LOCUS       NM_018830               4835 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus N-acylsphingosine amidohydrolase 2 (Asah2), mRNA.
ACCESSION   NM_018830
VERSION     NM_018830.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Sun,R., Lei,C., Xu,Z., Gu,X., Huang,L., Chen,L., Tan,Y., Peng,M.,
            Yaddanapudi,K., Siskind,L., Kong,M., Mitchell,R., Yan,J. and
            Deng,Z.
  TITLE     Neutral ceramidase regulates breast cancer progression by metabolic
            programming of TREM2-associated macrophages
  JOURNAL   Nat Commun 15 (1), 966 (2024)
   PUBMED   38302493
  REMARK    GeneRIF: Neutral ceramidase regulates breast cancer progression by
            metabolic programming of TREM2-associated macrophages.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Price,T.R., Emfinger,C.H., Schueler,K.L., King,S., Nicholson,R.,
            Beck,T., Yandell,B.S., Summers,S.A., Holland,W.L., Krauss,R.M.,
            Keller,M.P. and Attie,A.D.
  TITLE     Identification of genetic drivers of plasma lipoprotein size in the
            Diversity Outbred mouse population
  JOURNAL   J Lipid Res 64 (12), 100471 (2023)
   PUBMED   37944753
REFERENCE   3  (bases 1 to 4835)
  AUTHORS   Sun,R., Gu,X., Lei,C., Chen,L., Chu,S., Xu,G., Doll,M.A., Tan,Y.,
            Feng,W., Siskind,L., McClain,C.J. and Deng,Z.
  TITLE     Neutral ceramidase-dependent regulation of macrophage metabolism
            directs intestinal immune homeostasis and controls enteric
            infection
  JOURNAL   Cell Rep 38 (13), 110560 (2022)
   PUBMED   35354041
  REMARK    GeneRIF: Neutral ceramidase-dependent regulation of macrophage
            metabolism directs intestinal immune homeostasis and controls
            enteric infection.
REFERENCE   4  (bases 1 to 4835)
  AUTHORS   Sears,S.M., Dupre,T.V., Shah,P.P., Davis,D.L., Doll,M.A.,
            Sharp,C.N., Vega,A.A., Megyesi,J., Beverly,L.J., Snider,A.J.,
            Obeid,L.M., Hannun,Y.A. and Siskind,L.J.
  TITLE     Neutral ceramidase deficiency protects against cisplatin-induced
            acute kidney injury
  JOURNAL   J Lipid Res 63 (3), 100179 (2022)
   PUBMED   35151662
REFERENCE   5  (bases 1 to 4835)
  AUTHORS   Zhu,H., Klement,J.D., Lu,C., Redd,P.S., Yang,D., Smith,A.D.,
            Poschel,D.B., Zou,J., Liu,D., Wang,P.G., Ostrov,D., Coant,N.,
            Hannun,Y.A., Colby,A.H., Grinstaff,M.W. and Liu,K.
  TITLE     Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived
            Suppressor Cells from Ferroptosis
  JOURNAL   J Immunol 206 (6), 1395-1404 (2021)
   PUBMED   33547170
  REMARK    GeneRIF: Asah2 Represses the p53-Hmox1 Axis to Protect
            Myeloid-Derived Suppressor Cells from Ferroptosis.
REFERENCE   6  (bases 1 to 4835)
  AUTHORS   Okino,N., Mori,K. and Ito,M.
  TITLE     Genomic structure and promoter analysis of the mouse neutral
            ceramidase gene
  JOURNAL   Biochem Biophys Res Commun 299 (1), 160-166 (2002)
   PUBMED   12435403
  REMARK    GeneRIF: molecular cloning of the mouse neutral ceramidase gene and
            its promoter analysis
REFERENCE   7  (bases 1 to 4835)
  AUTHORS   Romiti,E., Meacci,E., Tanzi,G., Becciolini,L., Mitsutake,S.,
            Farnararo,M., Ito,M. and Bruni,P.
  TITLE     Localization of neutral ceramidase in caveolin-enriched light
            membranes of murine endothelial cells
  JOURNAL   FEBS Lett 506 (2), 163-168 (2001)
   PUBMED   11591392
REFERENCE   8  (bases 1 to 4835)
  AUTHORS   Mitsutake,S., Tani,M., Okino,N., Mori,K., Ichinose,S., Omori,A.,
            Iida,H., Nakamura,T. and Ito,M.
  TITLE     Purification, characterization, molecular cloning, and subcellular
            distribution of neutral ceramidase of rat kidney
  JOURNAL   J Biol Chem 276 (28), 26249-26259 (2001)
   PUBMED   11328816
REFERENCE   9  (bases 1 to 4835)
  AUTHORS   Tani,M., Okino,N., Mori,K., Tanigawa,T., Izu,H. and Ito,M.
  TITLE     Molecular cloning of the full-length cDNA encoding mouse neutral
            ceramidase. A novel but highly conserved gene family of
            neutral/alkaline ceramidases
  JOURNAL   J Biol Chem 275 (15), 11229-11234 (2000)
   PUBMED   10753931
REFERENCE   10 (bases 1 to 4835)
  AUTHORS   Tani,M., Okino,N., Mitsutake,S., Tanigawa,T., Izu,H. and Ito,M.
  TITLE     Purification and characterization of a neutral ceramidase from
            mouse liver. A single protein catalyzes the reversible reaction in
            which ceramide is both hydrolyzed and synthesized
  JOURNAL   J Biol Chem 275 (5), 3462-3468 (2000)
   PUBMED   10652340
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB037181.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB037181.1, BC137898.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 26.58 cM"
     gene            1..4835
                     /gene="Asah2"
                     /note="N-acylsphingosine amidohydrolase 2"
                     /db_xref="GeneID:54447"
                     /db_xref="MGI:MGI:1859310"
     exon            1..122
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            123..200
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            201..316
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            317..438
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            439..567
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     CDS             441..2711
                     /gene="Asah2"
                     /EC_number="3.5.1.23"
                     /note="NCDase; N-CDase; acylsphingosine deacylase 2;
                     neutral/alkaline ceramidase"
                     /codon_start=1
                     /product="neutral ceramidase"
                     /protein_id="NP_061300.1"
                     /db_xref="CCDS:CCDS29749.1"
                     /db_xref="GeneID:54447"
                     /db_xref="MGI:MGI:1859310"
                     /translation="MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDS
                     GNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKN
                     GQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYR
                     RDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGK
                     IFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWF
                     AIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNIL
                     GPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASAS
                     QEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTT
                     EGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSL
                     AIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQR
                     YEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADR
                     APIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADW
                     QIMYNDASWETRFYWHKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAV
                     ILAFEGISSPFEVVTT"
     exon            568..728
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            729..878
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1055
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1183
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1261
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1382
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1508
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1595
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1700
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1782
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1898
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..1993
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2033
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2129
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2225
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2372
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2432
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2521
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..4823
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2755..2760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asah2"
                     /note="hexamer: ATTATA"
     polyA_site      2777
                     /gene="Asah2"
                     /note="major polyA site"
     regulatory      3128..3133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asah2"
                     /note="hexamer: AATATA"
     polyA_site      3158
                     /gene="Asah2"
                     /note="major polyA site"
     regulatory      3362..3367
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asah2"
                     /note="hexamer: AATATA"
     polyA_site      3387
                     /gene="Asah2"
     regulatory      4382..4387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Asah2"
                     /note="hexamer: AATAAA"
     polyA_site      4404
                     /gene="Asah2"
                     /note="major polyA site"
ORIGIN      
        1 cctgcagcgg tgttctgaag agccgggcag aggatacaca agcatcccag caggcactct
       61 ggtttgcccg tgaacgatag atatgcgggg gtttgaatga gcagctgcag cagcgggttt
      121 gggtctgtac acatgagtgc tggtgtgctt agaggcatcg ggtccctttc agctggagtt
      181 gcagtacttg tgagtgccat atttggaaac acattgagaa atcagcctga gcaacctgca
      241 aggcacaagg cacaagattc tgcatggtta tttgctctcc caggaggtga acacttgttt
      301 tgattaacag agtcagggtt gagatgccca gttgttcctc atcttggctc agaagaagca
      361 cctaggaata aaagctctaa gctggtatta agtagaatgg gcttaaagtc cactacagga
      421 aacaacagct agtgacagaa atggcaaagc gaaccttctc caccttggag gcattcctca
      481 ttttccttct ggtaataatg acagtcatca cagtggccct tctcaccctc ttgtttgtta
      541 ccagtgggac cattgaaaac cacaaagatt caggaaatca ctggttttca accactctgg
      601 gctccacgac aacccagccc cctccaatta cacagactcc aaacttccct tcatttcgga
      661 acttcagtgg ctactacatt ggcgttggga gagcagattg cacaggacaa gtgtcagata
      721 tcaatttgat gggctatggc aaaaatggcc agaatgcacg gggtctcctc accaggctgt
      781 tcagccgtgc ttttatcttg gcggatccag atgggtcaaa tcgaatggca tttgtgagcg
      841 tggaactatg tatgatttcc caacgactga ggttggaggt cctgaagaga ctagagagta
      901 aatatggctc tctgtatcga agagacaatg ttatcctgag tgccattcac acacactctg
      961 gcccagcagg gtttttccaa tatacactct atatactcgc cagcgaggga ttcagcaacc
     1021 ggacctttca gtacatagtc tctgggatca tgaagagcat tgatatagct cacacaaatc
     1081 ttaaaccagg caaaatcttt atcaacaaag gaaatgttgc taatgtgcag atcaaccgaa
     1141 gcccctcctc ttaccttctg aatccacagt cagagagagc aaggtattct tcaaacacag
     1201 acaaggaaat gctggtcttg aaactggtgg atttgaatgg agaagacttg ggtcttatca
     1261 gctggtttgc catccacccc gtgagcatga acaatagcaa ccactttgtg aatagtgaca
     1321 atatgggcta tgcggcttac ctttttgagc aagaaaagaa caaaggctat ctgcctggac
     1381 agggaccgtt tgtagcaggc tttgcttcat caaatctcgg agacgtgtca cccaacattc
     1441 ttggcccgca ttgtgtcaac acaggggagt cttgtgacaa cgacaagagc acctgtccca
     1501 acggtgggcc tagcatgtgc atggccagcg gacctggaca agacatgttt gagagcacac
     1561 acattatagg acggatcatc tatcagaagg ccaaggagct gtatgcctct gcctcccagg
     1621 aggtgaccgg cccagtgctt gcagctcacc agtgggtgaa catgacagat gtgagcgtcc
     1681 agctcaatgc cacacacaca gtgaagacgt gtaaacctgc cctgggctac agttttgccg
     1741 caggcacaat tgatggagtt tcgggcctca atattacaca gggaactacg gaaggggatc
     1801 cattctggga cactcttcgg gaccagctct tgggaaaacc atctgaagag attgtagagt
     1861 gtcagaaacc caaaccaatc ctgcttcaca gtggagagct gacgatacca catccttggc
     1921 aaccagatat tgttgatgtt cagattgtta ccgttgggtc cttggccata gctgctatcc
     1981 ctggggaatt aacaaccatg tcgggacgaa gatttcgtga ggcaattaaa aaagaatttg
     2041 cactttatgg gatgaaggat atgaccgttg ttatcgcagg tctaagcaat gtttatacac
     2101 attacattac cacatatgaa gaataccagg ctcagcggta cgaggcagca tctacaatct
     2161 atggaccaca caccctgtct gcatacatcc aactcttcag agaccttgct aaggcaattg
     2221 ctacggacac agtagccaac atgagcagtg gtcccgagcc tccattcttc aaaaatctaa
     2281 tagcttcact tattcctaat attgcggata gagcaccaat tggcaaacat tttggggatg
     2341 tcttgcagcc agcaaaacct gaatacagag tgggagaagt ggttgaagtt atatttgtag
     2401 gcgctaaccc aaagaattca gcagagaacc agacccatca aaccttcctc actgtggaga
     2461 aatacgagga ctctgtagct gactggcaga taatgtataa cgatgcctcc tgggagacga
     2521 ggttttattg gcacaaagga atactgggtc tgagcaatgc aacaatatac tggcatattc
     2581 cagatactgc ctaccctgga atctacagaa taagatattt tggacacaat cggaagcagg
     2641 aacttctgaa acccgctgtc atactagcat ttgaaggaat ttcttctcct tttgaagttg
     2701 tcactactta gtgaaaagtt gacagatatt gaagaaaagc ttttctctgt gcacattata
     2761 gagtgaattc acacaaatgt gaactgccag tttaatttct gtaattgtct ctgtttgggg
     2821 gacaggtcat ttattgctaa tgggacagag gtatgtgttt gtgttgttgt atgattatga
     2881 gtatgcatgc taacaggaag agagagggag gagggaggga ggagggggag ggagggaaga
     2941 aaggagggag agagagagtg agagaatgag agagagagtg agagagaaag agttattagt
     3001 gagcaagaga atatgagaga agggccactg acaaccaaat accttgtgat ctttatccta
     3061 aagcatgatt ttccttgaag ctctgtggtt gtttaagaga taattccctc taatatgaaa
     3121 tccctgaaat ataatgacag tatttgaaga tatgtgaata atgtttatcc tatttattta
     3181 tagacttact aaatgagaac actagagaac tttctagaag tcctctagaa tgatacttga
     3241 ttttacagag aggaaaagga gctttgattc tctttaggtt agaataaggt tagtatattt
     3301 ttccctagtc atatttacaa aataccatgt aactttacta caaatatttg agcccagcta
     3361 aaatataccc agaaaattag cataccagtt ttgttttgtt ttattttgtt tttgcatcca
     3421 aacaagcata gtccttctga taagtcactt tagaatggat ctgcctggct cagggttatt
     3481 gttcatgctc agatcatttc cgcaattacc tccagagtcc aactatgcga atgtcacttg
     3541 cagtgctttg atttatgcct tgtattcctc aaagtgtcct tatcctgcta agtcacacct
     3601 cttcctccca gcatttactc taaatgattt ttaatgtttt cgccaatcaa atgtacctca
     3661 cattacaaag ctttgccttg aatgtagatt tttaaaacaa aagtgttaag gctggaaatg
     3721 tagttatcaa agaggaagtt ttaaatgtat ctgttctttt atcagctact ccctccctca
     3781 tggctccctt gaatcactga atagttattt aaacccacat atccaatatg gtactcattc
     3841 ctgggtcttc acaattacag acatcatatc gaaatgattg ggctgacaat tcctttgaag
     3901 gacaaagtaa atatttaatg agaaatatag attctggaga ggcatttgaa aatcacaaat
     3961 gttacgcctc catttcctgt tttccaggct gggtgttctg atttgggagg aaagcagccc
     4021 caaataattt ttaaatatga atctgaaaat aatgttttag aaattatgat ctcgacagtc
     4081 taattaatga gaattgtctg aaagtcctag ctgcatttaa aattatgtaa gttaactaaa
     4141 gccaattttt gaaccccagt cataattgtg taggtaggta aaaagagcat tttaggagga
     4201 aaccgaactt catttcaaga ctgaatctgt tttaaaagaa caatagtggt aaggtaaatc
     4261 ttcatttatt tccctatggt ttacctattt aaacatcgaa gattgaatca aaaggcacct
     4321 ggagcatatt ttggtaactc catttcccac ttggtagttc tatggatgct aactgctgaa
     4381 gaataaactg atcgggattt tcaagggttg tgaacatgtc tcctgatggg aataccgtat
     4441 taagtataaa ggttcaaaat agttgatctc aaaactatac acacacacac aatatatata
     4501 tatatacaca cacacacatg tacacacaca cacacatgca catacacatg gtattgttta
     4561 aaatttattt ctcatgactt agaacaatat aaggattata caaggattca tttcccacca
     4621 tcattcctcc cagtgaagct tttctcaaag tctgagtagg agtttctcct ttctcactgg
     4681 taactatccc acagtggcca ttacatcact agtaatcggt gtgcccagcc ctgcatggaa
     4741 ataaatcaca gaaacataat ttcccagtag acttagtctc ttcaagcctg tgtgcttcta
     4801 gtgtataaaa tctgtaaaaa aaaaaaaaaa aaaaa
//
