LOCUS       NM_009703               2405 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus Araf proto-oncogene, serine/threonine kinase (Araf),
            transcript variant 1, mRNA.
ACCESSION   NM_009703
VERSION     NM_009703.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Su,D., Zhu,S., Hou,Z., Hao,F., Xu,K., Xu,F., Zhu,Y., Liu,D., Xu,J.
            and Tao,J.
  TITLE     Toxoplasma gondii infection regulates apoptosis of host cells via
            miR-185/ARAF axis
  JOURNAL   Parasit Vectors 16 (1), 371 (2023)
   PUBMED   37858158
  REMARK    GeneRIF: Toxoplasma gondii infection regulates apoptosis of host
            cells via miR-185/ARAF axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2405)
  AUTHORS   Takahashi,K., Itakura,E., Takano,K. and Endo,T.
  TITLE     DA-Raf, a dominant-negative regulator of the Ras-ERK pathway, is
            essential for skeletal myocyte differentiation including myoblast
            fusion and apoptosis
  JOURNAL   Exp Cell Res 376 (2), 168-180 (2019)
   PUBMED   30742807
  REMARK    GeneRIF: DA-Raf1 (DA-Raf) is a splicing variant of A-Raf that
            suppresses the Ras-ERK pathway and is essential for both myocyte
            differentiation including myoblast fusion and apoptosis but
            apoptosis is not a prerequisite for myoblast fusion
REFERENCE   4  (bases 1 to 2405)
  AUTHORS   Horiuchi,Y., Nakatsu,D., Kano,F. and Murata,M.
  TITLE     Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic
            reticulum stress-induced apoptosis by activating MEK1/ERK pathway
            in mouse insulinoma cells
  JOURNAL   Cell Signal 38, 212-222 (2017)
   PUBMED   28743549
  REMARK    GeneRIF: PKM1 interacts with A-Raf, an upstream regulator of the
            MEK/ERK pathway, and that this interaction contributes to MEK1
            phosphorylation by A-Raf.
REFERENCE   5  (bases 1 to 2405)
  AUTHORS   Papaioannou,G., Petit,E.T., Liu,E.S., Baccarini,M., Pritchard,C.
            and Demay,M.B.
  TITLE     Raf Kinases Are Essential for Phosphate Induction of ERK1/2
            Phosphorylation in Hypertrophic Chondrocytes and Normal
            Endochondral Bone Development
  JOURNAL   J Biol Chem 292 (8), 3164-3171 (2017)
   PUBMED   28073913
  REMARK    GeneRIF: A- and B-Raf ablation in chondrocytes does not alter
            skeletal development, whereas ablation of C-Raf decreases
            hypertrophic chondrocyte apoptosis and impairs vascularization of
            the growth plate. However, ablation of C-Raf does not impair
            phosphate-induced ERK1/2 phosphorylation in vitro, but leads to
            rickets by decreasing VEGF protein stability.
REFERENCE   6  (bases 1 to 2405)
  AUTHORS   Derry,J.M. and Barnard,P.J.
  TITLE     Physical linkage of the A-raf-1, properdin, synapsin I, and TIMP
            genes on the human and mouse X chromosomes
  JOURNAL   Genomics 12 (4), 632-638 (1992)
   PUBMED   1572636
REFERENCE   7  (bases 1 to 2405)
  AUTHORS   Grant,S.G. and Chapman,V.M.
  TITLE     Detailed genetic mapping of the A-raf proto-oncogene on the mouse X
            chromosome
  JOURNAL   Oncogene 6 (3), 397-402 (1991)
   PUBMED   2011396
REFERENCE   8  (bases 1 to 2405)
  AUTHORS   Storm,S.M., Cleveland,J.L. and Rapp,U.R.
  TITLE     Expression of raf family proto-oncogenes in normal mouse tissues
  JOURNAL   Oncogene 5 (3), 345-351 (1990)
   PUBMED   1690378
REFERENCE   9  (bases 1 to 2405)
  AUTHORS   Avner,P., Bucan,M., Arnaud,D., Lehrach,H. and Rapp,U.
  TITLE     A-raf oncogene localizes on mouse X chromosome to region some 10-17
            centimorgans proximal to hypoxanthine phosphoribosyltransferase
            gene
  JOURNAL   Somat Cell Mol Genet 13 (3), 267-272 (1987)
   PUBMED   3299748
REFERENCE   10 (bases 1 to 2405)
  AUTHORS   Huleihel,M., Goldsborough,M., Cleveland,J., Gunnell,M., Bonner,T.
            and Rapp,U.R.
  TITLE     Characterization of murine A-raf, a new oncogene related to the
            v-raf oncogene
  JOURNAL   Mol Cell Biol 6 (7), 2655-2662 (1986)
   PUBMED   3491291
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB857915.1, AK085823.1 and AA960102.1.
            
            On Apr 25, 2009 this sequence version replaced NM_009703.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085823.1, BC004757.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                BB857915.1         1-35
            36-1535             AK085823.1         2-1501
            1536-1842           AK085823.1         1503-1809
            1843-2358           AK085823.1         1811-2326
            2359-2405           AA960102.1         1-47                c
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 16.3 cM"
     gene            1..2405
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /note="Araf proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:11836"
                     /db_xref="MGI:MGI:88065"
     exon            1..77
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            78..233
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..1952
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     raf-related oncogene; v-raf murine sarcoma 3611 viral
                     oncogene homolog 1; A-Raf proto-oncogene
                     serine/threonine-protein kinase; serine/threonine-protein
                     kinase A-Raf; proto-oncogene A-Raf"
                     /codon_start=1
                     /product="serine/threonine-protein kinase A-Raf isoform 1"
                     /protein_id="NP_033833.1"
                     /db_xref="CCDS:CCDS40886.1"
                     /db_xref="GeneID:11836"
                     /db_xref="MGI:MGI:88065"
                     /translation="MEPPRGPPVSGAEPSRAVGTVKVYLPNKQRTVVTVREGMSVYDS
                     LDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFV
                     RKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRRQFYHSIQD
                     LSGGSRQQEAPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRIRSTSTPNV
                     HMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPSEQ
                     RERKSLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVK
                     VLKVAQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHL
                     HVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLAT
                     VKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYS
                     HIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQILATIE
                     LLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP"
     exon            234..337
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            338..440
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            441..595
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            596..694
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            695..836
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            837..864
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1004
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1207
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1384
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1431
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1550
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1682
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1817
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..2360
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2229
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /note="major polyA site"
     polyA_site      2358
                     /gene="Araf"
                     /gene_synonym="1200013E08Rik; A-Raf; Araf1"
                     /note="major polyA site"
ORIGIN      
        1 gacggtggcg gtggactgag aggaaacaag aagacaagcc caagatggag gcggtggtgg
       61 tggtagcggt gtgacaggag gcctatggca gctgcccagt cctacctcag cccatcttga
      121 aaacaatctt aggcaacatg gaaccaccac gaggccctcc tgttagtggg gctgagccat
      181 ctcgggcagt tggcactgtc aaagtgtacc tgcctaacaa gcaacgcaca gtggtgactg
      241 tccgggaagg catgagtgtc tatgactctt tggacaaggc cctgaaggtg cggggtctca
      301 accaggactg ctgtgtggtc tacaggctca tcaaaggaag aaagacagtc actgcctggg
      361 acacagccat tgcgcctctg gatggtgagg agctcattgt ggaggtccta gaagatgtgc
      421 cactgaccat gcacaatttt gtacggaaga cattcttcag cctggccttc tgtgacttct
      481 gccttaagtt tctgttccat ggattccgct gccagacctg tggctacaag ttccaccagc
      541 attgttcctc caaggtcccc accgtctgcg ttgacatgag taccaaccgc cgacagttct
      601 accacagcat ccaggatctg tctggaggct ccaggcagca ggaggctccc tcaaatctct
      661 ctgtgaatga gctgctaacc ccccagggtc ccagcccctt tacccagcaa cgtgaccagg
      721 agcacttctc attccctgcc cctgccaatc ccccactgca gcgcatccgc tccacatcta
      781 ctcctaacgt ccacatggtc agcaccacag ctcccatgga ctccagcctc atgcagttta
      841 ctgctcagag cttcagcacc gatgctgctg gtagaggtgg tgatggagcc cctcggggta
      901 gccctagccc agctagtgtg tcttcaggga ggaagtcccc acattccaag ttaccttcag
      961 aacagcggga gcggaagtcc ttggcagatg aaaagaaaaa agtgaagaac ctggggtacc
     1021 gggactcagg ctattactgg gaggtgccac ccagtgaggt acagctgttg aagaggatcg
     1081 ggacaggctc ttttggcact gtgtttcggg ggcgttggca tggcgatgta gctgtgaaag
     1141 tgctcaaggt ggcccaacct accgctgagc aggcccaggc cttcaagaat gagatgcagg
     1201 tgctcaggaa gacacggcat gtcaacattt tgctgttcat gggtttcatg actcggccgg
     1261 ggtttgccat catcacacag tggtgtgagg gttccagcct ctaccaccac ctacatgtgg
     1321 ctgacacgcg ctttgacatg gtccagctca ttgatgtggc ccggcagact gcccagggca
     1381 tggactacct ccacgccaag aacatcattc atcgagacct aaagtccaac aatatcttct
     1441 tacatgaggg gctcacagtc aagattggtg acttcggcct ggccacagtg aagacacggt
     1501 ggagtggggc acagccctta gagcagccct cagggtctgt gctatggatg gcagctgagg
     1561 tgatccgtat gcaggacccg aacccctaca gcttccagtc ggatgtctat gcctatggtg
     1621 ttgtgctcta tgagctcatg accggctcac tgccctacag ccacattggc agccgtgacc
     1681 agatcatctt tatggtgggc cgtggctatc tgtctccgga cctcagcaaa atcttcagta
     1741 attgccccaa agccatgagg cgcctgctga ctgactgcct caagttccag cgggaggagc
     1801 ggcccctatt tccccagatt ctggccacga tcgagctact gcagcggtca ctccccaaga
     1861 ttgagcggag tgcctccgaa ccctccttgc accgcaccca ggctgatgag ttgcctgctt
     1921 gccttctcag cgcagcccgt cttgtgcctt agacctcact cccagcccac cagggagcca
     1981 atttcagcat accatgccaa ggcacaccga cctaccagcc aataactgtt ctgcctctgc
     2041 cttgatactg cctcaggatt ccctatccca taccctggga aatttgaggg accccaaaaa
     2101 ctgaggtctc ctgctttctc cataatttgg tctcctcttg ggtttgggga tagttctaat
     2161 ttggagagct gttttatctc caatggctgt gactcagtgc aaggattcca ctcagaacct
     2221 ctttctaaag tttttgcctg atgtgtcttc actggatttt ggggttccca gcaccccatg
     2281 tagatttggg ggtttctttt tgtctccccc actattcaag gactctcctc tttaccaaga
     2341 agcacagaat tctgctgggc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2401 aaaaa
//
