LOCUS       NM_001359232           10480 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Rho guanine nucleotide exchange factor 12 (Arhgef12),
            transcript variant 2, mRNA.
ACCESSION   NM_001359232 XM_006510586
VERSION     NM_001359232.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Weatherly,S.M., Collin,G.B., Charette,J.R., Stone,L., Damkham,N.,
            Hyde,L.F., Peterson,J.G., Hicks,W., Carter,G.W., Naggert,J.K.,
            Krebs,M.P. and Nishina,P.M.
  TITLE     Identification of Arhgef12 and Prkci as genetic modifiers of
            retinal dysplasia in the Crb1rd8 mouse model
  JOURNAL   PLoS Genet 18 (6), e1009798 (2022)
   PUBMED   35675330
  REMARK    GeneRIF: Identification of Arhgef12 and Prkci as genetic modifiers
            of retinal dysplasia in the Crb1rd8 mouse model.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10480)
  AUTHORS   Xie,Y., Gao,L., Xu,C., Chu,L., Gao,L., Wu,R., Liu,Y., Liu,T.,
            Sun,X.J., Ren,R., Tang,J., Zheng,Y., Zhou,Y. and Shen,S.
  TITLE     ARHGEF12 regulates erythropoiesis and is involved in erythroid
            regeneration after chemotherapy in acute lymphoblastic leukemia
            patients
  JOURNAL   Haematologica 105 (4), 925-936 (2020)
   PUBMED   31467124
  REMARK    GeneRIF: ARHGEF12 regulates erythropoiesis and is involved in
            erythroid regeneration after chemotherapy in acute lymphoblastic
            leukemia patients.
REFERENCE   4  (bases 1 to 10480)
  AUTHORS   Chennupati,R., Wirth,A., Favre,J., Li,R., Bonnavion,R., Jin,Y.J.,
            Wietelmann,A., Schweda,F., Wettschureck,N., Henrion,D. and
            Offermanns,S.
  TITLE     Myogenic vasoconstriction requires G12/G13 and LARG to maintain
            local and systemic vascular resistance
  JOURNAL   Elife 8, e49374 (2019)
   PUBMED   31549965
  REMARK    GeneRIF: Here, the authors show that small arteries from mice with
            smooth muscle-specific loss of G12/G13 or the Rho guanine
            nucleotide exchange factor ARHGEF12 have lost myogenic
            vasoconstriction.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10480)
  AUTHORS   Thompson,W.R., Yen,S.S., Uzer,G., Xie,Z., Sen,B., Styner,M.,
            Burridge,K. and Rubin,J.
  TITLE     LARG GEF and ARHGAP18 orchestrate RhoA activity to control
            mesenchymal stem cell lineage
  JOURNAL   Bone 107, 172-180 (2018)
   PUBMED   29208526
  REMARK    GeneRIF: mechanical activation of RhoA in mesenchymal progenitors
            is dependent on LARG, while ARHGAP18 limits RhoA delineated
            cytoskeletal structure in static cultures
REFERENCE   6  (bases 1 to 10480)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   7  (bases 1 to 10480)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 10480)
  AUTHORS   Kuner,R., Swiercz,J.M., Zywietz,A., Tappe,A. and Offermanns,S.
  TITLE     Characterization of the expression of PDZ-RhoGEF, LARG and
            G(alpha)12/G(alpha)13 proteins in the murine nervous system
  JOURNAL   Eur J Neurosci 16 (12), 2333-2341 (2002)
   PUBMED   12492428
REFERENCE   9  (bases 1 to 10480)
  AUTHORS   Swiercz,J.M., Kuner,R., Behrens,J. and Offermanns,S.
  TITLE     Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA
            and growth cone morphology
  JOURNAL   Neuron 35 (1), 51-63 (2002)
   PUBMED   12123608
REFERENCE   10 (bases 1 to 10480)
  AUTHORS   Booden,M.A., Siderovski,D.P. and Der,C.J.
  TITLE     Leukemia-associated Rho guanine nucleotide exchange factor promotes
            G alpha q-coupled activation of RhoA
  JOURNAL   Mol Cell Biol 22 (12), 4053-4061 (2002)
   PUBMED   12024019
  REMARK    GeneRIF: Leukemia-associated Rho guanine nucleotide exchange factor
            promotes G alpha q-coupled activation of RhoA (larg protein)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC173346.1, AC110169.7 and AC160123.2.
            
            On Dec 29, 2017 this sequence version replaced XM_006510586.3.
            
            Transcript Variant: This variant (2) lacks a three-nucleotide
            segment in the central coding region, compared to variant 1. The
            encoded protein (isoform 2) is shorter, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-540               AC173346.1         71645-72184         c
            541-564             AC173346.1         10731-10754         c
            565-650             AC173346.1         9058-9143           c
            651-707             AC173346.1         7007-7063           c
            708-806             AC110169.7         140085-140183
            807-856             AC110169.7         141151-141200
            857-914             AC110169.7         144508-144565
            915-1093            AC110169.7         146597-146775
            1094-1171           AC110169.7         148968-149045
            1172-1291           AC110169.7         149209-149328
            1292-1432           AC110169.7         151337-151477
            1433-1507           AC110169.7         157127-157201
            1508-1600           AC110169.7         159272-159364
            1601-1711           AC110169.7         161434-161544
            1712-1810           AC110169.7         161738-161836
            1811-1853           AC110169.7         165480-165522
            1854-1959           AC110169.7         165829-165934
            1960-2097           AC110169.7         166274-166411
            2098-2130           AC110169.7         166991-167023
            2131-2245           AC110169.7         167318-167432
            2246-2351           AC110169.7         168209-168314
            2352-2564           AC110169.7         169594-169806
            2565-2697           AC110169.7         174259-174391
            2698-2733           AC110169.7         174809-174844
            2734-2888           AC110169.7         175352-175506
            2889-3035           AC110169.7         177259-177405
            3036-3121           AC110169.7         178335-178420
            3122-3247           AC110169.7         180793-180918
            3248-3362           AC110169.7         182892-183006
            3363-3463           AC160123.2         233-333
            3464-3541           AC160123.2         2808-2885
            3542-3617           AC160123.2         4000-4075
            3618-3785           AC160123.2         4478-4645
            3786-3873           AC160123.2         6161-6248
            3874-3960           AC160123.2         6967-7053
            3961-4040           AC160123.2         7169-7248
            4041-4274           AC160123.2         7491-7724
            4275-4745           AC160123.2         9816-10286
            4746-5059           AC160123.2         10975-11288
            5060-5129           AC160123.2         12499-12568
            5130-10480          AC160123.2         13089-18439
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 24.14 cM"
     gene            1..10480
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /note="Rho guanine nucleotide exchange factor 12"
                     /db_xref="GeneID:69632"
                     /db_xref="MGI:MGI:1916882"
     exon            1..540
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     CDS             509..5140
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /note="isoform 2 is encoded by transcript variant 2;
                     leukemia-associated Rho guanine-nucleotide exchange
                     factor; leukemia-associated RhoGEF; Rho guanine nucleotide
                     exchange factor (GEF) 12"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 12
                     isoform 2"
                     /protein_id="NP_001346161.1"
                     /db_xref="CCDS:CCDS90543.1"
                     /db_xref="GeneID:69632"
                     /db_xref="MGI:MGI:1916882"
                     /translation="MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSSSHDF
                     DPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGA
                     AMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIRSGSYVALTVQGRPPGSPQIPLADSE
                     VEPSVTGHMSPIMTSPHSPGAAGNMERITSPVLVGEENNVVHNQKVEILRKMLQKEQE
                     RLQLLQEDYNRTATQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVIAPSRPLGDAL
                     TLSEAEADPGDGLCRTDWSSGDASRPSSDSADSPKSSLRERSYLEEAPERSEGVQDAE
                     PQSLVGSPSTRGAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHV
                     VSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVPVPEEISVDLEK
                     RRPELIPEDLHRLYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKD
                     RGTLEKERACAEQIVTKIEEVLMTAQAVEEERSSTMQYVILMYMKYLGVKVKEPRNLE
                     HKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMEIQKS
                     RHPKHLSTPSSVSPEPQDPAKLRQSGVANEGTDTGYLPASSMSSATSGTALSQEGGRE
                     NDTGTKQVGEASAPGDCLDSTPRVPTTVFDFPPPLLDQVQEEECEVERVAEHGTPKPF
                     RKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPSEIKRQEVINELFYT
                     ERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHVGLNEQMKAVRKRN
                     ETSVIDHIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFHTFVQD
                     AESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPEREKVKKAADHCRQILN
                     YVNQAVREAENKQRLEDYQRRLDTSNLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKV
                     NRDKSIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVR
                     QVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQ
                     PPPCEGDNDEEEPAKLKVEHHDLSVAGLQSPDRVLGLESPLISSKPQSHSLNTPGKSA
                     AEHLFVTATQFAKEQHANGALKEGDGGYPVTIPGPHLPVSEERWALDALRNLGLLKQL
                     LVQQLGLTEKSTQEDWQSFSRYGPASEEVQADSGIRDLENVKACHAREGQMSFKTGTG
                     DIATCDSPRTSTESCAAQDSVILASQDSQASNVLVMDHMILTPEMPPAEPEGGLDESG
                     EHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGNCLILDGYDAVQESSTDEEVASS
                     FPLQPVTGIPAVDSSHQQQHSPQNVHPEGPVSPFTPEFLVQRHWRAMEDTCFEIQSPS
                     CTDSQSQILEYIHKIEADLEHLKKVEESYALLCQRLAGSALPDKLSDKS"
     exon            541..564
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            565..650
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            651..707
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            708..806
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            807..856
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            857..914
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1093
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1171
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1291
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1432
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1507
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1600
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1711
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1810
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1853
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1959
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2097
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2130
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2245
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2351
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2564
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2697
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2733
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2888
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3035
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3036..3121
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3122..3247
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3362
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3363..3463
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3541
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3617
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3618..3785
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3873
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..3960
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            3961..4040
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4274
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            4275..4745
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            4746..5059
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            5060..5129
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     exon            5130..10480
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10457..10462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /note="hexamer: ATTAAA"
     polyA_site      10476
                     /gene="Arhgef12"
                     /gene_synonym="2310014B11Rik; Larg; mKIAA0382"
                     /note="major polyA site"
ORIGIN      
        1 cggcccgggg acatggggcg gcgcgagtcc ggcgcgggac ggtgtgagac gcggcggagg
       61 ctgcggccac gagtggcccg cagccccaga tagagcgggg acccgagggc cgggccccgc
      121 cgactcggga ccgaagccga cgcccgtccc tgagccgatc ctgccccagc tccggaggga
      181 tcccggttcc cgtcctacgg ctcgcgactt ccctctgtga cgaagttttc tctccgtgcc
      241 ttgctgagga tcgttcttag ttcttgagct cgccttgtgg ccgacttgct cggtttgttt
      301 ggggagataa cttgttttgc ctcagcccgc atcccctttc cttgacccct tgcaatcgga
      361 tgttctagat gactgaatgg agttttgagt tggacttttg tgtccctgcc gaaattgggc
      421 ctgatcccag agcactgggg gtggggtgtg gaggtgttac tgtaaaatgc aagttggata
      481 aaaagaagac ctctcgccaa gggccccaat gagtggcaca cagtctacta tcacggacag
      541 gtttcccctc aaaaaaccta taaggcatgg aagtattttg aaccgagagt caccaacaga
      601 taagaaacag aaagttgaac gcagttcatc gcatgatttt gaccccacag atagctcctc
      661 caagaagaca aagtctagtt cagaggagag tagatccgag atctatggtc ttgttcagcg
      721 ctgtgtgatc atccagaaag acgacaatgg cttcgggctg acagtcagtg gagacaatcc
      781 ggtcttcgta caatctgtca aagaagatgg agcagccatg cgtgctggag tacaaacagg
      841 tgatcgaatc atcaaggtga atggtactct ggtgactcat tcaaatcatc tggaggtggt
      901 gaaactaatc agatctggtt cctatgtagc tctcactgtc cagggacgcc cacctgggtc
      961 gccccagatc ccacttgctg attctgaagt agagccatca gtcactggac atatgtctcc
     1021 cattatgaca tcccctcatt cccctggagc agccgggaat atggagagaa tcaccagtcc
     1081 tgtgctcgtg ggggaggaaa acaatgtggt ccataaccag aaagtagaga ttctgagaaa
     1141 gatgttacag aaggaacagg aacggctgca gttgctgcag gaagattaca accgaacggc
     1201 tacccaaaga ttgctcaagg agatccagga ggccaagaag cacatcccgc agctgcagga
     1261 gcagctgtcc aaagccacgg gctctgctca ggacggagct gtaattgcac cctccaggcc
     1321 attaggtgat gccctaacac tcagtgaggc agaagcagat cctggagatg gcctgtgcag
     1381 gactgactgg agcagtggag atgcttctcg gcccagtagt gacagtgctg atagtcccaa
     1441 gagtagcctg agagaaagga gttacctgga ggaagcccca gagagaagtg aaggagttca
     1501 ggacgctgag ccgcagtcgc tggtcggcag tccctccacc cggggcgccc ctcacatcat
     1561 tggagcagag gatgatgatt ttggtactga gcatgagcag atcaatggac agtgcagctg
     1621 tttccagagc attgaattgc tgaagtctcg ccctgctcac ttggctgttt tcttacacca
     1681 tgtagtctca cagtttgacc ctgcaacatt gctctgttat ctctattcag acctgtataa
     1741 gcagacaaac tccaaggaga ctcggcgtgt cttcctggag tttcatcagt tcttcttgga
     1801 ccggtctgca cacctgaagg ttccagttcc tgaggaaatc tccgtggatc tagaaaagag
     1861 aagacctgag cttattcctg aggatctgca tcgcctctac attcagacaa tgcaggagag
     1921 agtccaccct gaagttcaga ggcacttgga ggatttccga cagaaacgca gcatggggct
     1981 gaccttggct gagagtgagc tgactaaact tgatgcagag agagacaagg accggggaac
     2041 tctggagaag gagcgagcat gtgcggaaca gatcgttacc aaaattgaag aagtgttgat
     2101 gactgctcag gctgtagaag aagaaaggag ttccacaatg cagtatgtaa ttctcatgta
     2161 tatgaagtat ttgggagtaa aagtgaaaga acctcgaaac ttggagcaca agcggggtcg
     2221 gatcggcttc cttccaaaga tcaagcaaag catgaagaaa gatagagagg gcgaagaaaa
     2281 agggaagcga agaggattcc ccagcatttt gggaccccca agaagaccaa gccgccacga
     2341 caacagtgca attggcaggg ccatggagat acagaagtca cgccacccga agcacttatc
     2401 cactccctcg tctgtgagtc ctgagcctca ggaccctgcc aagttgcgcc agagtggagt
     2461 agccaatgaa ggaacagaca ctggatacct gcccgccagt tccatgtcct ctgccacctc
     2521 agggactgct ctttcccaag agggagggag agagaatgat acaggaacaa aacaagtggg
     2581 agaagcatcg gcgcctggag actgcttgga cagcacacct cgagtcccca ctactgtttt
     2641 cgacttccct cctcctctat tagatcaagt gcaggaggag gagtgtgaag tagagagggt
     2701 ggcagaacat gggaccccaa aaccctttcg gaagtttgac agcatcgcct ttggagaaag
     2761 tcagagtgag gatgagcagt ttgaaaatga cctggagaca gatccaccca actggcagca
     2821 gcttgttagc agagaagtgt tactgggact gaaaccttct gagatcaaaa gacaggaagt
     2881 gattaatgaa ttgttctaca ctgagagagc tcatgtccgg acactgaagg ttcttgacca
     2941 agtattctat cagcgagtgt ccagagaagg gattctatca ccttcagaac tacgcaagat
     3001 tttttcaaac ttggaagata tccttcaact tcatgttggg ttgaatgagc aaatgaaagc
     3061 tgtccgaaaa aggaatgaaa cttctgttat tgatcatatt ggggaagatc tgctcatctg
     3121 gtttagtgga ccaggagagg agaagctgaa acatgctgct gccacattct gcagtaacca
     3181 acctttcgcc ctggaaatga tcaagtctcg ccagaaaaag gattctcgat tccatacttt
     3241 tgtgcaagac gctgagagta accccctgtg ccgtcgtctt cagctgaagg acatcattcc
     3301 cacccaaatg cagagactca ctaagtaccc gcttcttttg gataacattg ccaagtacac
     3361 agaatggcct ccagaaaggg aaaaggtgaa gaaggctgca gaccattgtc gtcagatctt
     3421 aaattatgta aatcaggctg tcagggaggc agaaaacaag cagcgcttag aagactatca
     3481 gcgtcgcctt gacacctcca atctcaagtt gtccgagtac ccaaatgttg acgagctcag
     3541 gaatttggat ttaacaaaaa gaaagatgat tcatgaaggg ccgttggtct ggaaggtgaa
     3601 tagagataaa agcatcgatc tttatacact gctgctggaa gacatccttg tgctgctaca
     3661 gaagcaggat gacaggctgg tgctaaggtg ccacagcaag attctggcat ccacagccga
     3721 cagcaaacac accttcagcc ctgtcattaa gctgagcacg gtgctggttc ggcaggtggc
     3781 gacagataac aaagctttat tcgtcatctc catgtcagat aatggagccc aaatttatga
     3841 attggtagca cagacggttt ctgaaaagac agtctggcaa gacctcatct gtcgaatggc
     3901 tgcgtcagtg aaggagcagt ctacaaagcc gattcccctc ccacagccgc caccttgcga
     3961 aggagacaat gatgaagaag aaccagccaa gttaaaagtg gagcaccatg atctttcagt
     4021 cgctggtctg caaagtccag acagagtttt gggattagaa tctcccttaa tttcgtcaaa
     4081 gcctcagtct cattcactga atacccctgg caaatcagcg gcagaacatc tctttgtgac
     4141 cgcaacacag tttgcaaagg aacagcatgc aaatggggca ctcaaggaag gtgatggcgg
     4201 gtatccagtc acaatcccag gtccacactt gcctgtgtca gaggagcggt gggcattgga
     4261 cgcactgaga aatttgggct tgttgaagca gttgctggta cagcagttag gtctcaccga
     4321 gaagagtact caggaagact ggcagtcctt ctcacgatac gggccagctt ctgaagaggt
     4381 acaggcagac agtggcatcc gggacttgga aaatgttaag gcttgtcatg ccagggaagg
     4441 acagatgtct tttaaaactg gaactggtga tattgcaact tgtgacagtc cacggacttc
     4501 aactgagtct tgtgctgcac aggattcagt gatactggca tcccaagaca gccaggcaag
     4561 taacgtttta gtaatggacc acatgatcct gaccccagag atgcctcctg cagagccaga
     4621 agggggtctg gatgagagtg gagagcactt ttttgatgcc cgtgaagcac atagtgatga
     4681 taatccatca gaaggtgatg gagcagttaa aaaggaagag aaggatgtta atttacgcat
     4741 ctcaggaaac tgtttgatcc ttgatggcta tgatgcagtt caggagagct ccacagatga
     4801 ggaggttgcc tcctcgttcc ccctgcagcc tgtgacgggc atccccgctg tggactccag
     4861 ccaccagcag caacattccc ctcagaatgt gcatcccgag gggccggttt caccattcac
     4921 cccagaattc ctggtccagc ggcactggcg agctatggag gatacctgtt ttgagatcca
     4981 gagtccctca tgtacagatt cacaaagcca aatcctggag tacattcata aaatagaggc
     5041 tgacctggaa cacttaaaga aggtggagga aagttacgct cttctttgcc aaaggctggc
     5101 tggctcagcc ctccccgaca agctctcaga taaaagttag agccccgtgt cctggaggtg
     5161 actgcagctt gttggatttt gagtgtcggc cctgtatcaa ccacatcctg gctccagtgt
     5221 ggacacagag agagtgcgac agagggatct gcctggaacc acctggcctc agccaggccc
     5281 caggggcccc gcggggacta gttcttcctt ctggcagctg cacacatctg tcagtgaggg
     5341 aatgccaggt ctctcacgcc tctcctcact atcagaaatg cattttgatt cagagtaaaa
     5401 accaaatgta tagagctttg ggtgtaggat ataaaattgt acgtaggtaa gaaaaagaga
     5461 aaatcagatg tatttatttg acttcattcc gtatttgaaa gcacacttta atttttactt
     5521 tgccttgttt tgtttttaat tgagtagtga gagttttagc cctttgtatt tagcacaccc
     5581 aaggatcaat tgttcctgaa gccgaggtca ggctggggat ggagaggtta gtagacaggc
     5641 agtgcagaag agcaaagagg actacctggc cctcgctaat ctgtaaccca gccctcctgt
     5701 ccagaaggct gcctttctga ggatcatctc agaggtgctg catgactcct gtgtgtggat
     5761 gccatgagct gtgtgtgccc gggtgctcgg agcaggagga cctcctccca gcccaaggcg
     5821 agcatctcct actgggttcc attcccagga aacaggaatc ctcagtacac taaatggcag
     5881 gcacttgaaa atgggtggat ttcccttgtc gttgtctttt ctattgatag ttgggatttg
     5941 ggagagaaga aagcaaattt ttattttctt gattataaat ttgttattct tggtattgtt
     6001 acatttgtat aattatacat tgactggcac ttgtatgaaa tattccctgc agagtgagca
     6061 ggaaatagga acaaaggaat ggaatgctta gtggtggtgg gggtgctatg gggggtgggt
     6121 gggagagttg cagacttgag gaggttttcg aaactgaatc actactgagt tgaaccatgg
     6181 ctgtcactag ccacgggtac tgctgattat aaggccagta agaaaattag tacctggatg
     6241 tttgactctt aaggttttgc actgatggtg ccaaagggct ctgagtcaca cgcagagcca
     6301 agggagggca gaggaaggca tgaaggagag ttgctcgcat cgcatccctt cagagagctt
     6361 tctctgacct cactgccata gccctgtctg tctggtgcca ggactggcct tgggaacggg
     6421 gaccagcggt gagcagttgc agccgccaag gtgctgacgt gctgcagtgt ggacagcgcc
     6481 tgtggagggc cagctggtca gactgtggcc tgacaactct taattctgat ggtgttcagt
     6541 ggcttgcttt gtgcatttga taaatatgtt tttagagtgt tctgtaaaaa aaaaaaatca
     6601 tttgtctaat ctaaattctg ttaagaagga gctcacatgt tgcttacatc ttgcaggagg
     6661 gggcctctga actctggggg cagtcaggat gtaattttat tctcccctta atgcccctca
     6721 cacaaaggaa gctcactgtc gccaagaata gtcccccagc tgcttttatg aacacctcta
     6781 caacttaaca agctcatctg tctgggtcca gattctcgtt tctgtcctct aagctcttac
     6841 gtcgattggc taggacccct gtacctcatc tataaattgg aatcatattt tgagtcatag
     6901 tcaaccctaa ctcataatgg agctttgaga ttttggagta gaatctaata tataagtgaa
     6961 gtttgggatt tggacctccc tgtatctctt aggagaagca agtagagacc gaatggttcc
     7021 gttgtcttcc aggcagcctc ttacactaca ccatctgggt agctcatcag acagcagtgt
     7081 tccgacctca gacactacag atccctccgt ggttctccag cacagttcag ccggaactct
     7141 gttctcctgc ttgtttactt ccctctttct gagtcccagc ctctcagagt cacagcaggg
     7201 cttactggac aggtactctg gcgttgctcc agtacttcca aataaatgca tatcattaca
     7261 ccttcagatg aaacctcaga atgaagtgca ttctgaaaga caacagcaac aaaccacttt
     7321 gtaagttttt tttttactct accagcctta cttcttgcct gttcccctcc ctcgggcctc
     7381 ctgtgccttc cactccattc tggccattag aatccatttg actttgacat tacatctccc
     7441 ttccgtggtg gcgacagcag tgttcctcct gttctggaat gagatggtca ttgtcatagt
     7501 cctcttaaag gtgggtgaca agaaggtgca gtgtacttct gctccaaaga acactaggca
     7561 agcggaaagc catgtgtgcc atgggtgcaa cctgtgaccc tgctctcctg tgagcttagt
     7621 ggctctcctg tcactaactg gcaggttatg tgtggagtgt caccttttca gagagaaaaa
     7681 ctaaccaagc caaaccagca aaccctccaa gtactgtaat gtaaaggccg gctttggcct
     7741 tccccacctg tatagtttca tggcagcccc ctggactgtt gagttctgct gctttcacaa
     7801 cctaaactgt aactatttat tgtatcacac gcaggttgtt catgagtatt ttttcctttg
     7861 tttccccaaa caacttcttt tttttttttt aagtcttgga agcttatggt ttattgtgga
     7921 ttgctctcag gagagtaaga gacaagaaaa gcaacatata tgtgggggga ggggttggag
     7981 tggtggtgga gcaaagccag ctggagacag gctccagcct agacccttga agaccagaga
     8041 gcagcaatgc accatactct tgatttcatg gggagaaagg gggatggacc cccaggccag
     8101 gaggtgtaac tcctagctgc acctgtttcc atatgaccaa gagtaatcat ccgggaatga
     8161 gagagtctat aaagaacact cattgctgct gcaggtgagc cagacatggc caggtgacac
     8221 cagccatgtc tctgatttca gtcaactctt ttcatttgtt tcctgaatag caaacacttc
     8281 cttgaaactc ttctcaaact tggactccag tctaagtccc atttcgccaa acttagggtc
     8341 cttgaacgtg attttgtgat tccggaagat gagcctcatg tcttgcacaa agtcttctac
     8401 ttggcagtaa gcctggtcac tcagcttttt cttgattatg ttcaaccaca taggctcttg
     8461 cacacccaca gtcatctctc taaaataata ataatatggc atcttggaaa aaaaggagct
     8521 ctcagaacag caatagactc tcaagagaac atactcgcac ttcaactgtt cctggggagc
     8581 catctccctc tgcagtatct cagattctgg aagactcggt tgacttccta aggactgcat
     8641 cctacagaag atgcaactcc atggtgttat ctcagcttcc actgttggga tatgacagtc
     8701 ctcatggaag gctctggaac aagtatcaca gcagaagagt attcctccat cccggcacac
     8761 ctcacattca tctgagttct gaatactagg gtcagctggg gaacgatgca agttctgtgt
     8821 tctctgctct tttcttttgc gatgtatccg tggaggattg ggtagaaatt ttctctggat
     8881 caggagtttg aggggccaat tatggcagcg caggctcagt ttccagttct ttgactttcc
     8941 atagcctccc atcatttcaa attccgaggg tgtgaaccag tttccattct gacactgtat
     9001 ggacttcaca gatatccctt gtttgaattt atccttgtgt aacactccct tcaagttacc
     9061 acacgtcacg ggaagcaact cagcactaaa atcggcattt tcttttctgc gtcttcttag
     9121 acccctgatc ttagcagtgc gtcctgggtt ctttttcatt ttctttctct tgaaagctgc
     9181 ctggacaaca gctattctgc cacgttttct ctgtacactc attggctttc tccattttgg
     9241 ttttgaccag ttgtggcctt tcttttttct tctctgcttc ttgggttttc ttgcaggaat
     9301 gttcctcctg gtgcccacac attctccttg gctgcttccc atccttgctt ccaggtcttc
     9361 tggcacatat gttggggaac acatgacaca ggaacacttc ttttcacgtg tgggttgctg
     9421 tgctcctggg ccaaaattgt gggtgtacat aggtttgtta tctaattttg taatactggg
     9481 gaccaagctc agggtctcat ttgtgcaagg caagcagtct accagtgccc caaacaactt
     9541 ctaatgacct ctagaaagaa caagcccttt tttagaaagt attttgtttc attgtctttt
     9601 ggggagtgaa gaagagaaaa gtccttgttg ccagaatttt agaagctaaa agactttatg
     9661 ctgcctctac tgcaggggct cgttttcccg tcgctcctgg gcactggcgc tggcctgctg
     9721 cctgcagctg gtaccctgcc caagctgtct tctctgcagc cgcctgcttg agcttgctct
     9781 gtgcctgctt ccctccacgc tccgaaaacc atgatctaca aaaacagact tctgcaaggt
     9841 ctcgctcgcc tgcccacccg ccccctaacg ttgtgttggt tttggttttt gtttttgttt
     9901 ttgacagttc acccccattt agtgtgagct gtaaaactgt actttattta aaggcgtgct
     9961 cttgttcccg tctggctgcc ttccccatgg gtgctgaggg tttgggtaat acactgatca
    10021 ggaaaaggtg gtcttctcag aaaccagtcc ctccctccca gccattgcca ggtgccaccc
    10081 gctaagcaga catactacag caggggaccg tagcgtgcac actgttggtg gtttttaatg
    10141 agcctctctt tccccactag gctgtctcac tttacttgtt caccatacaa tcatttactc
    10201 tttaacaaaa attgctttta agagaaatct ggaactatct ttaaaaaaaa aaccttatta
    10261 ataatcatgt atttttactg atcacatttt gaaatgccta aaagactttt attgctctaa
    10321 ttatccagat gcacctttgt aaaaagctct tttatgaatt agctgataag gctgtatgtt
    10381 tctggaacaa aatattgggt catctaaaac tttctgtttt ctggggtctg ggaaaataga
    10441 aaataagaat tcaaatatta aataagctta aaagaactga
//
