LOCUS       NM_019927               6435 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ariadne RBR E3 ubiquitin protein ligase 1 (Arih1),
            mRNA.
ACCESSION   NM_019927 XM_134935
VERSION     NM_019927.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Xiong,T.C., Wei,M.C., Li,F.X., Shi,M., Gan,H., Tang,Z., Dong,H.P.,
            Liuyu,T., Gao,P., Zhong,B., Zhang,Z.D. and Lin,D.
  TITLE     The E3 ubiquitin ligase ARIH1 promotes antiviral immunity and
            autoimmunity by inducing mono-ISGylation and oligomerization of
            cGAS
  JOURNAL   Nat Commun 13 (1), 5973 (2022)
   PUBMED   36217001
  REMARK    GeneRIF: The E3 ubiquitin ligase ARIH1 promotes antiviral immunity
            and autoimmunity by inducing mono-ISGylation and oligomerization of
            cGAS.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6435)
  AUTHORS   Gonzalez-Barbosa,E., Mejia-Garcia,A., Bautista,E., Gonzalez,F.J.,
            Segovia,J. and Elizondo,G.
  TITLE     TCDD induces UbcH7 expression and synphilin-1 protein degradation
            in the mouse ventral midbrain
  JOURNAL   J Biochem Mol Toxicol 31 (10) (2017)
   PUBMED   28621812
  REMARK    GeneRIF: 2,3,7,8-tetrachlorodibenzo-p-dioxin promotes the aryl
            hydrocarbon receptor binding to Ubch7 gene promoter as well as its
            transactivation, resulting in an induction of UbcH7 levels in the
            olfactory bulb, ventral midbrain, hippocampus, striatum, cerebral
            cortex, brain stem, and medulla oblongata.
REFERENCE   4  (bases 1 to 6435)
  AUTHORS   von Stechow,L., Typas,D., Carreras Puigvert,J., Oort,L.,
            Siddappa,R., Pines,A., Vrieling,H., van de Water,B.,
            Mullenders,L.H. and Danen,E.H.
  TITLE     The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by
            initiating a 4EHP-mediated mRNA translation arrest
  JOURNAL   Mol Cell Biol 35 (7), 1254-1268 (2015)
   PUBMED   25624349
  REMARK    GeneRIF: DNA damage induces an increase in ARIH1 protein levels and
            association of ARIH1 with 4EHP. In turn, this causes 4EHP
            recruitment to the mRNA cap, where it is known to compete with
            eIF4E.
REFERENCE   5  (bases 1 to 6435)
  AUTHORS   Kathania,M., Zeng,M., Yadav,V.N., Moghaddam,S.J., Yang,B. and
            Venuprasad,K.
  TITLE     Ndfip1 regulates itch ligase activity and airway inflammation via
            UbcH7
  JOURNAL   J Immunol 194 (5), 2160-2167 (2015)
   PUBMED   25632008
  REMARK    GeneRIF: Ndfip1 regulates itch ligase activity and airway
            inflammation via UbcH7.
REFERENCE   6  (bases 1 to 6435)
  AUTHORS   Haraguchi,S., Tsuda,M., Kitajima,S., Sasaoka,Y.,
            Nomura-Kitabayashid,A., Kurokawa,K. and Saga,Y.
  TITLE     nanos1: a mouse nanos gene expressed in the central nervous system
            is dispensable for normal development
  JOURNAL   Mech Dev 120 (6), 721-731 (2003)
   PUBMED   12834871
REFERENCE   7  (bases 1 to 6435)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 6435)
  AUTHORS   Aguilera,M., Oliveros,M., Martinez-Padron,M., Barbas,J.A. and
            Ferrus,A.
  TITLE     Ariadne-1: a vital Drosophila gene is required in development and
            defines a new conserved family of ring-finger proteins
  JOURNAL   Genetics 155 (3), 1231-1244 (2000)
   PUBMED   10880484
REFERENCE   9  (bases 1 to 6435)
  AUTHORS   Moynihan,T.P., Ardley,H.C., Nuber,U., Rose,S.A., Jones,P.F.,
            Markham,A.F., Scheffner,M. and Robinson,P.A.
  TITLE     The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with
            RING finger/IBR motif-containing domains of HHARI and H7-AP1
  JOURNAL   J Biol Chem 274 (43), 30963-30968 (1999)
   PUBMED   10521492
REFERENCE   10 (bases 1 to 6435)
  AUTHORS   Martinez-Noel,G., Niedenthal,R., Tamura,T. and Harbers,K.
  TITLE     A family of structurally related RING finger proteins interacts
            specifically with the ubiquitin-conjugating enzyme UbcM4
  JOURNAL   FEBS Lett 454 (3), 257-261 (1999)
   PUBMED   10431818
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ176800.1, CA328699.1, CV555932.1, AJ130977.1, CK633971.1,
            AK032640.1, AK047617.1 and AW125831.1.
            
            On Dec 28, 2007 this sequence version replaced NM_019927.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057680.1, SRR1660825.64754.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               CJ176800.1         3-122
            121-776             CA328699.1         68-723
            777-1044            CV555932.1         432-699
            1045-1239           AJ130977.1         742-936
            1240-1760           CK633971.1         135-655
            1761-4504           AK032640.1         184-2927
            4505-6152           AK047617.1         34-1681
            6153-6435           AW125831.1         18-300              c
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 32.01 cM"
     gene            1..6435
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /note="ariadne RBR E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:23806"
                     /db_xref="MGI:MGI:1344363"
     exon            1..396
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1695
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /EC_number="2.3.2.31"
                     /note="UbcH7-binding protein; UbcM4-interacting protein
                     77; protein ariadne-1 homolog; ARI-1;
                     ubiquitin-conjugating enzyme E2-binding protein 1; ariadne
                     1; ariadne ubiquitin-conjugating enzyme E2 binding protein
                     homolog 1; RING-type E3 ubiquitin transferase ARIH1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase ARIH1"
                     /protein_id="NP_064311.2"
                     /db_xref="CCDS:CCDS52817.1"
                     /db_xref="GeneID:23806"
                     /db_xref="MGI:MGI:1344363"
                     /translation="MDSDEGYNYEFDEDEECSEEDSGAEEEEDDDEDEPDDDNLDLGE
                     VELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGPGHEQEEDYRYEVL
                     TAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECH
                     VINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKI
                     MEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPA
                     PDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNW
                     IAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNE
                     DDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSW
                     IEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLE
                     RDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED"
     exon            397..464
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            465..609
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            610..702
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            703..758
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            759..825
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            826..932
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            933..975
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1047
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1178
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1236
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1497
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1610
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..6435
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4861..4866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /note="hexamer: AATAAA"
     polyA_site      4885
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /note="major polyA site"
     regulatory      6414..6419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
                     /note="hexamer: AATAAA"
     polyA_site      6435
                     /gene="Arih1"
                     /gene_synonym="Ari; Ari1; Hari; Hhari; Ubch7bp; Uip77"
ORIGIN      
        1 gtctgccggg cccgcgcgcg tcgcgccatg gactcggacg agggctacaa ctacgagttc
       61 gacgaggacg aggagtgcag cgaggaggac agcggcgccg aggaggagga ggacgacgac
      121 gaggacgagc cggacgatga taacctggat ctgggcgagg tggagctggt ggagcccggg
      181 ctgggcgtcg gcggggagcg agacggactg ctgtgcgggg agactggcgg tggcggcggc
      241 agcgctctgg gtccgggagg cggcggcggc ggtggcggtg gcggcggcgg cccgggacac
      301 gagcaggagg aggattaccg ctacgaggtg ctcacggccg aacagatcct gcagcacatg
      361 gtggaatgta tccgggaggt caacgaggtc atccagaatc cagcaactat cacgagaata
      421 ctccttagcc acttcaattg ggataaagag aagctaatgg aaaggtactt tgatggaaac
      481 ctggagaagc tctttgctga gtgtcatgta attaatccaa gtaaaaagtc tcgaactcgc
      541 cagatgaata caaggtcatc ggcacaggat atgccgtgtc agatctgcta cttgaactac
      601 cctaactcgt attttactgg ccttgaatgt ggacataagt tttgtatgca gtgctggagt
      661 gaatatttaa ctacaaaaat aatggaggaa ggcatggggc agactatttc gtgtcctgcg
      721 catggttgtg atatcttagt ggatgacaac acagttatgc gcctgatcac agattccaaa
      781 gttaagttaa aatatcaaca tttaataaca aatagctttg tagagtgcaa ccgactgtta
      841 aagtggtgtc ctgccccaga ttgccaccat gttgttaaag tccagtatcc tgatgcaaaa
      901 ccagttcgct gcaaatgtgg ccgccagttt tgctttaact gtggtgaaaa ttggcatgat
      961 ccagttaaat gtaagtggtt gaagaagtgg attaaaaagt gtgatgatga cagtgaaact
     1021 tctaattgga ttgcagctaa cacaaaggaa tgtcccaaat gccatgtcac aattgagaag
     1081 gatggcggtt gtaatcacat ggtctgtcgt aaccagaact gtaaagcaga attttgctgg
     1141 gtgtgtcttg gcccttggga accgcatgga tctgcctggt acaactgtaa ccgctataat
     1201 gaggatgatg caaaggcagc aagagatgcc caagagcgat ctagggcggc cctgcagaga
     1261 tacctgttct actgtaatcg ctatatgaac cacatgcaga gtctacgctt cgagcataag
     1321 ctgtatgctc aggtgaaaca gaagatggag gagatgcagc agcacaacat gtcctggatc
     1381 gaggtgcagt tcctgaagaa agcagttgat gtcctctgcc agtgtcgtgc cacactcatg
     1441 tacacttatg tcttcgcttt ctacctcaaa aagaataacc agtccattat ctttgagaat
     1501 aatcaagcag atctagaaaa tgccacagag gtgctttcgg gctaccttga acgagatatt
     1561 tcccaagatt ctctgcaaga tataaagcag aaagtccaag ataagtacag atactgtgag
     1621 agtcgacgaa gggttttgtt acagcatgtg catgaaggct atgaaaaaga tctgtgggag
     1681 tacattgagg actgagactg gccctgcata aaatgaactc tgaaaacttt accatctaga
     1741 gtgctcatgc aattaaaaca aacacaaaca aggaggcact aagcctgttc tgacaccgct
     1801 ggtctgtagt accagaattc tgttttgtta acggaaagtt taagtaaatt atattgtaat
     1861 aaaaaaggta gataaaccat tgtacaacag tattctaggc cgccaacaaa gtgtgacaga
     1921 cacacaaaag ccctccaact ttaacttgta acgtagcttc attctcaacg ctgacttcct
     1981 ttttcttttt cctgagtgta gtacagttaa aatttcaata gctccttgac actgcttttc
     2041 atgtccaaac cagccatttt gttgtacttt ggtaaaggac ctcttcccct tcctcccctg
     2101 cacatacaca cctccacaca cagttggatt cactttctct cataccctca ggtcatgagt
     2161 gaataatgat tattagctac ttgtaaagga aattctttgg agggagaagg ggataggcag
     2221 caagaggatt aaaaaacaaa aaacacaaaa actttgtata tgacttttaa aacaagagga
     2281 caacacagta tttttcaaaa ttgtatatag cgcatatgca tggataaagc aagcgtggca
     2341 cgtgtttgca taatgtttaa ttacaaaaat atttattctt taaaaagctt caagattatg
     2401 tctatttgct gtgcattttc tttcagttgg ctttatcttt ctagggttgg gttgggataa
     2461 aggtgtttgt taagcccctc tgcaagacct aactagagtg ctttccccct cctgaggtca
     2521 gtttgccctt tgtggtttgg ttgtatcttt tgctggccat gggcctcatg cctagaattc
     2581 tccgcttctg gatctagatc caggaagtca cattctaagc aaatgcctcg gcctgttaaa
     2641 gggtttagtt gcttcagttc atgaatgatc ctatcccagc ctactcctct gtggccttga
     2701 cgcccccatt ccagcctgtg tgcccacacc acgcacactg cctgccgtag gctttctagg
     2761 cttcccatca catctcgggg aggcatttca aagtttcttt tcctgtttta tttttcctga
     2821 attgctgctt tgagcctttt aaattttagt tatggctcgt ctctcatccc ttctttatgt
     2881 tagggtgttg aatacaatgt tttcacttta aatataaata aatagccatt cacttagtct
     2941 gagattgtga attaaaatgg tggatactga aattacttgt gtgtgttgcg gtgggttcag
     3001 tttgaaggca aacaccacta gaacatgata ctcccatcca gtacatttga gtaggaatca
     3061 ctgagttttg cttcttttct attgtcagca tataggagaa taatgcattt tagctgtgat
     3121 gtccattttt atgaaatttc tactaaaagc tatgttaaaa gtaaaggatg gtggtggttt
     3181 tattaactat atacttgttt aggccattct gcctgtggta tttttcaaca ggtcagcatc
     3241 cctaagactg tcagttttat ataagagtgc agagtgaact aggcaactag atcaagaggt
     3301 aaaactatta aatgccagtt ttggctgagg acctctttgt cttcctttaa ctgtcctgtg
     3361 cctagggagt gtttaccatt tgtgaggcag ccgtgcctgc tcttgcagtg tacatcttat
     3421 tactccattg ggaagtaggc tcactttcct ctggcttttt gcctaagtta ggctttgctg
     3481 aatcacccta cttttccttt tagaaaggtt gttacatgag atgtacttgc aactgttctt
     3541 ttcccatcag aaatcagtga atgtttgctg agtatattat gctgcttcct caaaccactt
     3601 gtcgctttag gatcaacttg tatctttttc ttccctttcc ttgtcacctc aggtggcaaa
     3661 tctgaagtca agtgtctgct tttccatttt ctctatcccc tatcattctc ccattatcca
     3721 tttgacttta ttttaaataa ttgcatcaat cttaagacct ttatcaacac caaggagatt
     3781 tcaaagaaag ctcaagtaag ttccttttcc tggtgacttt gaaaagcgac cagaattgtc
     3841 acatagatat atgtggtccc ttaaaatgct ttgtgtatgt ggtgtttaaa aagagttgac
     3901 ttcacagtat ctaggtctga gtgtcataag ttctcttcat gctctgccct gcaacaagcg
     3961 tccaaaaaag agtgtgcatc tcatagtctc agtccccacc acggagggat agtgtagttg
     4021 gtgacacttt ctagtcccac atgggacctg aggatattgc actcacaatt tgtgtggatc
     4081 aaggggaact ggctcttctg ctacatccaa gtttttaaca accgtctcca tatctgacag
     4141 catttccaga atttacctgc ctttgtcagt agctagcttc agtgctccca gctgggacag
     4201 gcaagccata ttgttaacag cttccctatt tgacctaccc aagtctctca gagggctcta
     4261 cttaaacatc agggtctccc tgtgttgttg agatcagtta cattgtcagt gcatgcttca
     4321 tttgagcaat tcattgagca acaggtgaat tttcaatgat ttacaataaa attttgatcc
     4381 aaagctcagg agacatacca tagtggtaaa tggagtagca tataacatta ccagactcta
     4441 aattctttgt aatttgctgc aacccagatt gtatatgttt tatgtgtgtt caaataaata
     4501 tattaaagac acacgtgtat acatacacac aaatagatcc aagactgact tgatttgaaa
     4561 tggttgaatc tgcagtgtaa tgtgtggttc acctgttaag taggacttga aatttagaac
     4621 aagaagagta aaaggcttaa atgtaacaaa ttcttttagc tatgtccggt atagagcact
     4681 tgggggatgg gatggggtgg gttggtgaga caatcagatc gataaattga ttaaatgctc
     4741 ctaaccctgt aattttgtgc atagagcacc ttgtgctgtg gaaataactg ttcttagatt
     4801 ttcattgtaa ctggactgtt caggttgccc agagggaaag aacattccta attctaataa
     4861 aataaacttt tattttgtta ttccattagt tacttatgtt tatttttata gttgaaaacc
     4921 gataacttag gtgggtaact gccacctgta taatgtgcct tattcactgt tcctatttaa
     4981 tataaactgt caagatttgg ttcaagttct atgtaaggaa ctgtgaatga gaccattccc
     5041 aacatttagt ttggctaatt gaaacttttc agaagtattt ctgaaagctt tctggtttct
     5101 gcctcttgaa cattgacttt aaattgttgt ttgtgtttta gtagtgatac acacttagat
     5161 gttctgtaca tcttggaatg tcagtttttt atagatgaga tatttaaaac attctagcct
     5221 caaaaggaat cagctttcac ataaaagctg atagtcccta tttatggata gtaacgtggt
     5281 actatcaaac cagacctgac ttttatcaca tctatactct agtttgtcac tttctttttt
     5341 aaaaaaaaaa aaaagtggag taaagattgc tgtttgacgg aatagggaaa taagatttaa
     5401 taacttgcct aaagtttcac aatgtcttct atctgagacc agagtgatat ccaactacct
     5461 ctgtacagtt ttcttattct ggtctcagaa tgacgtagca tccagaacca gaagaagtgg
     5521 ctttttgcat tctttgtcaa ctgtaatgcc cagcagtgcc cctttgagag ggttactgct
     5581 tcatccagga ttgtcctgcc tctccgagaa accagccttg agtagaaaag cccttcccta
     5641 cttgcaggct gggcagctag tccctatgct cacatttgag gtcatagtcc ccaatggcag
     5701 ccagaccacg agttacactc gaaaataaat agcttggtgt aattcaatag ggtatttgca
     5761 gttgttggcc tgtccctttc cagtgtagag aatctggaca ggctgctggg acttgttaca
     5821 ggatctcttt ttatagatga tcgctgaaaa tgcacagagc agctgctaat cttttgagtt
     5881 ctaaaaacaa gttctcaatg tagcccaagt tggtctagga tttatgtagc caaggatggc
     5941 cttgaacttc tgatcttcat gcttctacct cttaagtgct aggatgacag gcatgtgcca
     6001 ctaagtccag cttagcatgt aaccatagga tctcagcatt tattactgca attggagagt
     6061 gtggtaggag tttttttagg gttaattttt aaaccaagta tcagcactgt agcccaggct
     6121 gacctccaat ttagagcttt cctcctgcct ctctgcctcc tattcactgg gattacaggt
     6181 atgcacctat caacttggga gttgagggtt ttttgttttg tttttttttt ttttaaggta
     6241 aacaacagtg ggaaaacggc tccttcataa atgaaaactg tgcactctta agtttttttt
     6301 ttccatttat ctttggggaa gttggtacat attttaaaga atgtgcatct gttagttttt
     6361 caagctgaat taaccaacct agaagctctc acttggaagt caatgtgact aagaataaag
     6421 acaactttag gattt
//
