LOCUS       NM_001037737            4370 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus aryl hydrocarbon receptor nuclear translocator (Arnt),
            transcript variant 1, mRNA.
ACCESSION   NM_001037737
VERSION     NM_001037737.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Liu,S., Yan,W., Lv,Q., Yang,L., Miao,Y., Hu,Y. and Wei,Z.
  TITLE     3, 3'-diindolylmethane, a natural aryl hydrocarbon receptor
            agonist, alleviates ulcerative colitis by enhancing
            'glycolysis-lactate-STAT3'' and TIP60 signals-mediated Treg
            differentiation
  JOURNAL   Mol Immunol 163, 147-162 (2023)
   PUBMED   37793204
REFERENCE   3  (bases 1 to 4370)
  AUTHORS   Steidemann,M.M., Liu,J., Bayes,K., Castro,L.P., Ferguson-Miller,S.
            and LaPres,J.J.
  TITLE     Evidence for crosstalk between the aryl hydrocarbon receptor and
            the translocator protein in mouse lung epithelial cells
  JOURNAL   Exp Cell Res 429 (1), 113617 (2023)
   PUBMED   37172753
  REMARK    GeneRIF: Evidence for crosstalk between the aryl hydrocarbon
            receptor and the translocator protein in mouse lung epithelial
            cells.
REFERENCE   4  (bases 1 to 4370)
  AUTHORS   Ondrova,K., Zuvalova,I., Vyhlidalova,B., Krasulova,K., Mikova,E.,
            Vrzal,R., Nadvornik,P., Nepal,B., Kortagere,S., Kopecna,M.,
            Kopecny,D., Sebela,M., Rastinejad,F., Pu,H., Soural,M.,
            Rolfes,K.M., Haarmann-Stemmann,T., Li,H., Mani,S. and Dvorak,Z.
  TITLE     Monoterpenoid aryl hydrocarbon receptor allosteric antagonists
            protect against ultraviolet skin damage in female mice
  JOURNAL   Nat Commun 14 (1), 2728 (2023)
   PUBMED   37169746
  REMARK    GeneRIF: Monoterpenoid aryl hydrocarbon receptor allosteric
            antagonists protect against ultraviolet skin damage in female mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4370)
  AUTHORS   Bi,Y., Yang,Q., Li,Z., Wang,Y., Wang,Y., Jia,A., Pan,Z., Yang,R.
            and Liu,G.
  TITLE     Aryl hydrocarbon receptor nuclear translocator limits the
            recruitment and function of regulatory neutrophils against
            colorectal cancer by regulating the gut microbiota
  JOURNAL   J Exp Clin Cancer Res 42 (1), 53 (2023)
   PUBMED   36859266
  REMARK    GeneRIF: Aryl hydrocarbon receptor nuclear translocator limits the
            recruitment and function of regulatory neutrophils against
            colorectal cancer by regulating the gut microbiota.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4370)
  AUTHORS   Korkalainen,M.K., Torronen,A.R. and Karenlampi,S.O.
  TITLE     Comparison of expression of aldehyde dehydrogenase 3 and CYP1A1 in
            dominant and recessive aryl hydrocarbon hydroxylase-deficient
            mutant mouse hepatoma cells
  JOURNAL   Chem Biol Interact 94 (2), 121-134 (1995)
   PUBMED   7828219
REFERENCE   7  (bases 1 to 4370)
  AUTHORS   Li,H., Dong,L. and Whitlock,J.P. Jr.
  TITLE     Transcriptional activation function of the mouse Ah receptor
            nuclear translocator
  JOURNAL   J Biol Chem 269 (45), 28098-28105 (1994)
   PUBMED   7961746
REFERENCE   8  (bases 1 to 4370)
  AUTHORS   Carrier,F., Chang,C.Y., Duh,J.L., Nebert,D.W. and Puga,A.
  TITLE     Interaction of the regulatory domains of the murine Cyp1a1 gene
            with two DNA-binding proteins in addition to the Ah receptor and
            the Ah receptor nuclear translocator (ARNT)
  JOURNAL   Biochem Pharmacol 48 (9), 1767-1778 (1994)
   PUBMED   7980646
REFERENCE   9  (bases 1 to 4370)
  AUTHORS   Reisz-Porszasz,S., Probst,M.R., Fukunaga,B.N. and Hankinson,O.
  TITLE     Identification of functional domains of the aryl hydrocarbon
            receptor nuclear translocator protein (ARNT)
  JOURNAL   Mol Cell Biol 14 (9), 6075-6086 (1994)
   PUBMED   8065341
REFERENCE   10 (bases 1 to 4370)
  AUTHORS   Reyes,H., Reisz-Porszasz,S. and Hankinson,O.
  TITLE     Identification of the Ah receptor nuclear translocator protein
            (Arnt) as a component of the DNA binding form of the Ah receptor
  JOURNAL   Science 256 (5060), 1193-1195 (1992)
   PUBMED   1317062
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ183661.1, AK153355.1, AK145415.1 and AC092203.16.
            
            On Nov 20, 2007 this sequence version replaced NM_001037737.1.
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.149624.1,
                                           SRR1660815.208602.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                CJ183661.1         1-19
            20-1937             AK153355.1         2-1919
            1938-3050           AK145415.1         1848-2960
            3051-4370           AC092203.16        118542-119861       c
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.74 cM"
     gene            1..4370
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /note="aryl hydrocarbon receptor nuclear translocator"
                     /db_xref="GeneID:11863"
                     /db_xref="MGI:MGI:88071"
     exon            1..172
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..2523
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /note="isoform a is encoded by transcript variant 1;
                     HIF1-beta; HIF-1-beta; hypoxia-inducible factor 1-beta;
                     dioxin receptor, nuclear translocator"
                     /codon_start=1
                     /product="aryl hydrocarbon receptor nuclear translocator
                     isoform a"
                     /protein_id="NP_001032826.1"
                     /db_xref="CCDS:CCDS17614.1"
                     /db_xref="GeneID:11863"
                     /db_xref="MGI:MGI:88071"
                     /translation="MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRR
                     SGLDFDDEVEVNTKFLRCDDDQMCNDKERFARSDDEQSSADKERLARENHSEIERRRR
                     NKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLT
                     DQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDV
                     DKLREQLSTSENALTGRVLDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDP
                     VSMNRLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSK
                     FCLVAIGRLQVTSSPNCTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELL
                     GKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKTREWLWMRTSSFTFQNPY
                     SDEIEYIICTNTNVKNSSQEPRPTLSNTIPRSQLGPTANLSLEMGTGQLPSRQQQQQH
                     TELDMVPGRDGLASYNHSQVSVQPVASAGSEHSKPLEKSEGLFAQDRDPRFPEIYPSI
                     TADQSKGISSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQIL
                     AQISRHSNPAQGSAPTWTSSSRPGFAAQQVPTQATAKTRSSQFGVNNFQTSSSFSAMS
                     LPGAPTASSGTAAYPALPNRGSNFPPETGQTTGQFQARTAEGVGVWPQWQGQQPHHRS
                     SSSEQHVQQTQAQAPSQPEVFQEMLSMLGDQSNTYNNEEFPDLTMFPPFSE"
     exon            173..284
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            285..329
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            330..374
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            375..419
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            420..633
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            634..847
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            848..950
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1016
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1102
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1179
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1314
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1389
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1541
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1652
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1728
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1852
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1955
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2103
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2266
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2433
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..4370
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4346..4351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /note="hexamer: AACAAA"
     polyA_site      4370
                     /gene="Arnt"
                     /gene_synonym="bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b;
                     mKIAA4051"
                     /note="major polyA site"
ORIGIN      
        1 ggtgggcgtc cgccatcttg gattccgcgg tagcggtggc ggcggtaagg tgcctaatct
       61 gcggagtggc tcttccctcc cctcccccag ctcggtggcg gctgcccctc ccaccgaggg
      121 tggcgcaggg acggtgccat ctcgaccatg gcggcgacta cagctaaccc agaaatgaca
      181 tcagatgtac catcgctggg tcccaccatt gcttctggaa accctggacc tgggattcaa
      241 ggtggaggag ctgttgtaca gagggctatt aagcgacggt cagggctgga ttttgatgat
      301 gaagtagaag tgaacactaa atttttgaga tgcgatgatg accagatgtg taatgacaaa
      361 gagcggtttg ccaggtcgga tgatgagcag agctctgcgg ataaagagag acttgccagg
      421 gaaaatcata gtgaaataga acggcggcga cggaacaaga tgacagctta catcacagaa
      481 ctgtcagaca tggtacctac atgtagtgcc ctggctcgaa aaccagacaa gctaaccatc
      541 ttacgcatgg ccgtttctca catgaagtcc ttgaggggaa ctggcaacac atctactgat
      601 ggctcctaca agccatcttt cctcactgat caggaactga aacatttgat cttggaggca
      661 gcagatggct ttctgtttat tgtctcctgt gagactggac gggtggtgta tgtctctgac
      721 tcagtgactc ccgttttgaa ccagccacag tctgaatggt tcgggagcac actgtatgat
      781 caggtgcacc cagatgatgt ggataaactt cgagagcagc tctctacatc agaaaatgcc
      841 ctaacagggc gggtcctgga tctgaagact ggaacagtga aaaaggaagg ccagcagtct
      901 tccatgagga tgtgcatggg ctcacgaagg tcgttcatct gccgcatgag gtgtggtact
      961 agctccgtgg accctgtttc catgaataga ctgagctttt tgaggaacag atgcaggaat
     1021 gggcttggct ctgtgaagga aggagaacct cactttgtgg tagtccactg cacaggctac
     1081 atcaaggcct ggccaccagc aggtgtctcc ctcccagatg atgacccaga ggctggccag
     1141 gggagcaaat tctgcctagt ggccattggc aggctgcagg taactagttc tcccaactgt
     1201 acagacatga gtaacatttg tcagccaaca gagttcatct cccgacacaa cattgaaggg
     1261 atattcactt ttgtagacca tcgttgtgtg gctactgttg gctaccagcc acaggagctc
     1321 ttagggaaga atattgtaga attttgtcat cctgaagacc aacaacttct aagagacagc
     1381 tttcagcagg tggtgaaatt aaaaggtcag gtgctgtccg tcatgttccg attccgatct
     1441 aagacccgag aatggctgtg gatgagaacg agctccttta ccttccaaaa cccttattca
     1501 gatgaaattg agtatattat ctgcaccaac accaatgtga agaactctag ccaggaacca
     1561 cggcctacac tgtccaacac catcccaagg tcacagctag gtccgacagc caatttatcc
     1621 ctagagatgg gtacagggca gctgccatcc aggcagcagc agcagcagca cacagaactg
     1681 gatatggtac caggaagaga tgggctggcc agctataatc attcccaggt ttctgtccag
     1741 cctgtggcaa gtgcaggatc agaacacagc aagccccttg agaagtcaga aggtctcttt
     1801 gcacaggaca gagatccaag gtttccagaa atctatccca gcatcactgc agatcagagt
     1861 aaaggcatct cctccagcac tgtccctgcc acccaacagc tgttctccca gggcagctca
     1921 ttccctccta acccccggcc ggcagagaat ttcaggaata gtggtcttac ccctcctgta
     1981 accattgtcc agccatcatc ttctgcaggg cagatactgg cccagatttc acgtcactcc
     2041 aaccctgccc agggatcagc gccgacctgg acctctagct cccgcccagg ctttgccgcc
     2101 cagcaggtgc ccacccaggc tacagccaag actcgttctt cccaatttgg tgtgaacaac
     2161 tttcagactt cttcctcctt cagtgctatg tctcttccgg gtgctcccac tgcctcatct
     2221 ggtactgctg cctaccctgc tctccccaac cgtggctcca actttcctcc tgagactgga
     2281 cagaccacag gacagttcca ggcccggaca gcagagggcg tgggtgtctg gccacagtgg
     2341 cagggccagc agccccatca tcggtctagt tccagtgagc agcatgttca gcagacacaa
     2401 gcacaagcac ctagccagcc tgaggtcttt caagaaatgc tgtccatgct gggagaccaa
     2461 agcaacacct acaacaatga agaatttcct gatctaacta tgtttccccc cttttccgaa
     2521 tagaactatt ggggtgagga taagggtggg gggaaatcac tgtttgtttt taaaagcaaa
     2581 tcttttgtaa acagaataaa agttcctctc ccttcccttc cctcacccct gatatgtacc
     2641 ctttccaccc cttgacttgc tgaagaaacg ttatagaaga aattaaatga atttcccagg
     2701 cttttaggat cctctgaaat tttgaggata ggtgaggcct gaattcctgt ccttatttct
     2761 tctgaccaga agttgcacag acatgatttg tgctggagtc aaggggcaga acagaagaat
     2821 ctgacaggca ctaattggat actgtggctt gtttggatag aaattctgaa tggagtggag
     2881 gaaaggagaa atgccctcat cactgaggat catgaaacac agtaggggtg tgtggagcct
     2941 tgggacgtga gcagtctcca gaaagaggcc tgagagagag catgaacact tatctttgtg
     3001 agtgagtgag tgagtgagtg agtgagtgtg tgtgtgtgtg tgtgtgtgtg tgagagagag
     3061 agagagagag taagagacag agacagatgg ggtgaggggg gcatttgagc tcctatgttt
     3121 tgttccctat tatagagtat atgcaaaatt tgtcccagat cttctttgac tttgtgcttg
     3181 ctctttaaag tgtcctaaga aaattaattt tttcaggtat ttttctattt agtgttgcag
     3241 ccaaagagta tttaaattaa gtctttgctg cacttaaatt catacccagc caaaatggaa
     3301 ctttaggcca acccccagcc ttctgttgct agggttggtc tcctacagac acagtgatca
     3361 agctggatga ctcctgctct ttggtgcttt caactcattg ggaagagctg cagatattac
     3421 caaaataggc tggctacatg aacactgtca gaaatcccag acttgcccac aaggataatg
     3481 ctgcattttt ctgtcagagt cacacatgtt tttctggaga ggttatttct gcatggaaac
     3541 tcaacttctt ggattagcta tcttgagtga aagtcctcac tgacgagtat gcaaaccaaa
     3601 tagcctcctg cacagtagcc tctccttcct gtcaccaaaa cagttttagg tctgctgaag
     3661 tctggtgttc tttgctcctt ctgcaatctt gaaattgggg tttgctttag agcacaaaca
     3721 taagtctgtg ttaggtggac ttaaatccca acagggtcac ttgataatta tagccataga
     3781 aatgcagatg caggtaactg cttttaccct ttaccgtcct caggtgagtc tcctagatca
     3841 acagcctttt tttttttttt tttccttaaa ctggctcctg tcaaagatta agttaatatg
     3901 gaaaagacct cttatgtgta ttgatggggc atgaggagcc caggcaagga gaggctcgtg
     3961 gagaggctga gggaatgtta ctaagtttcc ctccgtttgt ctccagtctg gtgccaggca
     4021 gtagagtgga aaaggaggct atttttttat tctatgtgca cacatacagt atacatatat
     4081 atttatatca cattttactg aaccaaaaag ttgggtttcc aataaaatac ttgtttttta
     4141 ataaccgact tgtttttaac tgtgatctga actataatgt acagttatta cagggcttct
     4201 gaagaagggg gggcggggag aagcttctct gaggggctcg ctctgctttt ccttcacggt
     4261 tttatttttg attgtttttt cttgttgccc atctgtgcta agccttaact gtggcaaaaa
     4321 taatgacatg tagcaaagat tttaaaacaa agtatttttt cttttataaa
//
