LOCUS       NM_001130150            3407 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus Rho guanine nucleotide exchange factor 1 (Arhgef1),
            transcript variant 1, mRNA.
ACCESSION   NM_001130150
VERSION     NM_001130150.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Liu,D., Winer,B.Y., Chou,M.Y., Tam,H., Xu,Y., An,J., Gardner,J.M.
            and Cyster,J.G.
  TITLE     Dynamic encounters with red blood cells trigger splenic marginal
            zone B cell retention and function
  JOURNAL   Nat Immunol 25 (1), 142-154 (2024)
   PUBMED   38049580
REFERENCE   3  (bases 1 to 3407)
  AUTHORS   Liu,D., Duan,L., Rodda,L.B., Lu,E., Xu,Y., An,J., Qiu,L., Liu,F.,
            Looney,M.R., Yang,Z., Allen,C.D.C., Li,Z., Marson,A. and
            Cyster,J.G.
  TITLE     CD97 promotes spleen dendritic cell homeostasis through the
            mechanosensing of red blood cells
  JOURNAL   Science 375 (6581), eabi5965 (2022)
   PUBMED   35143305
REFERENCE   4  (bases 1 to 3407)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3407)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3407)
  AUTHORS   Girkontaite,I., Missy,K., Sakk,V., Harenberg,A., Tedford,K.,
            Potzel,T., Pfeffer,K. and Fischer,K.D.
  TITLE     Lsc is required for marginal zone B cells, regulation of lymphocyte
            motility and immune responses
  JOURNAL   Nat Immunol 2 (9), 855-862 (2001)
   PUBMED   11526402
REFERENCE   7  (bases 1 to 3407)
  AUTHORS   Chu,C.C. and Paul,W.E.
  TITLE     Expressed genes in interleukin-4 treated B cells identified by cDNA
            representational difference analysis
  JOURNAL   Mol Immunol 35 (8), 487-502 (1998)
   PUBMED   9798653
REFERENCE   8  (bases 1 to 3407)
  AUTHORS   Aasheim,H.C., Pedeutour,F. and Smeland,E.B.
  TITLE     Characterization, expression and chromosomal localization of a
            human gene homologous to the mouse Lsc oncogene, with strongest
            expression in hematopoetic tissues
  JOURNAL   Oncogene 14 (14), 1747-1752 (1997)
   PUBMED   9135076
REFERENCE   9  (bases 1 to 3407)
  AUTHORS   Glaven,J.A., Whitehead,I.P., Nomanbhoy,T., Kay,R. and Cerione,R.A.
  TITLE     Lfc and Lsc oncoproteins represent two new guanine nucleotide
            exchange factors for the Rho GTP-binding protein
  JOURNAL   J Biol Chem 271 (44), 27374-27381 (1996)
   PUBMED   8910315
REFERENCE   10 (bases 1 to 3407)
  AUTHORS   Whitehead,I.P., Khosravi-Far,R., Kirk,H., Trigo-Gonzalez,G.,
            Der,C.J. and Kay,R.
  TITLE     Expression cloning of lsc, a novel oncogene with structural
            similarities to the Dbl family of guanine nucleotide exchange
            factors
  JOURNAL   J Biol Chem 271 (31), 18643-18650 (1996)
   PUBMED   8702517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161441.21.
            
            On Oct 13, 2022 this sequence version replaced NM_001130150.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172354.1, AK157056.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AC161441.21        50323-50429         c
            108-149             AC161441.21        45694-45735         c
            150-230             AC161441.21        45517-45597         c
            231-344             AC161441.21        45027-45140         c
            345-443             AC161441.21        41296-41394         c
            444-486             AC161441.21        41099-41141         c
            487-733             AC161441.21        40650-40896         c
            734-763             AC161441.21        40282-40311         c
            764-863             AC161441.21        39953-40052         c
            864-960             AC161441.21        39735-39831         c
            961-996             AC161441.21        37398-37433         c
            997-1134            AC161441.21        36510-36647         c
            1135-1240           AC161441.21        35451-35556         c
            1241-1252           AC161441.21        34920-34931         c
            1253-1420           AC161441.21        34100-34267         c
            1421-1566           AC161441.21        33675-33820         c
            1567-1713           AC161441.21        31434-31580         c
            1714-1796           AC161441.21        31044-31126         c
            1797-1922           AC161441.21        30785-30910         c
            1923-2037           AC161441.21        30530-30644         c
            2038-2138           AC161441.21        30341-30441         c
            2139-2216           AC161441.21        30010-30087         c
            2217-2292           AC161441.21        29717-29792         c
            2293-2460           AC161441.21        29481-29648         c
            2461-2548           AC161441.21        28568-28655         c
            2549-2635           AC161441.21        28389-28475         c
            2636-2703           AC161441.21        28132-28199         c
            2704-2790           AC161441.21        27964-28050         c
            2791-2954           AC161441.21        27504-27667         c
            2955-3054           AC161441.21        27328-27427         c
            3055-3407           AC161441.21        26859-27211         c
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 13.52 cM"
     gene            1..3407
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="Rho guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:16801"
                     /db_xref="MGI:MGI:1353510"
     exon            1..107
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            108..149
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..3065
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="isoform a is encoded by transcript variant 1; lbc's
                     second cousin; lymphoid blast crisis-like 2; Rho guanine
                     nucleotide exchange factor (GEF) 1"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 1 isoform
                     a"
                     /protein_id="NP_001123622.1"
                     /db_xref="CCDS:CCDS52142.1"
                     /db_xref="GeneID:16801"
                     /db_xref="MGI:MGI:1353510"
                     /translation="MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANSEDQNSQF
                     QSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFYHSFL
                     EKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEVVQSQQAAVSRQLEDFRSKRL
                     MGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEETQHTISTDEEKSAAVVT
                     AISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGE
                     PSAPDCRHLKVEADEKPGPADRKGGLGMSSRDRTVGTPGQDNPGVSLHPLSTDSVDSR
                     EPGVDTPQEPGDTPPQGPTSLEPLAPPESTEDNGETERRWKRLSGRLGRSESLRVSDR
                     RRPSRGSLGAKGRGGGRSRSDVDMDPGSATAVLGPTRRATPEPGDDGEPGRSGLELEP
                     EEPPGWRELVPPDTLLSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMADGG
                     FFPLDELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWF
                     QKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLT
                     KYPLLLQSIGQNTEESTERGKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDL
                     THLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAIEVHVLLLDDLLLLLQRQD
                     ERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYEL
                     VAQTSSERKNWCNLITETAGSLKVPAPASRLKPRPSPSSIREPLLSSSENGTGGAEMA
                     PADARTERLLNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTEEDSGAGPPRDGDGV
                     PGGRAPGPVHTQEIEENLLSLEVAIRQLEELEEEFCRLRPLLSQLGGTLSPNLAAPER
                     SAQTGLS"
     exon            150..230
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            231..344
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            345..443
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            444..486
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            487..733
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            734..763
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            764..863
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            864..960
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            961..996
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1134
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1240
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1252
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1420
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1566
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1713
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1796
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1922
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2037
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2138
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2216
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2292
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2293..2460
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2548
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2635
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2636..2703
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2790
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2954
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3054
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3407
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3387..3392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="hexamer: AATATA"
     polyA_site      3407
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="major polyA site"
ORIGIN      
        1 gacttcctgc ccgccagagc caggaagccg gagtcgggac accgggcccg ggatccccga
       61 gcccgacctc gggcgcctcg ccggtcacct ccccgcgcgg acaccagccc ggcggagtcc
      121 tggagatggg agaagtcgcc ggaggggcgg ccccagggcc tccccggtct ggcctggtgt
      181 ccatcatcat cggggcggag gatgaggatt ttgagaacga gctggaggcg aactcagaag
      241 atcaaaacag ccagttccag agcctagagc aagtgaagcg ccgccctgcc cacctcatgg
      301 ccctcctgca gcatgtggcc ctgcagttcg agccaggacc actgctctgc tgcctgcatg
      361 cagacatgct gagctctctg ggccccaaag aagccaagaa ggccttcctt gacttctatc
      421 acagtttcct ggagaagact gcggttctac gggtgccggt ccctcccagt gtcgcttttg
      481 aacttgatcg tactcgacct gatctgatct ctgaggatgt ccagaggcgg ttcatacaag
      541 aggtggtgca gagccagcag gcagccgtga gccgtcagct agaggacttc cgctccaagc
      601 ggctcatggg catgacgccc tgggagcagg aactgagcct gctggagccc tggattggga
      661 aagaccgagg caactatgag gcccgggagc ggcatgttgc ggagcggctg ctgtcccacc
      721 tggaggagac ccagcatacc atctctacag atgaagagaa aagtgctgct gtggtcactg
      781 ccatcagcct gtatatgcgc caccttggag tccggaccaa gagtggggac aagaagtcgg
      841 gaaggaactt cttccggaaa aaggtgatgg ggaatcggag gtcagacgaa cccccaaaga
      901 caaagaaagg gctgagcagt atcctagatc ctgcacgttg gaaccgggga gagccatccg
      961 ctccagattg tcgacatcta aaggtcgagg ctgatgagaa gccaggccct gcagaccgga
     1021 agggaggcct gggtatgtct tctcgggaca ggactgttgg gactcctgga caggacaacc
     1081 caggagtctc cctgcaccct ctgtctacag acagcgtcga ctcccgggaa ccaggcgtgg
     1141 ataccccgca ggagccaggg gatacacccc cacagggccc taccagcctg gagcccctgg
     1201 cgcccccaga gagcacagag gacaatggcg agactgagag aaggtggaag aggctatcag
     1261 ggcgtctagg gcgctcagag agcctgcggg tgagtgaccg ccgccggcct tcccggggca
     1321 gcctcggggc taagggccgg ggtgggggcc gttcccggag cgacgtggac atggacccgg
     1381 gctccgccac ggccgtgctt ggccccaccc gacgagccac ccctgagccc ggagatgatg
     1441 gggagccagg acggtcaggc ctggaactgg aaccagaaga acctcctggg tggagggaac
     1501 tcgtgccccc agacaccctg ctcagtctgc ccaagagcca agtgaagcgg caagaggtca
     1561 tcagcgagct gctcgtgact gaggcagctc acgtgcgcat gctacgggta ctgcatgacc
     1621 tcttctacca gcccatggcg gatggaggct tcttccctct ggacgagctg cagaacatct
     1681 tcccgagcct ggatgagctc atcgaggtgc actccctgtt cctcgatcgc ttgatgaagc
     1741 ggagacaaga gagtggctac ctcattgagg agatcggcga tgtgctactg gcccggttcg
     1801 atggtgctga gggctcatgg ttccagaaga tctcctcccg cttctgcagc cgccagtcgt
     1861 tcgctctaga gcagctcaaa gccaagcagc gcaaggagcc tcggttctgt gcctttgtgc
     1921 aggaagctga gagccgcccg agatgccggc gcctacagtt aaaggacatg atccccactg
     1981 agatgcagcg actgaccaag tacccactgc tgctacagag catcgggcag aacacagagg
     2041 agtctacaga acgagggaaa gtggagcttg cagctgagtg ctgccgggag attctgcacc
     2101 atgtcaatca agccgtccgt gacatggagg acctgctgcg gctcaaggat taccagcggc
     2161 gcctggactt gactcaccta cggcagagca gtgaccctat gctgagcgag ttcaagaacc
     2221 tggacatcac taagaagaag ttggtccatg aaggccccct cacgtggcga gtgaccaaag
     2281 acaaagctat agaagtgcac gtgctcttgc tggacgacct gctgctgctg ctccagcgcc
     2341 aggacgagag gctgctgctc aagtcccaca gccggacgct gacacctacc cccgatggca
     2401 agaccatgct gcggccggtg ctccggctca cctctgccat gacccgagag gtggccactg
     2461 atcacaaagc tttctacgtc atttttacct gggaccagga ggcccagata tatgagctgg
     2521 tggcacagac atcttcggaa cgcaaaaact ggtgtaacct catcactgag actgctggat
     2581 ccctgaaggt ccctgcccct gcctcccgcc tcaaaccccg gcccagccca agcagcatcc
     2641 gagaacccct gctcagcagc tctgagaatg gcactggagg cgcagagatg gctccagctg
     2701 atgccaggac agagcggctc ctcaatgacc tcctgccctt ctgcagacca ggcccagagg
     2761 gccagcttgc tgccacagcc cttcagaaag tactgtccct gaagcagatc ctgctaagca
     2821 ctgaggaaga cagtggagcg gggcctcccc gcgatgggga tggggtgcct ggtggtaggg
     2881 cccccggccc agtgcacacc caggagattg aggaaaactt gcttagctta gaggtggcca
     2941 tcagacaact ggaggagttg gaagaggaat tttgtcgcct aagacccctc ctgtcccagc
     3001 ttggggggac tctgtccccc aacctggctg cacctgaacg ctctgctcag acaggccttt
     3061 catgaagaga agagtggggg agaggatgca ggggggccca cccccacccc cacagctgcc
     3121 acagcatctc acaccccaga ggcctggagc agagggagat tggctgaact tgatagcaga
     3181 cctgctcggg tccctgcctc ccactctggc ctctgccttt ctccctcctg ccttctgctt
     3241 ggggaactca gggcttcact cccagagcac ccccttaatc cccacttttc aggccgtctc
     3301 agtattgcct atggggggtc accctccttc ccctacccca agtgcctttg ctatgttttt
     3361 atatcctgga ctggaggttt atttttaata tatattatct aaggaaa
//
