LOCUS       NM_138630               3321 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rho GTPase activating protein 4 (Arhgap4), transcript
            variant 1, mRNA.
ACCESSION   NM_138630
VERSION     NM_138630.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Bai,Y., Luo,Y., Liu,S., Zhang,L., Shen,K., Dong,Y., Walls,C.D.,
            Quilliam,L.A., Wells,C.D., Cao,Y. and Zhang,Z.Y.
  TITLE     PRL-1 protein promotes ERK1/2 and RhoA protein activation through a
            non-canonical interaction with the Src homology 3 domain of p115
            Rho GTPase-activating protein
  JOURNAL   J Biol Chem 286 (49), 42316-42324 (2011)
   PUBMED   22009749
  REMARK    GeneRIF: PRL-1 binding to p115 RhoGAP provides a coordinated
            mechanism underlying ERK1/2 and RhoA activation
REFERENCE   3  (bases 1 to 3321)
  AUTHORS   Chen,X.W., Leto,D., Xiong,T., Yu,G., Cheng,A., Decker,S. and
            Saltiel,A.R.
  TITLE     A Ral GAP complex links PI 3-kinase/Akt signaling to RalA
            activation in insulin action
  JOURNAL   Mol Biol Cell 22 (1), 141-152 (2011)
   PUBMED   21148297
REFERENCE   4  (bases 1 to 3321)
  AUTHORS   Christerson,L.B., Gallagher,E., Vanderbilt,C.A., Whitehurst,A.W.,
            Wells,C., Kazempour,R., Sternweis,P.C. and Cobb,M.H.
  TITLE     p115 Rho GTPase activating protein interacts with MEKK1
  JOURNAL   J Cell Physiol 192 (2), 200-208 (2002)
   PUBMED   12115726
  REMARK    GeneRIF: The p115 Rho GAP binds to MEKK1 in vitro and in intact
            cells. Expression of p115 Rho GAP reduces MEKK1-induced signaling
            to AP-1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672002.14, AK028789.1 and AK040687.1.
            
            On Jul 2, 2009 this sequence version replaced NM_138630.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK157294.1, AF443826.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AL672002.14        29504-29639         c
            137-1149            AK028789.1         484-1496
            1150-1194           AL672002.14        19018-19062         c
            1195-3312           AK040687.1         1-2118
            3313-3321           AL672002.14        12693-12701         c
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 37.45 cM"
     gene            1..3321
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /note="Rho GTPase activating protein 4"
                     /db_xref="GeneID:171207"
                     /db_xref="MGI:MGI:2159577"
     exon            1..136
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2937
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GTPase-activating protein p115"
                     /codon_start=1
                     /product="rho GTPase-activating protein 4 isoform 1"
                     /protein_id="NP_619536.2"
                     /db_xref="CCDS:CCDS41014.1"
                     /db_xref="GeneID:171207"
                     /db_xref="MGI:MGI:2159577"
                     /translation="MAAHGKLRRERGLQPEYDAQVKEMRWQLSEQLHCLELQGELRRD
                     LLQELAEFMRRRAEVELEYSRGLDKLAERFTSRSGRLGGSSQEQQSFRKEPTLLSPLH
                     CWAVLLEHTRQQSRESAALSEVLAGPLAQRLSHISEDVGRIVKKSKDLVQQLQAELLE
                     VVSELQTTKKMYHVYHSESMNAETKLREAERQEEKRSGRSALSTTTTTSATTTTTTTT
                     VETGPHRKSSLKKGGRLVEKRQAKYLEHKLKCTKARNEYLLSLASVNAAISNYYLHDI
                     LDLMDCCDTGFHLALEQALRSYTAAESRTQTSQMQGLGSLEEALEALDPPGDKAKVLE
                     VHAMAFCPPLRFEYQPHEDDEVAEVLIEMELWDEILPRAQNIQSRLDQKTIETEEVNK
                     TLKATLKALLEAVASEDGDILDSLQASPSTESLKSTSSDPGTRQTGRRRNQQQETETF
                     YITKLQEYLSGRSILSKLQAKHEKLQEAIQQGNKEKQETSRTQCTERKFHKSHPPHPR
                     FQYNQRLFGGDLEKFIQSSGQPVPLVVESCIRFINLNGLQHEGIFRVSGAQARISEIR
                     DAFERGEDPLEKGCTVHDLDSVAGVLKLYFRSLEPPLFPLDMFNELLASAELEVVGER
                     VEPVSHLLFKLPRPVLVVLRYLFTFLNHLAQYSDENMMDSYNLAVCFGPTLLPVPAGQ
                     DPVALQGRVNQLVQTLILQPARIFPPPAMLPGPIYEKCMAPPSASCLGDGQLESLIGE
                     PELELKTGTTAQEDDQEGVLEAVACFAYTGRTAQELTFQRGDVLRLYARASSDWWRGE
                     HAGVQGLIPHKYITLCEGDEKQMAGSGLQAMGESVSHPEGFLTLEFANRLELGTPPEA
                     LGPSRHRRPCLVPTSPEGHVEMDKAVAQNMNSVFKELLGKTAVRQGHGLASTASPSLG
                     TLTPLACSSISKNKVFSRGPGAPISPSTSHPQGPDSTRKPV"
     exon            137..344
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..507
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            508..570
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            571..783
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            784..912
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1134
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1236
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1428
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1513
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1541
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1644
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1705
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1783
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1918
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2024
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2258
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2332
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2516
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2609
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2709
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..3321
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3120..3125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /note="hexamer: CATAAA"
     polyA_site      3141
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /note="major polyA site"
     regulatory      3289..3294
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
                     /note="hexamer: AATACA"
     polyA_site      3315
                     /gene="Arhgap4"
                     /gene_synonym="A130009C12Rik; A530015A20Rik; c1;
                     mKIAA0131; Rgc1"
ORIGIN      
        1 gcacttggcg gtctcgcctc gcttccgcgt gggagcattg ggactcgaag tcgcgaacgg
       61 ccttgggcca tggcggcgca cgggaagttg cggcgggagc gggggctaca acctgagtat
      121 gacgcacagg tcaaagagat gcgctggcag ttgagtgagc agcttcattg cctggagctt
      181 cagggagagc ttcggcggga tttgctgcag gagctagctg agttcatgcg gcgccgggcg
      241 gaggtagaac tggagtattc tcggggcctg gacaagttgg ctgaacgctt taccagccgc
      301 agtggtcgcc ttgggggcag cagccaggag cagcaaagct tccggaagga gccgactctc
      361 ctgtcaccct tgcactgctg ggctgtgctg ctggaacaca ctcggcagca gagtcgggaa
      421 agtgctgccc tcagtgaggt gctggctggg cccctggcac agcgcctaag tcacatcagc
      481 gaagatgtgg gacgtattgt caaaaagagc aaggacctgg tgcagcagtt gcaggctgag
      541 ctcctggaag tggtctcaga actccagaca accaagaaga tgtaccatgt gtaccactca
      601 gagagcatga atgctgagac taaacttcgg gaagcggagc ggcaagagga gaagcggtca
      661 ggcaggagtg ccttatccac caccactact acttctgcca ccaccaccac tactaccacc
      721 accgttgaga cagggcccca ccgaaagagc tctctcaaga aaggagggcg gctagtggag
      781 aagcgtcagg ccaagtactt ggagcataaa cttaagtgca ccaaggcccg aaatgagtac
      841 ctgctcagcc tggccagtgt caatgccgcc atcagcaact actacttaca cgatatcttg
      901 gatctcatgg attgctgtga cacaggtttc catttggccc tggagcaggc cctccggagc
      961 tacacagctg ctgagagccg tacccaaacc tcccagatgc agggcttagg cagcctggaa
     1021 gaggctctgg aggctttgga tcctccaggg gacaaggcca aggtgcttga ggttcatgcc
     1081 atggccttct gtccccctct acgttttgaa taccagcccc atgaagatga tgaggtggcc
     1141 gaggtcctga ttgagatgga actgtgggat gagattttgc ccagagctca aaacattcag
     1201 agtcgcctgg accaaaagac catcgagaca gaagaggtga ataagacact gaaggcaaca
     1261 cttaaggctc tgctagaggc ggtggcatca gaggatgggg acatactgga ctctttgcag
     1321 gccagcccct ccactgagtc cctcaagtct acaagctcgg acccaggcac ccgacagaca
     1381 ggccgtagac ggaaccagca gcaggagaca gaaaccttct atattacgaa gctgcaggaa
     1441 tatctgagtg gccggagcat cctttccaag ctgcaggcca agcatgaaaa acttcaggaa
     1501 gccatacaac aaggtaacaa ggaaaagcaa gagacatctc ggacccagtg cacagagaga
     1561 aaattccaca agagccatcc cccccatcct agattccagt ataatcagag actttttgga
     1621 ggtgatctgg agaagtttat ccagagctca ggccaacctg tgccccttgt ggtggagagc
     1681 tgtattcgct tcattaatct caatggcctg caacatgaag gcattttccg ggtatcaggt
     1741 gcccaggcca ggatctcaga gatccgagat gcctttgaaa gaggggagga ccctctggag
     1801 aaaggctgta ctgtccatga cctagactca gtggctgggg tactaaagct gtactttcgg
     1861 agcctggagc ccccactctt ccccttggac atgtttaatg agctgctggc ttcagcagaa
     1921 ctggaagttg tgggtgagcg agtagagcct gtgagccatc tgctattcaa actacccaga
     1981 cctgtgctag tggtcctacg ctatctcttc accttcctca accacctggc ccagtacagt
     2041 gatgagaaca tgatggactc ctacaacctg gctgtatgtt ttgggcctac actgttgcct
     2101 gtgcctgctg ggcaagaccc agtagccttg caaggccgag tgaaccagct ggtccagacc
     2161 cttatcctgc aaccagcacg gattttccca cctccagcca tgctgccagg ccccatctat
     2221 gagaaatgca tggcaccacc ttctgccagc tgcctggggg atggccagtt ggagagcctc
     2281 attggggagc cagagctgga gttgaaaact gggaccacag ctcaggagga tgaccaggag
     2341 ggagttttgg aggctgtggc ctgctttgcc tacactggtc gtacggccca ggagctgaca
     2401 ttccagcgag gggatgtgct gcgattgtat gctcgggcat caagtgactg gtggcgaggg
     2461 gagcatgctg gtgtgcaggg gctcattccc cataagtaca tcacactttg tgagggggat
     2521 gagaagcaga tggctggttc aggactacag gccatggggg aatctgtgag ccatccagag
     2581 ggcttcctga cgttggagtt tgccaaccgg ttggaactag gcaccccacc tgaggctttg
     2641 ggcccctcta ggcacagacg accctgttta gtccctacct cccctgaagg acatgtggaa
     2701 atggataagg ctgtggcaca gaacatgaat tctgtgttta aggagctctt gggaaagact
     2761 gctgtccgcc agggccatgg gttagcatct acagcctccc cgagtctagg aactctgacg
     2821 cccctggcct gcagcagcat cagcaaaaac aaagtcttct cccggggacc tggggctcca
     2881 atttccccct caacctccca tccccagggt ccagattcaa ctcgcaagcc agtctgaggc
     2941 agcttacctc cagcagccac caccaagccc accattccac ataggcctat tgcttcacct
     3001 cagccctctt ctaggtctct tgggaggtag caaaggaagg aagctggaga tgagctcctc
     3061 ttatgccttt gcttggaagc cttgggctgt ggctgtgaaa tatggaagtg gcagtaattc
     3121 ataaagaccc catgtgctgg ttgctgttgt aatagcctgt gctgtttggg gggcgctgaa
     3181 caggtagggt gcagggcaac tcccagagcc tgcactgcta gagaccagat gccaagacag
     3241 aaggttgaca tggagctgtt tattttcacc tggggacctg ctctcaaaaa tacagtcttg
     3301 gttgcttata tcacagattc c
//
