgtaattgc ataaaggctt
    11221 gcatctcaat ccttgaaagt ctagcattaa atggaaacaa cttttcctaa aaaaaaaaaa
    11281 aaaaaaaaaa aaa
//
