LOCUS       NM_001135192            5686 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
            (Asap2), transcript variant 3, mRNA.
ACCESSION   NM_001135192
VERSION     NM_001135192.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Zhao,J., Wang,M., Deng,W., Zhong,D., Jiang,Y., Liao,Y., Chen,B. and
            Zhang,X.
  TITLE     ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT
            phosphorylation in the IR/IRS1/AKT pathway by interacting with
            ASAP2 and regulating PDPK1 activity
  JOURNAL   Biochem Biophys Res Commun 486 (4), 865-871 (2017)
   PUBMED   28322786
  REMARK    GeneRIF: In conclusion, we have identified that ARL15 acts as an
            insulin-sensitizing effector molecule to upregulate the
            phosphorylation of members of the canonical IR/IRS1/PDPK1/AKT
            insulin pathway by interacting with its GAP ASAP2 and activating
            PDPK1. This research may provide new insights into GTPase-mediated
            insulin signalling regulation and facilitate the development of new
            pharmacotherapeutic targets for insulin sensitizati
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Norton,R.L., Fredericks,G.J., Huang,Z., Fay,J.D., Hoffmann,F.W. and
            Hoffmann,P.R.
  TITLE     Selenoprotein K regulation of palmitoylation and calpain cleavage
            of ASAP2 is required for efficient FcgammaR-mediated phagocytosis
  JOURNAL   J Leukoc Biol 101 (2), 439-448 (2017)
   PUBMED   27601625
  REMARK    GeneRIF: these findings identify ASAP2 as a new target of
            Selk-dependent palmitoylation and reveal a new mechanism regulating
            the efficiency of FcgammaR-mediated phagocytosis.
REFERENCE   3  (bases 1 to 5686)
  AUTHORS   Cheerathodi,M., Vincent,J.J. and Ballif,B.A.
  TITLE     Quantitative comparison of CrkL-SH3 binding proteins from embryonic
            murine brain and liver: Implications for developmental signaling
            and the quantification of protein species variants in bottom-up
            proteomics
  JOURNAL   J Proteomics 125, 104-111 (2015)
   PUBMED   25982384
REFERENCE   4  (bases 1 to 5686)
  AUTHORS   Bjarnadottir,M., Misner,D.L., Haverfield-Gross,S., Bruun,S.,
            Helgason,V.G., Stefansson,H., Sigmundsson,A., Firth,D.R.,
            Nielsen,B., Stefansdottir,R., Novak,T.J., Stefansson,K.,
            Gurney,M.E. and Andresson,T.
  TITLE     Neuregulin1 (NRG1) signaling through Fyn modulates NMDA receptor
            phosphorylation: differential synaptic function in NRG1+/-
            knock-outs compared with wild-type mice
  JOURNAL   J Neurosci 27 (17), 4519-4529 (2007)
   PUBMED   17460065
REFERENCE   5  (bases 1 to 5686)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   6  (bases 1 to 5686)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 5686)
  AUTHORS   Uchida,H., Kondo,A., Yoshimura,Y., Mazaki,Y. and Sabe,H.
  TITLE     PAG3/Papalpha/KIAA0400, a GTPase-activating protein for
            ADP-ribosylation factor (ARF), regulates ARF6 in Fcgamma
            receptor-mediated phagocytosis of macrophages
  JOURNAL   J Exp Med 193 (8), 955-966 (2001)
   PUBMED   11304556
REFERENCE   8  (bases 1 to 5686)
  AUTHORS   Mandiyan,V., Andreev,J., Schlessinger,J. and Hubbard,S.R.
  TITLE     Crystal structure of the ARF-GAP domain and ankyrin repeats of
            PYK2-associated protein beta
  JOURNAL   EMBO J 18 (24), 6890-6898 (1999)
   PUBMED   10601011
REFERENCE   9  (bases 1 to 5686)
  AUTHORS   Andreev,J., Simon,J.P., Sabatini,D.D., Kam,J., Plowman,G.,
            Randazzo,P.A. and Schlessinger,J.
  TITLE     Identification of a new Pyk2 target protein with Arf-GAP activity
  JOURNAL   Mol Cell Biol 19 (3), 2338-2350 (1999)
   PUBMED   10022920
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CD351639.1, AK147622.1, AC156032.4 and BC096022.1.
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (c).
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164134,
                              SAMN01164137 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               CD351639.1         1-282
            283-1367            AK147622.1         291-1375
            1368-1368           AC156032.4         42956-42956
            1369-1892           AK147622.1         1377-1900
            1893-1911           AC156032.4         63329-63347
            1912-2339           AK147622.1         1920-2347
            2340-2373           AC156032.4         71371-71404
            2374-3097           AK147622.1         2382-3105
            3098-5651           BC096022.1         2740-5293
            5652-5686           BC096022.1         5295-5329
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 8.29 cM"
     gene            1..5686
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /note="ArfGAP with SH3 domain, ankyrin repeat and PH
                     domain 2"
                     /db_xref="GeneID:211914"
                     /db_xref="MGI:MGI:2685438"
     exon            1..414
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             289..3291
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /note="isoform c is encoded by transcript variant 3;
                     development and differentiation enhancing factor 2;
                     arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 2; pyk2 C-terminus-associated
                     protein; paxillin-associated protein with ARF GAP activity
                     3"
                     /codon_start=1
                     /product="arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 2 isoform c"
                     /protein_id="NP_001128664.1"
                     /db_xref="CCDS:CCDS49037.1"
                     /db_xref="GeneID:211914"
                     /db_xref="MGI:MGI:2685438"
                     /translation="MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEAL
                     DVDRMVLYKMKKSVKAINISGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFS
                     VFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKI
                     EKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKVKKGVDLLQ
                     NLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTAQDEERRQLIQLRDILKSALQV
                     EQKESRRDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGF
                     LTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVL
                     QNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTN
                     LGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPSE
                     DPVKPNPGSDMIARKDYITAKYMERRYARKKHADTAAKLHSLCEAVKTRDIFGLLQAY
                     ADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTA
                     LHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFN
                     SHVHVEYEWRLLHEDLDESDDDVDEKLQPSPNRREDRPVSFYQLGSSQFQSNAVSLAR
                     DTANLTKDKQRGFGPSILQNETYGAILSGSPPSSQSIPPSTTSAPPLPPRNVGKVQTA
                     TSANTLWKTNSVGVDGISRQRSSSDLPAVHPPLPPLRVTSTNPLTTTPPPPVAKTSGT
                     LEAMNQPSKSSQPGTSQSKPPPLPPQPPSRLPQKKPASGTDKPTPLTNKGQPRGPEAS
                     GPLSNAMALQPPAPMPRKSQATKSKPKRVKALYNCVADNPDELTFSEGDVIIVDGEED
                     QEWWIGHIDGEPSRKGAFPVSFVHFIAD"
     exon            415..487
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            488..633
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            634..708
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            709..758
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            759..888
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            889..974
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1050
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1128
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1137
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1241
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1311
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1399
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1448
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1615
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1749
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1844
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2034
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2120
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..2236
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2304
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2418
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2671
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2806
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2972
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3022
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3093
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3216
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3217..5670
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5650..5655
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /note="hexamer: AATAAA"
     polyA_site      5670
                     /gene="Asap2"
                     /gene_synonym="6530401G17Rik; Ddef2; Gm1523; Gm592; PAG3;
                     PAP"
                     /note="major polyA site"
ORIGIN      
        1 gcttggtggc gggttgttcc cgccggctgt gcgcgcccgc gtctgggctc gctcgaagag
       61 gacgcggcgg cggcggctgc ggctccagga gcacagcggg cagagctgcg ggctgcacgc
      121 gcccctgctg ccaccgggcg tcccgcggtg gctcccgcgc cctgcggcgg cttccgcgcc
      181 ccgcgctcct ctttgtccgt gtccctgagc ggctgctgcg gcggggtcgg agcccgcgcc
      241 ctgggcgact gaggcggcgg cggcagtccg cgccccggtg ttgcagcgat gccggaccag
      301 atctccgtgt cggaattcgt ggccgagacc catgaggact acaaggcgcc cacggcctct
      361 agcttcacca cgcgcacggc ccagtgccgg aacaccgtgg cggccatcga ggaggctttg
      421 gacgtggacc ggatggttct ctacaaaatg aagaaatctg tgaaggcaat caatatctct
      481 gggctggcac acgtggagaa cgaggaacag tacacccagg ctctggagaa gttcggtggc
      541 aactgtgtgt gcagagatga tccagactta ggaagcgcgt ttctgaagtt ctctgtgttc
      601 accaaggagt tgacggcact cttcaaaaac ctgattcaga acatgaacaa catcatctcg
      661 ttccctttgg acagtttgct gaaaggagat ctgaaaggag taaaagggga tctgaaaaag
      721 ccctttgata aagcttggaa ggactatgaa acaaaaataa ccaaaataga aaaggagaaa
      781 aaggagcatg ccaagctcca cgggatgatt cgtactgaaa taagcggggc tgagattgca
      841 gaggagatgg agaaggaaag acggttcttc cagctgcaga tgtgtgagta cctgctgaag
      901 gtcaatgaaa tcaaggtgaa aaagggagtg gatttgcttc agaatctgat caagtacttt
      961 cacgcccagt gcaatttttt ccaggatgga ttgaaagcag tagaaagcct caagccttcc
     1021 atcgagacgc tctccacgga cctccacacc gcccaggatg aggaacggcg acagctgata
     1081 caacttcgag atattttgaa gtcagcattg caggtggaac agaaagagtc taggagagac
     1141 tcacaacttc gccaaagtac agcctacagc ttacatcaac ctcagggaaa caaggagcat
     1201 ggaacagaga ggaacgggaa cctctacaag aagagcgatg ggatccggaa agtgtggcag
     1261 aagaggaagt gttccgttaa aaatggcttc ctcaccatct cccacggcac tgccaaccgg
     1321 ccacctgcca agctcaacct gctaacctgc caggtgaaga ccaatcctga ggagaagaag
     1381 tgtttcgacc tcatatcaca tgacaggacg taccacttcc aagcggaaga cgaacaagaa
     1441 tgtcagatat ggatgtctgt actgcagaac agcaaggaag aagctctgaa caacgccttt
     1501 aagggtgatg acaacactgg agaaaataac atcgtccaag agctgaccaa ggagatcatc
     1561 tcggaggtgc agaggatgac gggcaatgac gtgtgctgcg actgtggggc accagatccg
     1621 acgtggctct ctaccaacct gggcatcctg acttgcatcg agtgctctgg gatccaccgg
     1681 gagctggggg ttcattactc caggatgcag tccctgacat tagatgtatt gggaacgtct
     1741 gagcttctgc ttgccaagaa tatcgggaat gcaggcttta atgaaattat ggagtgttgc
     1801 ctaccgtctg aggacccagt caagcccaac ccaggcagtg acatgattgc aaggaaggac
     1861 tacatcacag ccaagtacat ggagaggaga tatgcacgga aaaagcatgc cgacaccgcg
     1921 gcgaagctcc acagcctttg cgaggccgtc aagaccagag acatttttgg gttactccaa
     1981 gcttatgctg atggtgtgga cctgacagag aaaatcccac tggccaatgg gcatgagcca
     2041 gatgagacag ccctccatct tgcagtcaga tctgtggacc ggacttccct tcacattgta
     2101 gacttcctgg tccagaacag tgggaacctg gataaacaga caggcaaggg cagcacagcc
     2161 ctgcactact gctgcctgac agacaatgct gagtgcctca agctcctcct gcggggaaaa
     2221 gcctccattg agatagccaa tgagtcagga gagaccccgt tggacattgc taagcggctc
     2281 aagcacgaac actgtgagga actgctcact caggccttgt cggggcggtt taactcccat
     2341 gttcacgtcg agtatgaatg gcggctgctg cacgaggacc tggatgagag cgacgacgat
     2401 gtggacgaga agctacagcc tagtcccaac cgacgggagg accggcctgt tagcttctac
     2461 cagctggggt ccagccagtt tcagtccaat gctgtgtctt tggccagaga tactgcaaac
     2521 ctcaccaagg acaagcagag gggctttggg cccagcatct tgcagaatga gacctatgga
     2581 gccatcctga gtggcagccc gccctcctcc cagtcaatac cccccagcac caccagcgca
     2641 cccccgctcc cacctcggaa tgttggcaaa gttcagacag ccacctcagc taacaccctg
     2701 tggaagacaa actctgtagg tgtggacggt atcagcaggc agcgatcttc gtcagatctg
     2761 ccagctgtcc acccaccgct gccccctctt cgagtgacat ctaccaatcc cctgaccacc
     2821 acaccacccc ctcctgtggc taagacatca ggaactctag aagccatgaa ccagccgagc
     2881 aagtcctccc aacctgggac ctcacagagc aagcccccgc ccctgccacc tcagccaccc
     2941 agccgcctgc ctcagaagaa accagcttcc gggactgaca agccaacccc gctgaccaac
     3001 aaaggacagc cgagaggacc tgaagcttcg ggtcctctgt ccaacgccat ggccctgcag
     3061 cccccagcac ccatgcctag gaagtcacag gcgaccaagt caaagcccaa gcgagtgaaa
     3121 gcactctaca actgcgtggc tgacaaccca gacgagctga ctttctctga gggcgacgtg
     3181 atcattgtgg atggggaaga ggatcaggag tggtggatcg gccacatcga tggagagccc
     3241 agccgcaaag gagcgtttcc cgtatcgttt gtgcacttta ttgctgacta aactgccact
     3301 gaacaaaagc ctttgtttaa ccgttatgtt ccgttttgtt actggtacca aggctagcca
     3361 tagccagtct cacggactgt gttatacggc agcccatctt ttcatgatac tgtcctgttc
     3421 tgaaaactca gagtcgagtt ttatatataa gcaggttgtt gttttaataa gttgtggttt
     3481 ttacaaaaca ttgttatact tttactagga aaagcaattt ccttaagggg gcggtgagaa
     3541 gctatttaac tgtataccca ggatcctgta tttccaggag tagctgagcc taaaaacagc
     3601 ttaaacaaat ttgggggatt cttagttttc cttagcccaa ttccgacatt ggttatcttg
     3661 tttagcacag tcagcttagc aagccagcac catactgctt ccaggtctgt cgccctaccc
     3721 tggaccttgt ccaccaatgc catccattca cctttaaaga catgtattcc tttgaaactc
     3781 agccagtgtc tatttaaagc aacttggcaa tttatacgtt agccttgtac ttttccccat
     3841 ctgtgaggtg gcagctgcca tgatctcccg catgtttgta ggacccatgt tgtgaacagt
     3901 catacctagc acactactga tgggttatta caacacagct aacagcaggc tgttctacac
     3961 caggtagttt aagctgcaga tcctgaattt ggttaaaaac taaggatgat ggattgcaga
     4021 gcagctcttt aaactagctc gcatgcctgg gggtttgagg atttgcctcg ttcagcatcc
     4081 cgtcacacag gtagaatatg tggtgcgggc cacagtaagc acattcttcc gtgtcctggt
     4141 gcgtgctcct gccctcccct cccagctcct gaggacaccc gctcagctta gttcttgtct
     4201 taataggatt ctccaaaagg aatggtagct acactgtctt gaaacttagc ctatccaatt
     4261 gtttgtagtc aagaacatat cagaaatctg tagaccttgg ggagcccagt gcccccaaac
     4321 atcccagtta cactatgtaa gcccatcgca tcactcctaa acttacacta tgtggggtgg
     4381 gggaggggaa ctaggttgta tatgataaaa atcaaaactc attagtttca tgaacttgac
     4441 tgtcatacct ctacttaata acattaagca caggtaagag ccgtggttag gatatgggaa
     4501 atgaaagagg caagcataag gcctgaacga aagctggagt ttgagcaaaa ggaatggatt
     4561 gagggttaag cttgacagtg aggcgatgtt gctgccggaa gtggcacact tgatcgaagc
     4621 agccccttct ttatgctctt tgtgcaaggc tcttacaagc tcagagctct ctctttgttt
     4681 ctttcaagga aaacaggaca aactggaatg tcttaggatg ctatatagtg attgcttcct
     4741 gtctttcaga ttctgggtga aaatgtgtta gatctgtaat tgatctctct ctctctctct
     4801 ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct ctgctccccc
     4861 aacactacat ctgtttttca ctacttgtta atttctgtcc ctttagccct tctcatcact
     4921 tttctcgtag acttaagagc gtgaacagat gcgttttgca cattgcactt cccaatacct
     4981 tttacagtgc ctcccccacc cccaccccca cccccacacg catctgtatc atcggtctta
     5041 ggtacctggt gccagctctc tgaggaatct agttgattta tgtcaacatt ttaactccag
     5101 tcccaagcta atctatcagg ttcactggta cataaatacc taggaaatat ttttccgctc
     5161 tctaatttgg tggcactatt gtgcagtaat gggtagtcct ttttaccaga agagaagttc
     5221 tattgacttc cctccttaat tgtccttcct gttactcgct ctttgcaaac tgagaagaga
     5281 aagcaactga aaggaaagca ttgtagatat ctatttatat ttaaagttta catttcacaa
     5341 gcgtcagctg cggaatcgga ctttttttcc gctgctttcc atcaggtctg acttggggga
     5401 cgatgactga gaaatagtgc gcagactaat gtgatctgct gaggtggaga atgacctgca
     5461 gccaagaatg ctattttgag atgtatgata ttcaattcat tcacttgatt actttggtct
     5521 agttgcagcg caactgtata tactgtataa cctaaacgga ttattttcat tgtcttaatg
     5581 cagtgattta taattagagc atgtttaata aattgacttc ctgttaacca gtcatttgac
     5641 tggaaaaaaa ataaagtact tttaaatgga aaaaaaaaaa aaaaaa
//
