LOCUS       NM_001164628            1519 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rho GTPase activating protein 8 (Arhgap8), transcript
            variant 2, mRNA.
ACCESSION   NM_001164628
VERSION     NM_001164628.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL611987.16, AK086816.1 and AL513352.12.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 4. Variants 1, 2, 3 and 4 encode the same
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK086816.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL611987.16        48458-48484
            28-1518             AK086816.1         2-1492
            1519-1519           AL513352.12        20682-20682
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.99 cM"
     gene            1..1519
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /note="Rho GTPase activating protein 8"
                     /db_xref="GeneID:73167"
                     /db_xref="MGI:MGI:1920417"
     exon            1..56
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            57..190
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1389
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /note="rho-type GTPase-activating protein 8"
                     /codon_start=1
                     /product="rho GTPase-activating protein 8"
                     /protein_id="NP_001158100.1"
                     /db_xref="CCDS:CCDS27712.1"
                     /db_xref="GeneID:73167"
                     /db_xref="MGI:MGI:1920417"
                     /translation="MAGLDPTLSTSHPFYDVARHGILQVAGDDRQGRRIFTFSCCRLP
                     PLHQLNHQRLLEYLKYTLDQHVENDYTIVYFHYGLSSQNKPSLGWLQNTYKEFDRKYK
                     KNLKALYVVHPTSLIKALWNIFKPLISHKFGKKVTYCSNLRELREHLQCDQLLIPPEV
                     VRYDEKLQNLHKGQPPPPTKTPPPRPPLPTQQFGVSLQYLRDKNQGELIPPVLRWTVT
                     YLREKGLRTEGLFRRSASAQTVRQVQRLYDQGKPVNFDDYGDMHLPAVILKTFLRELP
                     QPLLTFQAYEQILGITSVESSLRVTHCRLILRSLPEHNYAVLRYLMGFLHEVSLESIS
                     NKMNSSNLACVFGLNLIWPSQGVASLSALVPLNLFTELLIEYYDKVFSCQEAPGEHTR
                     DTVEVQQAGPVTKEFMKTGTPRASPYLSRLRIS"
     exon            191..278
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            279..410
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            411..497
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            498..596
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            597..707
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            708..781
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            782..859
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            860..988
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1092
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1519
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1496..1501
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1519
                     /gene="Arhgap8"
                     /gene_synonym="3110043J09Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggggaggggc ctgtccgggg acgggccagg tgtgtggctg gcgttgcaga gaccagagac
       61 aaggaagcag cagtcaccct gggggccaca gaagggccct cagtggcgtc catggctggc
      121 ctggacccca cactgagcac aagtcaccca ttctatgatg tggccagaca cggcatcctg
      181 caggtggcag gggatgaccg ccaggggaga cgcatcttca ctttcagctg ctgccggctg
      241 ccacccttgc accagctcaa ccaccagcgc ctgctggagt atctgaagta cacactggac
      301 cagcatgtgg agaacgacta tactattgtc tactttcact atggcctcag cagccagaac
      361 aaaccatccc taggctggct ccagaacacc tacaaggagt ttgaccggaa gtacaagaag
      421 aacctgaagg cactgtacgt tgtacacccc accagcctca tcaaagcact gtggaacatc
      481 ttcaagcccc tcatcagtca caagtttggg aaaaaagtca cctactgcag caacctgcgt
      541 gagctccgag aacaccttca gtgcgaccag ctgctcatcc ccccggaagt agtgcggtat
      601 gacgagaaac tgcaaaacct gcacaaaggt caaccgcccc cgcccaccaa gacaccgcca
      661 cctcggccgc ctctgcccac ccagcagttt ggcgtcagcc tgcaatacct cagagacaaa
      721 aatcaaggtg aactcatccc cccggtgctg cgttggacgg tgacatatct gagagaaaaa
      781 ggactgcgca ctgagggcct gttccggaga tcggccagcg cccagactgt ccgtcaggtg
      841 cagcggctat atgatcaagg gaagcccgtg aactttgatg attatggaga catgcacctc
      901 ccagccgtga tcctgaagac atttctccga gagctgcccc agccactgct gaccttccaa
      961 gcctacgagc agattctcgg gatcaccagt gtggagagca gccttcgagt gacccactgc
     1021 cgcctgatcc tgcggagcct cccagaacac aactatgccg tactccgcta cctcatgggc
     1081 ttcctgcatg aagtgtctct ggagagcatt tcaaacaaga tgaacagctc taacctggcg
     1141 tgcgtgtttg gattgaactt gatctggcca tcccaggggg tggcttccct gagcgccctg
     1201 gtacctctga acttgttcac agagctgctg atagagtact atgacaaagt cttcagttgc
     1261 caggaggccc ctggggagca cacccgggat actgtggaag tacagcaggc tggccctgtt
     1321 accaaagaat ttatgaagac gggcactccc cgggcctcac catacctgtc ccgcctccgt
     1381 atctcctgac tagcttgaga cacctcaaga tggagactgg agatgccccc ttccaatcta
     1441 ggcatctgtg tacctgtttg tcttatgaac ttactatctt taagaagaca caggaattaa
     1501 atgaacccag aacttccta
//
