LOCUS       NM_009700               5156 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Mus musculus aquaporin 4 (Aqp4), transcript variant 1, mRNA.
ACCESSION   NM_009700
VERSION     NM_009700.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ide,H., Miike,K., Ohmori,T., Maruyama,K., Izumi,Y., Tanigawa,S. and
            Nishinakamura,R.
  TITLE     Mouse embryonic kidney transplantation identifies maturation
            defects in the medulla
  JOURNAL   Sci Rep 14 (1), 30293 (2024)
   PUBMED   39639083
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Xing,X. and Zhang,S.
  TITLE     Neuroprotective Role of AQP4 Knockdown in Astrocytes After
            Oxygen-Glucose Deprivation
  JOURNAL   Brain Behav 14 (10), e70107 (2024)
   PUBMED   39444081
  REMARK    GeneRIF: Neuroprotective Role of AQP4 Knockdown in Astrocytes After
            Oxygen-Glucose Deprivation.
REFERENCE   3  (bases 1 to 5156)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   4  (bases 1 to 5156)
  AUTHORS   Badaut,J., Ashwal,S., Adami,A., Tone,B., Recker,R., Spagnoli,D.,
            Ternon,B. and Obenaus,A.
  TITLE     Brain water mobility decreases after astrocytic aquaporin-4
            inhibition using RNA interference
  JOURNAL   J Cereb Blood Flow Metab 31 (3), 819-831 (2011)
   PUBMED   20877385
  REMARK    GeneRIF: results demonstrate that apparent diffusion coefficient
            values in normal brain are modulated by astrocytic AQP4; suggest
            imaging changes seen in acute neurologic disorders such as stroke
            and trauma are in part due to changes in tissue AQP4 levels
REFERENCE   5  (bases 1 to 5156)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   6  (bases 1 to 5156)
  AUTHORS   Zelenin,S., Gunnarson,E., Alikina,T., Bondar,A. and Aperia,A.
  TITLE     Identification of a new form of AQP4 mRNA that is developmentally
            expressed in mouse brain
  JOURNAL   Pediatr Res 48 (3), 335-339 (2000)
   PUBMED   10960499
REFERENCE   7  (bases 1 to 5156)
  AUTHORS   Ishida,N., Hirai,S.I. and Mita,S.
  TITLE     Immunolocalization of aquaporin homologs in mouse lacrimal glands
  JOURNAL   Biochem Biophys Res Commun 238 (3), 891-895 (1997)
   PUBMED   9325187
REFERENCE   8  (bases 1 to 5156)
  AUTHORS   Ma,T., Yang,B., Gillespie,A., Carlson,E.J., Epstein,C.J. and
            Verkman,A.S.
  TITLE     Generation and phenotype of a transgenic knockout mouse lacking the
            mercurial-insensitive water channel aquaporin-4
  JOURNAL   J Clin Invest 100 (5), 957-962 (1997)
   PUBMED   9276712
REFERENCE   9  (bases 1 to 5156)
  AUTHORS   Turtzo,L.C., Lee,M.D., Lu,M., Smith,B.L., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A. and Agre,P.
  TITLE     Cloning and chromosomal localization of mouse aquaporin 4:
            exclusion of a candidate mutant phenotype, ataxia
  JOURNAL   Genomics 41 (2), 267-270 (1997)
   PUBMED   9143504
REFERENCE   10 (bases 1 to 5156)
  AUTHORS   Ma,T., Yang,B. and Verkman,A.S.
  TITLE     Gene structure, cDNA cloning, and expression of a mouse
            mercurial-insensitive water channel
  JOURNAL   Genomics 33 (3), 382-388 (1996)
   PUBMED   8660998
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF469168.1, U88623.1,
            AK045357.1, BX633380.1, AK079614.1, BB750519.1 and AC133525.2.
            
            On Dec 7, 2015 this sequence version replaced NM_009700.2.
            
            Summary: This gene encodes a member of the aquaporin family of
            intrinsic membrane proteins that function as water-selective
            channels in the plasma membranes of many cells. This protein is the
            predominant aquaporin found in brain and has an important role in
            brain water homeostasis. Alternatively spliced transcript variants
            encoding different isoforms have been described for this gene. A
            recent study provided evidence for translational readthrough in
            this gene and expression of an additional C-terminally extended
            isoform via the use of an alternative in-frame translation
            termination codon. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1, also known as AQP4.M1 or
            mMIWC2) represents the predominant transcript and encodes two
            isoforms, which result from the use of alternative in-frame
            translation termination codons. The shorter isoform (M1) results
            from translation termination at the upstream UGA stop codon, while
            the longer isoform (M1x) results from UGA stop codon readthrough to
            the downstream UAA termination codon. This RefSeq represents the
            shorter isoform (M1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF469168.1, SRR11927938.3735963.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-261               AF469168.1         1-261
            262-1174            U88623.1           189-1101
            1175-2024           AK045357.1         752-1601
            2025-2025           BX633380.1         202-202             c
            2026-3897           AK045357.1         1602-3473
            3898-5054           AK079614.1         167-1323
            5055-5150           BB750519.1         317-412
            5151-5156           AC133525.2         180919-180924       c
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 8.74 cM"
     gene            1..5156
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /note="aquaporin 4"
                     /db_xref="GeneID:11829"
                     /db_xref="MGI:MGI:107387"
     exon            1..159
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1099
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /note="isoform M1 is encoded by transcript variant 1;
                     mercurial-insensitive water channel"
                     /codon_start=1
                     /product="aquaporin-4 isoform M1"
                     /protein_id="NP_033830.2"
                     /db_xref="CCDS:CCDS29073.1"
                     /db_xref="GeneID:11829"
                     /db_xref="MGI:MGI:107387"
                     /translation="MSDRAAARRWGKCGHSCSRESIMVAFKGVWTQAFWKAVSAEFLA
                     TLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTV
                     AMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGL
                     LVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSF
                     GPAVIMGNWANHWIYWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGS
                     YMEVEDNRSQVETEDLILKPGVVHVIDIDRGEEKKGKDSSGEVLSSV"
     exon            160..574
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /inference="alignment:Splign:2.1.0"
     exon            575..739
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /inference="alignment:Splign:2.1.0"
     exon            740..820
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /inference="alignment:Splign:2.1.0"
     exon            821..5156
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5036..5041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /note="hexamer: ATTAAA"
     polyA_site      5054
                     /gene="Aqp4"
                     /gene_synonym="WCH4"
                     /note="major polyA site"
ORIGIN      
        1 gccacatggt gcagaatctt tccaccccta ctctccaaaa acccaatcag acaagtgccc
       61 gtaatctgac tcccagtgta ctggagcccg ggggcaggca ctgagctgca ctctggccag
      121 ggaaggcatg agtgacagag ctgcggcaag gcggtggggt aagtgtggac attcctgcag
      181 tagagagagc atcatggtgg ctttcaaagg agtctggact caggctttct ggaaggcagt
      241 ctcagcagaa tttctggcca cgcttatctt tgttttgctc ggtgtgggat ccaccataaa
      301 ctggggtggc tcagaaaacc ccttacctgt ggacatggtc ctcatctccc tttgctttgg
      361 actcagcatt gctaccatgg tgcagtgctt tggccacatc agtggtggcc acatcaatcc
      421 cgctgtgact gtagccatgg tgtgcacacg aaagatcagc atcgctaagt ccgtcttcta
      481 catcattgca cagtgcctgg gggccatcat tggagccggc atcctctacc tggtcacacc
      541 tcccagtgtg gttggaggat tgggagtcac cacggttcat ggaaacctca ccgctggcca
      601 tgggctcctg gtggagttaa taatcacttt ccagttggtg ttcactattt ttgccagctg
      661 tgattccaaa cgaactgatg ttactggttc aatagcttta gcaattggat tttccgttgc
      721 aattggacat ttgtttgcaa tcaattatac tggagccagc atgaatccag ctcgatcttt
      781 tggacccgca gttatcatgg gaaactgggc aaaccactgg atatattggg ttggaccaat
      841 catgggcgct gtgctggcag gtgcccttta tgagtatgtc ttctgtcctg atgtggagct
      901 caaacgtcgc cttaaggaag ccttcagcaa agccgcgcag cagacaaaag ggagctacat
      961 ggaggtggag gacaaccgga gccaagtgga gacggaagac ttgatcctga agcccggagt
     1021 ggtgcatgtg attgacattg accgtggaga agagaagaag gggaaagact cttcgggaga
     1081 ggtattgtct tccgtatgac tagaggacag cactgaaggc agaagagact ccctagacct
     1141 ggcctcagat ttcctgccac ccattaagga aacagatttg ttataaatta gacacttgcg
     1201 ggtttcttgc ttcacacctt gttacacagt ttaaataaca catattttac tattatcaat
     1261 ggggggggtg agaaaaagcc tataatggat ataaaatttt aaaacagaaa tctttttgaa
     1321 tgtatcccca aagcaactat gcaactagtg tattggcttc cctttcatta ataactgaaa
     1381 ttatgaacca agatctggtc aagttttgcc gtgcagagca tatggacacc tctgtgagga
     1441 agctggcatt gtccatcgtc ttgactattg acttcattgg attgatttaa aaatgacaaa
     1501 atgcagtatg tcacagaatc atgtgcattc aggagaagac atgcagtaac ttcttccacg
     1561 agctattcct tatttataaa ctacctcgga gggggaaaac attagcaagg gccattgcta
     1621 atacatcatt tgtatttata tatctgactg tcagaaccta actctaattc actataatcc
     1681 tctccccctc agaatccaag aacccatagg gctaaaagta aaaaaaaaat aggaaaaaat
     1741 attaatttct gtccatgtgt tagttccatc aaatgaaagc tatacatggg acacccattg
     1801 aggttcagga cagccagact tgggagtctg gtgggaggtg gaacaaggtg tgctactgcc
     1861 ttgcatccat cacacggaga gggaaacctc tgttatcaaa tgattggtct gttttcagtg
     1921 cacactctta aatgtaccac aaaccattaa ccacctaagc ttttaactat ttcactgact
     1981 ttttaaagct tatttgcaaa actggggaat ttgtttgcca ttttgatccc taaatactag
     2041 cagagacatt ttagaccaga acttgactag tcgagtcctg acttttagtt ggtgtctaaa
     2101 tcctgcttac tctagctggt ataaaccagt cctgccccat cttagctgct gatgctgttt
     2161 tgattcccac gatatgcatc gacaatcggc attgtgagtg taattatacc catttgtgtg
     2221 aattaaaata tatgcagaca aggtgcaacg tggttgccaa ataaaggggt gagcacacag
     2281 cctctgtgca aagctcctag ttaccccatc tgcacatagc ttaccatcat gcttttaagg
     2341 tatttacctc ctgcttggtt tatttgttaa aaccatttat ttctcccata ctgctttgcc
     2401 ttccgcccat cgaatgctct gtggaaaccc cagtttctat gatcataaca gtctagcata
     2461 gcatttatct tagacaatgt gctgcaaatg tcgatttcag taaacaaaag ttagtagtgg
     2521 aagagagaag agccatttct tcaaggacta gcttgtaaat agctggtaat ggagggcttt
     2581 cttctcccaa gcacaattcc aactgtgtgt gacttcttat tgttaatggc agttttgtgt
     2641 ctgtggcagc gagataatgg accactatta aacctgattc tcttcggtgc taggaaactg
     2701 atgtgtgagt tcccagaaag gcacgagggc agcatatgcc tctcggtgcc atggtcatgt
     2761 tctgaggcag atgctgggag catgggccac atttcagggc tttgaatata ctacaccaga
     2821 gacataagct tcctctctga gcagatcacg cagaaccagg gcatagacct cccaggggag
     2881 gtttctgccg attctttcaa atgttttcac aatataccac gcagacttaa gatcagagcg
     2941 cttcagttcg gaaggtgata tcaaccatca acaaatatgt cgggtcaaaa ataaatttac
     3001 ttaacaagga atagttaagc acatttctga ttactctttc aaaatcctgg cctgtgatta
     3061 tttttattat gctagacagc ttgcttcgtt tgtgctcact ttaacagtca tttccagtga
     3121 cagcattcag tatggtacaa actgaaaaac agggaatcat agcttagtga tttgtttgct
     3181 agcagctggc agagtgccag gcacacagta ggcaatcaaa acaagcatgt tgtttgaata
     3241 tatatacata tatacatata tacatatata catatataca tatatgtgta tatatgattc
     3301 ttatttggga tttaaaatac caataatctc ctctaatttt taattttgcc caatatttag
     3361 tgaaaacata atttttagtg tcatcaataa aagcaacatg gacttggaaa caaagagcat
     3421 atttttacat tctactgttt gaaggcagag agtgactact tcacactctt cgtctttgca
     3481 atatgtcttg catttcactc acggctctgc cagtaaaaaa tgtaatgaaa ttgtcccttt
     3541 ctaatgacat cgatgcagca ggggtctatt gccttgtgga tgacaccaaa ttatacaaca
     3601 tattaggaga tcggggattt tccctttaat cccttcttat taatgaagtg catagtgccg
     3661 ttcccaagag acagctactg acagatacac cgcacagaga tcagagagga aaaatcagga
     3721 agacataaaa gatttatacg agccatgaaa caatgccaac tgtctgttcc ctcaggagga
     3781 gaacacagac acaccacaaa tttcaaggtg gtccatacac tggaaatgta catacatagt
     3841 ctgtcaaagg agaacagaag gaaatctttt ttttttaatt ttgagcattt tttttttcaa
     3901 atcagagact ctgattttta aatgtgtgtt tccattttct tccaaatgtt ctaccctttt
     3961 atactacaag aaactgtttc ctgtagccta agtcactggt aattttacaa cttgttcctg
     4021 tgatttgcca ctatcatgag tcctcttccg ttcgatcttc agaggggtgt cccaccacag
     4081 ctcggacact gctggccaca gctgctccct gatgaagatt tcataaggag ttggtagtct
     4141 ctgggaaatg ggctgatata ttagaggtca atttcaaaaa ctgcaacatt tctcctagga
     4201 gaatccaggc aatgtgtgca ctgctctaac cccactgaga accctgacat ctcaaaatga
     4261 agggactaat taaaatggtg gcattggtcc ttgcctggcc tttgttgtgt gatgttgaca
     4321 ccttcctcat agatcagaaa tctcacagag acactgcctc tgtgacaatt gaagccagag
     4381 agccaaacag acaatcttac aaaagccatg agacgctcta atcactaaac tacaggatac
     4441 gtgaacttgg aattgtgcaa gcatggcctc tacttgaaag tggactataa caaaagaaac
     4501 tttatttacc tcaacttttc tgagttcatg tccaggtgtc aatacttttc agcacacctt
     4561 aactaacaca aatatttgaa atccaaaatt ctcagaaaga aatgttaaga cgcttaactt
     4621 caaaaatcaa aaattgcgga tattcacttt tggcacataa cttccctgaa aacacacacc
     4681 aaacaaatat taaacaacac atacagtgaa cactacattt aagagcagtt atattgttac
     4741 tgttttaagt caaaattcaa gagcttaaaa aatgcccaac atatatattc cattgcaatg
     4801 tattcttaga ttgttttcag gcttgatcaa ataaacatgg aatttgtgga aatatatttt
     4861 aaaaatctat ttatcatttt ctctcccaat tcacaattaa cttgcgactt atgaggaact
     4921 aaaaaaataa aatgaatgcc tggttatatt cacatttatt aactaaatat tattccaact
     4981 ttagagttaa tgttaaatgg atttgaactg taatggctaa tatttggaaa aatctattaa
     5041 aagtattagc agtgtggctg ggttctgttt cttgtgttat atgtcatact attagtatca
     5101 tacaattaag tcttcaaatg ttttaaaaat aaaccatata tttgatggtg tttaag
//
