LOCUS       NM_145220               2958 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus adaptor protein, phosphotyrosine interaction, PH
            domain and leucine zipper containing 2 (Appl2), mRNA.
ACCESSION   NM_145220
VERSION     NM_145220.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Ryu,J., Hadley,J.T., Li,Z., Dong,F., Xu,H., Xin,X., Zhang,Y.,
            Chen,C., Li,S., Guo,X., Zhao,J.L., Leach,R.J., Abdul-Ghani,M.A.,
            DeFronzo,R.A., Kamat,A., Liu,F. and Dong,L.Q.
  TITLE     Adiponectin Alleviates Diet-Induced Inflammation in the Liver by
            Suppressing MCP-1 Expression and Macrophage Infiltration
  JOURNAL   Diabetes 70 (6), 1303-1316 (2021)
   PUBMED   34162682
  REMARK    GeneRIF: Adiponectin Alleviates Diet-Induced Inflammation in the
            Liver by Suppressing MCP-1 Expression and Macrophage Infiltration.
REFERENCE   3  (bases 1 to 2958)
  AUTHORS   Wang,B., Lin,H., Li,X., Lu,W., Kim,J.B., Xu,A. and Cheng,K.K.Y.
  TITLE     The adaptor protein APPL2 controls glucose-stimulated insulin
            secretion via F-actin remodeling in pancreatic beta-cells
  JOURNAL   Proc Natl Acad Sci U S A 117 (45), 28307-28315 (2020)
   PUBMED   33122440
  REMARK    GeneRIF: The adaptor protein APPL2 controls glucose-stimulated
            insulin secretion via F-actin remodeling in pancreatic beta-cells.
REFERENCE   4  (bases 1 to 2958)
  AUTHORS   Gao,C., Yan,T., Chen,X., Cheng,K.K.Y., Xu,A. and Shen,J.
  TITLE     APPL2 Negatively Regulates Olfactory Functions by Switching Fate
            Commitments of Neural Stem Cells in Adult Olfactory Bulb via
            Interaction with Notch1 Signaling
  JOURNAL   Neurosci Bull 36 (9), 997-1008 (2020)
   PUBMED   32468397
  REMARK    GeneRIF: APPL2 Negatively Regulates Olfactory Functions by
            Switching Fate Commitments of Neural Stem Cells in Adult Olfactory
            Bulb via Interaction with Notch1 Signaling.
REFERENCE   5  (bases 1 to 2958)
  AUTHORS   Pereira,M., Ko,J.H., Logan,J., Protheroe,H., Kim,K.B., Tan,A.L.M.,
            Croucher,P.I., Park,K.S., Rotival,M., Petretto,E., Bassett,J.D.,
            Williams,G.R. and Behmoaras,J.
  TITLE     A trans-eQTL network regulates osteoclast multinucleation and bone
            mass
  JOURNAL   Elife 9, e55549 (2020)
   PUBMED   32553114
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2958)
  AUTHORS   Cheng,K.K., Zhu,W., Chen,B., Wang,Y., Wu,D., Sweeney,G., Wang,B.,
            Lam,K.S. and Xu,A.
  TITLE     The adaptor protein APPL2 inhibits insulin-stimulated glucose
            uptake by interacting with TBC1D1 in skeletal muscle
  JOURNAL   Diabetes 63 (11), 3748-3758 (2014)
   PUBMED   24879834
REFERENCE   7  (bases 1 to 2958)
  AUTHORS   Tan,Y., You,H., Coffey,F.J., Wiest,D.L. and Testa,J.R.
  TITLE     Appl1 is dispensable for Akt signaling in vivo and mouse T-cell
            development
  JOURNAL   Genesis 48 (9), 531-539 (2010)
   PUBMED   20665729
REFERENCE   8  (bases 1 to 2958)
  AUTHORS   Tan,Y., You,H., Wu,C., Altomare,D.A. and Testa,J.R.
  TITLE     Appl1 is dispensable for mouse development, and loss of Appl1 has
            growth factor-selective effects on Akt signaling in murine
            embryonic fibroblasts
  JOURNAL   J Biol Chem 285 (9), 6377-6389 (2010)
   PUBMED   20040596
REFERENCE   9  (bases 1 to 2958)
  AUTHORS   Wang,C., Xin,X., Xiang,R., Ramos,F.J., Liu,M., Lee,H.J., Chen,H.,
            Mao,X., Kikani,C.K., Liu,F. and Dong,L.Q.
  TITLE     Yin-Yang regulation of adiponectin signaling by APPL isoforms in
            muscle cells
  JOURNAL   J Biol Chem 284 (46), 31608-31615 (2009)
   PUBMED   19661063
REFERENCE   10 (bases 1 to 2958)
  AUTHORS   Mao,X., Kikani,C.K., Riojas,R.A., Langlais,P., Wang,L., Ramos,F.J.,
            Fang,Q., Christ-Roberts,C.Y., Hong,J.Y., Kim,R.Y., Liu,F. and
            Dong,L.Q.
  TITLE     APPL1 binds to adiponectin receptors and mediates adiponectin
            signalling and function
  JOURNAL   Nat Cell Biol 8 (5), 516-523 (2006)
   PUBMED   16622416
  REMARK    Erratum:[Nat Cell Biol. 2006 Jun;8(6):642]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY113706.1.
            
            On Apr 5, 2007 this sequence version replaced NM_145220.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY113706.1, AK160075.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2958              AY113706.1         3-2960
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 41.29 cM"
     gene            1..2958
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /note="adaptor protein, phosphotyrosine interaction, PH
                     domain and leucine zipper containing 2"
                     /db_xref="GeneID:216190"
                     /db_xref="MGI:MGI:2384914"
     exon            1..137
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..2072
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /note="adapter protein containing PH domain, PTB domain
                     and leucine zipper motif 2; dip13-beta"
                     /codon_start=1
                     /product="DCC-interacting protein 13-beta"
                     /protein_id="NP_660255.1"
                     /db_xref="CCDS:CCDS24078.1"
                     /db_xref="GeneID:216190"
                     /db_xref="MGI:MGI:2384914"
                     /translation="MPAVDKLLLEEALQDSPQARSLLSVFEEDAGTLTDYTNQLLQAM
                     QRVYGAQNEMCLATQQLSRQLLAYEKQNFALGKGDEEVISTLHYFSKVMDELNGLHTE
                     LAKQLADTMVLPVIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKAK
                     TEIVKEVAAARRKQHLSSLQYYCALNALQYRKRAAMMEPLIGFAHGQINFFKRGAEMF
                     SKSMDGFLSSVKDMVQSIQVELEAEADKMRVSQQELLSVSESVYTPDIDVATAQINRN
                     LIQKTGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVD
                     CEDRRYCFQISTPSGKPGIILQAESRKEYEEWICAVNNISRQIYLTDNPEAVAIKLNQ
                     TALQAVTPITSFGKKQESSCSSQNIKNSDIEDDNIVPKATASIPETEELIAPGTPIQF
                     DIVLPATEFLDQNRGGRRTNPFGETEDGSFPEAEDSLLQQMFIVRFLGSMAVKTDSTA
                     EVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVSRACFELTSVTQFAA
                     HQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALA
                     RLMLSVPLTNDGKYVLLNDQADDTGGSPSENRGAESEA"
     exon            138..236
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            237..296
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            297..368
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            369..456
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            457..498
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            499..557
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            558..704
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            705..787
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            788..946
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1135
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1178
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1235
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1324
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1483
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1536
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1711
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1748
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1889
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1937
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2946
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2924..2929
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /note="hexamer: AATAAA"
     polyA_site      2946
                     /gene="Appl2"
                     /gene_synonym="DIP13 beta; Dip3 beta; Dip3b"
                     /note="major polyA site"
ORIGIN      
        1 aggcgcgggc tccgagctaa gtctcaagtc cccgagcgcc gagtgtcccc tcctcgcatc
       61 ccgcgctccc tcgccgccgc gccatgcccg ccgtggacaa gctcctgctg gaggaggcgc
      121 tgcaggacag cccccaggcg cgctccttgc tgagtgtatt tgaagaagat gctggcaccc
      181 tcacagatta caccaaccag ctgctgcagg ccatgcagcg tgtctatggc gcacagaatg
      241 aaatgtgcct ggccactcag cagctttcca ggcagctgct ggcatatgaa aaacagaatt
      301 ttgcactggg caaaggcgat gaagaagtaa tttcaacact acattatttt tccaaagtga
      361 tggatgagct caatggtctc cacacagagc tggccaagca actggcagac actatggttc
      421 tccctgttat acagtttcga gagaaggatc tcacagaagt cagcactttg aaggatctct
      481 ttggacttgc cagcagcgag cacgacctgt caatggcaaa gtatagcagg ctccctaaga
      541 agaaggagaa cgagaaggcg aagacggaaa ttgtgaaaga ggtggcggct gcccggcgga
      601 aacagcacct ctcatccctt cagtactact gcgcactcaa cgcactgcag taccgcaagc
      661 gggcggccat gatggaaccc ttgataggct ttgcccacgg acagatcaac tttttcaaga
      721 gaggagcaga gatgttctcc aaaagtatgg acggcttctt gtcatccgtt aaagacatgg
      781 ttcagagcat tcaggtggaa ctggaagctg aggcagacaa gatgcgggtg tcccagcaag
      841 agttgctttc ggtcagtgaa tctgtttaca ctccggacat tgatgtggcc acagcgcaga
      901 tcaacaggaa cctcatccag aagactggtt acctcaatct cagaaacaag accggactgg
      961 tcaccaccac gtgggagagg ctgtacttct tcacccaagg cgggaacctc atgtgccagc
     1021 cccggggcgc tgtggctgga ggcttgattc aggacctgga caactgctct gtgatggccg
     1081 tggattgtga agaccgacga tactgcttcc agatctccac gcccagtggc aaaccgggaa
     1141 taattctcca agcagagagc aggaaggaat atgaagagtg gatatgtgca gtaaacaaca
     1201 tctccaggca gatctacctg acggacaacc cagaggcagt agccatcaag ttgaatcaga
     1261 cggctcttca ggcggtgacg ccaattacaa gctttgggaa gaaacaagaa agctcctgct
     1321 ccagtcaaaa cataaaaaat tcagacatag aagatgacaa tattgttccc aaagcaacag
     1381 ccagcattcc tgaaacagag gaactgattg cacctgggac acccattcag tttgatattg
     1441 tacttccggc aacagaattc cttgaccaga acaggggtgg caggcgcacc aaccctttcg
     1501 gtgagacgga ggatggctca ttccctgaag cagaagactc tcttctgcag cagatgttca
     1561 tcgttcggtt tttgggatca atggcagtta agacagacag cacggctgaa gtgatttatg
     1621 aggcaatgag acaagtactg gctgctcgag ctattcacaa catcttccgc atgaccgaat
     1681 cccatctcat ggtcaccagt caaactctga ggttgataga tcctcagact caagtgtcaa
     1741 gggcctgttt tgagcttacc agtgtcacac agtttgctgc tcatcaagaa aacaaaagac
     1801 tggttggctt tgtcatccgg gtgccggagt ccacaggaga agagtctctg agcacataca
     1861 tttttgaaag caactcagaa ggagaaaaga tatgctatgc tattaatttg gggaaagaaa
     1921 ttattgaggt tcaaaaggat ccagaagcac tggctcgatt aatgctgtct gtaccactaa
     1981 ccaatgatgg aaagtatgta ctgttaaacg atcaagcaga tgacactggc ggaagtccaa
     2041 gtgaaaacag aggcgcagaa tctgaagcat aactcatttg ggcatcgggg gtcagagcac
     2101 gtgggaagga gagccactcc ttaagcagtt ttcaacttct ctgccgtaca ggcacagtga
     2161 cctgaattta gaacaccgac aatcacgtgc ggagtgtacc cttggatgcc ctggcacaga
     2221 aacttgggag ggagacaata agaaatgggt gagagcgtgc ctacactcta caaatgctat
     2281 tttaggtgct ttgtggtaag tctttgcttc agactgttgg gtacccagaa cgagagctat
     2341 aaaaatgcat tgttcacttt atttggatag cagcgcttca gttcctagga tccttttata
     2401 aatggtaaaa ccctggattt acaatttgtt aaatactaaa tagttcctat taatatgatc
     2461 tatttttgta ttttacttag ttttaagtgc ctgtcattct gaagattata tatttataac
     2521 ccaatggtct catgcctgga cctcatggca ctgctatgca atttggtctc aaacagacag
     2581 caaagccttt acaacagaac ctttctgaac cagctttcat tcaaaagctt caggtcctct
     2641 tagatgcttt ctctggctac tcactgtgag caagggacag ttctactagt tcctgtttct
     2701 tctgagccag accttcctca aagggaccaa ttaatttaaa actcacttga agctcagctg
     2761 gctgtggggc tgtctttgaa gttctggttt ctaccttgat gtttctggtt cgtggggtcc
     2821 tggaccaatc tccaacccct aattatgaag taaaggtctt gaaatcatgc agcataaatt
     2881 aattgtaacc atcttcaaca acctgtcttt gtaataattt agcaataaaa actgtttcat
     2941 ctcaaaaaaa aaaaaaaa
//
