LOCUS       NM_001370668            6574 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus Rho/Rac guanine nucleotide exchange factor 18
            (Arhgef18), transcript variant 1, mRNA.
ACCESSION   NM_001370668
VERSION     NM_001370668.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Beal,R., Alonso-Carriazo Fernandez,A., Grammatopoulos,D.K.,
            Matter,K. and Balda,M.S.
  TITLE     ARHGEF18/p114RhoGEF Coordinates PKA/CREB Signaling and Actomyosin
            Remodeling to Promote Trophoblast Cell-Cell Fusion During Placenta
            Morphogenesis
  JOURNAL   Front Cell Dev Biol 9, 658006 (2021)
   PUBMED   33842485
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6574)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6574)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6574)
  AUTHORS   Schell,C., Rogg,M., Suhm,M., Helmstadter,M., Sellung,D.,
            Yasuda-Yamahara,M., Kretz,O., Kuttner,V., Suleiman,H.,
            Kollipara,L., Zahedi,R.P., Sickmann,A., Eimer,S., Shaw,A.S.,
            Kramer-Zucker,A., Hirano-Kobayashi,M., Abe,T., Aizawa,S.,
            Grahammer,F., Hartleben,B., Dengjel,J. and Huber,T.B.
  TITLE     The FERM protein EPB41L5 regulates actomyosin contractility and
            focal adhesion formation to maintain the kidney filtration barrier
  JOURNAL   Proc Natl Acad Sci U S A 114 (23), E4621-E4630 (2017)
   PUBMED   28536193
REFERENCE   6  (bases 1 to 6574)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   7  (bases 1 to 6574)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
REFERENCE   8  (bases 1 to 6574)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6574)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   10 (bases 1 to 6574)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC169677.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-294               AC169677.2         123882-124175       c
            295-419             AC169677.2         112860-112984       c
            420-682             AC169677.2         97092-97354         c
            683-830             AC169677.2         95632-95779         c
            831-942             AC169677.2         92604-92715         c
            943-1000            AC169677.2         91195-91252         c
            1001-1045           AC169677.2         90646-90690         c
            1046-1123           AC169677.2         89901-89978         c
            1124-1226           AC169677.2         89509-89611         c
            1227-1368           AC169677.2         89194-89335         c
            1369-1510           AC169677.2         50122-50263         c
            1511-1623           AC169677.2         48268-48380         c
            1624-1764           AC169677.2         47973-48113         c
            1765-2015           AC169677.2         44743-44993         c
            2016-2141           AC169677.2         42615-42740         c
            2142-2259           AC169677.2         40513-40630         c
            2260-2511           AC169677.2         37874-38125         c
            2512-2588           AC169677.2         36316-36392         c
            2589-2767           AC169677.2         32545-32723         c
            2768-2856           AC169677.2         30522-30610         c
            2857-3042           AC169677.2         29044-29229         c
            3043-3183           AC169677.2         27176-27316         c
            3184-3317           AC169677.2         26698-26831         c
            3318-3365           AC169677.2         26074-26121         c
            3366-3413           AC169677.2         25934-25981         c
            3414-3884           AC169677.2         25327-25797         c
            3885-4218           AC169677.2         24241-24574         c
            4219-4344           AC169677.2         23328-23453         c
            4345-6574           AC169677.2         21011-23240         c
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 1.92 cM"
     gene            1..6574
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /note="Rho/Rac guanine nucleotide exchange factor 18"
                     /db_xref="GeneID:102098"
                     /db_xref="MGI:MGI:2142567"
     exon            1..294
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..4508
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /note="isoform 1 is encoded by transcript variant 1; rho
                     guanine nucleotide exchange factor 18; rho/rac guanine
                     nucleotide exchange factor (GEF) 18"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 18
                     isoform 1"
                     /protein_id="NP_001357597.1"
                     /db_xref="GeneID:102098"
                     /db_xref="MGI:MGI:2142567"
                     /translation="MGSEPKPYAQPLDSAAAASTTKGSCGPRKPENPDFFSTVEDEQE
                     DGFLRHLSESTEDFSLDMGALQGSEYLRDLGLGAPSDLHQSEVIMDPETHRQEARRES
                     SHTSCEGASALPQRRSWERSRSCSGSCRRLSLDASTVDKGACLPRTLASLALNLSGNG
                     QKIWTQGCLPVSGTPAPSSKECSSPEKRLRSKSVPVSCEISCMELASDSDVCTSPVQG
                     LEPPVLECLEKDHVEPEHVLIVQQVLQELRQYHGARQRARMSTSPGGAHSNLTWFEFL
                     SESEDGACKIEKPGKSTRVKRSLSSLRSRVTRQKEKGKSPAHLKDKTQDLPGKRECVN
                     GHQLMRGTFSGHSSCPLCGEPLLNSASLKEHPRTTLLSDGSSPAPSRNVGMTISQKGG
                     LQPTPSPAGSGVRLGPIAGDMDEADSVFLKLKQTADDSLSLTSSNAESVFIEDPYIAS
                     LRCEIESDAHEFEAESWSLSVDLAYAKKQKKEVVKRQDVLYELMQTEAHHVRTLKIML
                     KVYSRALQEELQFSGQAVSRLFPCADDLLDMHSHFLARLKERRQEFLEEGSDRNYVIQ
                     KIGDVLVQQFSGETGERMKEKYAVFCSGHNDAVGQYKLLLQQSKKFQNLIKKIGNFSI
                     VRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYKDLSQALSLIKDIISQVDAK
                     VSEYEKDQRLKEIAAKTDQKSSGKLKNGLTFRKEDMLQQRQLHLEGALCWKSTSGRLK
                     DVLAVLLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASMQ
                     GPEMYEMYTSSKEDRNIWMAHIRRAVESCPDEEEDVFSEAEEKKIAEARTMKLQEFQE
                     RLSLKDQLIAQSLLEKQQIYLEMAQLSGLEESAQNRGLFRGGGDPSETLRGEQILRSA
                     MSEIEGIQSLICQRHLGSTSSQVEEGSVSAGLPRRAETFGGYDSVGSPSKGGSFKRKV
                     SNSDLRPQDWQGPASSPDSRPCDNSAPSGCCEESPQAVEMPSTESLPTVLELELVHRV
                     QTLSQLLLSLQAVIAQQDSYVEMQRTAIQEREKQFRLQSTRGNLLLEQERQRNFEKQR
                     EERAGVEKLQSQLRQEQQRWERERARQQQELELAGARLQEREGEARQMRQRLDQERTE
                     LERQRQAYQHDLERLREAQRAVDRERERLELLRRFKKQNTVPGALPPEVLAEAQPASH
                     PPSFNGDGLEGHSAPAKAPGTQGSAMLHGTGPDNVERPEVARWDSAPPESRPAKSDVP
                     IQLLSATNQIQRQTAVQQQIPTKLAASTKGGKEKGSKSRGSQRWESSASFDLKQQLLL
                     SKFIGKDESASRNRRSLSPVLPAAHGSAPASDPCFPAPSPAPAATPPEAFKFGGTSLP
                     PVSPASSLPTTPLATTDEVSKEDVIFF"
     exon            295..419
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            420..682
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..830
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            831..942
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1000
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1045
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1123
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1226
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1368
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1510
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1623
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1764
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..2015
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2141
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2259
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2511
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2588
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..2767
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2856
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..3042
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3043..3183
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3317
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3365
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3413
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3414..3884
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3885..4218
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4344
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4345..6574
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6556..6561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      6574
                     /gene="Arhgef18"
                     /gene_synonym="A430078G23Rik; D030053O22Rik"
                     /note="major polyA site"
ORIGIN      
        1 acttccccgt gacgcctacg gcaggtacag gctgagggtg gactcccaga tcctcataac
       61 ccacctccat ttgttttctc ctctccttgc tgacccctgc tgcctgctgc tcaatattga
      121 tgaagacaac cataaaaagc cggtggctta gaaagaggaa actgggcaga gttgagccac
      181 tctgtggtta gcctggctca tgtgtccctt gaaaccctgg ttgtgtgaca tgggagctgc
      241 aagagctgtt aacaaagcca gttacctacc tgagcaagaa tcgggaactc atgggctctg
      301 agcccaagcc atatgcccag cccctggact ctgcagctgc agcctcaacc accaagggta
      361 gctgtggacc caggaaacct gagaatccag atttcttctc aactgtggaa gatgagcagg
      421 aagatggttt tctgaggcac ctcagcgaga gcacagaaga cttcagcctg gatatgggag
      481 cccttcaggg cagcgagtac ctccgggatc taggcttagg ggccccttct gacctccatc
      541 agtctgaagt gattatggac cctgagaccc acagacaaga agccagaaga gagtcctccc
      601 acacaagctg tgaaggagct tcggccctgc cacaaagacg cagctgggag aggtcccgga
      661 gctgctcagg gagctgtcga aggctcagcc ttgatgcctc cacggtggac aaaggggcct
      721 gtctcccccg gactctggct agccttgctc tgaatctgtc tggaaatggg cagaagattt
      781 ggacccaagg atgcttacct gtcagtggga caccagcacc ctcaagcaag gaatgtagca
      841 gccctgagaa aaggctaaga tccaagtcag tgcctgtgtc ctgtgagatc agctgtatgg
      901 aactggcttc agactcagat gtgtgcacat cacctgtaca aggccttgaa cccccggtgc
      961 tggagtgttt ggaaaaggac catgtggagc cagaacatgt gttgatagta caacaggtgc
     1021 ttcaggagct gaggcagtat catggggccc gtcagcgagc tcgcatgtcc accagccctg
     1081 gaggagccca ctccaacctc acctggtttg agttcctgtc tgagagcgag gatggtgcgt
     1141 gcaagattga gaagcccggc aagagcacca gagtgaagcg cagcctgagc tccctccgca
     1201 gcagggtcac gaggcagaag gagaagggga agagtccagc acatctaaaa gataagactc
     1261 aggatcttcc agggaagcgg gagtgtgtca atgggcacca gctgatgcga ggaaccttct
     1321 cgggccactc cagctgtcct ctgtgtggcg aacctttgct gaactcagcc tccctgaagg
     1381 agcaccctcg gaccacactt ctgtctgatg gcagcagccc agctccttcc aggaatgtcg
     1441 gcatgaccat ctctcagaaa gggggtctcc agccaacacc gagcccagct ggatctgggg
     1501 tgcgacttgg accaattgct ggagacatgg atgaagctga ctccgtgttt ttaaagttga
     1561 agcagacggc agacgactct ctgtcactca catcctcgaa tgctgagtct gttttcatag
     1621 aagaccctta cattgcttca ctgaggtgtg agatcgaatc agatgcacat gagtttgagg
     1681 ccgagtcctg gagcctctct gtggatttag catatgccaa gaaacagaag aaggaggtgg
     1741 taaagagaca ggacgtccta tatgagctga tgcagacaga ggctcatcac gtgcgcacac
     1801 taaagatcat gctgaaagta tactccagag ccctgcagga agagctgcaa ttcagtggcc
     1861 aggcagtcag ccgtctgttt ccgtgcgctg atgacttact ggacatgcac agccatttcc
     1921 ttgcccgcct taaggagcgc cggcaggagt tcctagagga gggcagtgat cggaattacg
     1981 tcatccaaaa gatcggagac gtcctggttc agcagttctc aggagaaacc ggggagagaa
     2041 tgaaggagaa atacgctgtg ttctgtagtg gccacaatga tgcagtcggt cagtacaagc
     2101 tattgctgca gcaaagcaag aagtttcaaa acttaatcaa gaaaattggc aacttttcca
     2161 ttgtgcgaag gctgggagtg caggagtgca tccttctggt cacacagcgc ataaccaagt
     2221 acccggtgct tgtggaacgc atcatccaga acacagaagc tggcactgag gactacaagg
     2281 acctgagcca agccctgagc ctcatcaaag acatcatctc acaagtggac gccaaagtca
     2341 gtgagtatga gaaggaccag cgcctcaagg agattgcagc caagacggac cagaagtcgt
     2401 ctggcaaact caaaaacggg ctaaccttcc gaaaggagga catgctgcag cagcgccagc
     2461 tccacctgga aggcgccctc tgctggaagt ccacctcagg gcgcctgaaa gatgtcctcg
     2521 ctgtcctgct gaccgatgtg cttttgctgc tgcaagaaaa agatcagaaa tatgtctttg
     2581 cttctgtgga ctcaaagcca cccgtcatct ctctgcaaaa gctcatcgtg agagaggtag
     2641 ccaacgagga gaaggccatg ttcctgatca gcgcctccat gcaggggcca gagatgtatg
     2701 agatgtacac cagctccaaa gaggacagga acatctggat ggcccacatc cgccgggccg
     2761 tggagagctg tcctgacgag gaggaggacg ttttcagtga ggctgaggag aaaaagatag
     2821 ctgaagcccg caccatgaaa ctccaggaat ttcaagagcg actgagcctg aaagaccagc
     2881 tgattgccca gagccttctg gagaaacagc agatctacct ggagatggct cagctgagtg
     2941 ggctggaaga gtcagcccag aaccgaggcc tcttccgagg aggaggagac ccctctgaga
     3001 ccctgcgggg ggagcagatt ctcagatcgg ccatgagtga gattgagggc atccagagcc
     3061 tgatctgcca gcggcacctg ggcagcacca gtagccaagt ggaggaggga agtgtctctg
     3121 caggcctgcc tcggcgggct gagaccttcg ggggctatga cagtgtggga agccctagca
     3181 agggtggcag ctttaaaagg aaggtatcca acagtgacct caggccccaa gactggcagg
     3241 gacctgctag cagcccagac tccaggccct gtgacaacag cgcccccagt ggctgctgtg
     3301 aggaatcgcc acaggctgtg gagatgccta gcacagagag cctgcccact gtcctggaat
     3361 tagagctggt ccaccgggtc cagacactgt cgcagttgct tcttagtctc caggcggtca
     3421 tcgctcaaca ggatagctat gtggagatgc aaagaacggc catccaggag cgcgagaagc
     3481 agttccggct gcagtcaaca cgtgggaacc tgctgctgga gcaagagcgg caacgcaact
     3541 ttgagaagca gcgtgaggag cgcgccggtg tggagaagct gcaaagccaa cttcgccagg
     3601 agcagcagcg atgggagcgg gagcgggcac gccagcagca ggaactggag ctggccggtg
     3661 cgcgactgca ggagcgagag ggcgaggcac gccagatgcg ccagcggctg gaccaggagc
     3721 gcaccgagct agagcgacag cgccaggcct accagcatga cctggagcgt ctgcgtgagg
     3781 cccagcgagc agtggaccgt gaacgtgagc gcctggagct gctgcgcagg ttcaagaaac
     3841 agaacactgt accaggggca ctgcccccgg aggtgctggc agaggcccaa cctgcaagcc
     3901 accctcccag cttcaatggc gatgggctgg aggggcactc ggccccagcc aaagcaccag
     3961 gaacccaagg gagcgcgatg ctacatggca caggacctga caacgtagag cgacccgagg
     4021 tggcccgctg ggacagtgcc ccccctgaga gccggccagc caagagcgat gtgcccatcc
     4081 agctactcag tgccaccaat cagattcaga ggcagacagc cgtgcaacag cagatcccca
     4141 ccaagctggc cgcctccacc aagggtggca aggagaaggg cagcaagagc aggggttccc
     4201 agcgttggga gagctcagcg tccttcgacc tgaagcagca gctgctcctc agcaagttca
     4261 taggcaagga tgagagtgca tcacggaacc gccgttcact gagtcccgtc ctgcctgccg
     4321 cccatgggtc tgcacctgcc tcagacccct gcttcccagc cccaagcccg gccccagctg
     4381 ccacaccccc cgaggccttc aagttcggag gcacatcact accacctgtc tccccagctt
     4441 cctcgctgcc aaccacacca ctcgccacca cagatgaagt tagtaaggaa gatgtcatct
     4501 tcttctgagg ggccatggcc ccaagggcag acccctttct gtcctgatac cctttatgtc
     4561 tctggggatc ctatgggtga ctgtactgtc tggccgtccc cttcccctgc acaagcacag
     4621 ccaggcactt gttcccttga gattgcccaa atggtatgcc ttgagtctca cagctcgcta
     4681 tagatttagt ggcaaaacat gggtagcccc cttgaacaca catacataca tgcatatata
     4741 tgtatatgtg tgtgtgtata tatatatata ttatatatat atgtatgtgt atatatgctc
     4801 aaagttcttt taagttgaag gtggaacccc ccagaggtat aaagcccaag acttgggagc
     4861 cagccaggag acagagcagg gcaggcaggt ctgagaagat accggctcca ggggcagact
     4921 ccataacccc caggaatctc tgaattaaac tgagtcattt tgcaccagaa ggcactagaa
     4981 tcacccagag tccagcgaat ctccacttcc aaagcagcaa ctggcactgg ccacagcctt
     5041 gaccagaaaa tggcctgggc aactccagag gatacagaaa gactcaggca gccccgggca
     5101 atccatggag caatggactg gatctgcggg ttcagaaatc aatctgatga ggggttacca
     5161 actgtcactc cttgcagtag gctggggtct tcccaactct gcttctcagg gggcacctgc
     5221 catgtccgcc catccacctt acctgggatg cagaacccca gtttcagcag ggcagcacag
     5281 accgtgtttg atcgctgtga aatgagcaga tttttcctgg tcctgtcagt gttctcacag
     5341 aaaagcagag ctaacacatt cagggacatg tggaggaata gcctagtgac attgccggtg
     5401 agtagccaca gatggccatc ttggtaccac ctcagcctgt gcaggagtcg ggacagcagc
     5461 tggtcttctg gtctggtctt gttcctagac ctgggcaggt ctgagttcca ggtctaccac
     5521 atttagacct ggctgaggtc cagaatcatc ccttagccag gtttccctgc tataccctac
     5581 cctgaagtac taacaacagg caggaccaag tcctacgctt atggttctta gtctccagag
     5641 cccatcgtcc taagaaacca ctacaaagac cctgccctgt gatacttagg tagacagtac
     5701 cttagatggt ctgcagactg ccaggccatc cccacctctg tggaacaggg aagtgtcaga
     5761 ggctgctggc atgagcaaga gccctccaga gccaagacct aagcactcat gagctcaggg
     5821 agcgactgtg ggcgtagtta ccaagaatcc aaccgcgagc tagtaagaca acagggtgct
     5881 gaaagtggtg gtctctgacg acttccgggt gccaggaatg gagcagggtc ccccttgtcc
     5941 tcatccctag gcctatggac cccagcaccc gaatagctct tactcttgtc cttctgtttc
     6001 tatcttgtgt ccggagtgtg tctatatctt ccagacctca ttgtgccatg ttgctaatca
     6061 gtgttttgtg gtttgttttg ttggtttggg ttttgttgtt gttgttgttt tgtttctgtt
     6121 tttgtactga gatgtatctg agcactttag aaaagctgac ttttaagttc ccactctgtg
     6181 aagagaacat tccagtacct aacaagagct gggtgggctc accaccagac accatgactt
     6241 tttggattta gaagttcaaa tggtttccac ttggccctgg gaaggattca gtgtatcccc
     6301 caccaggtgg ccaggtggtt ctcagctgtg tactgtaaac agtaggacag tgtcatcagt
     6361 tacctgtcac cagcagccat gggtcccttc ctggcagatc tcatttgtga agatggtgag
     6421 ccagcagcat tcaatctccc ttgtcaatcg tgtccatgat cttcgcctac ccctgattat
     6481 tttcacagcg atagcagagc atcctggcaa cagggtattc tggaaccacg ctagaacttg
     6541 tgaccagatt ctagcaataa aattatccag agca
//
