LOCUS       NM_001289687            3663 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus Rho guanine nucleotide exchange factor 3 (Arhgef3),
            transcript variant 3, mRNA.
ACCESSION   NM_001289687 XM_006519578
VERSION     NM_001289687.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   You,J.S., Kim,Y., Lee,S., Bashir,R. and Chen,J.
  TITLE     RhoA/ROCK signalling activated by ARHGEF3 promotes muscle weakness
            via autophagy in dystrophic mdx mice
  JOURNAL   J Cachexia Sarcopenia Muscle 14 (4), 1880-1893 (2023)
   PUBMED   37311604
  REMARK    GeneRIF: RhoA/ROCK signalling activated by ARHGEF3 promotes muscle
            weakness via autophagy in dystrophic mdx mice.
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Liao,L., Qian,Z.Y., Li,X.Y., Yang,D.S., Lei,B.J., Li,H.J. and
            Hong,X.
  TITLE     Disrupting RhoA activity by blocking Arhgef3 expression mitigates
            microglia-induced neuroinflammation post spinal cord contusion
  JOURNAL   J Neuroimmunol 359, 577688 (2021)
   PUBMED   34390950
  REMARK    GeneRIF: Disrupting RhoA activity by blocking Arhgef3 expression
            mitigates microglia-induced neuroinflammation post spinal cord
            contusion.
REFERENCE   3  (bases 1 to 3663)
  AUTHORS   You,J.S., Singh,N., Reyes-Ordonez,A., Khanna,N., Bao,Z., Zhao,H.
            and Chen,J.
  TITLE     ARHGEF3 Regulates Skeletal Muscle Regeneration and Strength through
            Autophagy
  JOURNAL   Cell Rep 34 (1), 108594 (2021)
   PUBMED   33406419
  REMARK    GeneRIF: ARHGEF3 Regulates Skeletal Muscle Regeneration and
            Strength through Autophagy.
            Erratum:[Cell Rep. 2021 Feb 9;34(6):108731. doi:
            10.1016/j.celrep.2021.108731.. PMID: 33567278]
REFERENCE   4  (bases 1 to 3663)
  AUTHORS   Zou,S., Teixeira,A.M., Kostadima,M., Astle,W.J., Radhakrishnan,A.,
            Simon,L.M., Truman,L., Fang,J.S., Hwa,J., Zhang,P.X., van der
            Harst,P., Bray,P.F., Ouwehand,W.H., Frontini,M. and Krause,D.S.
  TITLE     SNP in human ARHGEF3 promoter is associated with DNase
            hypersensitivity, transcript level and platelet function, and
            Arhgef3 KO mice have increased mean platelet volume
  JOURNAL   PLoS One 12 (5), e0178095 (2017)
   PUBMED   28542600
  REMARK    GeneRIF: Data suggest that modulation of ARHGEF3 gene expression in
            humans with a promoter-localized SNP plays a role in human
            megakaryocytes and human platelet function-a finding resulting from
            the biological follow-up of human genetic studies. Arhgef3 KO mice
            partially recapitulate the human phenotype.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3663)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   6  (bases 1 to 3663)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
REFERENCE   7  (bases 1 to 3663)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3663)
  AUTHORS   Arthur,W.T., Ellerbroek,S.M., Der,C.J., Burridge,K. and
            Wennerberg,K.
  TITLE     XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but
            not RhoC
  JOURNAL   J Biol Chem 277 (45), 42964-42972 (2002)
   PUBMED   12221096
REFERENCE   9  (bases 1 to 3663)
  AUTHORS   Schmidt,A. and Hall,A.
  TITLE     The Rho exchange factor Net1 is regulated by nuclear sequestration
  JOURNAL   J Biol Chem 277 (17), 14581-14588 (2002)
   PUBMED   11839749
REFERENCE   10 (bases 1 to 3663)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154327.2, CD539300.1, AK004600.2 and AI853706.1.
            
            On Jan 25, 2014 this sequence version replaced XM_006519578.1.
            
            Transcript Variant: This variant (3) has multiple differences at
            its 5' end, compared to variant 1. These differences result in a
            distinct 5' UTR and cause translation initiation at a downstream
            start codon, compared to variant 1. The encoded protein (isoform 3)
            is shorter and has a distinct N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005517.1, SRR7345562.3762309.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC154327.2         67394-67403
            11-585              CD539300.1         1-575
            586-3640            AK004600.2         531-3585
            3641-3663           AI853706.1         1-23                c
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 16.09 cM"
     gene            1..3663
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /note="Rho guanine nucleotide exchange factor 3"
                     /db_xref="GeneID:71704"
                     /db_xref="MGI:MGI:1918954"
     exon            1..233
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1715
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /note="isoform 3 is encoded by transcript variant 3; Rho
                     guanine nucleotide exchange factor (GEF) 3"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     3"
                     /protein_id="NP_001276616.1"
                     /db_xref="CCDS:CCDS88606.1"
                     /db_xref="GeneID:71704"
                     /db_xref="MGI:MGI:1918954"
                     /translation="MVAKDYPFYLTVKRANCSLEAPLGSGVAKDEEPSNKRVKPLSRV
                     TSLANLIPPVKTTPLKRFSQTLQRSISFRSESRPDILAPRAWSRNATSSSTKRRDSKL
                     WSETFDVCVNQVLTAKEIKRQEAIFELSQGEEDLIEDLKLAKKAYHDPMLKLSIMTEQ
                     ELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGWLPCLSSYDSYCSNQV
                     AAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILRHT
                     PNDNPDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSLIDSSRVL
                     CCHGELKNNRGVKLHVFLFQEVLVITRAVTHNEQLCYQLYRQPIPVKDLTLEDLQDGE
                     VRLGGSLRGAFSNNERVKNFFRVSFKNGSQSQTHSLQANDTFNKQQWLNCIRQAKETV
                     LSAAGQAGLLDSEGLVQGPGTENREPQGETKLEQMDQSDSESDCSMDTSEVSLECERM
                     EQTDASCANSRPEESV"
     exon            234..341
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            342..512
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            513..575
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            576..672
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            673..749
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            750..1007
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1178
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1365
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..3647
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3628..3633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /note="hexamer: AATAAA"
     polyA_site      3647
                     /gene="Arhgef3"
                     /gene_synonym="1200004I24Rik; 9830169H03Rik; Xpln"
                     /note="major polyA site"
ORIGIN      
        1 ggtccactct gcaagaagca gctgtaggtg ttcgcatcgc cgtcgcctgc atcgtggagc
       61 tcgggccctc ttttacccgg gatttttgtt gtcgcctgaa attagcggtg acatctcaaa
      121 gggcaggccc ggcagccgcc atggtggcca aggattaccc cttctacctc actgtcaaga
      181 gggcaaactg cagcttggag gcgcccctgg gcagcggggt ggccaaggac gaggaaccta
      241 gtaacaaaag ggtcaaaccc ctttccagag tcacatcgct agcaaacctc attccacctg
      301 tgaagaccac accattaaag cgcttcagcc agaccttgca gcggtccatc agcttccgca
      361 gcgagagccg ccccgacatc cttgcccccc gagcttggtc cagaaatgcc acctcctcaa
      421 gcacaaagcg gagagacagc aaactgtgga gtgagacctt cgatgtgtgt gtcaatcagg
      481 tgctcacggc caaggagatc aagagacagg aggcgatctt tgaactttct caaggtgaag
      541 aagacttgat agaggacttg aaattagcaa aaaaggccta ccacgacccc atgctgaaac
      601 tctccataat gacggaacag gaactgaatc agatttttgg gaccctggac tctctaattc
      661 ctctccatga agagctcctc agtcagcttc gagatgttcg gaaacccgat ggctcgactg
      721 agcacgttgg tcccatcctc gtgggctggc tgccctgtct cagctcctac gacagctact
      781 gcagcaatca agtcgccgcc aaagccctgc tggaccacaa aaagcaggac cacagagtgc
      841 aggacttcct ccagcgctgt ttagaatcac ccttcagtcg caaactcgat ctctggaatt
      901 tccttgatat tccccggagc cggctagtga agtatcctct gctccttcga gaaatcctga
      961 ggcatacacc aaatgataac ccagaccagc agcacctgga agaagctata aacatcatcc
     1021 agggaattgt ggcagaaatc aacaccaaga ctggggaatc ggaatgccgc tattacaaag
     1081 agcggctcct ttacctggaa gaaggccaga aagattctct gattgacagc tcaagggtcc
     1141 tgtgttgtca tggagagctg aaaaataacc ggggtgtgaa actccatgtt ttcctgttcc
     1201 aagaagtgct ggtgatcacc cgagctgtca ctcacaacga gcagctctgc tatcagttgt
     1261 accgacagcc catccctgtg aaggacctca cgctggaaga cctgcaggat ggagaagtgc
     1321 ggctgggtgg ctccctgcgc ggggccttca gcaacaacga gagagttaaa aacttcttca
     1381 gagtcagttt caaaaatgga tcccaaagtc agacccactc actacaagcc aacgacacct
     1441 tcaacaaaca gcagtggctc aactgtatcc gtcaggcaaa agaaactgtt ctgtctgctg
     1501 ccggccaggc tggcttgctt gactctgagg gactggtcca gggtcccggc actgagaaca
     1561 gggagccaca gggagaaaca aaacttgagc agatggacca atcggacagc gagtcggact
     1621 gcagtatgga caccagtgag gtcagccttg agtgtgagcg gatggaacag acagacgctt
     1681 cctgtgcaaa cagcaggccc gaggagagtg tctgacagaa gactgtgacc ctgctagcag
     1741 caggctgttt cttacctgta cagtgtttgc attccacacg tgaactgttt ggagaggcac
     1801 tttgtaatac tcctgtgcct gtgttggcct agttccctct gttccttgtc cctagtactg
     1861 taactgccgc tcagtctgtg tcagctcaca gcagtcctta ccctgttgct gtatctcaca
     1921 agcatctgga tgagtgggga ggaactgacc gtgttacttt aacttgtcct actttcaaaa
     1981 cagaaagggt ctctaaacta ctgctctgca tggactgggt tgacaaagcc ttcgtcccat
     2041 gcttctctaa tgttcctcta gctctgacct agtagctgta gtcctggcat tgagagagtt
     2101 ttgcagagaa cggagatggc attggaagag aaggcagaaa gtctaattaa ttaaccctta
     2161 gcgtgagctc aggttctgtg ttattcaacc agaaaacatg aaaacatccc tgagggtttc
     2221 tgaggtttaa ttttgcaaag gaggaagcct tgttttgaag cctgtggttt catccagtgc
     2281 aagttgaatg taacaatcta tgaagacctg aaacagaagt ggctttagag agggccaaga
     2341 caaaagcagt tgccagacag gcctggtggc acaggcctac aaacctggca cttgggagca
     2401 gaggcaggaa gatgtggagt tcaagaacag ccttggctac ataccaagtt ctagttcaaa
     2461 ctgagttcca cagtataaac aaaacagagc taaaagcacc tgccagctct gactattcag
     2521 ctgaagtggg ggacagctgt gggaagtgtt tgtcatcgcc agtggcttgc cattagaaaa
     2581 gccatttcta gcctacgaca acagagattg ccaggggccc gagaagatct gccaaggtta
     2641 taggacccca tcaaccttat gtttcatcca cctcggccac tcctcttttc tagtggaaga
     2701 ctgtgactgc acctgggact ctgtttacaa acaagagaga aggacttggg ggtgagaagc
     2761 gggtcactgt cactgaagcc tcctgtgcaa accacggaaa ctgaagttac tccgggcggt
     2821 ctcaggattt cctgctctgc tttcaggctc tggttcttga gctacagtca gactggtggt
     2881 taattcctct tgaggccagt ttcattagct ctcattatct aggatcagct agccaaactg
     2941 gaaacttctt tgttccagcc ttgatttaca gagcaaggat gggttcaaac aaacaaacaa
     3001 acaaacaaac acacaccatg aggaaaataa aatgttcaca aactaaacag cttcttgcaa
     3061 gtgtaaatac ttaggggatt tgtacaaaat tatacccaca ccagcactag tagtaatgat
     3121 tctaaattat tgccaaaaaa gttttttgtt gttgttaatt tctgcatcaa gtttacagaa
     3181 aaggaagcag caaaatacaa agttgtccct ttactgtaca catgggtcat gtgcattcaa
     3241 agaagctgtg agacaagagc aaccaataga aaaacaggta tatgacatgg ttatttttca
     3301 agacatatta tgatcagttc taccctgtaa agcatatttc tgtatttata gattttaaca
     3361 gtggtgaatt tttttctatt acaagtttat ttaaaaccat tttgtttccc aagttgctga
     3421 ataaacaaac ctgctccagg cagacacagt ggaaagctga gacaggacac tggatgatgg
     3481 agaaagcttc aactgttctc tgtagagccg ctggccacgg tggccagtga gtcaaggtct
     3541 gtcagcctgt gggaagagag tggacggatg ttttggtgaa atgaatactt ttgtataatg
     3601 gccttaaact tttctggaag catttcaaat aaattgcatt aaaaacaaaa aaaaaaaaaa
     3661 aaa
//
