LOCUS       NM_001408223            4032 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Rho GTPase activating protein 44 (Arhgap44),
            transcript variant 3, mRNA.
ACCESSION   NM_001408223 XM_006532871
VERSION     NM_001408223.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Zhang,J., Gong,L., Zhu,H., Sun,W., Tian,J., Zhang,Y., Liu,Q.,
            Li,X., Zhang,F., Wang,S., Zhu,S., Ding,D., Zhang,W. and Yang,C.
  TITLE     RICH2 decreases the mitochondrial number and affects mitochondrial
            localization in diffuse low-grade glioma-related epilepsy
  JOURNAL   Neurobiol Dis 188, 106344 (2023)
   PUBMED   37926169
  REMARK    GeneRIF: RICH2 decreases the mitochondrial number and affects
            mitochondrial localization in diffuse low-grade glioma-related
            epilepsy.
REFERENCE   3  (bases 1 to 4032)
  AUTHORS   Sarowar,T., Grabrucker,S., Boeckers,T.M. and Grabrucker,A.M.
  TITLE     Object Phobia and Altered RhoA Signaling in Amygdala of Mice
            Lacking RICH2
  JOURNAL   Front Mol Neurosci 10, 180 (2017)
   PUBMED   28642683
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4032)
  AUTHORS   Sarowar,T., Grabrucker,S., Fohr,K., Mangus,K., Eckert,M.,
            Bockmann,J., Boeckers,T.M. and Grabrucker,A.M.
  TITLE     Enlarged dendritic spines and pronounced neophobia in mice lacking
            the PSD protein RICH2
  JOURNAL   Mol Brain 9, 28 (2016)
   PUBMED   26969129
  REMARK    GeneRIF: RICH2 knockout animals display a selective and highly
            significant fear of novel objects and increased stereotypic
            behavior as well as impairment of motor learning.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4032)
  AUTHORS   Raynaud,F., Moutin,E., Schmidt,S., Dahl,J., Bertaso,F.,
            Boeckers,T.M., Homburger,V. and Fagni,L.
  TITLE     Rho-GTPase-activating protein interacting with Cdc-42-interacting
            protein 4 homolog 2 (Rich2): a new Ras-related C3 botulinum toxin
            substrate 1 (Rac1) GTPase-activating protein that controls
            dendritic spine morphogenesis
  JOURNAL   J Biol Chem 289 (5), 2600-2609 (2014)
   PUBMED   24352656
  REMARK    GeneRIF: These results suggested that Rich2 controls dendritic
            spine morphogenesis and function via inhibition of Rac1.
REFERENCE   6  (bases 1 to 4032)
  AUTHORS   Raynaud,F., Janossy,A., Dahl,J., Bertaso,F., Perroy,J.,
            Varrault,A., Vidal,M., Worley,P.F., Boeckers,T.M., Bockaert,J.,
            Marin,P., Fagni,L. and Homburger,V.
  TITLE     Shank3-Rich2 interaction regulates AMPA receptor recycling and
            synaptic long-term potentiation
  JOURNAL   J Neurosci 33 (23), 9699-9715 (2013)
   PUBMED   23739967
  REMARK    GeneRIF: Knock-down of Rich2 or disruption of Rich2-Shank3 complex,
            inhibits dendritic spine enlargement.
REFERENCE   7  (bases 1 to 4032)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 4032)
  AUTHORS   Richnau,N. and Aspenstrom,P.
  TITLE     Rich, a rho GTPase-activating protein domain-containing protein
            involved in signaling by Cdc42 and Rac1
  JOURNAL   J Biol Chem 276 (37), 35060-35070 (2001)
   PUBMED   11431473
  REMARK    GeneRIF: Shows that the RhoGAP domain of the human RICH2 ortholog
            can catalyze GTP hydrolysis of both Rac1 and Cdc42, but not of
            RhoA.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL663045.7.
            
            On Jun 14, 2022 this sequence version replaced XM_006532871.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363660.1294969.1,
                                           ERR3363658.1864422.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-368               AL663045.7         186266-186633       c
            369-408             AL663045.7         98851-98890         c
            409-513             AL663045.7         98117-98221         c
            514-590             AL663045.7         90760-90836         c
            591-702             AL663045.7         83504-83615         c
            703-779             AL663045.7         80977-81053         c
            780-897             AL663045.7         76779-76896         c
            898-966             AL663045.7         65122-65190         c
            967-1048            AL663045.7         62831-62912         c
            1049-1176           AL663045.7         62296-62423         c
            1177-1288           AL663045.7         55764-55875         c
            1289-1370           AL663045.7         55331-55412         c
            1371-1451           AL663045.7         50484-50564         c
            1452-1565           AL663045.7         47871-47984         c
            1566-1657           AL663045.7         46806-46897         c
            1658-1838           AL663045.7         45621-45801         c
            1839-1856           AL663045.7         36587-36604         c
            1857-2084           AL663045.7         35551-35778         c
            2085-2242           AL663045.7         33562-33719         c
            2243-2620           AL663045.7         31743-32120         c
            2621-4032           AL663045.7         25719-27130         c
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 40.42 cM"
     gene            1..4032
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /note="Rho GTPase activating protein 44"
                     /db_xref="GeneID:216831"
                     /db_xref="MGI:MGI:2144423"
     exon            1..368
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     CDS             316..2760
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     Rho-type GTPase-activating protein RICH2; RhoGAP
                     interacting with CIP4 homologs protein 2; Rho
                     GTPase-activating protein RICH2; rho GTPase-activating
                     protein 44"
                     /codon_start=1
                     /product="rho GTPase-activating protein 44 isoform 3"
                     /protein_id="NP_001395152.1"
                     /db_xref="GeneID:216831"
                     /db_xref="MGI:MGI:2144423"
                     /translation="MKKQFNRMRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSH
                     STHKKLTACLQGQQGAEADKRSKKLPLTTLAQCLVEGSAILGDDTLLGKMLKLCGETE
                     DKLAQELIHFELQVERDVIEPLFLLAEVEIPNIQKQRKHLAKLVLDMDSSRTRWQQTS
                     KSSGLSSSLQPAGAKADALREEMEEAANRVEICRDQLSADMYSFVAKEIDYANYFQTL
                     IEVQAEYHRKSLTLLQAVLPQIKAQQEAWVEKPSFGKPLEEHLMISGREIAFPIEACV
                     TMLLECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLREL
                     PEPLMTFELYDEWIQASNIQEQDKRLQALWNACEKLPKANHNNIKYLIKFLSKLSEYQ
                     DVNKMTPSNMAIVLGPNLLWPQSEGNITEMMTTVSLQIVGIIEPIIQHADWFFPGEIE
                     FNLTGSYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARRPLSVATDNMMLEFYKKDG
                     LRKIQSMGVRVMDTSWVARRGSSAGRKASCAPPSMQPPAPPSELAAPLPSPLPEQVPD
                     SPATPAPALSPSGASLQPTPERPSVSKSKELSPGSGQKGSPGSIQGTPCPGTQLGPQP
                     AASPSQLPADQSPHTLRKVSKKVAPIPPKVPFVQPGTVSDQPVGQPSPVSLSPTPPST
                     PSPYGLSYPPGYSMASGQLSPASAPPLASPSVFTSTLAKSRPTPKPRQRPTLPPPQPP
                     SVSLSASSPQSTEHPMLDGMSPGESMSTDLVHFDVPSIHIELGSTLRLSPLEHARRHS
                     ATDKRDSEEESESTAL"
     exon            369..408
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            409..513
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            514..590
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            591..702
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            703..779
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            780..897
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            898..966
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1048
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1176
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1288
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1370
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1451
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1565
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1657
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1838
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1856
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2084
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2242
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2620
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..4032
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4008..4013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /note="hexamer: ATTAAA"
     polyA_site      4032
                     /gene="Arhgap44"
                     /gene_synonym="6330543G20; Rich2"
                     /note="major polyA site"
ORIGIN      
        1 acccggccag ctggctagcc agcctgcgga gactccccgg ttcgctcgcc gggctggggc
       61 tgggggctag ctacagctgg ggctgaccgc cgggcgcctt gggcggcccc gggcgtggca
      121 cagagaagga tgcggcgggc ggactaggcg gaggtgctct cgggaggctg ggcgcgggcc
      181 aggcggctcc ccaggctccg cagtgccgcc gccgtcgccc gggaggctgg gcgcgggagc
      241 catgtaaccc tgcggtgggc tccgggcagc tcagtccttc cccagctccc gggctagcgc
      301 ggcagcgggg ccacgatgaa gaagcagttt aaccgcatgc gccagttggc caaccagacg
      361 gtgggcaggg cagaaaagac tgaagttctg agcgaagacc ttctccaggt ggagaagcgc
      421 ctagagctgg tgaagcaggt gtcccacagc acacacaaga agctcaccgc atgtctgcag
      481 ggtcagcagg gagcagaggc cgacaagcga tcaaaaaagt tgcctctgac gactctggcg
      541 cagtgcttgg tggaggggtc cgcgatcctg ggcgatgaca cgcttcttgg gaagatgctg
      601 aagctctgcg gagagacgga ggacaagcta gctcaggagc tgatccactt tgagctgcaa
      661 gtggagaggg atgtgatcga gcccctgttc ctgctcgctg aggtggaaat tccaaatatt
      721 caaaagcaga ggaaacattt agccaagctg gtgcttgaca tggactcttc ccggaccagg
      781 tggcagcaga cttccaagtc ttcagggctt tccagcagcc ttcagcctgc gggtgccaaa
      841 gccgatgccc tcagggagga gatggaggag gctgccaaca gagtggagat ttgccgggac
      901 cagctttctg ctgacatgta cagttttgtg gccaaagaaa ttgactatgc aaactacttt
      961 caaacgctaa tagaagtgca agctgagtac cacaggaagt cgctgacact gctgcaggct
     1021 gtgttgcctc agatcaaagc ccagcaggag gcctgggtgg agaagccttc ctttgggaag
     1081 cccctggagg agcacctgat gatcagtggc cgagagatcg ccttccctat cgaggcgtgt
     1141 gtgaccatgc tgctggagtg tgggatgcag gaggagggcc tcttccgggt agccccctca
     1201 gcctccaagc tgaagaagct gaaagctgct ctggactgct gtgtggtgga tgtccaggag
     1261 tactcggctg acccccacgc catagcaggg gctttgaagt cttatctccg agagttgcca
     1321 gagcctctta tgacctttga actctatgac gaatggatcc aggcttctaa tatccaggag
     1381 caagacaaga gacttcaggc tctctggaat gcttgtgaga agttgcccaa ggccaaccat
     1441 aacaacatca aatacttgat caaattttta tcgaaactgt ctgaatatca ggatgtaaac
     1501 aagatgactc ccagtaacat ggcaattgtc ctgggaccca atctcctttg gccacagtca
     1561 gaggggaaca tcaccgagat gatgaccaca gtttccctgc agatcgttgg catcatcgag
     1621 cccattatcc agcacgcaga ctggttcttc cctggagaga tagagttcaa cctcacaggc
     1681 agttacggga gtccagtcca cgtgaaccac aatgccaact acagctcaat gccctcccca
     1741 gacatggacc ctgctgaccg gcgccagcca gagcaggccc gccggcccct cagtgttgcc
     1801 acagacaaca tgatgctgga gttttacaag aaggatggcc ttaggaaaat ccaaagcatg
     1861 ggtgtgaggg tcatggacac atcttgggtg gcacgaagag gctcctctgc tggtcggaaa
     1921 gcatcctgcg ccccgccttc catgcagcct cctgccccac cctccgagct ggctgcaccc
     1981 ttgccttcac ccctgccgga gcaggtccca gacagccctg ccactccagc tcctgcgctc
     2041 tctccatcag gtgccagcct gcagcccacc cccgagcgcc ccagtgtgtc taaaagcaag
     2101 gaactgtcgc cagggtctgg gcagaaagga agtccaggtt ccatccaggg gacaccctgc
     2161 ccagggacgc aactggggcc acagccggct gccagcccca gccagcttcc tgcagaccag
     2221 agtcctcaca ccctccggaa agtttcaaag aaggtggcac caattcctcc caaggtcccc
     2281 ttcgtgcagc cagggactgt ttcagatcag cccgttggcc agccatcccc agtcagcctg
     2341 tctcctactc caccaagcac cccatccccc tatggactga gctaccctcc aggctactcc
     2401 atggcctctg gccagctttc tcctgcctca gctcctcccc tggcctctcc ctctgtcttt
     2461 acaagcactt tagccaaatc tcggcccacc cctaagcctc gacagaggcc caccctgccg
     2521 cctcctcagc ctcccagtgt cagcctgtcg gcttcaagtc cacagtccac agagcacccc
     2581 atgttagacg gcatgtcgcc gggggaaagc atgtctacag atctcgtcca ctttgatgtc
     2641 ccctccatcc acatagagct ggggtcaacg ctccgcctga gccccctgga acatgcacgg
     2701 cggcactcgg caacagacaa gagggactcg gaagaggagt ctgagagtac agccctttga
     2761 tgtggcccaa cctacacgtg tacatacgtc tgtgggccct gagacaccac ccagggcagt
     2821 gctggctagc ctgccaggga cttgccatca gctctttgct gtcatgtcca acaccagggt
     2881 ggagttgggc ggaggaaaag agttctcctg tccatgtggc cagtgttggt ggtgcagatt
     2941 ctgtttgttc agtctgggtg atgattccag cttttttttt ttttttttta ccctttgcct
     3001 tttgactttg tgcctatttt gatccatctt tggcctctat ggcaagcagc atccaactct
     3061 ccactcaagg gcttgtcagg atggcctttg caggatgagg gtgcagaaga gtccccttac
     3121 tgccttgact gggacatcag gtcctacagc atcatcataa gggccagaga agaagcaaga
     3181 caggaggaga ccctttcact agggcaagag atgtcctggg gccaaccaga aaaacctttc
     3241 aggccaccct tagtcacagc tccaaagtac tcagcttcag acatccactt ctgacagctt
     3301 tactttcccc gtgtgatgac ggcttcctct actccacgct gacaatctga actgaggatt
     3361 tgaacataag tgaccctacg taggtctcca tgttcccaaa cacattaggg atcttagagc
     3421 actttcgcat tcaggcaccc cgcttagtag gtgtgtgggg cctgactctc ctcttctgga
     3481 ggttacagct gactagcaat aattctgttt cccaaagcag gtggagaggg tggtactggg
     3541 cacctagccc acagcgtggc ctgcgtggct caccagcaga ccctgctcat ccttctcacc
     3601 acgtcccatg cagccctggg gcaagggcgc aggaagcctg ttcatacttt gggattaagg
     3661 gaataaaaca gatctgggcc atgcatgcac agtcagttta aagtttgttc ctctgaaaat
     3721 agatatacct atacatgata tatttgtata tatgaaatct atatttgttt aatttgagcc
     3781 attcaatcta aacctatgta caggtgtaca atggaaaatt ctaaatgctt agttattttt
     3841 cccagcagtg tgaactcacc cgtctctgag agtgggatct gcagactcga ccttacagct
     3901 ttgctcactt cctggcaagg gcagtccagt ccccaaattt gtaacggagc ctgggggttg
     3961 aaggttatct ttacatatat gtacaaactg ttgtcaagag taatgttatt aaaatagatt
     4021 tattaaccct ga
//
