LOCUS       NM_001008232            5582 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
            (Asap3), transcript variant 1, mRNA.
ACCESSION   NM_001008232 XM_131741 XM_485459
VERSION     NM_001008232.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Qian,J., Li,Y., Yao,H., Tian,H., Wang,H., Ai,L., Xie,Y., Bao,Y.,
            Liang,L., Hu,Y., Zhang,Y., Wang,J., Li,C., Tang,J., Chen,Y., Xu,J.
            and Fang,J.Y.
  TITLE     ASAP3 regulates microvilli structure in parietal cells and presents
            intervention target for gastric acidity
  JOURNAL   Signal Transduct Target Ther 2, 17003 (2017)
   PUBMED   29263912
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5582)
  AUTHORS   Ha,V.L., Bharti,S., Inoue,H., Vass,W.C., Campa,F., Nie,Z., de
            Gramont,A., Ward,Y. and Randazzo,P.A.
  TITLE     ASAP3 is a focal adhesion-associated Arf GAP that functions in cell
            migration and invasion
  JOURNAL   J Biol Chem 283 (22), 14915-14926 (2008)
   PUBMED   18400762
  REMARK    GeneRIF: ASAP3 functions nonredundantly with ASAP1 to control cell
            movement and may have a role in cancer cell invasion.
REFERENCE   4  (bases 1 to 5582)
  AUTHORS   Garrett-Engele,C.M., Tasch,M.A., Hwang,H.C., Fero,M.L.,
            Perlmutter,R.M., Clurman,B.E. and Roberts,J.M.
  TITLE     A mechanism misregulating p27 in tumors discovered in a functional
            genomic screen
  JOURNAL   PLoS Genet 3 (12), e219 (2007)
   PUBMED   18069898
REFERENCE   5  (bases 1 to 5582)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL935264.12 and BC085250.1.
            
            On Aug 25, 2009 this sequence version replaced NM_001008232.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085250.1, SRR9219382.149115.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AL935264.12        100406-100464
            60-3918             BC085250.1         13-3871
            3919-5582           AL935264.12        138951-140614
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 68.34 cM"
     gene            1..5582
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /note="ArfGAP with SH3 domain, ankyrin repeat and PH
                     domain 3"
                     /db_xref="GeneID:230837"
                     /db_xref="MGI:MGI:2684986"
     exon            1..283
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..2869
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     development and differentiation enhancing factor-like 1;
                     arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 3"
                     /codon_start=1
                     /product="arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 3 isoform 1"
                     /protein_id="NP_001008233.1"
                     /db_xref="CCDS:CCDS18802.1"
                     /db_xref="GeneID:230837"
                     /db_xref="MGI:MGI:2684986"
                     /translation="MPEQLSVAEFLAVTAEDLSSPAGAAAFAAKMPRCRGAALAREEA
                     LEGDQAILQRIKKAVRAIHSSGLGHVETEEHYREAVEALGNSHLSQNSHELSTGFLNL
                     AVFTREVAALFKNLVQNLNNIVSFPLDSLMKGHLRDGRHDSKKHLEKAWKDYESKVAK
                     LEKERDRARFPGGSHGVMSQDTQRERRVFQLHMCEYLVKAGESQVKQGPDFLQSLIKF
                     FHAQHNFFQDGWKAAQSLSPFIDKLAASVHGLRQAQEEELHKLTQLRDSLRGMLHLES
                     REDHPNRKNSGGGYSIHQHQGNKQFGTEKVGFLYKKSDGIRRVWQKRKCGVKYGCLTI
                     SHSMINRPPVKLPLLTCQVRPNPEEKRCFDLVTHNRTYHFHAEDEQECEAWVSVLQNS
                     KDEALSNAFHGEPSGGQWSWGTRLDTEPHDLTNMLVAEVKSRPGNDRCCDCGAADPTW
                     LSTNLGVLTCIQCSGVHRELGVRFSRIQSLTLDLLGPSELLLALNIGNSHFNEVMEAH
                     LPSHGSPKPSAESDMSSRRNYIVAKYVEHKFARHSTPDPQKLRTAICSRDLLSVLEAF
                     ANGQDFGQLLPGPDGQAPGELALHLAIRVASHASLPIVDFLIQNGGHLDAKAADGNTA
                     LHCAALHGQLDCLKLLLRGRAPVGAVNDAGETALDIARNRQHKECEELLEQAQAGTLA
                     FPLHMDYHWGHSMEHGFDSEEEEEEEKHCPSKPPAQACWGSVRLDISNKTYETVATPG
                     PATTQSQSEDSPPPLPIKNSSRTIVLGRAGHCSGDRSDLPSLRSESPEALENRSSPAS
                     SSSSLTSSVEPGGLSQAPSSPEEGLQESASISRPGLASGTTSAEVYLPVKFSSESTRS
                     YRRGGRSLEDSPSARQPLCSRRHIPVGLVEGDGSKIGVLPDSLQLLHD"
     exon            284..356
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            357..502
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            503..577
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..627
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..739
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..825
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..901
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..988
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1098
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1168
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1256
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1305
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1493
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1627
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1722
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1900
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..1986
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2102
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2170
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2281
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2477
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2703
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2797
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2798..5582
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3912
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /note="major polyA site"
     regulatory      4203..4208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /note="hexamer: AGTAAA"
     polyA_site      4222
                     /gene="Asap3"
                     /gene_synonym="9430088F20Rik; Ddefl1; Gm140; UPLC1"
                     /note="major polyA site"
ORIGIN      
        1 agttaggggc ggggcggagc agggagtgaa gaggcctcga ctaggacgct gcgggtggtg
       61 agacaggccc tgcgcagagc agctatacca gcactgttaa gtgcagcgtc ccccgcgttc
      121 caggcagctc cggtgttttc gcctcgttcc caccatgccc gagcagctca gtgtcgcgga
      181 gttcctggcc gtcaccgcag aggacctcag ttccccagcc ggagcagctg ccttcgccgc
      241 caagatgccc cggtgccgcg gggcggcgct ggcacgggag gaggccctgg aaggagacca
      301 ggctatctta cagaggatca agaaggctgt tcgagctatc cacagctctg gtctcggcca
      361 cgtggagact gaggagcatt accgagaggc cgtggaggcc ttggggaata gtcacttgtc
      421 tcaaaacagc cacgagctgt ccactggctt cctgaacctg gctgtattca ccagagaggt
      481 ggctgccctc ttcaagaacc tggttcagaa cctgaacaac atcgtctcct tcccgctgga
      541 cagtctgatg aaggggcatc taagggatgg gcgacatgat tccaaaaagc atctggagaa
      601 ggcgtggaaa gactacgaaa gcaaagtggc caaactggag aaggagaggg atcgggccag
      661 gttcccagga ggaagccatg gggtgatgtc ccaggacaca cagcgagagc gacgagtctt
      721 ccagctgcac atgtgtgagt accttgtcaa agctggagag agtcaggtga agcaaggccc
      781 agacttcctg cagagcctca tcaagttctt ccatgcccag cacaacttct tccaagatgg
      841 ctggaaggca gctcagagcc tgtccccctt catcgacaag ctggcagcct cagtgcatgg
      901 gcttcggcaa gctcaggaag aggaactgca caagctcacc cagctccggg actccctccg
      961 agggatgctg cacctggaga gcagagagga tcacccaaac cggaagaact cagggggcgg
     1021 ctacagcatc caccagcacc aaggcaacaa gcagtttggg actgagaagg tgggcttcct
     1081 gtacaagaaa agtgatggga ttcgaagagt ctggcagaag aggaagtgtg gagtgaagta
     1141 tggctgcctg accatctcgc acagcatgat caacaggccc ccagtgaagc tgcccttgct
     1201 gacgtgccaa gtgaggccca accccgagga gaaaaggtgt ttcgacctgg tgacccacaa
     1261 ccgcacttac cacttccatg cagaggacga gcaggagtgt gaggcatggg tgtcagtgct
     1321 gcagaatagc aaggacgaag ccctgagcaa tgcctttcac ggggagccca gtggcggcca
     1381 gtggtcttgg ggcacccgac tggatacgga gccccacgac ctaaccaaca tgcttgtcgc
     1441 tgaggtgaag agcaggccag ggaatgaccg ttgctgcgac tgtggggctg cagaccccac
     1501 gtggctcagc accaacctgg gcgtgctcac ctgcatccag tgctccggtg tccaccggga
     1561 gctgggcgtg cgcttctccc gcattcaatc gctcaccttg gacctcctgg gcccctcgga
     1621 gctgctgctg gctttgaaca tcggaaactc ccacttcaat gaagtcatgg aggcccacct
     1681 gccctcccac ggcagcccta aaccctctgc tgagagtgac atgagcagcc gcaggaacta
     1741 cattgtagcc aagtatgtag aacacaagtt cgcacgccat tccacaccag atccccagaa
     1801 gctacggaca gccatctgca gccgggacct cctctccgtc ctggaggcct ttgccaatgg
     1861 gcaggacttc ggacagctgc tgccagggcc tgatgggcag gcaccaggag agctcgccct
     1921 gcacttggcc atcagagtcg ccagccatgc ttccctgccg atagtggact tcctcatcca
     1981 gaatggtggc cacttggatg ccaaagctgc agatggaaac accgccctgc actgcgcggc
     2041 gctccacggc cagctagact gtctcaaact gctgctaaga ggaagggctc ccgtgggggc
     2101 agtaaatgat gctggagaga cagccctgga catagccaga aacagacagc acaaggaatg
     2161 tgaagagctg ctggagcagg cacaagcggg gacgcttgct ttccctctgc atatggacta
     2221 ccactgggga cattccatgg aacacggctt tgacagtgag gaggaggagg aggaagaaaa
     2281 gcactgcccc tcgaagcccc cagcgcaggc ctgctggggt agtgtgaggc tggacatcag
     2341 caacaagacc tatgagaccg ttgccacccc gggaccagcc accactcaga gccaaagtga
     2401 ggacagtccc ccacccctgc ccatcaaaaa ctcttctcgg accatagtcc tcgggcgagc
     2461 cggacattgc agtggagatc gttctgacct tcccagcctg agatcagaat ctcctgaggc
     2521 cttagaaaac cggagcagcc cagcatcctc ctcctccagc ctcaccagct cagtggaacc
     2581 cggggggctg agccaggccc cgtctagccc tgaagagggc ctccaggaat cagccagcat
     2641 ctcacgccct ggcctggcat ctggaactac ctctgctgag gtgtacctcc ctgtcaaatt
     2701 cagctcagag agcactcgat cctacagacg gggaggccga agtctggaag acagtccctc
     2761 tgcccggcag cctctgtgta gtagaaggca cattccggtt ggcctcgtgg aaggagatgg
     2821 ctcaaagatc ggggttctcc cggattccct gcaactcttg cacgactagc tccttgctga
     2881 cccctctgca tggctcacac tggaccccaa cattcagagc tgggacttga gaccgcagat
     2941 ctgggagctc acattcctta tgggaaccat gaccctgcca aggacactgg cccttctgtg
     3001 ttggggctga cgtcctgagc caggcagccc tggagttaga tctgacaatg tctgggttga
     3061 gttaagttac acaactctca atacctacta tattaggatt gtgtttctcc ctaccagcgt
     3121 gggacccgtt ctcatcattc atgcccaggt gctgtctcgt ggccacttat caatctggtg
     3181 actttcttta gcgaagtcca gctttatgtc cagacctttg ctgggtacta tggatgccac
     3241 atgaatagga cacacatccc gcaggtgcgg atcctcactg gaaagctggg ctcaagaact
     3301 gactcggaag gagcagagct aggttgcaga gggatggctc tgagctcagg gccgggactg
     3361 acctcagctg gtctcctgtc tggtcactgc agttgttaca gcccagttcc ggtggccaag
     3421 gcctgtgact cccagtctaa agtatgctcc attagagggc agccttaccc tccctggggt
     3481 ctgtactgtg tccctccgtc tggaagtaga atatgactca cctgccttga tttcacagaa
     3541 aggaaaagga tggtctgggt tgacctatct catttctctc acttttctct cgatgaattc
     3601 agaagcctgg gggaacgagg gatcaggaaa ggagaaaggc gctgggaaga taagagaaac
     3661 ttcaaaagtc tgctttctag ggccagaaat cccaccatcg cttagtccaa ttacaaagcc
     3721 agaccaccaa caaccatgga cagctctgtt tcacacctcc ttggtggtgg ggcctgcagg
     3781 ggtgggccaa gccgaacagg ggaggccaga gggcaccaag tgagtagcca gcagtgtggc
     3841 taggaattga actgtgcact ctggaggctg ctgtggtcct tggtcctaaa acaggattgt
     3901 ttctgagccc caggtcaact tgtgttcatc tcattcttac tgttttactt atcaaaggat
     3961 tcctggggga agggacgaat tcccctggga gactcttggg acttgggatg ctttccctgg
     4021 ccaggttggt aaagagtagt ggtcagagaa caacagggag ttttgccaca ggagacattt
     4081 ggtgaggcaa ggactggagg gggcatcgct gagctcaggg tgccctcagc atcctcctta
     4141 cagcatctct taatctgtct tgtgtcgttg taacggaacg atagaggctg ggtgctttct
     4201 acagtaaaga gttttctttg gcttagggtt ctggtgattg gaaagtccaa acagcatgcc
     4261 atgaatgccc tgtaaaaggc cccgtctgtc acaacatcac aaaagaggag aaagcaagca
     4321 cttatgtgca gtaatcgcaa tttgctttat ttatagaatc tcacttctgt aagaactcac
     4381 tccctagagg cagagcccct cacataattg ccttccacta ggcgctgccc cttaaaggct
     4441 cactcaccct tccacattgc cacaccagga aggaagaaaa cacactcaaa acacacccac
     4501 atgagccggg cgtggtggca cacaccttta attgcagcac tcgggaggca gaggcaagca
     4561 gatttttgag ttcgaggcca gcctggtcta cagagtaagt tccaggacag tcagggctac
     4621 acagagaaac cctgtctcac aaaaaaacaa aaaacaaaac aaaaaaaaaa cccataccca
     4681 catggtatgg tttatcctca cagcaatgaa ctagccaatc caaaatgttg tcagcagcca
     4741 ggctcagaac ccctggtgtg caggaaaagc acactgggcc agagcctgag gacctgattc
     4801 gtggctcacc catggtcccg accacacccc ctcttggttg ccctgagatg atgagttggg
     4861 taatggagtt tgtgaggtga gccctgaatg cataacaagg ggagaaatga aacagaaaag
     4921 gtttgtcagc agtgaaggac agagaagaag gctgttggga aagtgcttgc catacaacac
     4981 gcaaggttga ggatttgagt tcgaatctgc agggcttatg ccaagagcta agtacacagt
     5041 gtcctcccag actcctggct ctgtggaagc agaccctgga ggatcccgga agcctgctgg
     5101 gcagctccag gttcagtgag agaccctgtc tcaaaacata aggaggctag caattgagac
     5161 caccaccagt ctctggctgc ctgcctcctg ccgagtacac acacacacac acacacacac
     5221 aagcataaaa gacgctggca cagctgcaca catctgtaat tccagcattc tggggactga
     5281 ggcagggcca tagtgagtta gcaaatacct ttgcgctgtc cctttgagag taaagaatgg
     5341 ggtgtttacc accgatggtg aaacccctct gagggtatca gttccctgtc tgcccccccc
     5401 ccccaaagct cacagggaaa taagacagtt gtgtgcatct ttgtgtgcag gtgacctctc
     5461 tcttggacag gtgggcagaa gggatacaat gggtaccagc aaatgctggc tgtaccgagg
     5521 aggcggcctc ccacttcctg agattgactc ttctatttgc ataataaagt ctgacgcgtt
     5581 tt
//
