LOCUS       NM_001033258            8216 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus ARFGEF family member 3 (Arfgef3), mRNA.
ACCESSION   NM_001033258 XM_125524 XM_897216 XM_907736 XM_920540
VERSION     NM_001033258.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Iskusnykh,I.Y., Fattakhov,N., Li,Y., Bihannic,L., Kirchner,M.K.,
            Steshina,E.Y., Northcott,P.A. and Chizhikov,V.V.
  TITLE     Lmx1a is a master regulator of the cortical hem
  JOURNAL   Elife 12, e84095 (2023)
   PUBMED   37725078
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8216)
  AUTHORS   Liu,T., Li,H., Hong,W. and Han,W.
  TITLE     Brefeldin A-inhibited guanine nucleotide exchange protein 3 is
            localized in lysosomes and regulates GABA signaling in hippocampal
            neurons
  JOURNAL   J Neurochem 139 (5), 748-756 (2016)
   PUBMED   27696409
REFERENCE   4  (bases 1 to 8216)
  AUTHORS   Li,H., Liu,T., Lim,J., Gounko,N.V., Hong,W. and Han,W.
  TITLE     Increased biogenesis of glucagon-containing secretory granules and
            glucagon secretion in BIG3-knockout mice
  JOURNAL   Mol Metab 4 (3), 246-252 (2015)
   PUBMED   25737957
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8216)
  AUTHORS   Liu,T., Li,H., Gounko,N.V., Zhou,Z., Xu,A., Hong,W. and Han,W.
  TITLE     Detection of insulin granule exocytosis by an electrophysiology
            method with high temporal resolution reveals enlarged insulin
            granule pool in BIG3-knockout mice
  JOURNAL   Am J Physiol Endocrinol Metab 307 (7), E611-E618 (2014)
   PUBMED   25139048
REFERENCE   6  (bases 1 to 8216)
  AUTHORS   Li,H., Wei,S., Cheng,K., Gounko,N.V., Ericksen,R.E., Xu,A., Hong,W.
            and Han,W.
  TITLE     BIG3 inhibits insulin granule biogenesis and insulin secretion
  JOURNAL   EMBO Rep 15 (6), 714-722 (2014)
   PUBMED   24711543
REFERENCE   7  (bases 1 to 8216)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 8216)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC153558.7 and
            AC153561.5.
            
            On Oct 19, 2007 this sequence version replaced NM_001033258.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147679.1, SRR17784643.789771.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-218               AC153558.7         172281-172498       c
            219-270             AC153558.7         162798-162849       c
            271-352             AC153558.7         128069-128150       c
            353-484             AC153558.7         125293-125424       c
            485-553             AC153558.7         106565-106633       c
            554-676             AC153558.7         93631-93753         c
            677-719             AC153558.7         90086-90128         c
            720-798             AC153558.7         89337-89415         c
            799-903             AC153558.7         82950-83054         c
            904-1237            AC153558.7         81274-81607         c
            1238-1350           AC153561.5         225191-225303       c
            1351-2249           AC153561.5         223548-224446       c
            2250-2416           AC153561.5         213067-213233       c
            2417-2582           AC153561.5         212150-212315       c
            2583-2690           AC153561.5         208206-208313       c
            2691-2906           AC153561.5         207667-207882       c
            2907-3017           AC153561.5         207418-207528       c
            3018-3168           AC153561.5         205008-205158       c
            3169-3486           AC153561.5         202535-202852       c
            3487-3620           AC153561.5         198513-198646       c
            3621-3766           AC153561.5         197035-197180       c
            3767-3946           AC153561.5         194236-194415       c
            3947-4091           AC153561.5         190525-190669       c
            4092-4214           AC153561.5         188591-188713       c
            4215-4318           AC153561.5         185708-185811       c
            4319-4463           AC153561.5         185032-185176       c
            4464-4592           AC153561.5         180794-180922       c
            4593-4769           AC153561.5         177821-177997       c
            4770-4884           AC153561.5         176573-176687       c
            4885-4987           AC153561.5         175083-175185       c
            4988-5119           AC153561.5         174715-174846       c
            5120-5241           AC153561.5         172295-172416       c
            5242-6454           AC153561.5         168517-169729       c
            6455-8216           AC153561.5         165398-167159       c
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 7.79 cM"
     gene            1..8216
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="ARFGEF family member 3"
                     /db_xref="GeneID:215821"
                     /db_xref="MGI:MGI:106387"
     exon            1..218
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..6646
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 3"
                     /protein_id="NP_001028430.1"
                     /db_xref="CCDS:CCDS23712.1"
                     /db_xref="GeneID:215821"
                     /db_xref="MGI:MGI:106387"
                     /translation="MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTVVKIPPHL
                     LREKCLLPLQLALESKNVKLAQHALAGMQKLLSEERFVSMETDSDEKQLLNQILNAVK
                     VTPSLNEDLQVEVMKVLLCITYTPTFDMNGSAVLKIAEVCIETYTCSCHQRSINTAVR
                     ATLSQMLGDLTLQLRQRQENTIIENPDAPQEFRSQGLTVEALCDDVISVLAVLCEKLQ
                     ASINDSQQLQLLYLECILSVLSSSSSSMHLHRGFTDLIWKSLCPALVVILGNPIHDKT
                     ITSAHSTSTSTSMESDSASLGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVG
                     SVDSMKPVLQSLYHRVLLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSIESEPRKR
                     SISKRKSHLDLLKLIMDGMTEACIKGGIEACYAAVSCVCTLLGALDELSQGKGLNDTQ
                     VQQLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEHTPWESGNERSPDISISVT
                     TDTGQTTLEGELGQTTPEDHKNGLKSPAIQEGKGTMGKVSEPEAIDQPDVVQRSHTVP
                     YPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVS
                     DIGSDNCSLADEEQTPRDYIGHRSLRTAALSLKLLKNQEADQHSARLFIQSLEGLLPR
                     LLALSSVEEVDSALQNFASTFCSGMMHSPGFDGGSSLSFQMLMNADSLYTAAHCALLL
                     NLKLSHGDYYRKRPTVAPGMMKEFMKQVQTSGVLMVFSQAWLEELYHQVLDRNMLGEA
                     GYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASASVESPFTQSRRLDDSTVAGVAF
                     ARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMSLDGL
                     RKAARLSCALGVAANCASALAQMAAASCVQEEKEERQSQEPSDALAQVKLKVEQKLEQ
                     MGKVQGVWLHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGIS
                     QPPLTIHQPQKTSGSSGLLGEIEFKSSSQEQSLEQGPSLNTAPVVQPHSIQELVRECS
                     RGRTSDFRGGSLSGNSAAKVVLSLSTQADRLFDDATDKLNLTALGGFLYQLKKASQSQ
                     LFHSVTDTVDYSLTMPGEVKSTQDQKSALHLFRLGDAMLRIVRSKARPLLHVMRCWSL
                     VAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQLE
                     LCDEDVQDQVVTSIGELVEVCSAQIQSGWRPLFSALETVRSGNKSEVKEYLVGDYSMG
                     KGQAPVFDVFEAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGAGAT
                     STDLCLPALDYLRRCSQLLAKIYKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVL
                     SDFDDDTGLIEVWIILLEQLTAAVSNCPRQHQPPTLDLLFELLRDVTKTPGPGFGIYA
                     VVHLLLPVMSLWLLRSHKDHSYWDVASANFKHAIGLSCELVVEHIQSFLHSDIRYESM
                     INTMLKDLFELLVVCVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAF
                     SATLKPVKDLLGCFHGGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDT
                     QCSPKTPNNFDHAQSCQLIIELPHDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDI
                     LLEEFVKGPSPGEEKTVQVPDTKLAGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPG
                     LKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAVLTNQETITAEQVKKVLFEEEER
                     SSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNADWVWLVKRLH
                     KLCMELCNHYIQMHLDLESSLEEPLTFKSDPFFILPSFQSESSTPSTGGFSGKNTPSE
                     DDRREHLSEPQSLRVGSGDMLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTIS
                     KLMTEYKKRRQPHNLPPFPKEVKVDKKGEPLGPRGPDSPLLQRPQHLIDQGQMRHSFS
                     AGPELLRQEKRPRSGSTGSSLSVSVRDAEAQIQAWTNMVLTVLNQIQILPDQTFTALQ
                     PAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIIT"
     exon            219..270
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            271..352
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            353..484
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            485..553
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            554..676
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            677..719
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            720..798
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            799..903
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1237
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1350
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..2249
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2416
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2582
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2690
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2906
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            2907..3017
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3168
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3486
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3620
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..3766
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3767..3946
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..4091
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4092..4214
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4215..4318
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4319..4463
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4464..4592
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4593..4769
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4770..4884
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4885..4987
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            4988..5119
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            5120..5241
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            5242..6454
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     exon            6455..8216
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7687..7692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="hexamer: AATATA"
     polyA_site      7706
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="major polyA site"
     regulatory      7780..7785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="hexamer: TATAAA"
     regulatory      7798..7803
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="hexamer: AGTAAA"
     polyA_site      7803
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
                     /note="major polyA site"
     polyA_site      7818
                     /gene="Arfgef3"
                     /gene_synonym="9630004D11; B930094H20Rik; BIG3;
                     D10Bwg1379e; mKIAA1244"
ORIGIN      
        1 gacgtggcct gtggcgcgga gcgggtgtga ttcatcagcg gccgccgaga gccatgggga
       61 cgagtgccga ggcctcctag gcgcgtcccc tgcgcgatct cggggtcggt ggcctggctc
      121 taggaaggcc aagatggaag agatcctgag gaagctgcag agagatgcgt ccgggagcaa
      181 atacaaagcc atcaaagaga gctgcacctg ggccctggaa accctaggtg gcctggacac
      241 tgttgtgaag atccctcccc acttgttgag ggagaaatgc ttgctgcctc tccagttggc
      301 tttggaatca aaaaatgtga agctggccca acatgctttg gcaggaatgc agaagctcct
      361 gtcagaagag agatttgtct ccatggagac agactccgat gagaagcagc tgctcaatca
      421 gatcctgaat gcagtgaaag tgacaccgtc actcaatgag gacctgcagg tggaagtgat
      481 gaaggtttta ctgtgcatca cctacacccc gacatttgac atgaatggga gtgccgttct
      541 gaagatcgca gaggtgtgca tcgagaccta cacgtgcagc tgccaccagc gcagtatcaa
      601 cactgccgtg cgtgcaacgc tcagtcagat gctgggtgac ctgactttac agttacggca
      661 gagacaggag aatacgataa ttgaaaaccc agatgcccca caggaattca ggagtcaagg
      721 cttaacagta gaggcccttt gtgatgacgt catctctgtg ctcgcagttc tgtgtgagaa
      781 gctccaagcc tccataaatg acagccagca gctacagctt ctgtacctgg aatgcatcct
      841 ctccgtgctc agcagctcct cttcctccat gcacctgcac aggggcttca cagacctcat
      901 ctggaaaagc ctctgccctg ctctcgtggt gatcctgggg aaccctattc acgacaaaac
      961 aatcacctct gctcacagca ccagcaccag caccagcatg gagtcggact ctgcctctct
     1021 tggtgtgtct gaccacggcc ggggctcagg ctgctcctgc actgcaccca ccctcagtgg
     1081 gcctgtggcc cgcaccatct attaccttgc agctgagctt gtccgcttag tggggtcagt
     1141 ggactccatg aagccagtgc tccagtccct ctatcaccga gtgctcctgt atcccccacc
     1201 ccagcaccga gtggaagcca tcaagatcat gaaagagata cttgggagcc cacagcgtct
     1261 gtatgacttg gcaggaccaa gctctattga gtctgaacca aggaaaagat ctatttccaa
     1321 aagaaagtct cacttggatc ttctcaaact catcatggac ggcatgacag aagcatgcat
     1381 caaaggaggc attgaagcct gctacgctgc tgtgtcctgt gtctgcacct tgcttggggc
     1441 tctggatgaa ctcagccaag ggaaaggctt gaatgacact caagtgcagc agctgcttct
     1501 gcgccttgaa gagctgaggg atggggccga gtcgagccgg gactccatgg aaattaatga
     1561 ggctgacttc cgctggcagc ggagagtgct gtcttcggag cacactccat gggagtcagg
     1621 caatgagagg agccctgaca tcagcatcag tgtcaccaca gacacaggcc agactacctt
     1681 ggaaggagaa ttgggtcaga cgacaccaga ggaccacaag aatgggctca agtcaccagc
     1741 cattcaggaa ggcaagggga ccatgggcaa agtgtcagaa cctgaggcta tagaccagcc
     1801 agatgtggtt cagagaagcc acacagtccc ttaccccgat atcaccaact tcctgtcagt
     1861 ggactgcagg acaaggtcct acggatctag gtatagtgag agcaacttca gtgtagatga
     1921 ccaagacctg tcgaggactg aatttgattc ctgtgatcag tattccatgg cggcagaaaa
     1981 ggactcaggg aggtctgatg tgtcagacat tgggtcagac aactgttccc tggctgatga
     2041 agaacagacc cctcgggact acataggcca tcggtccctt cgaaccgctg ctttgtctct
     2101 caaactgcta aagaaccagg aggcagacca gcacagcgcc cgactgttta tacagtccct
     2161 tgagggcctc ctaccccggc tgctggccct gtccagtgtg gaagaggtgg actccgccct
     2221 tcagaacttt gcctctactt tctgctcagg catgatgcac tctcctggct ttgatggagg
     2281 cagtagcctg agcttccaga tgctgatgaa tgcagacagc ttgtacacag cggcacactg
     2341 tgccctactg ctcaacctga agctctccca cggtgactac taccggaagc gaccaaccgt
     2401 ggcaccaggc atgatgaaag agttcatgaa acaggtgcag accagcggcg tgctcatggt
     2461 cttctcccag gcctggctcg aggaactgta ccatcaggtg ctggacagaa acatgctggg
     2521 agaagctggc tactggggca gccctgagga caacagcctt cccctcatca ccatgctcac
     2581 cgatatcgat ggtctagaga gtagcgcaat cggtggccag ttgatggcgt cagcttcagt
     2641 ggagtcacct ttcacccaga gcaggagact tgatgactcc acggtagcag gtgttgcatt
     2701 tgcccgttat attttggttg gctgctggaa gaacctgatt gatactctgt caaccccctt
     2761 gactggtcgg atggcaggaa gctccaaggg gttggccttc atccttggag ctgagggtat
     2821 caaagagcag aaccagaaag agcgagatgc catctgcatg agccttgatg ggctaaggaa
     2881 agctgcgcga ctgagctgtg cactcggggt cgctgctaac tgtgcctcag ctcttgccca
     2941 aatggcagct gcatcctgtg tccaagaaga gaaagaagaa aggcaatccc aagagcccag
     3001 tgatgcccta gcacaagtga aactaaaagt ggagcagaaa ctggagcaga tggggaaggt
     3061 gcagggggtg tggctgcaca cagcccacgt cttgtgcatg gatgccattc tcagcgtagg
     3121 cctggagatg ggaagccaca acccggactg ctggccacac gtgttcaggg tgtgtgagta
     3181 tgtgggtaca ctggagcaca cccacttcag cgatggtatc tcccagcccc ctctgaccat
     3241 ccatcagcca cagaagacat ctgggagctc tggcctcctt ggggaaattg agtttaagag
     3301 ctcatcccag gagcagagcc tggagcaggg gccttccctg aacacagccc ctgtggtcca
     3361 gccacactcc atccaggagc ttgttcggga atgtagccgt ggccggacct cggacttccg
     3421 gggaggtagt ctgagtggga acagtgctgc caaggtggtg ctcagccttt ccacccaggc
     3481 cgacaggctc tttgatgacg cgaccgacaa gttgaactta acagccttgg gagggttcct
     3541 ctaccaactc aaaaaagcat ctcagtccca gcttttccat tcggtgacag acacagtcga
     3601 ttactctctg acaatgccag gtgaagtcaa gtctacccag gatcaaaaaa gcgctctcca
     3661 cctgttccgc ctgggggatg ccatgctgag gattgtgagg agcaaagccc ggcccctgct
     3721 ccacgtgatg cgctgctgga gcctcgtggc cccgcacctg gtggaggctg cctgccataa
     3781 agaacgccat gtgtctcaga aggccgtttc cttcatccat gatatcttga cagaggttct
     3841 cactgactgg agtgagccac ctcacttcca ctttaacgaa gcactcttcc ggccctttga
     3901 gcgcatcatg cagctagagc tctgtgatga agatgtccaa gaccaggtgg tcacctccat
     3961 tggtgagctg gttgaggtgt gctctgcaca gatccagtcg ggatggcgac ccctgttcag
     4021 tgctctggag accgtgcgca gtgggaacaa gtcagaggtg aaggagtacc tggttgggga
     4081 ctactctatg ggaaaaggcc aggctcctgt gttcgatgtg tttgaagctt ttctcaatac
     4141 cgacaacatc caggtctttg ctaacgcagc tactagttac atcatgtgcc ttatgaaatt
     4201 tgtcaagggg ctgggggagg tagactgtaa agagattgga gactgtgttc ctggagctgg
     4261 agctacatct acagacctgt gcctgcctgc cttggattac ctcagacgtt gttctcagtt
     4321 attggccaag atctacaaaa tgcccttaaa gccaatattc cttagtgggc gacttgccag
     4381 tttgccacga aggcttcagg agcagtcggc aagcagtgaa gatggaattg aatcagtcct
     4441 gtcagatttt gatgatgaca ctggtctgat agaagtctgg atcatcctgt tggagcagct
     4501 aacagcagct gtgtccaact gtccaaggca acaccaacca cccaccttag atttactctt
     4561 tgagctgttg agagatgtta ccaaaacacc tggccctggg tttggaatct atgcagttgt
     4621 tcaccttctc cttcctgtga tgtctctgtg gctcctccgt agccacaagg accattccta
     4681 ctgggatgtg gcatctgcta acttcaagca cgccattggt ctgtcctgcg agctggtggt
     4741 ggaacacatt caaagctttc tacactcaga catcaggtat gagagtatga tcaacaccat
     4801 gctgaaggat ctctttgagc tactggtagt atgcgtggcc aagcccacag aaaccatctc
     4861 cagggtgggc tgttcctgca ttaggtacgt cctcgtgaca gcaggtcccg tgttcacgga
     4921 ggagatgtgg agacttgcat gctgtgccct tcaggacgcc ttctcagcca ccctcaagcc
     4981 agtgaaggac ctgctaggct gcttccacgg tggtacagag ggcttcagtg gggaaggctg
     5041 ccaggtgcgc gtagcagcac cctcttcctc cccgagtgct gaggctgagt actggcgcat
     5101 ccgggccatg gcccagcagg tgtttatgtt ggacacccag tgctcaccaa agactccaaa
     5161 caacttcgat cacgctcagt cctgtcagct catcattgaa ctgcctcatg atgagaaacc
     5221 aaatggacat gccaagaaaa gtgtctcttt cagggagatt gtggttagct tgctctcgca
     5281 ccaagttctg ctccagaacc tgtatgacat tctgctggaa gagtttgtca aaggcccctc
     5341 tccgggagaa gagaagacag tccaagtacc agacaccaag ctggccggct tcctcagata
     5401 catttccatg cagaacctgg ctgtgatatt tgacctgctg ctggactcct ataggactgc
     5461 ccgggaattc gacaccagcc cagggctgaa atgcctgctg aagaaagtgt ctggcattgg
     5521 aggagcagcc aacctctacc gccagtcggc catgagcttt aacatttact ttcacgccct
     5581 ggtttgcgcg gttctcacca accaagaaac tatcacagcc gagcaagtaa agaaggtgct
     5641 attcgaagag gaggagagga gctcggattc ctcccaacag tgctcgtcgg aggatgaaga
     5701 catctttgag gagactgcgc aggtgagccc cccacgaggc aaggagaaaa ggcaatggcg
     5761 ggcacgcctg ccgtcgctca gtgtgcagcc agtaagcaat gcagactggg tgtggctggt
     5821 caagaggctg cacaagctgt gcatggagct ctgtaaccac tacatccaga tgcacctgga
     5881 cctggagagc agcttggagg agccacttac cttcaagagc gacccgttct tcattctccc
     5941 ctccttccag tccgaatcgt ccaccccatc aacaggtggc ttctccggga aaaacacccc
     6001 ctcggaagat gaccgcaggg agcacctgag tgagcctcag agtctgaggg tcggcagtgg
     6061 ggacatgctg atgctgcctc ccagccccaa gacagagaag aaggatcctg gccgcaagaa
     6121 agagtggtgg gagagtgcag ggaacaagat ttgcacaatg gccgctgaca agaccatctc
     6181 aaagctgatg actgagtaca agaagagaag gcagccacat aacctgcccc ccttccccaa
     6241 ggaagtcaaa gtagataaga aaggggagcc cttggggccc cggggtcctg actccccact
     6301 gcttcagcgg ccacagcatc tcatagacca agggcagatg cgccattcct tcagcgcagg
     6361 cccagagctg ctgaggcagg agaagagacc tcggtcaggc tccacaggaa gctcccttag
     6421 tgtctctgta cgggatgcag aagcgcagat ccaggcgtgg accaacatgg tgctaactgt
     6481 tctcaatcag attcagatcc ttccagacca gaccttcact gctctccagc cagctgtgtt
     6541 cccatgcatc agccagctga cctgtcacgt gactgacatc cgagtccgtc aagctgtgag
     6601 ggaatggctg ggaagagtgg gccgtgtcta tgacatcatc acttagagga ctcaaatcct
     6661 aaggaaatgc ctacagcatc tgcctgtcca tccagctgac tgcattttcc caaccaccat
     6721 cccactcaaa ccaggtctct ggaacctagt aacaaagctt tttctaagct ctccttacct
     6781 agtcaacctt gggttagtct ggatgccaac atagtataca agtggcagta gtgatctctg
     6841 gccttgaaca ttattacaga acagcccaca ttacttgata catttctaaa tttaatttcc
     6901 aagcgcattt gcttgcagtg ttattcccaa atgggtcatt ttcaacactt tcccttggaa
     6961 gcccttggct gatccatttt gagtcctcat tttcacaact aagtcctaag aaaacatttg
     7021 ccggtaagtc aagctgatat aaagtcatat atctggcacc agacataatc agaaaaactt
     7081 ataatctgat tatagtttgg ttgggaagaa agctgcagac gtgttttttt ttttttttct
     7141 cagtgttcca atcaagaaag aaattacttc taaaatatat tcaggaacat ttcaacgtta
     7201 ttcttaatgt ttttaggttt ttatgttgat attcataaat tccccaggta aggtactgtt
     7261 gtgcctttat gtctctgttt ttaaaggaag gaaattcttt ccaagcatga ttttaagtct
     7321 gtgacgttaa catgaagcac ccggtggtgc atgttgtcat gagacttgat actgactacc
     7381 ctgtgtgtac catgagtctt tgcttctgtt cccaaggcag acaaagaaaa gaaagaaaat
     7441 tggtgcctta gtctttgttg cacagatatt tctatgcccc acaattatct caacaataat
     7501 gtacgacact cagttttaag aaagatttag attcatttta tatcagtatc ttttggagga
     7561 aaggaactca gagtcctcca tttggtgagc atacttctca ggggaggggc aagaactgtg
     7621 ggatgggaag acagctggaa ggggtgggct ggggctgtga tagaatcatt gtaagtcttc
     7681 aactacaata tacagttgtg aaatgatggt gtcggttata cacacattag actgaatggg
     7741 aagggacaca gtgaatttag aaaactattt ttttttctat ataaaacact tctctctagt
     7801 aaatttactc tctttgaact catgttattc agaccaatat tttacctgga aatttctttt
     7861 tctaagtatt attagaaaat ggctttgaaa tatagaacca ctgaggtccc ccagagccct
     7921 taccacaata tattttatat tttttacatt ataataatga ctaaagcttt tcagacaagc
     7981 ccacttacac tctatagtat aggattagtt actgaagcat tcatggcatt taaccagtgc
     8041 ctaaaaatgt atttgtgcat catcctctaa aaatgaaacc tccactcttc agtattgaag
     8101 gatttaagag ggattgaatt ttatccagtg gtaatattca taaacgttac cctccatgaa
     8161 aactacatag atatccacag gatcagagca aggctctttg gcgtatgtgg gaagtc
//
