LOCUS       NM_001368773            6519 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Rho guanine nucleotide exchange factor 4 (Arhgef4),
            transcript variant 1, mRNA.
ACCESSION   NM_001368773
VERSION     NM_001368773.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Yoo,K.S., Lee,K., Lee,Y.S., Oh,W.J. and Kim,H.K.
  TITLE     Rho Guanine Nucleotide Exchange Factor 4 (Arhgef4) Deficiency
            Enhances Spatial and Object Recognition Memory
  JOURNAL   Exp Neurobiol 29 (5), 334-343 (2020)
   PUBMED   33154196
REFERENCE   3  (bases 1 to 6519)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6519)
  AUTHORS   Chaboub,L.S., Manalo,J.M., Lee,H.K., Glasgow,S.M., Chen,F.,
            Kawasaki,Y., Akiyama,T., Kuo,C.T., Creighton,C.J., Mohila,C.A. and
            Deneen,B.
  TITLE     Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles
            for Asef during Development and after Injury
  JOURNAL   J Neurosci 36 (47), 11904-11917 (2016)
   PUBMED   27881777
REFERENCE   5  (bases 1 to 6519)
  AUTHORS   Meng,F., Meliton,A., Moldobaeva,N., Mutlu,G., Kawasaki,Y.,
            Akiyama,T. and Birukova,A.A.
  TITLE     Asef mediates HGF protective effects against LPS-induced lung
            injury and endothelial barrier dysfunction
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 308 (5), L452-L463 (2015)
   PUBMED   25539852
  REMARK    GeneRIF: these results demonstrate potent anti-inflammatory effects
            by HGF and delineate a key role of Asef in the mediation of the HGF
            barrier protective and anti-inflammatory effects.
REFERENCE   6  (bases 1 to 6519)
  AUTHORS   Kang,P., Lee,H.K., Glasgow,S.M., Finley,M., Donti,T., Gaber,Z.B.,
            Graham,B.H., Foster,A.E., Novitch,B.G., Gronostajski,R.M. and
            Deneen,B.
  TITLE     Sox9 and NFIA coordinate a transcriptional regulatory cascade
            during the initiation of gliogenesis
  JOURNAL   Neuron 74 (1), 79-94 (2012)
   PUBMED   22500632
REFERENCE   7  (bases 1 to 6519)
  AUTHORS   Kawasaki,Y., Jigami,T., Furukawa,S., Sagara,M., Echizen,K.,
            Shibata,Y., Sato,R. and Akiyama,T.
  TITLE     The adenomatous polyposis coli-associated guanine nucleotide
            exchange factor Asef is involved in angiogenesis
  JOURNAL   J Biol Chem 285 (2), 1199-1207 (2010)
   PUBMED   19897489
REFERENCE   8  (bases 1 to 6519)
  AUTHORS   Kawasaki,Y., Tsuji,S., Muroya,K., Furukawa,S., Shibata,Y.,
            Okuno,M., Ohwada,S. and Akiyama,T.
  TITLE     The adenomatous polyposis coli-associated exchange factors Asef and
            Asef2 are required for adenoma formation in Apc(Min/+)mice
  JOURNAL   EMBO Rep 10 (12), 1355-1362 (2009)
   PUBMED   19893577
  REMARK    GeneRIF: Asef and Asef2 are required for adenoma formation in
            Apc(Min/+)mice.
REFERENCE   9  (bases 1 to 6519)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   10 (bases 1 to 6519)
  AUTHORS   Kawasaki,Y., Senda,T., Ishidate,T., Koyama,R., Morishita,T.,
            Iwayama,Y., Higuchi,O. and Akiyama,T.
  TITLE     Asef, a link between the tumor suppressor APC and G-protein
            signaling
  JOURNAL   Science 289 (5482), 1194-1197 (2000)
   PUBMED   10947987
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157813.6, AC107766.14 and AC107738.16.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849382 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AC157813.6         23326-23406         c
            82-3537             AC107766.14        147108-150563
            3538-3837           AC107766.14        157513-157812
            3838-3964           AC107766.14        170722-170848
            3965-4101           AC107738.16        47382-47518
            4102-4281           AC107738.16        59070-59249
            4282-4461           AC107738.16        60112-60291
            4462-4641           AC107738.16        60471-60650
            4642-4874           AC107738.16        61215-61447
            4875-5004           AC107738.16        61798-61927
            5005-5136           AC107738.16        63853-63984
            5137-5380           AC107738.16        64467-64710
            5381-5458           AC107738.16        65242-65319
            5459-6519           AC107738.16        65834-66894
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 13.34 cM"
     gene            1..6519
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /note="Rho guanine nucleotide exchange factor 4"
                     /db_xref="GeneID:226970"
                     /db_xref="MGI:MGI:2442507"
     exon            1..81
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..5610
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     APC-stimulated guanine nucleotide exchange factor; Rho
                     guanine nucleotide exchange factor (GEF) 4"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 4 isoform
                     1"
                     /protein_id="NP_001355702.1"
                     /db_xref="CCDS:CCDS87817.1"
                     /db_xref="GeneID:226970"
                     /db_xref="MGI:MGI:2442507"
                     /translation="MLGVVHFLRSFFKTPEPGVPPPGEEECEDNQLHPGPADSEEQET
                     NPEDLETLSHKSESRSGSDTKTDPFESASEAESLPGDLTGGVGLSEREAGWGCPEDST
                     VRLPQGRHLVCDEGLHTEEEPDLPSGLKAGSQLSKLHMLTAGRRDEACPCSRVASFVQ
                     ESVPASVCFSKHASSNSSPHLQQARSRGYLDAPVVCPCFRRHCELGQSWPQIHIRTAA
                     AKIPPTGSQHNDFPLWIRTKKGPAPRSTVGTDLFWVHPFLDIACQPRLGTPYLLDTKC
                     HQSTPENLGANAKPPQGYLCWQVKTLVRAHSIASFPLGCPKEPLKQKGTNWPYLREGT
                     EIEPDHPILHKTQSPGPTQLSRLLPTSQSRAPPLQGECKLSDCSFKRASPDTPCVGLP
                     LENQVEQASRSCPAPSHLTSELLKLNSQEEVPRPAECKPGHSPESRLEEPKGVHPDDV
                     ISKQQCLLNISGEAGSQTENYPSMSALQEERKPPDRASLFPQHCCHGEVPVCNRMLAG
                     SWEEESDSACAPRTALKSSVIGSLEAAEEGVVLDLQRNGKALQSLLDFPTLEVSHRNP
                     GSECAQGPGDDPEPSPSGQRQAPGAHGASRHSCKFIMIAVKQEGRQTSTRKAHCLIEP
                     QCGHLPEKGTSATAYESSRASEMPVFGNECELLGCPGAEVDGGALQLLAQGIEHVRVP
                     VSRTSSDDTLSSEETLEGELLGERLDLGSECVHTEQNQELPSSDRKRGRCGTGDAENA
                     EICACAGEVEGPLSRVLASSTTTLEFQEPCGGFQQSVLESEQHPACLQMTPLRTDMPW
                     SREMPGGDIPYSQALTSEDLNPVCPSPEERVPHSPCQGEPPLHHVHQSVSLETKSGVI
                     QVGTSGDLSGEGPSPESYNIKRLKTTESKIKARLALAHKTFTNFFEAKVMEKTNTGSV
                     KGEKEKRRRARGSWRAFLRSKDAEGSKRPALVSGVPEPGFPQLLCSSPPATRGHCEKW
                     TIDTDSHVFGEHWTPLNSPATLSSSHLASPEHRRRSEPTIRRTAAQESSTHLSSGVFP
                     KSSWLTSATSPGAAQIDISFALPCSSACHLTYENQGMPCRPTSPKPRSPMAGAQRMDL
                     CLRGRTSAVSMVSLRSDKDANRSSEVPERPKASKARASLLLSLQTLNQNDQKEDKSQC
                     QCYHGLGTVPFLRDHPGNESYIAQESPPDKKRNCSHDQKTYTEPPHRPVSATEVTRTL
                     PSMSAEQVPREVPLQFRRAPQHSHFSFDDIWLEKAQRRKLKKQMLLRRQMCAELVPED
                     PVQCWRKTTITSPESPHLPRRSHLLSQSAPMGLDHTGWPEPTSDTGMPDGTLDTVCAE
                     ETGSEEDLYEELHNSGHHYSHPRGGGEQLAINELISDGSVVCAEALWDHVTMDDQELG
                     FKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDYEAPRAGAREAD
                     DSGPEAQSCKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKREDMFSEEQLRTI
                     FGNIEDIYRCQKAFVKALEQKFNTERPHLSELGACFLEHQADFQIYSEYCNNHPNACV
                     ELSRLTKLSKYVYFFEACRLLQRMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQH
                     RDFKNVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIHS
                     GELTRVTQPQARSQQRMFFLFDRQLIYCKKDLLRRDVLYYKGRLDMDDLEVVDVEDGK
                     DRDLHVSVKNAFRLYCGTTGDSHLLCARKPEQKQRWLKAFAREREQVRLDQETGFSIT
                     ELQRKQAMLNASKQQATGKPKAVGRPGYLTRHKHPSLPASRPQQQVLVLAEPRRKPSN
                     FWHSISRLAPFRK"
     exon            82..3537
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..3837
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..3964
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3965..4101
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4102..4281
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4282..4461
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4462..4641
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4642..4874
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4875..5004
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5005..5136
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..5380
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5381..5458
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5459..6519
                     /gene="Arhgef4"
                     /gene_synonym="9330140K16Rik; Asef; C230030N03Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcagtcagga gcagcgggtg gccgtagtgc ccgggcagca ccatgctcgg tgtggtgcac
       61 ttccttcgca gcttctttaa gactccagag ccaggtgtgc cccctccagg tgaggaagag
      121 tgtgaagaca accagctcca cccaggccct gcagactcgg aggaacagga aacaaacccc
      181 gaggacttgg agacactgtc ccataagagc gaaagtcgat ccggatccga tacaaaaaca
      241 gatccctttg aaagtgcttc tgaggcggag tccctcccgg gtgacctcac tggtggagtt
      301 ggcctctccg aaagagaagc aggctgggga tgccctgagg actcaacagt tagacttccc
      361 caagggcggc atttggtctg tgacgaaggg ctccatacag aggaagagcc ggatctaccc
      421 tcaggcttaa aggcaggttc tcagctaagt aaattgcaca tgttgactgc tggaagacgg
      481 gatgaagcat gcccctgcag ccgtgtggct agctttgtac aggagtctgt gccagcatct
      541 gtgtgcttct cgaagcatgc cagcagcaac agcagtcccc acctgcaaca ggccaggtct
      601 aggggctatc tggatgcccc tgtggtctgt ccttgctttc gtcgacactg tgaactaggt
      661 cagagctggc cacaaatcca catcaggact gcagcagcaa agatacctcc cacaggctca
      721 cagcacaatg acttccctct gtggatcagg acaaagaagg ggcctgctcc gaggtccaca
      781 gtgggcacag acttgttctg ggttcatccc ttccttgaca tagcctgtca acctcgattg
      841 ggcacacctt atctcttaga caccaagtgt caccagagta ccccagaaaa tcttggtgcc
      901 aatgccaagc cacctcaagg gtacctctgc tggcaggtaa agactctggt cagggcccat
      961 tctatagctt catttccttt aggttgtcct aaagagcctc tgaagcagaa aggtacaaat
     1021 tggccctact tgcgggaagg gacagaaata gaacccgacc acccaatcct tcacaaaacc
     1081 cagtctcctg ggcccaccca gctgtctcgt ttacttccta cttctcaaag cagggcccct
     1141 cctttgcagg gtgagtgcaa gctgagtgat tgtagcttta agagggcatc tccagacaca
     1201 ccctgtgtag gccttccact ggaaaaccag gtagaacagg cttctaggtc atgcccagcc
     1261 ccttcccacc ttacttcaga attgctgaag ctcaacagcc aagaggaagt gcccaggccg
     1321 gctgaatgca agccagggca cagcccagaa agcagacttg aggagcccaa aggtgtccac
     1381 cctgatgatg ttatcagtaa acaacagtgc ctgctaaaca tctccggaga ggcaggaagc
     1441 cagacggaga actacccatc aatgagtgct ctccaggagg agagaaagcc gcctgatagg
     1501 gcctccctct tcccacaaca ctgctgccat ggtgaggtac ctgtgtgcaa cagaatgctt
     1561 gcaggttcct gggaagaaga gagcgatagc gcctgtgccc caaggacagc cctgaaatca
     1621 agtgtcattg gctctttaga agcagcagaa gagggagtgg ttctagacct ccaacgcaat
     1681 gggaaggcct tgcaaagtct tttggacttc ccaacattgg aagtatctca ccgcaaccct
     1741 gggagtgagt gtgcacaggg acctggggat gacccggagc cttccccttc tggccagcgg
     1801 caggctccag gagcccacgg ggcctccagg cacagctgca aattcattat gatagcagta
     1861 aagcaggaag gaaggcagac ttctacaagg aaggctcact gtctcataga accacagtgt
     1921 gggcaccttc cagagaaggg gacaagcgcc acggcttatg agtcttccag ggcctcagaa
     1981 atgcccgtgt ttggcaatga atgtgagctg ctgggatgtc caggggcaga ggtggatggt
     2041 ggggctcttc agctgctggc ccaaggcata gagcacgtga gagtgccagt ctctaggact
     2101 tcttcggatg acactttgag ctcagaggag acactggagg gcgagctgct tggggaaaga
     2161 ttggacttag ggtctgagtg tgtacacacc gagcagaacc aggagttgcc cagctcagat
     2221 cgcaaacgag gaaggtgtgg cacaggcgat gcggagaatg cggaaatctg cgcctgcgcg
     2281 ggcgaggtag agggtcccct ttcaagagtc ttggcttcaa gcaccacaac actggagttc
     2341 caggaaccat gcggtgggtt tcagcagagt gtgctggagt cagagcagca ccctgcctgt
     2401 cttcagatga cccctttaag aactgacatg ccctggtcca gggagatgcc tggcggggac
     2461 ataccctaca gccaggcctt aacctcagag gacttgaacc cagtttgccc tagccccgaa
     2521 gaaagggtcc cccacagtcc ctgccagggg gaaccacctc tgcaccacgt gcaccagagc
     2581 gtatctctgg agaccaaatc tggtgtgatt caagtaggga catctgggga cctcagcggt
     2641 gaaggacctt ccccagaaag ctacaacatc aagagactga aaacaacaga aagcaagatc
     2701 aaagccaggc tggccttagc ccataagacc tttaccaatt tttttgaagc caaagttatg
     2761 gagaaaacaa atactggctc tgtcaaaggc gagaaagaga agcgcaggcg agcccggggc
     2821 tcctggcgtg cgtttctgag aagcaaagat gcggaaggct ccaaacgacc tgctttagtg
     2881 agtggggttc cagaaccagg ctttccccaa ctcctctgct cttccccacc agccaccaga
     2941 ggccactgtg agaagtggac gattgacacc gacagccatg tatttgggga gcactggaca
     3001 cccctaaact cccctgcaac tctgtcctcc agccatctgg cttctccgga acaccggagg
     3061 agaagtgaac cgaccataag acgcactgct gcccaggaaa gcagtacgca cctctcttct
     3121 ggagtcttcc caaagtcttc ctggctgacc tctgccacca gccccggagc agcgcagatt
     3181 gacatcagtt ttgccctccc ctgcagctct gcctgccatt tgacctatga gaaccaaggc
     3241 atgccctgca gacccacaag ccccaaaccc aggagtccca tggctggggc gcagaggatg
     3301 gatctctgcc ttagaggcag gactagtgct gtctccatgg tctctctcag aagtgacaag
     3361 gacgccaaca gaagttcaga agtccccgaa cggcccaagg catccaaggc cagggcgagc
     3421 ctcttactct ctctgcaaac actaaaccag aacgaccaga aggaagacaa gagccagtgc
     3481 cagtgttacc atggcctggg cacagtgccc ttccttaggg accatcctgg gaatgagagc
     3541 tacatcgccc aggaaagccc gccagacaag aagcgcaatt gttcccatga tcagaaaact
     3601 tacacagagc ctcctcacag gccggtatct gccactgaag tgacacggac cctcccctcc
     3661 atgtccgcag agcaagtccc cagagaggtc cctttacagt tcaggagggc ccctcaacac
     3721 tcacacttca gcttcgatga catatggctc gaaaaggctc agaggaggaa gctgaagaaa
     3781 cagatgctgc ttagacgaca gatgtgcgca gagttagtgc ctgaggaccc agttcagtgc
     3841 tggaggaaga caaccatcac ctctccagag tctccgcatc ttcctagaag aagccatctg
     3901 ctctcccaga gtgccccaat gggactggac cacacaggct ggccagagcc cacgtctgac
     3961 actgggatgc ctgacggaac tctggacacg gtgtgcgccg aggagacagg cagtgaggag
     4021 gacctgtatg aagagcttca caactctggc caccactaca gccaccctag aggaggtgga
     4081 gagcaactgg ccatcaatga gctcatcagt gatggcagtg tggtgtgtgc tgaagccctg
     4141 tgggaccatg tcaccatgga tgaccaggag ctgggcttca aagctgggga tgtcatcgaa
     4201 gtgatggatg ccaccaacag ggaatggtgg tggggccgag tggcagatgg cgaggggtgg
     4261 ttccctgcca gctttgtacg gctgcgcgtg aaccaggatg agcccgcgga tgactatgag
     4321 gccccgcggg ccggggctag agaggccgat gacagcggtc cagaggctca gagctgcaag
     4381 gaccagatgc gcacaaatgt catcaatgag atcctcagca ctgagcgaga ctacatcaag
     4441 caccttcgcg acatctgcga gggctatgtc cgccagtgcc gcaaacggga ggatatgttc
     4501 agtgaagagc agctgcgcac catctttggg aacatcgagg acatctaccg ctgccaaaag
     4561 gcatttgtga aggctttgga gcagaagttc aacacggagc ggccacacct gagcgagttg
     4621 ggggcctgct ttctggaaca ccaagcagac ttccagatct attctgagta ctgcaacaac
     4681 caccccaatg cctgcgtgga gctctcccgg ctcacgaagc tcagcaagta cgtgtacttc
     4741 ttcgaggcct gccggctgct gcaaaggatg attgacatct cgctggatgg ctttctgctg
     4801 accccggtgc agaagatctg caagtatccc ctccagctgg cggagctgct caagtacaca
     4861 cacccccagc acagggactt taagaatgtt gaagctgcct tgcacgccat gaagaatgtg
     4921 gcccagctca tcaacgaacg gaaacgaaga cttgaaaaca tcgacaagat tgctcagtgg
     4981 cagagctcca tagaggactg ggagggggaa gaccttctgg tcaggagctc ggaactcatc
     5041 cactcggggg agctgacccg cgtgacacag cctcaagcca ggagtcagca gagaatgttt
     5101 tttctctttg accgccagct catctactgt aaaaaggatc tgcttcgccg ggacgtgctg
     5161 tactacaagg gccggctgga catggatgac ctggaggtgg tagatgtgga agatgggaag
     5221 gaccgagacc tccatgtgag tgtcaagaac gccttccgtc tgtactgtgg taccacgggg
     5281 gacagccacc tactgtgtgc caggaagcca gaacagaagc agcgctggct gaaggccttt
     5341 gccagagaga gggagcaggt gcggctggac caggagacag gcttctccat caccgagctg
     5401 cagaggaagc aggccatgct gaacgccagc aagcagcagg ccacggggaa gcctaaagct
     5461 gtgggaaggc caggatacct gacccgccac aagcatccat cattgcctgc cagccggccc
     5521 cagcagcaag tgttggtgct agcagagccc aggcggaagc catctaactt ctggcacagc
     5581 atcagcagac tggcgccttt ccgcaagtga gccagcatcc atcagacaac ctgacagcac
     5641 tttgtggacc catcctttcc tctctgaggc ctataccatc aggccctctg cctgttttac
     5701 aaactggaag tgaacaggca cagcgtcatg ctgtatgaag tctgggccct gcctctggcc
     5761 ccttccccat ggctagtgtg tgagtaccag cctctgacta tactctggac ctgcgcagag
     5821 cctccatcaa tggtaagcct gcacgccaca gctctaaacc tctggccagg ctgtgcacat
     5881 aggcgcatct gctctggaca ttctttggaa agctatcatc tccattctcc tgtttcctct
     5941 gggtaggaga ccaaaatctg tggccatgag ccacctgcca aagcaggtgt gtgcccctct
     6001 gctctttgtc tgttcaggcc cccagtgcac ttgtaacctg agaggtcagg aaccaggaat
     6061 tatgcactaa ctctacccat acatagctgt ccttatgcct cctggtccct gtagacactg
     6121 ctggctgcca gataccctct ttacttgtga catgtgtaac gagaaaacca tacagtcaac
     6181 tgtgtccctg gttccaggtt agtgacagtt ccctgtagct ggggtggctg cgcacacacc
     6241 cacccactca ctccctgttc actatgattt tggtcgtgtt gtttctctaa cactggagaa
     6301 ttgttaatga cacagaaaga ttgccttacc ctagttaagt gtgagaagcc ataaggactg
     6361 gactctggcc cctcatggcc ggcctcctcg tgaggcacat ggccgggcct gcacatggtg
     6421 cagagcctac aggactcaga agcatggagt cactcttgcg ctgcatcttc tctaatccaa
     6481 aagacacatt taatttcata aagaaacgaa tgaaaagtg
//
