LOCUS       NM_001102430            7042 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus ARF guanine nucleotide exchange factor 1 (Arfgef1),
            mRNA.
ACCESSION   NM_001102430 XM_975420
VERSION     NM_001102430.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Sun,M., Han,X., Zhou,D., Zhong,J., Liu,L., Wang,Y., Ni,J., Shen,X.,
            Liang,C. and Fang,H.
  TITLE     BIG1 mediates sepsis-induced lung injury by modulating lipid
            raft-dependent macrophage inflammatory responses
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 53 (8), 1088-1097 (2021)
   PUBMED   34153089
  REMARK    GeneRIF: BIG1 mediates sepsis-induced lung injury by modulating
            lipid raft-dependent macrophage inflammatory responses.
REFERENCE   3  (bases 1 to 7042)
  AUTHORS   Teoh,J., Subramanian,N., Pero,M.E., Bartolini,F., Amador,A.,
            Kanber,A., Williams,D., Petri,S., Yang,M., Allen,A.S., Beal,J.,
            Haut,S.R. and Frankel,W.N.
  TITLE     Arfgef1 haploinsufficiency in mice alters neuronal endosome
            composition and decreases membrane surface postsynaptic GABAA
            receptors
  JOURNAL   Neurobiol Dis 134, 104632 (2020)
   PUBMED   31678406
  REMARK    GeneRIF: Arfgef1 haploinsufficiency in mice alters neuronal
            endosome composition and decreases membrane surface postsynaptic
            GABAA receptors.
REFERENCE   4  (bases 1 to 7042)
  AUTHORS   Kinoshita-Kawada,M., Hasegawa,H., Hongu,T., Yanagi,S., Kanaho,Y.,
            Masai,I., Mishima,T., Chen,X., Tsuboi,Y., Rao,Y., Yuasa-Kawada,J.
            and Wu,J.Y.
  TITLE     A crucial role for Arf6 in the response of commissural axons to
            Slit
  JOURNAL   Development 146 (3) (2019)
   PUBMED   30674481
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7042)
  AUTHORS   Miyamoto,Y., Torii,T., Tago,K., Tanoue,A., Takashima,S. and
            Yamauchi,J.
  TITLE     BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by
            Schwann cells in mice
  JOURNAL   Sci Adv 4 (4), eaar4471 (2018)
   PUBMED   29740613
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7042)
  AUTHORS   Steinmetz,C.C., Tatavarty,V., Sugino,K., Shima,Y., Joseph,A.,
            Lin,H., Rutlin,M., Lambo,M., Hempel,C.M., Okaty,B.W., Paradis,S.,
            Nelson,S.B. and Turrigiano,G.G.
  TITLE     Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting
            AMPAR into the Recycling Endosomal Pathway
  JOURNAL   Cell Rep 16 (10), 2711-2722 (2016)
   PUBMED   27568566
REFERENCE   7  (bases 1 to 7042)
  AUTHORS   Caronia-Brown,G., Yoshida,M., Gulden,F., Assimacopoulos,S. and
            Grove,E.A.
  TITLE     The cortical hem regulates the size and patterning of neocortex
  JOURNAL   Development 141 (14), 2855-2865 (2014)
   PUBMED   24948604
REFERENCE   8  (bases 1 to 7042)
  AUTHORS   Lowery,J., Szul,T., Styers,M., Holloway,Z., Oorschot,V.,
            Klumperman,J. and Sztul,E.
  TITLE     The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane
            recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to
            the trans-Golgi network (TGN)
  JOURNAL   J Biol Chem 288 (16), 11532-11545 (2013)
   PUBMED   23386609
  REMARK    GeneRIF: an early acting GEF (GBF1) activates ARFs that mediate
            recruitment of late acting GEFs (BIG1/2) to coordinate coating
            events within the pre-Golgi/Golgi/TGN continuum.
REFERENCE   9  (bases 1 to 7042)
  AUTHORS   Imayoshi,I., Shimogori,T., Ohtsuka,T. and Kageyama,R.
  TITLE     Hes genes and neurogenin regulate non-neural versus neural fate
            specification in the dorsal telencephalic midline
  JOURNAL   Development 135 (15), 2531-2541 (2008)
   PUBMED   18579678
REFERENCE   10 (bases 1 to 7042)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC102462.19
            and AC159972.5.
            
            On Aug 23, 2007 this sequence version replaced XM_975420.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.39.1, SRR10223971.32.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-300               AC102462.19        62502-62801
            301-331             AC102462.19        78613-78643
            332-488             AC102462.19        79179-79335
            489-635             AC102462.19        80901-81047
            636-815             AC102462.19        81949-82128
            816-1092            AC102462.19        85471-85747
            1093-1194           AC102462.19        90263-90364
            1195-1370           AC102462.19        95268-95443
            1371-1504           AC102462.19        96196-96329
            1505-1739           AC102462.19        100681-100915
            1740-1979           AC102462.19        105494-105733
            1980-2088           AC102462.19        105837-105945
            2089-2290           AC102462.19        106214-106415
            2291-2402           AC102462.19        110675-110786
            2403-2608           AC102462.19        110991-111196
            2609-2693           AC102462.19        111425-111509
            2694-2865           AC102462.19        114131-114302
            2866-3017           AC102462.19        115195-115346
            3018-3146           AC102462.19        120977-121105
            3147-3305           AC102462.19        121820-121978
            3306-3456           AC102462.19        122188-122338
            3457-3547           AC102462.19        131385-131475
            3548-3588           AC102462.19        134169-134209
            3589-3758           AC102462.19        134302-134471
            3759-3910           AC102462.19        135144-135295
            3911-4083           AC102462.19        135432-135604
            4084-4244           AC102462.19        137112-137272
            4245-4375           AC102462.19        138128-138258
            4376-4505           AC102462.19        140762-140891
            4506-4641           AC159972.5         198514-198649       c
            4642-4780           AC159972.5         198293-198431       c
            4781-4853           AC159972.5         196708-196780       c
            4854-4986           AC159972.5         192310-192442       c
            4987-5126           AC159972.5         189625-189764       c
            5127-5295           AC159972.5         187483-187651       c
            5296-5434           AC159972.5         186466-186604       c
            5435-5552           AC159972.5         185374-185491       c
            5553-7042           AC159972.5         182414-183903       c
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.36 cM"
     gene            1..7042
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /note="ARF guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:211673"
                     /db_xref="MGI:MGI:2442988"
     exon            1..300
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            301..331
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            332..488
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            489..635
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            636..815
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            816..1092
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1194
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1370
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1504
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1739
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1979
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2088
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2290
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2402
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2608
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2693
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2694..2865
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..3017
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3146
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3305
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3306..3456
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3547
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3548..3588
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3589..3758
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3910
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            3911..4083
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4084..4244
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4245..4375
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4376..4505
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4641
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4642..4780
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4781..4853
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4854..4986
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            4987..5126
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5127..5295
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5296..5434
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5435..5552
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     exon            5553..7042
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6937..6942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /note="hexamer: AATAAA"
     polyA_site      6979
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /note="major polyA site"
     regulatory      7011..7016
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
                     /note="hexamer: ATTAAA"
     polyA_site      7034
                     /gene="Arfgef1"
                     /gene_synonym="ARFGEP1; BIG1; D130059B05Rik;
                     D730028O18Rik; P200"
ORIGIN      
        1 cggccgctcc gcgtcgccgg ccgcttcctg gtgccgccgc tgccctccac gcctcctcgc
       61 ccccaccgcc acctcctcgc ctttctccct ccccgtcctc atcccggtcc ccgtccccct
      121 cctccgcctt ccctccagcc gccgggaccg ccgccgccgc cgcctcctcc gcgtccatgt
      181 atgagggcaa gaagacgaag aacatgttcc tgacccgggc cctggagaag attctggccg
      241 acaaggaagt gaagaaggcg catcactccc agctgcgcaa agcgtgcgag gtggccctag
      301 aggaaataaa agttgaaact gagaaacaaa gccctcctca tggagaagca aaagctggat
      361 caggcaccct tccaccagtg aaatcaaaga caaattttat tgaagcagac aagtactttt
      421 taccctttga gttggcatgc cagtccaaat gtccccgcat agttagtaca tctctagatt
      481 gtttgcagaa acttattgct tatgggcact tgactggcag ggctccagat agtacgacac
      541 caggcaaaaa attaattgat agaattattg aaacaatatg tggctgcttc caaggtcctc
      601 aaacagatga aggagttcag ttgcagataa taaaggcttt acttaccgca gtgacatcac
      661 aacacataga aatccatgaa ggaactgtgc tgcaagctgt gaggacatgc tacaatatct
      721 atttagccag caaaaatctc atcaatcaga caacagccaa agctactctt actcaaatgc
      781 tgaacgttat ctttgcacgc atggaaaacc aagcattaca ggaggccaaa caaatggaaa
      841 gagaaagaca tcggcagcag cagcatttgt tacagtctcc agtaagccat catgagcccg
      901 agtcacctca tcttagatat ttgccacctc agactgttga tcatataaac caagagcatg
      961 aaggggatct tgagccccaa acacatgatg tggataaaag tctccaagat gatacagagc
     1021 ctgaaaacgg atctgatatt tccagtgcag aaaatgaaca gacagaagct gatcaggcaa
     1081 ctgcagctga aacattatct aaaaatgata tcttgtatga tggagactat gaagaaaagc
     1141 cacttgatat tgtccagagc attgtagaag aaatggtgaa catcattgtt ggagacatgg
     1201 gagaagggat ggctatcagt gcaagtacag aaggcaacac tggaactgta gaagatggta
     1261 gtgacagtga aaacatccaa gcaaatggaa tcccaggaac acccatttct gttgcatata
     1321 caccatcgtt acctgacgac aggttatctg tctcctccaa tgatactcag gaatctggaa
     1381 attcttcagg accttcacct ggtgctaagt tttcccacat tttacaaaag gatgcctttc
     1441 tagtgttcag gtcattgtgt aagctgtcaa tgaaaccact gtcagatgga ccaccagatc
     1501 caaagtctca tgagttacgg tccaagattc tttcattgca gttgcttctg tccattctgc
     1561 agaatgcagg acctgttttc aggacaaatg agatgtttat taatgctatc aagcagtatc
     1621 tatgtgttgc actctcaaaa aacggagtct catctgttcc agaggttttt gagctttctc
     1681 tttccatatt tcttactttg ttgtcaaact tcaagacaca tctgaagatg caaattgagg
     1741 tattctttaa agaaatcttc ctgtacattt tggaaacgtc tacaagctca tttgaccata
     1801 agtggatggt tattcagaca ctgacaagga tctgtgcaga tgctcagagt gtggtggaca
     1861 tttatgtaaa ttatgactgt gacctaaatg cagcaaatat atttgaaaga ctagtaaatg
     1921 acctgtcgaa aattgcccaa ggaaggggca gccaagaact tggtatgagt aatgttcagg
     1981 aattgagcct gcgaaaaaag gggttagaat gtttagtttc aattttgaaa tgtatggttg
     2041 aatggagcaa ggatcagtat gtgaacccca actctcaaac tacccttggt caggaaaaac
     2101 cctcagagca agagataagt gaagttaaac acccagagac tataaacaga tatggaagtt
     2161 taaactcctt agaatccaca tcatcatcag gaattggcag ctacagtaca cagatgtctg
     2221 gcactgataa tccagaacaa tttgaggtcc taaagcagca aaaagaaata atagaacaag
     2281 ggattgattt atttaataag aaaccaaaga gaggaataca atacctccaa gaacaaggga
     2341 tgcttggcac cacacccgaa gatatagctc agttcttaca tcaagaagaa agattagact
     2401 ctactcaggt gggtgagttc ctgggagata atgataaatt caataaagaa gtcatgtatg
     2461 catatgtaga ccaacatgac ttttcaggaa aagactttgt ttcagctctt cgtctatttc
     2521 tggaaggatt ccggcttcca ggagaagctc agaaaattga tcgattaatg gaaaaatttg
     2581 ctgcaagata cctagagtgc aaccaaggac aaaccctttt tgctagtgca gatactgctt
     2641 atgttttggc ctactcaatt atcatgttga ctacagacct tcacagtcca caggttaaaa
     2701 acaaaatgac aaaagaacag tacattaaaa tgaatcgtgg tatcaatgac agtaaagatc
     2761 tccctgaaga gtatctatca gccatttata acgaaatagc tgggaaaaaa atatcaatga
     2821 aagaaacaaa agaactaaca atccctacaa agtcaactaa acagaatgta gccagtgaaa
     2881 agcaaagacg acttctgtat aacttagaaa tggaacagat ggccaagacg gctaaagcac
     2941 tcatggaagc tgtgagccat gttcaggcac cttttacaag tgcaacacac ttggagcacg
     3001 tgaggccgat gtttaagttg gcttggacac cttttctggc tgcattcagt gtgggtctcc
     3061 aagactgtga tgatactgaa gtagcctctc tttgcctaga agggataaga tgtgctatca
     3121 ggattgcatg cattttcagc attcagctgg aaagagatgc atatgtccaa gcactagcca
     3181 gatttacctt acttacagtg agttctggta ttactgaaat gaaacagaag aatatcgaca
     3241 caataaaaac actcatcacg gtggctcaca ctgatggaaa ttatttagga aattcgtggc
     3301 atgagatcct gaagtgcatc agtcagttag agctggcaca acttatagga actggagtga
     3361 agcctcggta catttctggg actgtgagag gcagagaagg gtctctcact gggacaaaag
     3421 atcaggctcc agatgaattt gttggtctag ggctagttgg aggaaatgtg gactggaagc
     3481 agatagccag tattcaggag tctattggag aaaccagctc tcaaagtgtg gtagttgctg
     3541 tggatagaat attcaccgga tccacaaggc tagatggaaa tgctattgtt gattttgtcc
     3601 gttggctatg tgctgtgtct atggatgaat tgctttctac tacacaccca agaatgttta
     3661 gtctacaaaa aatagtagaa atatcatact ataacatggg aagaataaga ctgcagtggt
     3721 ctcggatttg ggaagttatt ggagatcatt ttaataaggt tggttgcaat cctaatgaag
     3781 atgtagccat atttgcagta gactctctga ggcagctatc aatgaagttt ttagagaaag
     3841 gagagcttgc aaacttcaga ttccaaaagg atttcttacg accttttgaa catataatga
     3901 aacgaaacag gtctccaaca attcgagata tggttgtacg gtgtatagca caaatggtta
     3961 attctcaggc tgcaaatatt cgttcaggat ggaagaacat tttctcagta ttccatctag
     4021 ctgcatcaga ccaagatgaa agcatagtag aacttgcatt tcagacaaca gggcacattg
     4081 tcactcttgt atttgaaaaa cactttccgg ccaccattga ttctttccag gatgcagtga
     4141 agtgtttgtc tgaattcgca tgtaatgcag ctttcccaga cacaagtatg gaagcaatcc
     4201 gacttattcg ccattgtgca aaatatgtgt ctgatagacc gcaggctttc aaggagtaca
     4261 caagtgatga catgagcgtg gcacctgaag acagggtgtg ggtgagaggg tggttcccga
     4321 tcctctttga gttgtcttgt atcatcaata gatgcaaatt agatgtaaga accaggggct
     4381 taacagtaat gtttgaaata atgaaaacat atggccacac ttatgaaaaa cattggtggc
     4441 aggacttatt cagaattgtt ttcagaatct ttgacaacat gaaattgcca gaacagcaga
     4501 cagagaaagc tgaatggatg acaacaactt gtaaccatgc cctttatgca atatgtgatg
     4561 tattcaccca gtatttagaa gttctcagcg atgtactttt ggatgatatt tttgcccagc
     4621 tttactggtg tgtacaacaa gacaatgagc agttagcacg atctggtaca aactgcttag
     4681 aaaacgtggt tattctgaat ggtgaaaagt ttactcttga aatctgggat aaaacgtgta
     4741 actgcaccct ggatatcttc aaaaccacaa ttcctcacgc gcttctgact tggcgtccca
     4801 cctctggcga ggccgaaccc ccatctccat cagctgtgag tgaaaagccg ttggatgcaa
     4861 tatcacaaaa atctgtagac atacatgatt ctattcaacc aagatcttca gataacagac
     4921 agcaagcacc actggtttct gtttctactg tgagtgaaga agttagcaaa gtgaaatcca
     4981 cagcaaaatt cccagaacaa aaattgtttg ctgccctgtt gattaaatgt gttgtgcagc
     5041 tggaactcat ccagaccatc gacaacattg tcttcttccc agccacaagc aagaaggagg
     5101 atgcagagaa cttagctgca gcccagagag atgcagtaga cttcgatgtt cgagttgata
     5161 ctcaagacca aggaatgtac cgctttttaa catcacaaca actgttcaag ctactggact
     5221 gcttattaga gtcgcataga tttgcaaaag catttaattc caacaatgaa cagaggactg
     5281 ctctgtggaa agcaggcttc aaaggcaagt ccaagcccaa tcttctaaaa caggagacaa
     5341 gcagcctggc gtgtgggctg cgcattctct tccggatgta catggacgag agcagagtca
     5401 gtgcctggga agaagtccag cagaggcttt taaacgtctg cagggaagcg ctgagctact
     5461 tcctcactct aacatccgaa agtcaccggg aagcctggac taacttactg ttactgttcc
     5521 tcactaaagt tctgaagatc agtgacagca ggtttaaagc tcacgcatca ttctactacc
     5581 ctctcttatg tgaaattatg caatttgact tgattccgga actgcgtgct gttcttagaa
     5641 gatttttcct gcgaattgga atagtatttc aaatatcaca accacctgaa caggaacttg
     5701 ggataaacag gcaatgatga gagccgatgt ttttctgttg gtgtttacat tgctctgctc
     5761 tgtgaaagga tcctgcagca gaggcttcct acctgaaaaa tgtttctctc aattgtagtg
     5821 tgtgagttac tggtaagacg cttacaccta taactcaagg tcggaatagt ccctccagtc
     5881 cctttagtaa cccagtggcc tctgtctcgt gttgggctca tgttgagcag aaaggctgtt
     5941 cccacagcat cagcctacct ttagggtcct tccctgtcct ctaagcaggg tgagcccaca
     6001 ctgtggcagg tacggaaatt acccaaagaa gtatagatgt aaagactgag cttctgcgag
     6061 aagtacaact caggagagct gtattttgaa ggataaaatg tttataattg ggggtggggg
     6121 aagggagaaa aaaattattg ctcatggtaa aagatattta gcaactatgg tattcttact
     6181 ctgaagattt ttgcacactc aggctatctg agacaccgga aatcatcatc tttctgtgaa
     6241 aatgtacaga gatgcaggtc tgtaatataa aaatcttaaa catattgttc ctcctgcact
     6301 gttttatttc tttattttct tatctacttg ctaaataccc ataatatttt gtcaaatgca
     6361 ctaaaacatt tgggttgaac ttttagtctt tgggggagtg gtggtcattt tgagggcttg
     6421 ctgtgccctg aatctgttgt ggctgatgcc tgtcaagatg acaacaggag gggatccacc
     6481 aaggtagaaa acatccctgc tgacaagacc tacctgtgac atactcactc tcttagggct
     6541 tttaaagaca agccattaaa atagatgacc tttcacagac tgaaacttga atcactgcaa
     6601 aatgaatcta ggttgctgtc actttttctt ttgggtggcg gggcttgatg tagattttat
     6661 tctatgtaca gaatttaatg ttgaatatat taaaaataac aaacctggca tggttttggg
     6721 aggttagatt tactggaaat gtattcatac tgtgaactgt gctctgatgg ttagaatgaa
     6781 aagcttatca agcattccat ataacagtgg atgtagaaaa tgttttcagg tggatatgta
     6841 tatggtacag atgtaaagtt ttctatgtaa aaaattctgt acaactttct gtacaatatt
     6901 gattctcatc tggcatatct aatcaggtta taggtcaata aagtttttga attatttcat
     6961 cagtgcagtg taacccacag ccctgttttc attgtttttt gtttgtttta attaaaggtc
     7021 aaaccatgaa aagatttttt tt
//
