LOCUS       NM_176837               3616 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Rho GTPase activating protein 18 (Arhgap18), mRNA.
ACCESSION   NM_176837
VERSION     NM_176837.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Coleman,P.R., Lay,A.J., Ting,K.K., Zhao,Y., Li,J., Jarrah,S.,
            Vadas,M.A. and Gamble,J.R.
  TITLE     YAP and the RhoC regulator ARHGAP18, are required to mediate
            flow-dependent endothelial cell alignment
  JOURNAL   Cell Commun Signal 18 (1), 18 (2020)
   PUBMED   32013974
  REMARK    GeneRIF: YAP and the RhoC regulator ARHGAP18, are required to
            mediate flow-dependent endothelial cell alignment.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3616)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3616)
  AUTHORS   Lay,A.J., Coleman,P.R., Formaz-Preston,A., Ting,K.K., Roediger,B.,
            Weninger,W., Schwartz,M.A., Vadas,M.A. and Gamble,J.R.
  TITLE     ARHGAP18: A Flow-Responsive Gene That Regulates Endothelial Cell
            Alignment and Protects Against Atherosclerosis
  JOURNAL   J Am Heart Assoc 8 (2), e010057 (2019)
   PUBMED   30630384
REFERENCE   5  (bases 1 to 3616)
  AUTHORS   Thompson,W.R., Yen,S.S., Uzer,G., Xie,Z., Sen,B., Styner,M.,
            Burridge,K. and Rubin,J.
  TITLE     LARG GEF and ARHGAP18 orchestrate RhoA activity to control
            mesenchymal stem cell lineage
  JOURNAL   Bone 107, 172-180 (2018)
   PUBMED   29208526
  REMARK    GeneRIF: mechanical activation of RhoA in mesenchymal progenitors
            is dependent on LARG, while ARHGAP18 limits RhoA delineated
            cytoskeletal structure in static cultures
REFERENCE   6  (bases 1 to 3616)
  AUTHORS   Liu,R., Lo,L., Lay,A.J., Zhao,Y., Ting,K.K., Robertson,E.N.,
            Sherrah,A.G., Jarrah,S., Li,H., Zhou,Z., Hambly,B.D.,
            Richmond,D.R., Jeremy,R.W., Bannon,P.G., Vadas,M.A. and Gamble,J.R.
  TITLE     ARHGAP18 Protects Against Thoracic Aortic Aneurysm Formation by
            Mitigating the Synthetic and Proinflammatory Smooth Muscle Cell
            Phenotype
  JOURNAL   Circ Res 121 (5), 512-524 (2017)
   PUBMED   28701309
  REMARK    GeneRIF: ARHGAP 18 was identified as a novel protective gene
            against thoracic aortic aneurysm formation.
REFERENCE   7  (bases 1 to 3616)
  AUTHORS   Lovelace,M.D., Powter,E.E., Coleman,P.R., Zhao,Y., Parker,A.,
            Chang,G.H., Lay,A.J., Hunter,J., McGrath,A.P., Jormakka,M.,
            Bertolino,P., McCaughan,G., Kavallaris,M., Vadas,M.A. and
            Gamble,J.R.
  TITLE     The RhoGAP protein ARHGAP18/SENEX localizes to microtubules and
            regulates their stability in endothelial cells
  JOURNAL   Mol Biol Cell 28 (8), 1066-1078 (2017)
   PUBMED   28251925
  REMARK    GeneRIF: In endothelial cells, ARHGAP18 may act as a significant
            regulator of vascular homeostasis.
REFERENCE   8  (bases 1 to 3616)
  AUTHORS   Yeung,C.Y., Taylor,S.H., Garva,R., Holmes,D.F., Zeef,L.A.,
            Soininen,R., Boot-Handford,R.P. and Kadler,K.E.
  TITLE     Arhgap28 is a RhoGAP that inactivates RhoA and downregulates stress
            fibers
  JOURNAL   PLoS One 9 (9), e107036 (2014)
   PUBMED   25211221
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3616)
  AUTHORS   Chang,G.H., Lay,A.J., Ting,K.K., Zhao,Y., Coleman,P.R.,
            Powter,E.E., Formaz-Preston,A., Jolly,C.J., Bower,N.I., Hogan,B.M.,
            Rinkwitz,S., Becker,T.S., Vadas,M.A. and Gamble,J.R.
  TITLE     ARHGAP18: an endogenous inhibitor of angiogenesis, limiting tip
            formation and stabilizing junctions
  JOURNAL   Small GTPases 5 (3), 1-15 (2014)
   PUBMED   25425145
  REMARK    GeneRIF: Results describe ARHGAP18 as a novel negative regulator of
            sprouting by acting dualistically to limit tip cell formation and
            to maintain junctional integrity.
REFERENCE   10 (bases 1 to 3616)
  AUTHORS   Shalom-Barak,T., Zhang,X., Chu,T., Timothy Schaiff,W., Reddy,J.K.,
            Xu,J., Sadovsky,Y. and Barak,Y.
  TITLE     Placental PPARgamma regulates spatiotemporally diverse genes and a
            unique metabolic network
  JOURNAL   Dev Biol 372 (1), 143-155 (2012)
   PUBMED   22967998
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC158631.6 and
            AC101709.11.
            
            On Nov 17, 2006 this sequence version replaced NM_176837.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030858.1, SRR11927934.8530875.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               AC158631.6         118584-118768
            186-382             AC158631.6         192004-192200
            383-618             AC158631.6         195947-196182
            619-682             AC158631.6         200946-201009
            683-858             AC101709.11        141133-141308       c
            859-1024            AC101709.11        131779-131944       c
            1025-1116           AC101709.11        129101-129192       c
            1117-1194           AC101709.11        124631-124708       c
            1195-1354           AC101709.11        122815-122974       c
            1355-1437           AC101709.11        121095-121177       c
            1438-1644           AC101709.11        113878-114084       c
            1645-1785           AC101709.11        111993-112133       c
            1786-1910           AC101709.11        89134-89258         c
            1911-1972           AC101709.11        85411-85472         c
            1973-3616           AC101709.11        83217-84860         c
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 14.12 cM"
     gene            1..3616
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /note="Rho GTPase activating protein 18"
                     /db_xref="GeneID:73910"
                     /db_xref="MGI:MGI:1921160"
     exon            1..185
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2064
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /note="rho-type GTPase-activating protein 18"
                     /codon_start=1
                     /product="rho GTPase-activating protein 18"
                     /protein_id="NP_789807.1"
                     /db_xref="CCDS:CCDS23757.1"
                     /db_xref="GeneID:73910"
                     /db_xref="MGI:MGI:1921160"
                     /translation="MNWLSSSSGVVLTAYHPSGKDQVAGDSHVKGGDEATSSRRYGQY
                     TINQEGSTKVPERPPFDRSSSQDSLDESMEAYWTELENIKRSNENRQEGQEAIVVKEP
                     DEGELEEEWLKEAGLSNLFGESIDDPQESILFLSTLTRTQAAAVQKRVETVSQTLRKK
                     NKQHHIRDVRDIFAQQREAQEKPPDDSDLRSVRTNENKGQGKDDQPSSGAVDSKEQIS
                     RVPEDTPASETDINLEVSFAEQAVNQKEFSKERTQKISSNDSLPSFRLPKDKTGTTRI
                     GDLAPQDMKKVCSLSLIELTALYDVLGLEFKQQKAVKIKTRDSGLFGIPLTILLEQDQ
                     RKVPGTRIPLIFQKLISRIEEGSLETEGLLRIPGAAMRIKNLCQELEAKFYEGTFNWE
                     SVKQHDAASLLKLFLRELPQPLLSMEYLKAFQAVQNLPTRKEQLQALNLLVILLPDAN
                     RDTLKALLEFLQRVIDNKEKNKMTAGNVAMVMAPNLFMCHTLGLKSSEQREFEMAAGT
                     ANVMHLLIRYQKILWTIPKFIVIQVRKQNIENQKKERKAMKKLLKKMAYDREKHEKQD
                     KTANGADVPQGVIRVQAPHLSKVSMAIQLTEELKASDVLARFLSQESGVAQTLKKGEV
                     FLYEIGGNIGERCLDDDTHMKDLYQLNPNAEWVIKSKPV"
     exon            186..382
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            383..618
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            619..682
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..858
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1024
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1116
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1194
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1354
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1437
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1644
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1785
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1910
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..1972
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..3616
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3592..3597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3616
                     /gene="Arhgap18"
                     /gene_synonym="4833419J07Rik"
                     /note="major polyA site"
ORIGIN      
        1 gttggtcagg cgagacagga acttcattcc cctctgtgtc agaatcccag aaattatgcc
       61 ccttctctca ccatgaactg gctatcgagt tcctcgggag ttgtgctaac tgcttaccac
      121 cccagcggca aggaccaggt cgctggggac agccatgtaa agggaggcga cgaagccacc
      181 tccagtcgca gatatggcca atacaccata aaccaagaag gctccaccaa agttcccgag
      241 aggcctccat ttgatcgatc gagttctcag gattccttgg atgaatcaat ggaagcctat
      301 tggacagaac tagaaaacat caagaggtcg aatgaaaatc gccaagaagg tcaagaggcc
      361 atagttgtca aagaacctga tgagggagaa ctggaagaag aatggctcaa agaagcaggt
      421 ctatccaatc tttttggaga gtctattgat gacccgcagg aaagcattct gtttttatct
      481 acactgaccc ggactcaggc agcagctgtt cagaaacggg tggaaacggt ctcccagacc
      541 ttgaggaaga aaaacaaaca gcaccacatt cgtgacgtca gagacatatt tgctcaacag
      601 agagaggcac aagaaaaacc tccagatgac tcagacttgc ggtcagtcag aacaaacgaa
      661 aacaaaggcc aaggaaaaga tgatcagcca tctagtgggg ctgtcgatag caaggagcag
      721 atctcacggg tgcctgagga cacacctgcc tctgaaacag acatcaatct ggaggtgtca
      781 tttgcggagc aagctgtcaa tcagaaagag ttcagtaagg agaggacgca gaagatcagc
      841 agcaacgact ctttgcccag tttcagactg ccaaaagata aaacaggtac gacaaggatt
      901 ggagacttgg caccccaaga catgaagaaa gtttgctctc tatccctgat tgaactcact
      961 gccctgtatg atgtgttggg cttggaattc aaacaacaga aagctgtgaa aatcaaaacc
     1021 agagactctg gtctttttgg tattccttta acgatattgt tagaacaaga tcaaagaaaa
     1081 gtgcctggaa cacgaatacc cttgatcttt caaaaactga tttctcgaat tgaagaaggc
     1141 agtctggaaa ccgagggcct cctacggata ccaggagcag ccatgagaat caagaatctt
     1201 tgccaagaac tagaagcgaa attttacgag gggactttca attgggaaag tgtcaaacag
     1261 cacgatgcgg ccagcctgct gaagctcttt ctgagagaat tgccccagcc actgctgagc
     1321 atggagtatc tcaaagcctt ccaagctgtc cagaatcttc caaccagaaa ggaacagttg
     1381 caggctttga acctccttgt cattcttcta cctgacgcaa acagagacac actgaaggcc
     1441 ttgcttgaat ttctccaaag agtcatagat aacaaagaga agaacaagat gacagctggg
     1501 aatgtagcaa tggtcatggc cccaaatctc ttcatgtgcc acacgctggg cttgaagtcc
     1561 agtgaacagc gagaatttga aatggcagct gggacagcaa atgtcatgca cttattgatt
     1621 aggtaccaga aaattctatg gacaatccct aaatttattg taatccaagt gaggaaacag
     1681 aatattgaga atcaaaagaa ggaaaggaaa gccatgaaga aattgctgaa gaaaatggct
     1741 tatgaccggg aaaaacatga aaagcaagat aaaactgcaa atggtgctga tgttcctcag
     1801 ggagtgattc gagtgcaagc tccccatctt tcaaaagttt ccatggcaat acagctaact
     1861 gaagaactaa aagccagcga tgtgcttgcc aggtttctca gccaagaaag tggggttgcc
     1921 cagacactca agaaaggaga agttttcctg tatgagattg gaggaaatat tggggaacgt
     1981 tgcctcgatg acgacacaca catgaaggac ttgtaccaac tgaacccgaa tgccgagtgg
     2041 gtcataaaat ccaagccagt gtagacgaca accccgagga cgtgtcatca ttcttgctgg
     2101 gtcgagagtg taaataaaag tagcaggttt cttaactatt cagctgcacc caattattac
     2161 aaattgatac cctcaagcct taaaattata gatttttttt tgtggcttgc acaattgttg
     2221 ttattattgt tagaaaataa atattgctgg agtaagaaga aaggggctta attagcttgt
     2281 gggctgttta tacacaattt aaaatgtgtt catatgtgac agctttctat tgtatgagat
     2341 aacttcttta ccacttttcc cccccttttg gtactaagta ttgaacaaga actttgctca
     2401 tagtgggcaa atgctttaag acacctagta atatccccag cagattaaat actctttact
     2461 aataatagat ttactaatag agttttgggc tagttacatt ttaagaatat tgtctcccct
     2521 tccttacccc tccctattta tcttccctta ataaaatttg ctttttaact agaaagagat
     2581 agttaatagc aggtatagaa agccatgaag gaaaagataa ctcagcagta ttcttgttac
     2641 cacaaaatgt tagtactcat ggctttgaat catctgtcta aacaacgcag gcagttgttt
     2701 ttttttgagc ttattggact tatgtgcaca aagctgtacc catcgtcaag acccagactg
     2761 ccaggacctt ctaattatca ccaaagcatt aaatgtttaa acactcagga gaaacaatat
     2821 ggagccacac ggactaactg agtcatctat gaccacaatg ggaaaagcta gatcacttta
     2881 tgtgtacaac actctttcag atagaatctg aaaaatcaca tatcaagtta cttggcaaca
     2941 ttatacacag cataatagta caacctggaa caattggaga atacattgta aaatgccact
     3001 ttccctcctt tcagagtaag tcttcagggt atgacaatca tttatgtgaa caagcttttg
     3061 tcggtgtgtg tattttaaag aatctgagtc agtttgttaa gatgctgtgc aaagtattat
     3121 ccccagggct ttttgtgcac agaccacact gtgacagaac acagacctct gctgtaaata
     3181 agagaaatga aataagagca atgttcacta tttcataagg gtagagtgtc actgacgaaa
     3241 gatggcatgt gtggatgagc cactggcata tgatgacact gttcatagtt ctttagggga
     3301 agccaaacac acgtcctcag aagctcttga tgccgctcta ggaaagaaag tgctgttcat
     3361 gagaacaggg aagtgttgag catacaatga cgagagcagc ctgtggatgg gtgctttggt
     3421 agcactagga atgggtgctg tggttatgcc atggggattc attcgtgcca ttgctttttg
     3481 tatttaaaga aatgttataa ttaaatggat ttttctaaac aatcttctct tggaattatt
     3541 acattacagg ggtatgttca cgagtctatg tttgtccttt taaattattt taataaacag
     3601 tctattttca atgtaa
//
