LOCUS       NM_010026               6151 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1
            (Asap1), transcript variant 1, mRNA.
ACCESSION   NM_010026 XM_906698 XM_919877 XM_919886 XM_919902 XM_919913
            XM_919921 XM_919929 XM_919942 XM_919954 XM_919967 XM_919977
            XM_919987 XM_919999
VERSION     NM_010026.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Zhang,Y., Bai,H., Zhang,W., Gao,J., Gao,C., Deng,T., Liu,X.,
            Sun,X., Liu,Y., Wang,N. and Wu,Y.
  TITLE     miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1
  JOURNAL   Exp Cell Res 433 (2), 113828 (2023)
   PUBMED   37875175
  REMARK    GeneRIF: miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1.
REFERENCE   3  (bases 1 to 6151)
  AUTHORS   Schreiber,C., Gruber,A., Rosswag,S., Saraswati,S., Harkins,S.,
            Thiele,W., Foroushani,Z.H., Munding,N., Schmaus,A., Rothley,M.,
            Dimmler,A., Tanaka,M., Garvalov,B.K. and Sleeman,J.P.
  TITLE     Loss of ASAP1 in the MMTV-PyMT model of luminal breast cancer
            activates AKT, accelerates tumorigenesis, and promotes metastasis
  JOURNAL   Cancer Lett 533, 215600 (2022)
   PUBMED   35181478
REFERENCE   4  (bases 1 to 6151)
  AUTHORS   Carbonell,A.U., Cho,C.H., Tindi,J.O., Counts,P.A., Bates,J.C.,
            Erdjument-Bromage,H., Cvejic,S., Iaboni,A., Kvint,I., Rosensaft,J.,
            Banne,E., Anagnostou,E., Neubert,T.A., Scherer,S.W., Molholm,S. and
            Jordan,B.A.
  TITLE     Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a
            neurodevelopmental syndrome
  JOURNAL   Nat Commun 10 (1), 3529 (2019)
   PUBMED   31388001
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6151)
  AUTHORS   Schreiber,C., Saraswati,S., Harkins,S., Gruber,A., Cremers,N.,
            Thiele,W., Rothley,M., Plaumann,D., Korn,C., Armant,O.,
            Augustin,H.G. and Sleeman,J.P.
  TITLE     Loss of ASAP1 in mice impairs adipogenic and osteogenic
            differentiation of mesenchymal progenitor cells through
            dysregulation of FAK/Src and AKT signaling
  JOURNAL   PLoS Genet 15 (6), e1008216 (2019)
   PUBMED   31246957
  REMARK    GeneRIF: Together these observations suggest that ASAP1 plays a
            decisive role during the differentiation of mesenchymal progenitor
            cells
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6151)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 6151)
  AUTHORS   Kam,J.L., Miura,K., Jackson,T.R., Gruschus,J., Roller,P.,
            Stauffer,S., Clark,J., Aneja,R. and Randazzo,P.A.
  TITLE     Phosphoinositide-dependent activation of the ADP-ribosylation
            factor GTPase-activating protein ASAP1. Evidence for the pleckstrin
            homology domain functioning as an allosteric site
  JOURNAL   J Biol Chem 275 (13), 9653-9663 (2000)
   PUBMED   10734117
REFERENCE   8  (bases 1 to 6151)
  AUTHORS   King,F.J., Hu,E., Harris,D.F., Sarraf,P., Spiegelman,B.M. and
            Roberts,T.M.
  TITLE     DEF-1, a novel Src SH3 binding protein that promotes adipogenesis
            in fibroblastic cell lines
  JOURNAL   Mol Cell Biol 19 (3), 2330-2337 (1999)
   PUBMED   10022919
REFERENCE   9  (bases 1 to 6151)
  AUTHORS   Brown,M.T., Andrade,J., Radhakrishna,H., Donaldson,J.G.,
            Cooper,J.A. and Randazzo,P.A.
  TITLE     ASAP1, a phospholipid-dependent arf GTPase-activating protein that
            associates with and is phosphorylated by Src
  JOURNAL   Mol Cell Biol 18 (12), 7038-7051 (1998)
   PUBMED   9819391
REFERENCE   10 (bases 1 to 6151)
  AUTHORS   Yamabhai,M. and Kay,B.K.
  TITLE     Examining the specificity of Src homology 3 domain--ligand
            interactions with alkaline phosphatase fusion proteins
  JOURNAL   Anal Biochem 247 (1), 143-151 (1997)
   PUBMED   9126384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC098728.3, AC131710.3 and AC156573.3.
            
            On Aug 23, 2022 this sequence version replaced NM_010026.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094581.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AC098728.3         179034-179121
            89-174              AC098728.3         211925-212010
            175-301             AC131710.3         204220-204346       c
            302-374             AC131710.3         100355-100427       c
            375-520             AC131710.3         83355-83500         c
            521-595             AC156573.3         189330-189404       c
            596-645             AC156573.3         188797-188846       c
            646-775             AC156573.3         183812-183941       c
            776-861             AC156573.3         182572-182657       c
            862-937             AC156573.3         176334-176409       c
            938-1060            AC156573.3         174994-175116       c
            1061-1069           AC156573.3         169956-169964       c
            1070-1170           AC156573.3         168942-169042       c
            1171-1240           AC156573.3         165718-165787       c
            1241-1328           AC156573.3         154066-154153       c
            1329-1377           AC156573.3         151918-151966       c
            1378-1541           AC156573.3         146216-146379       c
            1542-1675           AC156573.3         145283-145416       c
            1676-1767           AC156573.3         143484-143575       c
            1768-1954           AC156573.3         140830-141016       c
            1955-2040           AC156573.3         140427-140512       c
            2041-2156           AC156573.3         139977-140092       c
            2157-2224           AC156573.3         139784-139851       c
            2225-2332           AC156573.3         138120-138227       c
            2333-2561           AC156573.3         136070-136298       c
            2562-2732           AC156573.3         127018-127188       c
            2733-2802           AC156573.3         118515-118584       c
            2803-2861           AC156573.3         115644-115702       c
            2862-3361           AC156573.3         110121-110620       c
            3362-3484           AC156573.3         108160-108282       c
            3485-6151           AC156573.3         102967-105633       c
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 28.55 cM"
     gene            1..6151
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /note="ArfGAP with SH3 domain, ankyrin repeat and PH
                     domain1"
                     /db_xref="GeneID:13196"
                     /db_xref="MGI:MGI:1342335"
     exon            1..88
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            89..174
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..3559
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /note="isoform a is encoded by transcript variant 1;
                     arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 1; PIP2-dependent ARF1 GAP; 130
                     kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1
                     GTPase-activating protein; ARF GTPase-activating protein
                     1; development and differentiation-enhancing factor 1;
                     ADP-ribosylation factor-directed GTPase-activating protein
                     1; 130 kDa phosphatidylinositol 4,5-biphosphate-dependent
                     ARF1 GTPase-activating protein"
                     /codon_start=1
                     /product="arf-GAP with SH3 domain, ANK repeat and PH
                     domain-containing protein 1 isoform a"
                     /protein_id="NP_034156.2"
                     /db_xref="CCDS:CCDS56986.1"
                     /db_xref="GeneID:13196"
                     /db_xref="MGI:MGI:1342335"
                     /translation="MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTS
                     SFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKF
                     GSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKG
                     DLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQM
                     CEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIK
                     QTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSSRRDSQSRQGGYSMHQLQG
                     NKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKP
                     NAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLE
                     DLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQS
                     LELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHR
                     FSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAV
                     RTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVN
                     QNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKP
                     SPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLP
                     TSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDP
                     PSPLPHGPPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQ
                     KVALRKTETSHHLSLDRTNIPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGE
                     LPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQAGDVSAKVQ
                     PPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVR
                     RVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILS
                     D"
     exon            175..301
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            302..374
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            375..520
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            521..595
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            596..645
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            646..775
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            776..861
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            862..937
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1060
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1069
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1170
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1240
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1328
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1377
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1541
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1675
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1767
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1954
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2040
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2156
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2224
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2332
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2561
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2732
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2802
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2861
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            2862..3361
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            3362..3484
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     exon            3485..6151
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6125..6130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /note="hexamer: AATAAA"
     polyA_site      6151
                     /gene="Asap1"
                     /gene_synonym="Ddef1; DEF-1; mKIAA1249; PAP; s19"
                     /note="major polyA site"
ORIGIN      
        1 cccttcctgc gccgcgctcg gctcccgcgc ctcgcccggc ctcgccgcgc ccgcgatgga
       61 ataatgccca gcggcccgcc cggtcccggt cgttttcgga tgtgacggct gagacatgag
      121 atcttcagcc tcccggctct ccagtttttc ctccagagat tcactatgga atcggatgcc
      181 ggaccagatc tccgtgtccg agttcatcgc cgagaccacc gaggactaca actcgcccac
      241 cacgtccagc ttcaccacgc ggctgcacaa ctgcaggaac accgtcacgc tgctggagga
      301 ggctctagac caagatagaa cagccctaca gaaagtgaag aaatctgtaa aagcaatata
      361 taattccggt caagatcatg tacaaaatga agaaaactat gcacaagttc tggataagtt
      421 tggaagtaat tttttaagca gagacaaccc tgaccttggc accgcttttg tcaagttttc
      481 tacactcaca aaggaactgt ccacgctgct gaaaaatctg ctccagggtc tgagccacaa
      541 tgtgatcttc accttggatt ccttgttaaa aggagacttg aagggggtca aaggagatct
      601 caagaagcca tttgacaaag cctggaaaga ttatgagacg aagtttacaa aaattgagaa
      661 ggagaaaaga gagcacgcaa aacagcacgg gatgatccga acagagataa caggcgctga
      721 gattgcagaa gaaatggaaa aggaacggcg gctttttcag ctccagatgt gtgaatatct
      781 cattaaagta aatgaaatca agaccaaaaa gggtgtggac ctgctgcaga accttataaa
      841 gtattaccat gcacagtgca atttctttca ggatggcttg aaaacggctg ataaactgaa
      901 acagtacata gaaaagctgg ctgctgactt atataatata aagcaaaccc aagatgaaga
      961 aaaaaaacag ctaactgcac tccgagatct aataaaatcc tctctgcaac ttgatccgaa
     1021 agaagtaggt ggtttatatg ttgccagcag ggctaacagt tctagaagag actctcagag
     1081 ccggcaaggt gggtacagca tgcatcagct ccagggcaac aaggagtacg gcagtgaaaa
     1141 gaagggcttc ctgctgaaga agagtgatgg gatccggaaa gtgtggcaga gacggaagtg
     1201 tgcggtcaag aacgggatcc tgaccatctc ccacgcaact tccaacaggc agcctgctaa
     1261 gttaaacctc ctcacctgcc aagtgaaacc gaatgctgag gacaagaagt cttttgacct
     1321 gatatcacat aatcgaacat atcactttca agcagaagat gagcaggatt atatagcgtg
     1381 gatatcagta ctgacgaata gcaaagaaga ggccctaacc atggccttcc gtggtgagca
     1441 gagcacaggg gagaatagcc tggaggacct taccaaagcc atcatcgagg atgtacagcg
     1501 gctccctggg aatgacatct gctgtgactg cggctcatca gaacccacgt ggctttcaac
     1561 caacttgggt attttgacct gtatagaatg ttccggaatc catagggaaa tgggggttca
     1621 tatttctcgc attcagtctt tggaactaga caaattagga acttccgaac tcttgctggc
     1681 caagaatgta ggaaacaata gttttaatga tattatggaa gcaaatttac ccagcccttc
     1741 accaaagccc accccttcaa gtgatatgac cgtgcgtaaa gagtacatca ctgcaaagta
     1801 tgtcgaccac cgcttctcca ggaagacctg tgcctcttcc tcagcaaagc tgaacgaatt
     1861 gcttgaggcc atcaagtcca gggacttact tgcattaatc caagtctatg cagagggggt
     1921 ggagttaatg gagccactgc tggaacctgg gcaggagctc ggggagacag cccttcatct
     1981 tgccgtcagg accgcagacc agacatctct ccatctggtt gatttccttg tacaaaactg
     2041 tgggaacctg gataagcaaa cgtctgtggg gaatacagtt ctgcactatt gcagcatgta
     2101 cggcaagccg gagtgtctga agctgctgct taggagcaag cccaccgtgg acatcgttaa
     2161 ccagaatgga gaaactgcct tggatatagc aaagagactc aaagctaccc agtgtgaaga
     2221 tctgctttcc caggctaaat ctggaaagtt caaccctcat gtccacgtag agtatgagtg
     2281 gaatcttcga caggacgaga tggatgagag cgatgacgat ctggatgaca agccaagccc
     2341 gatcaaaaag gaacgctcgc ccagaccaca gagcttctgc cactcctcca gcatctcgcc
     2401 acaggacaag ctggcactgc cagggttcag cactccacgg gacaagcagc ggctctccta
     2461 cggagccttc accaaccaga tcttcgcctc tacgagcaca gatttgccca catcacccac
     2521 cagtgaggct ccccctttgc cacctcggaa cgccgggaaa ggtccaactg gcccaccttc
     2581 aacactccct ctaggcaccc agacctctag tggcagctcc accctatcca agaagaggcc
     2641 tcctccccca ccaccaggac acaagagaac cctgtctgac cctcccagcc cactacctca
     2701 cgggccccca aacaaaggcg caattccttg gggtaatgat gtgggcccat tatcttcaag
     2761 taagacggcc aacaagtttg aggggctgtc tcagcaagca agcaccagtt ctgctaagac
     2821 tgcccttggc ccgagagtgc ttcctaaact acctcagaaa gtggcactaa ggaagacgga
     2881 gaccagccat catctctccc tcgacagaac caacatccca cctgagactt ttcagaaatc
     2941 atcacagttg acagagttac cccaaaagcc accacttgga gagctgcccc cgaagcctgt
     3001 ggaactggcc cccaagcccc aagttggaga gctgccacct aagcctggag agctaccccc
     3061 taagccccaa ttaggtgacc tgccccccaa gccacagctc tcagacttac ctcccaagcc
     3121 acagatgaag gacctgcccc ctaagccgca gctgggggat ctgctggcaa agtcccaggc
     3181 tggcgatgtc tcagccaagg tgcagccacc ctcagaggtc acacagaggt cacacaccgg
     3241 ggatctgtct ccaaatgtac agtccagaga tgccatccag aagcaagcat ctgaagactc
     3301 caacgacctc acacccacgc tgccagagac acccgtacca ctgccgagaa aaatcaatac
     3361 ggggaaaaat aaagtgaggc gggtgaagac catttatgac tgccaggcag ataatgacga
     3421 cgaactcaca tttattgagg gggaagtgat cattgtcacc ggggaagagg accaggagtg
     3481 gtggatcggg catatcgaag gacagcctga aaggaagggt gtctttccag tgtcctttgt
     3541 ccacatcctg tctgactagc aaaaaagcag aaccttctgt ccgtccgtcc acacccttca
     3601 tgccagaccg ctgtctccat gtaatcctgt gtacaacatg tatatagctg ctgttacaga
     3661 ccaagaactc atggaagggc cactcaggag gggaatatag tatgtattgt aaatatcctg
     3721 tggtcttctg ccttcaccag tattagggta acctcgggac ctggtgcgcc ttaccggttt
     3781 gccaaagcca tccttggcat ctagcactta catctctctg tgctgtttta tgagcaaaca
     3841 aaaaccagga gtataggaac tgctggcttt ccaggtgagg agtctccagc agccgtcgaa
     3901 gggtgcagta ccgactccta acaatgcagt attctcaatg gcgttactga acgtgcgaca
     3961 tgagcagagg tgggttctgc gctcccggtg gaacgtaaag ttagtccctg gccagccagc
     4021 ctgtagttat ctgtgtacac agtttacagc tacaaaaacc tactttggta tttattacag
     4081 agaagtgttc agttactcgt gttattcctt cagcaaatac tcactgacac aaaaccctct
     4141 gcagcatttt ccagcatatg cagccttgtg caagtttaga tgagtgggtt tgtacatctt
     4201 atggcgagtg gccacccctg agatattacc tcattatgca gaagtaacat atccttcatg
     4261 actattttga caaaagctta aaaccatgtc tgatgaagtc caactttcag gaaccaagga
     4321 ctgccagaaa ctctctgcct ctacgttatg catgcatcta gacgcttacc cgaattctgc
     4381 cttttataaa ggaacaaaca ggatgaagaa gtgaactgta cagttttaaa cttgattgcc
     4441 attaaagcag aagttacaag attgcaacaa tgcttgtttc tggtcactca tttggccttt
     4501 tccacagtgt ttgcacatgg tgtggacgca gcacccccag cggtgttgaa aagcgtctaa
     4561 ggggtcagtt cgacatgatt ttcttcactt tgttcttgat taagccttga ctattcaagt
     4621 tggaaaactt gtaaagtcgg tctttccgtt ttctcgtgca gaccaggtgc tgatctcacg
     4681 cttttctccc tcctcttctc ttccttaaac tccaatgctg gagtaatttc ataccctcaa
     4741 cttagccagt aaacacgtgc ccttcaggtc agtttataac cgagctgtgg gacgctgcta
     4801 tgaattgcac tgtgataaag ctctctgcaa gccatctctg tctgtctgtc tgtctctgtt
     4861 gttgcttgtg ttttgattct tttccctctg cccattcagc acattgcatc tccacctttg
     4921 gcttcagagt gagtttgggg aaaggatgca gtagctgttt tactcctggg tccaggactg
     4981 ttgtgattct gaggtgtgct gtcatgccat gtcacatttg aggtgtgctg tcatgccatg
     5041 tcacatttgg ttaagttcat tttgagcaca gcacagaaga aaatactctt aaacagataa
     5101 tcttggcaat gaataaaaaa cgcactgatt tcacagaagc agagttcccc ccaaagcttg
     5161 tgggaagggc cacggtgctc agtaaacact cagttctgcc tagcggagac agtgagaact
     5221 ctgaatagcc acccaagaaa taaccttgca ggacaaacct gcaaaatgct ccatcagctg
     5281 ctactgggaa ccgatcgtga gcaattgctt ccgtagtgtc tggacatccg ggtacccggg
     5341 catcaaatcc aggagccaaa ggtgcctgac agcacgctgg tgtaaggccg gcagcacccg
     5401 gcagccatgg tttagaggcc gtaatggctt ctggaatgtg gtagtgatca ttttgttcac
     5461 acgttcttaa gatgcatcga agttgcattt tggaggtcta ggctgtcccc tgctgatttg
     5521 aacataagct gttgttttag ataagcaagt tccatctcta aaatgacagg gagtcgttgt
     5581 tctcttctgc tgcctgcagg gactcacaca aatgtatgtt catctctttc ttttctggtt
     5641 ttttgtttgt ttcccatcag gaatttgagt ttccttcact ccatcgtact gtgggacata
     5701 ggaaaactag gtagacattc ctttggcaat cttgttaagt ttaaatctga ccttgttctg
     5761 gaacggggta agccactatc tacagttttg tacatgacat aattattttg aacgtatgca
     5821 accttaagaa agaaagatca aattcattcc tttttttccc cccaagaagg gaaaaattat
     5881 gtctgcaaac agtgtgtctt attttcctgt tggacagcaa ttgttattta tttttttatt
     5941 gcctactact tggacatggt atctaggagc ccagtcatgc ctgccactag ttaaatccca
     6001 cgttcatacc tgggagttac catgaagcat cagtacactt tttatttcac atgtatttaa
     6061 tgtgacagtt tctcagtact gtatgtgtta atttctactt ttttaatatt taaaattgct
     6121 tttaaataaa catattctca gttgatccca a
//
