LOCUS       NM_172964               5317 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus Rho GTPase activating protein 28 (Arhgap28),
            transcript variant 1, mRNA.
ACCESSION   NM_172964
VERSION     NM_172964.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Santos,R., Kawauchi,S., Jacobs,R.E., Lopez-Burks,M.E., Choi,H.,
            Wikenheiser,J., Hallgrimsson,B., Jamniczky,H.A., Fraser,S.E.,
            Lander,A.D. and Calof,A.L.
  TITLE     Conditional Creation and Rescue of Nipbl-Deficiency in Mice Reveals
            Multiple Determinants of Risk for Congenital Heart Defects
  JOURNAL   PLoS Biol 14 (9), e2000197 (2016)
   PUBMED   27606604
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5317)
  AUTHORS   Yeung,C.Y., Taylor,S.H., Garva,R., Holmes,D.F., Zeef,L.A.,
            Soininen,R., Boot-Handford,R.P. and Kadler,K.E.
  TITLE     Arhgap28 is a RhoGAP that inactivates RhoA and downregulates stress
            fibers
  JOURNAL   PLoS One 9 (9), e107036 (2014)
   PUBMED   25211221
  REMARK    GeneRIF: Together, these data suggest that the regulation of Rho by
            RhoGAPs, including Arhgap28, during the assembly and development of
            mechanically strong tissues is complex and may involve multiple
            RhoGAPs.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5317)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   5  (bases 1 to 5317)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK087523.1, AK133720.1, AK141901.1 and BB372888.1.
            
            On Apr 3, 2009 this sequence version replaced NM_172964.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK141901.1, AK087523.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2361              AK087523.1         14-2374
            2362-2913           AK133720.1         2201-2752
            2914-3693           AK141901.1         2864-3643
            3694-5310           AK141901.1         3645-5261
            5311-5317           BB372888.1         209-215
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 38.9 cM"
     gene            1..5317
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /note="Rho GTPase activating protein 28"
                     /db_xref="GeneID:268970"
                     /db_xref="MGI:MGI:2147003"
     exon            1..214
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..2288
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /note="isoform 1 is encoded by transcript variant 1; rho
                     GTPase-activating protein 28; rho-type GTPase-activating
                     protein 28"
                     /codon_start=1
                     /product="rho GTPase-activating protein 28 isoform 1"
                     /protein_id="NP_766552.3"
                     /db_xref="CCDS:CCDS28950.1"
                     /db_xref="GeneID:268970"
                     /db_xref="MGI:MGI:2147003"
                     /translation="MEVEDSGGVVLTAYHSHARSQPQGAEPRCASRASHPLSRKSIPR
                     CRRINRMLSNESLHPPSFSRSNSQASVDSSASMEEFLREIESIKESSVGASQEQPPTA
                     AAAAAEVKPVDEGELEAEWLQDVGLSTLISGNEEEDGKALLSTLTRTQAAAVKKRYNT
                     YTQTLRKKNKQPVRDVRDIFGVSESPPSDSCEHATQLDGTKEEKDLPGVTKTSRPLPD
                     DASLSSTTLSNGAQDEEGGFVALQSGSVSILEAIPDIAVHTNGSADAEQSVQSTLSDD
                     DYHGKNVPAEAEELSFEVSYSEMVTEMPDRNKWKKSDIKKEDYVLTKFIIQKTRFGLT
                     ETGDLSVEDMKKIRHLSLIELTAFFDAFGIQLKRNKTERVRGRDNGIFGVPLTVLLDN
                     DRKKDPAVKVPLVLQKFFQKVEESGLESEGIFRLSGCTAKVKQYREELDARFNADKFK
                     WDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERGPDIKVQFQALHLMVMALP
                     DANRDTAQALMAFFNKVIANESKNRMNLWNISTVMAPNLFFSRSKHSDCEELLLANTA
                     THIIRLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSMKKLLRRKTLDREVSIL
                     KTSKVPQKSPSSRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKAKDILAKFQYENSH
                     GSSEHIKMQNQRLYEVGGNIGQHCLDPDAYILDVYHINPHAEWVIKP"
     exon            215..438
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            439..656
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            657..746
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            747..836
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            837..921
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1064
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1230
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1322
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1400
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1563
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1646
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1844
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1958
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2015
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2140
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2205
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..5317
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2896..2901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /note="hexamer: AATAAA"
     polyA_site      2914
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
     regulatory      5288..5293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /note="hexamer: AATAAA"
     polyA_site      5309
                     /gene="Arhgap28"
                     /gene_synonym="E130310N06"
                     /note="major polyA site"
ORIGIN      
        1 ggcgccggac gctcggggcc gctgacccgc tcccggagct agtccacgtt ggtctcggtc
       61 tttgttctgg gttagcgcgg ggacatgcgc cgctgaggat ggaggtggag gactcgggcg
      121 gcgtggtgct gaccgcctac cactcgcacg cgcgctcgca accgcagggc gctgagccac
      181 gctgcgcgtc ccgggccagc catccgctca gcagaaaatc cattcctcgc tgccgacgaa
      241 tcaacaggat gctctccaat gaatccctgc atcctccctc ctttagtcgc tccaactctc
      301 aagcctcagt agacagcagc gcatccatgg aggagttcct gcgggaaatt gagagtatca
      361 aagagagcag cgtgggggcg tcgcaggagc agccacccac tgctgctgct gctgctgctg
      421 aagtcaagcc agtggatgaa ggagagcttg aagcggagtg gctacaagat gtgggattgt
      481 caactctgat ctctggcaac gaagaggaag acggtaaagc gctcctgtct acattgactc
      541 ggacccaagc tgctgcagtg aaaaagagat acaacacata tactcagact ctgaggaaaa
      601 agaataaaca acccgtcagg gatgtcagag acatcttcgg agtcagcgaa tctcctccta
      661 gtgattcttg tgagcatgct actcagttgg acggtaccaa ggaagaaaaa gatctgccag
      721 gagttaccaa gacaagcaga cctctgccag acgatgcttc tctcagtagt accaccctat
      781 ccaatggcgc ccaggatgaa gaaggtggtt ttgtggccct acagagtggt tctgtgtcaa
      841 tacttgaggc tatcccggat attgctgttc acaccaatgg atctgcagat gctgagcagt
      901 cggttcagag cacattgagt gatgatgatt atcacgggaa gaacgttcca gcagaggctg
      961 aagagctgtc ctttgaggtc tcttactcag aaatggtgac agaaatgcct gatagaaata
     1021 aatggaagaa gtcggacatt aagaaagaag actatgtctt aactaaattt atcattcaga
     1081 aaacaagatt tgggttgacc gagacggggg acctgtctgt ggaagacatg aagaaaatcc
     1141 gccacctctc tctgattgag ctgacagcct tcttcgatgc ttttgggata caactgaaga
     1201 gaaacaaaac cgagcgagtg agaggccgag acaacgggat tttcggagtg ccacttacag
     1261 tcctcctgga caatgaccgg aagaaggacc ctgcagtgaa agttcccctg gtgttacaaa
     1321 aattttttca gaaagtcgag gaatcaggtc tggaatcgga aggaattttt cgactctcgg
     1381 gatgtactgc caaagtcaag cagtaccgcg aagaactgga tgccaggttc aatgctgaca
     1441 agttcaagtg ggacaagatg tgtcacagag aagctgcggt catgttgaaa gcattcttca
     1501 gagagctgcc cacctctctc ttccctgttg aatatatacc cgccttcatc tctctgatgg
     1561 aaagaggacc tgacatcaaa gtgcagtttc aagccttgca cctcatggtc atggccctgc
     1621 ctgatgccaa cagggacaca gcgcaggctc tgatggcatt cttcaataaa gtgatcgcca
     1681 acgaatccaa gaaccgcatg aacctgtgga acatttccac agtgatggcg cccaacttgt
     1741 tcttcagcag aagcaagcac tctgactgcg aggaactgct gctggccaac acggcgaccc
     1801 acatcatccg cctgatgctg aagtaccaga agattctgtg gaaggttcca tctttcttga
     1861 tcacccaggt cagaaggatg aatgaggcca ccatgctgtt gaagaagcag ctcccgagta
     1921 tgaagaagct gctcaggagg aagaccctgg atcgagaggt ttcaatcctc aagacctcaa
     1981 aggtaccaca aaaatcacct tcctcaagaa gaatgtctga tgtgccagaa ggtgtcataa
     2041 gggtccacgc tccacttctg tccaaagtgt ccatggccat tcagctcaac agtcagacca
     2101 aagccaaaga tatcctggcg aaatttcagt atgagaacag tcatggttca tctgaacata
     2161 ttaagatgca gaaccaaagg ttatatgaag ttggaggaaa tataggacaa cactgtttag
     2221 atcctgatgc atatatattg gatgtgtatc atataaatcc tcatgcagaa tgggtcatca
     2281 agccctagac aagtctttaa aaatatgccc aggatggttc ctgccctgta ttatatgtga
     2341 gaagatcctg catttgtggg ggaaaaaaac aaaaaactgc cctgaagtat ttgctcctgt
     2401 aatcctggtc ctcctggaga ggcgctgtgg ccagctctgg caatatgaac gacagaggag
     2461 gggctggttt catactgggc tcagcctctt agcacagttc ctcatgagac ggtgggaccc
     2521 aaaggaaagg gcagcgttgc tcattgagaa gcactgtggc tgggaagaag ctgcagttct
     2581 gaggtgaaac atacaatatt tatgctctga cttgctcctg ggttggagga ggagggcctt
     2641 cagtctaaag atctctgtgg accacgtcgc tctgtgcggc acactttgag gactggctca
     2701 caccatggtg tgagggagct ttccctgggc cctgagtact cagatgctac gttatgactc
     2761 catgggccgt ggacacagat cctcattgcc aagccctgtt tggcaaaggg catcttgtgc
     2821 gcatccagag cttccctgtg gccatgacac agccatggtg tcttccgttt ctcctctcag
     2881 ttttggtgct cagtgaataa attgcctttc tggagtcagc atggatttcc tttacagatc
     2941 aaacacattt ttttttttaa catttcacaa tgatagtcac gtccgcagcc ccaaaccaaa
     3001 agatacagaa gtggcattca tccgtaagtg ctgaaatgtg ccactaggcc gaggatttgc
     3061 aagttgtaaa agttcattgt gctctttgat cattaaccag agatgtggcc aagcattgca
     3121 gattggcact gggcttgtgg aagctggaga ctgcttcttg tcttatgtcc acatcttgtc
     3181 caaccattgt ctagacgtgc ggtgggagac attcagtcct cgtggttcta acgggaacaa
     3241 atctaaaatg tcccgaggga caggagagct ctacctttca tctcagagat tgtagttaga
     3301 gccctcattc atccgggact ccgtgcctca tcgtggacgc cacaggctgc acatctgatg
     3361 gacttaccag cagttgcaac atttaagact cgtgcggctg tgtgtcagtt caaacaaagc
     3421 cggattacag atgcggaaga actgttcctt acctgtggag cccgtgtcca gatgtaatgc
     3481 aaggacactg tagctgcatg tgcagaagag gtccccctgt gctgtgttat cctcatactg
     3541 aaacaaagca tccttcactc ccacagtcag atcatcttat tgtggaagga gccaggcact
     3601 cttgacatct gacgggcaga tcatcttatt tctgaccttc attcaggagt ttgttgcact
     3661 tttctagaaa ccctcccccc cccaaaaaaa aaaccagttt gtttgaccta tttatgaatt
     3721 tctattttct cccgtttaag aacccactag caaccaaaca tgtacttcgg gggtttctgt
     3781 gtgtccctgt gcttgaccgg tttctagatt gcccagtggg ccagtgagcg tgttcaccaa
     3841 gcacattatc atcttgccat gcaagcggtg agtgagaaaa tcacaggcag gtatccctgc
     3901 ccatgcatgg aagccgtcct tctagagtgt tgccatgctt ccgtaaatgc ttggggttac
     3961 tcctgcgttg ctttgcctta ggatcgtcca gttctgtagt atttatatgt aagatgcact
     4021 ctttccaaga gtttccattc catttggctg gaaacacagt agaacctatg caccagcatt
     4081 agtcacaagt cttgagacgt ggccggtaga tggcaggtag tgccacacca ggctcagcat
     4141 ttcctgccca ctttctgtgt cgcttcaggt gaaaagcctt agcagtaagt tgtaattaat
     4201 catttaagtc tacccaggac ggtgaccact ttcaaataac tgtgtcacgt gaaagataat
     4261 gcccatatcc aggtcagtat cggctatgcc tttttgttcc tggtcacaca attaaatgac
     4321 tagagaagtt tgagtaaaca tgcaaacaaa tagttctgtg gttaaggggt ttcttctctt
     4381 aatcttattc ttgaaggata aaatttgaat taaatctaaa atgcctgtca cctaagtgtc
     4441 gaaagaactt tggccttttt aagacttgtt ttgcaaaagc taagaagcca gactgttaca
     4501 atggatttgc ccacaaaaga gaggccagat acatgcatgg taaaagactt tgtttctagt
     4561 gtctcacctg taccatcaag tgctcttgat acataagtag tcaaatacat aatgtcattc
     4621 aggaaagtat taaaggaaat gacttgggag acacacagtc aggaagactc ctacaactgc
     4681 cttggttccc ccacctttct tcctgcgagt tcagctagga cacgattctt ctcgccagtg
     4741 cttagatgaa ctggaaaagc gattccttac aatgtgtgtg tgcactataa actacctcac
     4801 cagcctttct ggaggaaaag aatccagtgg actttccctt ttgtttgttc gtttgtttgt
     4861 ctgactctag tcactgccgt tagctttcag tgtgtgttgt gatcctacgt cattcatgct
     4921 gtaaatgagt tctagtgtaa gcatgggaac aaacatacca cgggaatgtg ccctgctgtg
     4981 tgctctgggg gagtctgtgt gtgtcccacg ggctgctcct gtttctaacc atgcggggga
     5041 cacgagttgc tgggaactgg ggttcatcct ttctggcagt gtttcccact gctctccatg
     5101 gatttcatct gactgaagac tgaggagaca aagatgtctt cctctctctc tctctctctc
     5161 tctctagcaa gtgcgtttta atgatttttc acgtgtatgt ctgacttgcc tggcaagact
     5221 gcgtatgagg ggcttgactc ctcctcttga caggcatttt caactatttt ccataaatat
     5281 gccacttaat aaaaagtaat atgacataag attaaaa
//
