LOCUS       NM_001372321            4910 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Rho guanine nucleotide exchange factor (Arhgef7),
            transcript variant 4, mRNA.
ACCESSION   NM_001372321 XM_006508841
VERSION     NM_001372321.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Cheng,K., Chahdi,A., Larabee,S.M., Tolaymat,M., Sundel,M.H.,
            Drachenberg,C.B., Zhan,M., Hu,S., Said,A.H., Shang,A.C., Xie,G.,
            Alizadeh,M., Moura,N.S., Bafford,A.C., Williams,R.T., Hanna,N.N.
            and Raufman,J.P.
  TITLE     Muscarinic receptor agonist-induced betaPix binding to beta-catenin
            promotes colon neoplasia
  JOURNAL   Sci Rep 13 (1), 16920 (2023)
   PUBMED   37805544
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4910)
  AUTHORS   Schlienger,S., Yam,P.T., Balekoglu,N., Ducuing,H., Michaud,J.F.,
            Makihara,S., Kramer,D.K., Chen,B., Fasano,A., Berardelli,A.,
            Hamdan,F.F., Rouleau,G.A., Srour,M. and Charron,F.
  TITLE     Genetics of mirror movements identifies a multifunctional complex
            required for Netrin-1 guidance and lateralization of motor control
  JOURNAL   Sci Adv 9 (19), eadd5501 (2023)
   PUBMED   37172092
REFERENCE   4  (bases 1 to 4910)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4910)
  AUTHORS   Cheng,K., Larabee,S.M., Tolaymat,M., Hanscom,M., Shang,A.C.,
            Schledwitz,A., Hu,S., Drachenberg,C.B., Zhan,M., Chahdi,A. and
            Raufman,J.P.
  TITLE     Targeted intestinal deletion of Rho guanine nucleotide exchange
            factor 7, betaPIX, impairs enterocyte proliferation, villus
            maturation, and mucosal defenses in mice
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 320 (4), G627-G643 (2021)
   PUBMED   33566751
  REMARK    GeneRIF: Targeted intestinal deletion of Rho guanine nucleotide
            exchange factor 7, betaPIX, impairs enterocyte proliferation,
            villus maturation, and mucosal defenses in mice.
REFERENCE   6  (bases 1 to 4910)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4910)
  AUTHORS   Kim,T. and Park,D.
  TITLE     Molecular cloning and characterization of a novel mouse betaPix
            isoform
  JOURNAL   Mol Cells 11 (1), 89-94 (2001)
   PUBMED   11266127
REFERENCE   8  (bases 1 to 4910)
  AUTHORS   Kim,S., Kim,T., Lee,D., Park,S.H., Kim,H. and Park,D.
  TITLE     Molecular cloning of neuronally expressed mouse betaPix isoforms
  JOURNAL   Biochem Biophys Res Commun 272 (3), 721-725 (2000)
   PUBMED   10860822
REFERENCE   9  (bases 1 to 4910)
  AUTHORS   Bagrodia,S., Bailey,D., Lenard,Z., Hart,M., Guan,J.L.,
            Premont,R.T., Taylor,S.J. and Cerione,R.A.
  TITLE     A tyrosine-phosphorylated protein that binds to an important
            regulatory region on the cool family of p21-activated
            kinase-binding proteins
  JOURNAL   J Biol Chem 274 (32), 22393-22400 (1999)
   PUBMED   10428811
REFERENCE   10 (bases 1 to 4910)
  AUTHORS   Oh,W.K., Yoo,J.C., Jo,D., Song,Y.H., Kim,M.G. and Park,D.
  TITLE     Cloning of a SH3 domain-containing proline-rich protein, p85SPR,
            and its localization in focal adhesion
  JOURNAL   Biochem Biophys Res Commun 235 (3), 794-798 (1997)
   PUBMED   9207241
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC126800.3 and AC120785.13.
            
            On Aug 29, 2019 this sequence version replaced XM_006508841.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.61559.1,
                                           SRR7345562.759878.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-359               AC126800.3         71241-71599         c
            360-446             AC126800.3         41140-41226         c
            447-531             AC126800.3         18418-18502         c
            532-662             AC126800.3         17002-17132         c
            663-864             AC126800.3         13743-13944         c
            865-953             AC126800.3         8113-8201           c
            954-1048            AC120785.13        180874-180968       c
            1049-1144           AC120785.13        180560-180655       c
            1145-1267           AC120785.13        176105-176227       c
            1268-1406           AC120785.13        172574-172712       c
            1407-1466           AC120785.13        171852-171911       c
            1467-1613           AC120785.13        171031-171177       c
            1614-1700           AC120785.13        168745-168831       c
            1701-1779           AC120785.13        166407-166485       c
            1780-1919           AC120785.13        166128-166267       c
            1920-2144           AC120785.13        163544-163768       c
            2145-2238           AC120785.13        161697-161790       c
            2239-2328           AC120785.13        159379-159468       c
            2329-2505           AC120785.13        156747-156923       c
            2506-2605           AC120785.13        149836-149935       c
            2606-2660           AC120785.13        149479-149533       c
            2661-4910           AC120785.13        146209-148458       c
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 5.73 cM"
     gene            1..4910
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /note="Rho guanine nucleotide exchange factor"
                     /db_xref="GeneID:54126"
                     /db_xref="MGI:MGI:1860493"
     exon            1..359
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..2783
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /note="isoform d is encoded by transcript variant 4; Pak3
                     binding protein; rho guanine nucleotide exchange factor 7;
                     PAK-interacting exchange factor beta; Rho guanine
                     nucleotide exchange factor (GEF7)"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 7 isoform
                     d"
                     /protein_id="NP_001359250.1"
                     /db_xref="GeneID:54126"
                     /db_xref="MGI:MGI:1860493"
                     /translation="MNSAEQTVTWLITLGVLESPKKTISDPEVFLQASLKDGVVLCRL
                     LERLLPGTIEKVYPEPRNESECLSNIREFLRACGASLRLETFDANDLYQGQNFNKVLS
                     SLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSSHSRTSKLLQSQYRSLDMTD
                     NTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVR
                     EIKPSEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTY
                     LRPLQTSDKLSSANTSYLMGNLEEISSFQQVLVQSLEECTKSPEAQQRVGGCFLSLMP
                     QMRTLYLAYCANHPSAVSVLTEHSEDLGEFMETKGASSPGILVLTTGLSKPFMRLDKY
                     PTLLKELERHMEDYHPDRQDIQKSMTAFKNLSAQCQEVRKRKELELQILTEPIRSWEG
                     DDIKTLGSVTYMSQVTIQCAGSEEKNERYLLLFPNLLLMLSASPRMSGFIYQGKLPTT
                     GMTITKLEDSENHRNAFEISGSMIERILVSCTSQQDLHEWVEHLQKQTKVTSVSNPTI
                     KPHSVPSHTLPSHPLTPSSKHADSKPVALTPAYHTLPHPSHHGTPHTTISWGPLEPPK
                     TPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFAVRKS
                     TAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQSSLDSLGRRSS
                     LSRLEPSDLSEDSEYDSIWTAHSYRMGSASRSRKESAPQVLLPEEEKIIVEETKSNGQ
                     TVIEEKSLVDTVYALKDEVQELRQDNKKMKKSLEEEQRARKDLEKLVRKVLKNMNDPA
                     WDETNL"
     exon            360..446
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            447..531
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            532..662
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            663..864
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            865..953
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1048
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1144
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1267
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1406
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1466
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1613
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1700
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1779
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1919
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2144
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2238
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2328
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2505
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2605
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2660
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..4910
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4879..4884
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /note="hexamer: ATTAAA"
     polyA_site      4905
                     /gene="Arhgef7"
                     /gene_synonym="beta-Pix; betaPix; betaPix-b; betaPix-c;
                     Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX"
                     /note="major polyA site"
ORIGIN      
        1 acttgacagc gggccagggc gcgcgcgagc ccgcggagcg gagcgggacc tgctgggccg
       61 gccgggccaa tcgccggcgc gcggccgccc atgggcgcgg aggcggcggg ggcgcgggtg
      121 gccgcggccc gggccgctcc gaggtgatcg cgcgcggcgc cccctctgtg cgcgcagccc
      181 gagccgccgc agcgatgaat tctgccgagc agaccgttac gtggctcatc accctgggcg
      241 tgctggagtc gcccaagaaa accatctcgg accctgaggt cttcttgcag gcgtcgctca
      301 aggacggggt ggtcctatgc cggctgctgg agcgcctgct gcccgggacc atcgagaaag
      361 tctacccgga gccgcggaac gagagcgagt gcctgagcaa catccgcgag ttcctgcgcg
      421 cctgcggggc gtccctgcgc ctagagactt ttgatgcaaa tgatttgtat caggggcaga
      481 actttaacaa ggtcctcagc tccttggtga cactaaataa agtgacagca gacattggac
      541 taggaagtga ttctgtgtgt gctcggcctt cgtcccatcg gataaagtct tttgattccc
      601 tgggatccca gtcttcacac agtaggactt caaaattgct tcagagccag taccgaagct
      661 tggacatgac tgataacacc aacagccaac tggtagtacg agccaagttt aacttccagc
      721 agaccaatga agatgaactc tccttctcaa agggtgatgt catccatgtc acacgagtgg
      781 aggaaggagg ctggtgggag ggcacacaca atggcaggac cggctggttc cccagcaact
      841 acgttcgaga gatcaagcca agtgagaagc ccgtgtcacc caaatcaggg accttgaaga
      901 gccctcccaa agggttcgat acgactgcca tcaacaagag ctattacaac gtggtgctac
      961 agaacatcct ggaaacagag catgagtatt ccaaggagct gcagtctgtg ctgtccacct
     1021 acctgcggcc actgcagacc agtgacaagt tgagttcagc aaacacttca tatttaatgg
     1081 gaaatctaga ggaaatatct tccttccagc aagtgcttgt acagtcccta gaagaatgca
     1141 cgaagtctcc tgaagcccaa cagagagttg gtggctgctt cctgagcctg atgccgcaga
     1201 tgaggaccct gtacctcgct tactgtgcca accacccatc tgctgtgagc gtcctcacag
     1261 agcacagtga ggacctagga gagttcatgg aaacaaaagg tgccagcagc cctgggatcc
     1321 tggtgctgac caccggcctg agcaagccct tcatgcgcct ggacaagtac cccacactgc
     1381 taaaggagct ggagagacac atggaggatt atcatcctga tagacaagat attcagaagt
     1441 ctatgacggc cttcaaaaac ctttcagctc agtgtcaaga agttcgcaag aggaaggagc
     1501 tggagctgca gatcctgacg gagcccatca ggagctggga gggggatgac ataaagaccc
     1561 tgggcagtgt cacatacatg tcccaagtca ccattcagtg tgcgggaagc gaggagaaga
     1621 atgagagata cctcctgctc ttcccaaacc ttctgctcat gttgtctgca agtcccagga
     1681 tgagtggttt catctatcag gggaagctgc caacaacagg aatgacaatc acaaagcttg
     1741 aggacagtga aaaccatagg aatgcatttg agatatcagg gagcatgatc gagcggattc
     1801 tggtgtcctg caccagccag caggacttac acgagtgggt ggaacacctg cagaagcaga
     1861 cgaaggtcac atctgtgagc aaccccacca tcaaacccca ctcggtgcca tcacacacac
     1921 ttccttccca tcctctcact ccatccagca aacacgcgga cagcaagccc gtggcactga
     1981 cgcctgcgta ccacacactc ccccacccct ctcaccatgg caccccacac accaccatca
     2041 gctggggacc cctggagcct ccgaagaccc ccaagccttg gagcctgagt tgcctgcggc
     2101 ctgcacctcc cctccggccc tcagctgctc tctgctacaa ggaggatctc agtaagagcc
     2161 ccaagaccat gaaaaagctg ctgccgaagc gcaagcccga gcggaagcct tcggacgagg
     2221 agttcgctgt gcgcaagagc acagcggcgc tggaagaaga cgctcagatc ctgaaggtta
     2281 tcgaagctta ttgcacaagt gcaaagacgc gccagaccct gaactcaaca tggcaaggca
     2341 ctgacctgat gcataatcac gtcttggctg atgacgacca atcaagtcta gactccttgg
     2401 gtcgtcgcag tagcctttct cgtttggagc cctcagacct ctcggaagac tctgagtatg
     2461 acagtatatg gacagcccat agttacagaa tgggttctgc atcccgttca cgcaaagaat
     2521 ctgctccaca agtgttgctt ccagaagaag aaaaaattat agtcgaagaa accaagagca
     2581 atgggcagac agtgatagag gaaaagagcc tcgtggatac ggtgtatgcg ttaaaggatg
     2641 aagtccagga gttaagacag gataacaaga agatgaagaa gtccttagag gaggaacaga
     2701 gggcccgcaa ggacttagag aagctggtga ggaaggttct aaagaatatg aacgaccctg
     2761 cctgggatga gaccaatcta taggctgact tcattctcat aggctgaaga gcagcagttc
     2821 ctggacatgg ctggggccct tggcttccag cacagtgaca tttaggacta aggaaggtgg
     2881 tctccatcag gcgagcagca gcaatgaggc tgttgctgaa gtagctacat gtgagttata
     2941 gacactgagg tctgggctga gtgtgcacat atctgccact tctccatgca gtgactgacc
     3001 agagtccttt ggcttctgga cttgagttga atctataaat agcatacctg tgtactcctt
     3061 gtaaatactg ggatctgagc acctacctga gatgggactg ggctaaccct cctgctagga
     3121 tttccttctc agcctttctg gccacaggga tctgaagttg tgtttgtgaa gtgttggaag
     3181 cagaaaggtg aagagatgcc gtggcagctg tctgactcgt gtgtagcgca ggctccatgg
     3241 tgccatcggg aacaggcact cgggcacagt gaatttgtgt gtctctgttc ttaactgcag
     3301 tattccagtc tcggctatgg ctgtgcccag ggcatcatgg gttctaggga gggaaactgt
     3361 tacctgcctg tggtgcccgc ctctgctgcc ctgccctgtc ctgtctgggc cttggtcaca
     3421 gtgtgcagaa gtgggttagc cctgtacata gatgtgttat ttattccata ctctctagcc
     3481 ggtgctgccg gccgggcagg gtttggctgc aggctgtttt catccaggag ctctgctctg
     3541 cctctggccg cagtcagcgt tgtccaggcc actgaaagcc ctcctaaccc tctgagcgag
     3601 aataaagcaa tacaaacccc aagtggagca gcacagcgag agccctcaag tgctgtgcct
     3661 gcgccctact ctgctgcctc cccaggccag gcccaggcag gccctggaag gctggtggcc
     3721 tgggcaagcg gccctgcttc tgttaggcca tgcttgcccc tggtttggca acagttaaca
     3781 gtgacctcag gagcttagct gtcccttctt tcctccaaag aggttgtttt caagtcagag
     3841 atactttccc ttagaaaggt gttttgtctt taaacactga agagcaatgt gtgctactgg
     3901 ggcctcagca cagcctcttt ccctaggacg tgacccagcc cccgcccacc cacccacctg
     3961 tggaggtttc tgcgtgggca cttagaggcc gtttgtgctc tctccttctg ccacactcta
     4021 gctggttgca gtgtctatgg gttgtgtctg tcaggatcca ccagagcctc ccacccctgt
     4081 tagcatttac agctcagtgt gcggggtgag aaaagctgag ctgttccttt ccttttggta
     4141 agccctgttt tccaagcact ctgctgaccc agaagacact gcagcaaaag gcagtgtgtt
     4201 tgctgtcaca gcaggtctca actcaggaac aacactcctt gcaactccac tgctggcaca
     4261 ggggcctggg gttgctgggc agcccctcca tcaacaggaa gcaaagacca tgttcgccag
     4321 tgtccttgcc accgccgagg ttgccgtccg tgccccagga caattccttt cagacctgcc
     4381 ttaatgattc gcttggagtg ttccatgagt attcatgttc ccagcatccc taccctggac
     4441 agggtggtta ggctgtcagc acgccgcctt gtctctctga ggatccagtg tcttccaggc
     4501 tctccaggga gcgagaatct gcacccaaga gcagttcttt ttaaaaccgg ccctggtgta
     4561 cacacccact tctctctcct gcgtgtctgc gcggggagct ttgataccaa cagcacaggg
     4621 cgcctctcac caactcctaa gtcctctgtg ttctgtaagg gatgcggggg aatcaagcca
     4681 tttcctgtcg tgtagatgtg aagcactttc cgttctgaac catgctgcgg tgtagcagcg
     4741 tccgaaactc cactgttctc ctccagctgc tgcccatgcg ccctgtgcac ttcatgccaa
     4801 cccccacttt cctgggacac gacaggagac cgcaccgggg tcgactgctc agtgtgtgtg
     4861 tgctacaccc ttgcaattat taaactcctt atttttctta tctcacaaaa
//
