LOCUS       NM_001085495            8777 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus ARF guanine nucleotide exchange factor 2 (Arfgef2),
            mRNA.
ACCESSION   NM_001085495 XM_130646 XM_911012
VERSION     NM_001085495.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Wang,Y.Z., Fan,H., Ji,Y., Reynolds,K., Gu,R., Gan,Q., Yamagami,T.,
            Zhao,T., Hamad,S., Bizen,N., Takebayashi,H., Chen,Y., Wu,S.,
            Pleasure,D., Lam,K. and Zhou,C.J.
  TITLE     Olig2 regulates terminal differentiation and maturation of
            peripheral olfactory sensory neurons
  JOURNAL   Cell Mol Life Sci 77 (18), 3597-3609 (2020)
   PUBMED   31758234
  REMARK    Erratum:[Cell Mol Life Sci. 2021 Jul;78(14):5665-5666. doi:
            10.1007/s00018-021-03870-2.. PMID: 34156491]
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Farini,D., Cesari,E., Weatheritt,R.J., La Sala,G., Naro,C.,
            Pagliarini,V., Bonvissuto,D., Medici,V., Guerra,M., Di Pietro,C.,
            Rizzo,F.R., Musella,A., Carola,V., Centonze,D., Blencowe,B.J.,
            Marazziti,D. and Sette,C.
  TITLE     A Dynamic Splicing Program Ensures Proper Synaptic Connections in
            the Developing Cerebellum
  JOURNAL   Cell Rep 31 (9), 107703 (2020)
   PUBMED   32492419
REFERENCE   3  (bases 1 to 8777)
  AUTHORS   Kinoshita-Kawada,M., Hasegawa,H., Hongu,T., Yanagi,S., Kanaho,Y.,
            Masai,I., Mishima,T., Chen,X., Tsuboi,Y., Rao,Y., Yuasa-Kawada,J.
            and Wu,J.Y.
  TITLE     A crucial role for Arf6 in the response of commissural axons to
            Slit
  JOURNAL   Development 146 (3) (2019)
   PUBMED   30674481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8777)
  AUTHORS   Zhang,J., Neal,J., Lian,G., Hu,J., Lu,J. and Sheen,V.
  TITLE     Filamin A regulates neuronal migration through brefeldin
            A-inhibited guanine exchange factor 2-dependent Arf1 activation
  JOURNAL   J Neurosci 33 (40), 15735-15746 (2013)
   PUBMED   24089482
  REMARK    GeneRIF: coordinated interactions between actin (through FlnA) and
            vesicle trafficking (through Big2-Arf) direct the assembly and
            disassembly of membrane protein complexes required for neuronal
            migration and neuroependymal integrity.
REFERENCE   5  (bases 1 to 8777)
  AUTHORS   Lowery,J., Szul,T., Styers,M., Holloway,Z., Oorschot,V.,
            Klumperman,J. and Sztul,E.
  TITLE     The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane
            recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to
            the trans-Golgi network (TGN)
  JOURNAL   J Biol Chem 288 (16), 11532-11545 (2013)
   PUBMED   23386609
  REMARK    GeneRIF: an early acting GEF (GBF1) activates ARFs that mediate
            recruitment of late acting GEFs (BIG1/2) to coordinate coating
            events within the pre-Golgi/Golgi/TGN continuum.
REFERENCE   6  (bases 1 to 8777)
  AUTHORS   Grzmil,P., Enkhbaatar,Z., Gundsambuu,B., Oidovsambuu,O., Yalcin,S.,
            Wolf,S., Engel,W. and Neesen,J.
  TITLE     Early embryonic lethality in gene trap mice with disruption of the
            Arfgef2 gene
  JOURNAL   Int J Dev Biol 54 (8-9), 1259-1266 (2010)
   PUBMED   20857375
  REMARK    GeneRIF: Results indicate that murine BIG2 is essential for early
            embryonic development.
REFERENCE   7  (bases 1 to 8777)
  AUTHORS   Adham,I.M., Khulan,J., Held,T., Schmidt,B., Meyer,B.I.,
            Meinhardt,A. and Engel,W.
  TITLE     Fas-associated factor (FAF1) is required for the early
            cleavage-stages of mouse embryo
  JOURNAL   Mol Hum Reprod 14 (4), 207-213 (2008)
   PUBMED   18303090
REFERENCE   8  (bases 1 to 8777)
  AUTHORS   Lu,J., Tiao,G., Folkerth,R., Hecht,J., Walsh,C. and Sheen,V.
  TITLE     Overlapping expression of ARFGEF2 and Filamin A in the
            neuroependymal lining of the lateral ventricles: insights into the
            cause of periventricular heterotopia
  JOURNAL   J Comp Neurol 494 (3), 476-484 (2006)
   PUBMED   16320251
REFERENCE   9  (bases 1 to 8777)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 8777)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591703.14 and AL591911.8.
            
            On Feb 13, 2008 this sequence version replaced NM_001085495.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC158012.1, SRR10223970.4754.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-255               AL591703.14        84346-84600
            256-286             AL591911.8         18612-18642
            287-410             AL591911.8         19227-19350
            411-557             AL591911.8         26156-26302
            558-737             AL591911.8         27298-27477
            738-993             AL591911.8         27887-28142
            994-1062            AL591911.8         34370-34438
            1063-1214           AL591911.8         37135-37286
            1215-1345           AL591911.8         40552-40682
            1346-1580           AL591911.8         42728-42962
            1581-1680           AL591911.8         43640-43739
            1681-1820           AL591911.8         44940-45079
            1821-1929           AL591911.8         46590-46698
            1930-2113           AL591911.8         48092-48275
            2114-2225           AL591911.8         50948-51059
            2226-2431           AL591911.8         51471-51676
            2432-2516           AL591911.8         51999-52083
            2517-2688           AL591911.8         52210-52381
            2689-2840           AL591911.8         53269-53420
            2841-2969           AL591911.8         55242-55370
            2970-3128           AL591911.8         56378-56536
            3129-3276           AL591911.8         57365-57512
            3277-3376           AL591911.8         58597-58696
            3377-3417           AL591911.8         58831-58871
            3418-3587           AL591911.8         58973-59142
            3588-3739           AL591911.8         60577-60728
            3740-3912           AL591911.8         61895-62067
            3913-4073           AL591911.8         63144-63304
            4074-4204           AL591911.8         65261-65391
            4205-4334           AL591911.8         65548-65677
            4335-4470           AL591911.8         68178-68313
            4471-4609           AL591911.8         69956-70094
            4610-4664           AL591911.8         70203-70257
            4665-4779           AL591911.8         72857-72971
            4780-4910           AL591911.8         77464-77594
            4911-5079           AL591911.8         81954-82122
            5080-5218           AL591911.8         83452-83590
            5219-5336           AL591911.8         85186-85303
            5337-8777           AL591911.8         86297-89737
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.22 cM"
     gene            1..8777
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /note="ARF guanine nucleotide exchange factor 2"
                     /db_xref="GeneID:99371"
                     /db_xref="MGI:MGI:2139354"
     exon            1..255
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..5513
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /note="brefeldin A-inhibited GEP 2; ADP-ribosylation
                     factor guanine nucleotide-exchange factor 2 (brefeldin
                     A-inhibited); ADP ribosylation factor guanine nucleotide
                     exchange factor 2"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 2"
                     /protein_id="NP_001078964.1"
                     /db_xref="CCDS:CCDS38335.1"
                     /db_xref="GeneID:99371"
                     /db_xref="MGI:MGI:2139354"
                     /translation="MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIK
                     AELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITG
                     NAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTIL
                     QTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPMQSKPQS
                     PVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGS
                     PVSGRAEPSRGTDSGAQEVVKDILEDVVTSAVKEAAEKHGLPEPDRALGALECQECAV
                     PPGVDENSQTNGIADDRQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLS
                     MKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKN
                     GVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQ
                     TLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSL
                     RKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTS
                     VESTVSSGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAV
                     EDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGF
                     RLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNK
                     MTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETKEHTIATKSTKQSVASE
                     KQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSI
                     GLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQ
                     KNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREG
                     SLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTR
                     LDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIG
                     DHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSP
                     TIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTI
                     FQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTS
                     DDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWW
                     QDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVF
                     AQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLL
                     TWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYAHQKL
                     LASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMY
                     KFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLAC
                     CLRILFRMYVDENRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTK
                     TLKISDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQV
                     PAALSSTW"
     exon            256..286
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            287..410
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            411..557
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            558..737
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            738..993
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1062
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1214
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1345
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1580
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1680
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1820
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1929
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2113
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2225
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2431
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2516
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2688
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2840
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2969
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3128
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3276
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3376
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3377..3417
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3587
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3588..3739
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3740..3912
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3913..4073
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4074..4204
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4205..4334
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4335..4470
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4471..4609
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4610..4664
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4665..4779
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4780..4910
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4911..5079
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5080..5218
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5219..5336
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5337..8777
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8757..8762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      8777
                     /gene="Arfgef2"
                     /gene_synonym="BIG2; E230011G24Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggggcgtctc ttcctgacag ggcggcgcgc acggacgcgg ccggtgccgg ccgggtcgcc
       61 gggccctcag cctcgctctc ctgctcgctc gcaccgccgg ctcgcggggt cgccggcccc
      121 cgcaggccgg ggccatgcag gaaagccaga ccaagagcat gttcgtgtcc cgggccctgg
      181 agaagatcct agctgacaag gaggtgaagc ggccccagca ctcccagctg cgtagggcct
      241 gccaggtggc gctcgatgaa attaaagcag aactagaaaa gcagaggctt ggcgctgcgg
      301 ctccaccaaa ggcaaacttc attgaagctg acaagtattt ccttccattc gagctggcct
      361 gccaatccaa gtcacctcgg gtggtcagca catccctgga ctgcttgcag aaactcatcg
      421 catatggaca catcactggc aatgcccctg acagtggcgc tcctgggaag cggctgatcg
      481 acaggatcgt tgaaaccatt tgcaattgtt ttcagggccc tcagacagat gaaggggttc
      541 agctacaaat cattaaggct ctgctgactg ctgtgacatc cccacacatt gaaatccatg
      601 agggcaccat cctgcagaca gtgaggacct gctacaatat ctatctggcc agcaaaaacc
      661 tcatcaatca aaccaccgcc aaggccaccc ttacccaaat gctgaatgtc atcttcaccc
      721 gcatggaaaa ccaagtgtta caggaggcca gagaattgga aaaaccaatg cagtcaaaac
      781 ctcagtcccc ggtgatccag gccacagcag gctctcccaa gttcagccgc ctgaagcaga
      841 gccaggccca gagcaaaccc acgactcctg agaaagcaga gctgcccaat ggggaccatg
      901 cccagagtgg cctaggaaaa gtgagcttgg aaaatggaga agctccccga gagagaggct
      961 cgccagtctc tgggagagcc gagccttcga ggggcactga cagtggagct caggaggtgg
     1021 tgaaggacat cttagaggac gtggtcacgt ctgctgtgaa agaagcagca gaaaagcatg
     1081 gtctgccgga gcccgaccga gccctgggtg ccctagagtg ccaggaatgc gctgttcctc
     1141 ctggagttga tgaaaactca cagaccaacg gcatagcaga cgacaggcag tcgctatcct
     1201 ccgcagataa tctggaacca gatgttcaag gacaccaagt ggctgctagg ttctctcaca
     1261 ttctacagaa ggatgccttc ctggtgttcc gctccctgtg caagctgtcc atgaagcccc
     1321 ttggggaagg gccaccagat ccaaagtccc acgagcttcg ttccaaggtg gtttccctgc
     1381 agctgctcct ctctgtgctg cagaatgctg gcccagtctt caggagccac gagatgttcg
     1441 tcactgccat taagcagtac ctgtgtgtgg ccctttccaa gaatggcgtg tcctcagtgc
     1501 ctgacgtctt cgaactctcg cttgctattt ttcttactct tctttcaaac ttcaagatgc
     1561 acttgaaaat gcagatagag gtctttttca aagagatttt cctgaacatt ttagaaacat
     1621 ccacaagttc ttttgagcac cgctggatgg tcattcagac cctgacgagg atttgtgcag
     1681 atgcccagtg cgttgtggat atttatgtca actacgactg tgacttaaat gctgctaaca
     1741 ttttcgagcg actagtaaat gacttatcca aaattgctca gggaagaagt ggacatgagc
     1801 tggggatgac acctctacag gagctcagtc tgaggaagaa aggcctggag tgtctggtgt
     1861 ctattctcaa gtgcatggtg gagtggagca aggacctgta tgtgaatccc aaccaccagg
     1921 ctaccctggg tcaggagagg ctcccagatc aggaaatggg ggacggaaaa ggccttgaca
     1981 tggcaagacg gtgcagcgtg acatccgtgg agtccacagt gtcctcgggg acccagacag
     2041 ccattcagga tgacccagag cagtttgagg tcatcaaaca acagaaggaa atcattgagc
     2101 atggcattga gctcttcaat aagaagccca agcgaggtat ccagtttctc caagagcaag
     2161 gcatgctggg agcagctgta gaagatattg cccaatttct gcaccaggag gagcggctgg
     2221 attctaccca ggttggcgag tttctgggag acagcacgag gtttaacaag gaggtgatgt
     2281 atgcctatgt ggaccaactg gatttctgtg aaaaagagtt cgtctcagcc ctgaggacat
     2341 tcctggaggg cttccgcctg cctggggaag cccagaagat tgaccggtta atggagaagt
     2401 ttgctgcacg atacatagaa tgtaaccaag ggcaaaccct gtttgctagt gcggacactg
     2461 cgtatgtcct ggcgtactcc atcatcatgc tgaccacaga cctgcacagc ccacaggtaa
     2521 agaataagat gacaaaagag cagtatatta aaatgaaccg gggcatcaac gacagcaaag
     2581 acctgcctga ggagtacctg tccagcatct atgacgagat agagggcaag aagatagcga
     2641 tgaaggagac aaaggagcac acaattgcga ccaagtccac caagcagagt gtagctagtg
     2701 aaaagcagag gcggctgctg tacaatgtgg agatggagca gatggctaaa acagccaaag
     2761 ctctgatgga ggccgtgagc catgccaaag ccccgtttac cagcgccaca cacttggacc
     2821 atgtccggcc aatgttcaaa ctggtgtgga cgccgctgct ggcagcctat agtattggcc
     2881 tgcagaactg tgatgacacg gaggtggcct ccctgtgttt ggaaggcatc aggtgtgcag
     2941 tccgcattgc ctgcatcttt ggaatgcagc tggaacgaga tgcctatgtt caggcccttg
     3001 ctcgcttttc cctgctgaca gccagctcca gcatcacaga aatgaagcag aaaaacatcg
     3061 acaccataaa gacactcatc actgtggctc acaccgatgg caactacctt ggcaactcct
     3121 ggcatgagat cttgaagtgc atcagccagc tggagcttgc tcagctgata ggaactgggg
     3181 tgaagactcg ctacctctct ggctctgggc gggaacgaga agggagcctg aagggccaca
     3241 gtctggcagg agaagaattc atgggtcttg gcctcggtaa cttggtgagt ggtggtgtag
     3301 acaaaagaca gatggccagc ttccaggagt cggttggtga aacaagctca cagagtgtgg
     3361 ttgtcgcagt tgacagaatt tttactggct ctaccagact ggatggaaat gcaatagttg
     3421 actttgtccg ttggctgtgt gctgtgtcca tggatgagct ggcttctccc caccatcctc
     3481 gaatgttcag cctgcagaag attgtggaaa tatcatacta caacatgaac aggatccggc
     3541 tgcagtggtc tcggatatgg catgtcattg gagatcactt caataaggtc ggctgtaacc
     3601 ccaatgaaga tgtggccatc ttcgcagttg actctctgcg gcagctctcc atgaagttcc
     3661 tggagaaggg agaattagcc aacttccgat ttcagaagga ttttctgagg ccatttgaac
     3721 atattatgaa gaaaaacagg tccccgacca tccgggacat ggtgatccga tgcatcgctc
     3781 agatggtgag ctcccaggca gccaacatcc gctcaggctg gaagaacatc tttgctgtgt
     3841 tccaccaggc tgcctctgac cacgatggga acattgtgga gctggccttc caaaccacag
     3901 gccacatcgt ctcaaccatc ttccagcacc acttccctgc agccatcgac tccttccagg
     3961 atgctgtgaa gtgcttgtct gagttcgcct gcaatgcagc tttccctgac accagcatgg
     4021 aggccatccg gctcatccgc ttctgtggga aatacgtctc agagaggcct cgggtgctgc
     4081 aagagtacac aagtgatgat atgaatgtgg ctcctggtga cagggtctgg gtcagaggct
     4141 ggtttcccat cctgtttgaa ctctcctgca tcattaacag atgcaagtta gatgtacgca
     4201 caaggggact cacggtcatg tttgagatta tgaagagcta tggccacacc tttgcaaagc
     4261 actggtggca ggacctgttc agaatcgtgt ttcgaatttt tgacaacatg aaactccctg
     4321 agcaacagtc agagaaatca gagtggatga cgaccacgtg caatcatgca ctgtacgcta
     4381 tttgtgacgt attcacccag ttctacgaag ctttgcatga agtgcttctc tctgatgtgt
     4441 ttgcgcagct gcagtggtgc gtgaaacaag ataatgagca gttggcccga tcaggtacga
     4501 actgcttaga gaacctagtg atatccaacg gcgagaagtt cagccctgct gtctgggatg
     4561 aaacctgcaa ttgcatgttg gatattttca aaaccaccat cccacatgtt ttgctgacgt
     4621 ggagacctgc ggggatggag gaggaggtgt cagatagaca tctggatgtg gacctagacc
     4681 gccagtcttt aagcagcata gacagaaacg cctccgagag aggacagagc cagctgtcca
     4741 accccactga cgacagctgg aagggggcac cgtatgcaca tcagaaactg ctggccagcc
     4801 tcctcatcaa gtgtgtggtt cagctggagc tgatccagac cattgacaac atcgtgttct
     4861 accctgccac cagcaagaag gaggatgcgg agcacatggt tgctgcccag caagacaccc
     4921 tcgatgcaga gatccacatc gagacggaga atcagggcat gtacaagttc atgtcctccc
     4981 agcacctctt caagctgctg gactgcctgc aggagtccca ctccttctcc aaagccttca
     5041 actccaacta tgagcagcgg actgtcctct ggcgagctgg cttcaagggc aagtccaaac
     5101 ccaatcttct caaacaagaa accagcagcc tggcctgttg tctaaggatc ctgtttagaa
     5161 tgtacgttga tgagaaccgc agggattcct gggacgaaat acagcagcga cttctaagag
     5221 tgtgcagtga agcacttgcc tatttcatta ctgtgaactc cgagagccat cgggaagcat
     5281 ggacgagcct cctgctgcta cttctaacca aaaccctcaa gataagcgat gaaaagttca
     5341 aagcacacgc gtcaatgtac tacccctacc tgtgtgaaat tatgcagttt gacctgatcc
     5401 ctgagctccg agcagttctg cgaaagttct tcctgcggat aggcctggtg tataagatat
     5461 ggattccaga agagccgtcg caagtgccag cagcactgtc atcgacctgg tagcactggc
     5521 tgcatggcca ctgctgtggt tctgtagaac atccgccatg ccgtcccagc tggcggaggg
     5581 ggagctgccc acctagctgg gtttggacct ggagtgcagt ggcgtgaacc ctggtcagca
     5641 cagtccccag cggaggctca tggtgtctca ttaaaccatc acataccagt aactcggtgg
     5701 gcagggagtc tgttcccccg tccttgcatt ccgctggctg acctgttaga cctgtccttg
     5761 tgaatgccac cgtttcctct aagactggcc attaatgttt cgtcggcgga agtcgctctc
     5821 ctgggaggca atgagcattg ccgtataggt tcctgtttat tagagagtat gctaataaaa
     5881 gtccttgtaa tagctcacag ccacctaagt gcgcggtgtt ttgttgtttg actgtgttag
     5941 agaagtttga atgtttgtgt cagttttggg tcagcctaga taaagatggg aaagagggag
     6001 tgttccttat gtcttgtttt gtaattgttt gagtggtttc taaaatattt tacaaagata
     6061 tattattaaa aatttgatta aaggcagagt ttagtagact taagagcttg caaggtttta
     6121 agaaaatgta gtgcctcgtg gctccctggt ttagtaactt ggagagacag tctgtaggaa
     6181 aaagataatc taaaaaaagg tgttagcctt ccttgaagta tttgtgggtg gatgttccgt
     6241 taaattttcc agaatgcatt gcacatcttt ttatgaaatc tagactatga aaaggtgata
     6301 aacatgaagt aactgaccca gagttggttt atctttgagt aaaccttttt agtttttacc
     6361 tcagcagagt gttagtatgg ctgctttgca gtccctgcta aagggaccac cagagggagg
     6421 gacagttcgt acggccctct cagcacgcgt catgagtggg actggagccc ctgcgggact
     6481 agcacagctg ggctgtgaca gtggcagagg tggcacccag agcttctgtt aacttgagcc
     6541 gggagctggg ttctttagca agatgagctt ctggggtcct tatgttggta atggtgccat
     6601 gtccagaccc acagcagagt gaagtctgag tgctcctaac aacccacagc agccacgcta
     6661 gaagcttaat ggattcggag cccatgagag aaagaacaca ggcttcctgg tctgccctcc
     6721 ccctccgctc accaggagct aaactgagag gctaaactgt gtcatgaagc gcgttggaaa
     6781 cagacttctg cctacatcga gtcctacgaa ttggggttgt attgatgtca ctctggtgcc
     6841 ttagaagtca gtcgtccttg ggaaggtgtc tgcgagactg ggccctttct tctctccaga
     6901 gcaggatgcc tggtgctctt gctattctct ctctcgtttc tgacagcctt ggcacatgct
     6961 cggtttggcc agtagaattt gtatttatgc tttttttttc cccaaaagta ttttaatttc
     7021 tctaccaaag ggggaaaaga aagcaggctg aggttattat tgctaacaca cctcagccga
     7081 gcaggctatg tacctcgaag ggagggcctg ggggacaatg ttaccgagag gagaatgtga
     7141 gcacatttga ctgacacggc ctggaaccca gtgcatcttt tcacggcact gccgagacgt
     7201 actccctggt gttgccgctc acaggccctc agctgtcggt ggttcctgca gcttttgttc
     7261 ctcgcatgta atcagggagc acgcatgcac gttgagtggg tgcatcctgc ctaagctgtg
     7321 tccctgagtg gtggctttcc ctcctgtcac gtgacatccc attcactcat ctacactagc
     7381 ccagtgctcc cattgtcccc tgcctcctct gtaatttttg ccccttccag ggtacggaat
     7441 gtgtcagtgc attttctcct tgctgagagt ctcagctcca atctcttcat aattttgttc
     7501 tgtaaaagat gaacctttcc gagtgatgca ttcaaactgt ctagcaatag atcttttaaa
     7561 acgatgtaaa accattcagg gtaatttttc ttgtcagtgg ccagtggcat ccctgacctc
     7621 cagaaagaag ccatgcagta taaactcctt gtggtgaaca agtctgttca tggcaaacgt
     7681 ggctcgagcc caccagacac cagcgcagag cgggggcagc ctgccccgct gtagcacagg
     7741 agcaatggtg tagcgtcttc caaggcctta cttttttttc agaatgcttt aagtgttggc
     7801 tgtatgtgct gggttctcta aagaagtttc tcttcccgag ctgtttttcc gagcagtttt
     7861 gttcgtgtct cttaaggaaa tcaccagctt tggcatctga ccaacaaaca gtgagtggga
     7921 gacgtgattt ccgatgcgca tcttcagagt caaacacacc ctccctcgtg tacaaccaaa
     7981 gaacaggcat tatgaaattg tatagtaata tctagaaaca ccacttccct ccaacctgcg
     8041 acttttgtac aaaattcaga gaatctgtaa agaacccccc acccctcaaa aaagcctaag
     8101 cacttgctgt tgttcttttg tgattatggc tgtacattta gttgtctttc tagttatgaa
     8161 aagaacgttg acacacgtag gcatggccgc cccactcagg tgttcgttgt tacagtgcta
     8221 atggatcctg ggtgcagtag gcgctgggtc ctcctgacct ctgtttcact gtttgtggta
     8281 aggtagccac acctggagct cacgtgtaac ctggagcgct gtgctaccag tagctttggg
     8341 gttttggaag cagacacacc aaagccgagc atcatagaga atgaatccct ctggaaacac
     8401 ttactctcag aaaatgacac tcttcccaca tgtggggttt tctcagtgtt ctaagttttt
     8461 aagaagtttt atcttgtgta tttgcatcaa acatttcttt atgtgctaaa tgtttgctct
     8521 ttaaaaatgt ctcagagtta gttaccagct tccttgattt catctataca caatgattaa
     8581 gtgcatttaa tattggtaat ttaatgaaaa gcattccttg cccaatggat gtaaacattt
     8641 ttgaaagaat aaagcagaat tatataatat gaaatgctat gtaaagtttt ctatgtaaaa
     8701 aaatattatg tataatactg ttaaataccc catgctttga tcaggtggaa aaaatcaata
     8761 aagttttaaa aagtaaa
//
