LOCUS       NM_009696               1288 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus apolipoprotein E (Apoe), transcript variant 1, mRNA.
ACCESSION   NM_009696
VERSION     NM_009696.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Tran,K.M., Kwang,N.E., Butler,C.A., Gomez-Arboledas,A.,
            Kawauchi,S., Mar,C., Chao,D., Barahona,R.A., Da Cunha,C.,
            Tsourmas,K.I., Shi,Z., Wang,S., Collins,S., Walker,A., Shi,K.X.,
            Alcantara,J.A., Neumann,J., Duong,D.M., Seyfried,N.T., Tenner,A.J.,
            LaFerla,F.M., Hohsfield,L.A., Swarup,V., MacGregor,G.R. and
            Green,K.N.
  TITLE     APOE Christchurch enhances a disease-associated microglial response
            to plaque but suppresses response to tau pathology
  JOURNAL   Mol Neurodegener 20 (1), 9 (2025)
   PUBMED   39844286
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Brandao,W., Jain,N., Yin,Z., Kleemann,K.L., Carpenter,M., Bao,X.,
            Serrano,J.R., Tycksen,E., Durao,A., Barry,J.L., Baufeld,C.,
            Guneykaya,D., Zhang,X., Litvinchuk,A., Jiang,H., Rosenzweig,N.,
            Pitts,K.M., Aronchik,M., Yahya,T., Cao,T., Takahashi,M.K.,
            Krishnan,R., Davtyan,H., Ulrich,J.D., Blurton-Jones,M., Ilin,I.,
            Weiner,H.L., Holtzman,D.M. and Butovsky,O.
  TITLE     Inhaled xenon modulates microglia and ameliorates disease in mouse
            models of amyloidosis and tauopathy
  JOURNAL   Sci Transl Med 17 (781), eadk3690 (2025)
   PUBMED   39813317
REFERENCE   3  (bases 1 to 1288)
  AUTHORS   Huynh,T.N., Fikse,E.N., De La Torre,A.L., Havrda,M.C., Chang,C.C.Y.
            and Chang,T.Y.
  TITLE     Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Aging
            Apolipoprotein E4 Mice Alters Their Brains' Inflammatory Profiles
  JOURNAL   Int J Mol Sci 25 (24), 13690 (2024)
   PUBMED   39769453
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1288)
  AUTHORS   Wijesinghe,P., Li,H.R., Ai,Z., Campbell,M., Chen,S.X., Xi,J.,
            Pham,W. and Matsubara,J.A.
  TITLE     Apolipoprotein E dysfunction in Alzheimer's disease: a study on
            miRNA regulation, glial markers, and amyloid pathology
  JOURNAL   Front Aging Neurosci 16, 1495615 (2024)
   PUBMED   39744521
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1288)
  AUTHORS   Alcicek,F.C., Dybas,J., Bulat,K., Mohaissen,T.,
            Szczesny-Malysiak,E., Franczyk-Zarow,M. and Marzec,K.M.
  TITLE     Hypoxia induces robust ATP release from erythrocytes in ApoE-LDLR
            double-deficient mice
  JOURNAL   Front Physiol 15, 1497346 (2024)
   PUBMED   39678689
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1288)
  AUTHORS   Holtzman,D.M., Herz,J. and Bu,G.
  TITLE     Apolipoprotein E and apolipoprotein E receptors: normal biology and
            roles in Alzheimer disease
  JOURNAL   Cold Spring Harb Perspect Med 2 (3), a006312 (2012)
   PUBMED   22393530
  REMARK    Review article
REFERENCE   7  (bases 1 to 1288)
  AUTHORS   Herz,J., Clouthier,D.E. and Hammer,R.E.
  TITLE     LDL receptor-related protein internalizes and degrades uPA-PAI-1
            complexes and is essential for embryo implantation
  JOURNAL   Cell 71 (3), 411-421 (1992)
   PUBMED   1423604
  REMARK    Erratum:[Cell. 1993 May 7;73(3):428. doi:
            10.1016/0092-8674(93)90130-i. PMID: 8490961]
REFERENCE   8  (bases 1 to 1288)
  AUTHORS   Plump,A.S., Smith,J.D., Hayek,T., Aalto-Setala,K., Walsh,A.,
            Verstuyft,J.G., Rubin,E.M. and Breslow,J.L.
  TITLE     Severe hypercholesterolemia and atherosclerosis in apolipoprotein
            E-deficient mice created by homologous recombination in ES cells
  JOURNAL   Cell 71 (2), 343-353 (1992)
   PUBMED   1423598
REFERENCE   9  (bases 1 to 1288)
  AUTHORS   Zhang,S.H., Reddick,R.L., Piedrahita,J.A. and Maeda,N.
  TITLE     Spontaneous hypercholesterolemia and arterial lesions in mice
            lacking apolipoprotein E
  JOURNAL   Science 258 (5081), 468-471 (1992)
   PUBMED   1411543
REFERENCE   10 (bases 1 to 1288)
  AUTHORS   Piedrahita,J.A., Zhang,S.H., Hagaman,J.R., Oliver,P.M. and Maeda,N.
  TITLE     Generation of mice carrying a mutant apolipoprotein E gene
            inactivated by gene targeting in embryonic stem cells
  JOURNAL   Proc Natl Acad Sci U S A 89 (10), 4471-4475 (1992)
   PUBMED   1584779
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY313639.1, BY772289.1,
            AK134921.1 and AI836733.1.
            
            On Apr 3, 2015 this sequence version replaced NM_009696.3.
            
            Summary: This gene encodes a member of the apolipoprotein A1/A4/E
            family of proteins. This protein is involved in the transport of
            lipoproteins in the blood. It binds to a specific liver and
            peripheral cell receptor, and is essential for the normal
            catabolism of triglyceride-rich lipoprotein constituents.
            Homozygous knockout mice for this gene accumulate high levels of
            cholesterol in the blood and develop atherosclerosis. Different
            alleles of this gene have been associated with either increased
            risk or a protective effect for Alzheimer's disease in human
            patients. This gene maps to chromosome 7 in a cluster with the
            related apolipoprotein C1, C2 and C4 genes. [provided by RefSeq,
            Apr 2015].
            
            Transcript Variant: This variant (1) differs in the 5' UTR compared
            to variant 3. Variants 1-4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY313639.1         3-24
            23-136              BY772289.1         2-115
            137-1244            AK134921.1         2-1109
            1245-1288           AI836733.1         1-44                c
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.94 cM"
     gene            1..1288
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /note="apolipoprotein E"
                     /db_xref="GeneID:11816"
                     /db_xref="MGI:MGI:88057"
     exon            1..178
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /inference="alignment:Splign:2.1.0"
     exon            179..244
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1137
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /codon_start=1
                     /product="apolipoprotein E precursor"
                     /protein_id="NP_033826.2"
                     /db_xref="CCDS:CCDS20912.1"
                     /db_xref="GeneID:11816"
                     /db_xref="MGI:MGI:88057"
                     /translation="MKALWAVLLVTLLTGCLAEGEPEVTDQLEWQSNQPWEQALNRFW
                     DYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRAR
                     LGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMR
                     DAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQ
                     AFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFE
                     PIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ"
     exon            245..413
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /inference="alignment:Splign:2.1.0"
     exon            414..1273
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1227..1232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /note="hexamer: AATAAA"
     polyA_site      1247
                     /gene="Apoe"
                     /gene_synonym="Apo-E"
                     /note="major polyA site"
ORIGIN      
        1 tttcctctgc cctgctgtga agggggagag aacaacccgc ctcgtgacag ggggctggca
       61 cagcccgccc tagccctgag gagggggcgg gacaggggga gtcctataat tggaccggtc
      121 tgggatccga tcccctgctc agaccctgga ggctaaggac ttgtttcgga aggagctgac
      181 tggccaatca caattgcgaa gatgaaggct ctgtgggccg tgctgttggt cacattgctg
      241 acaggatgcc tagccgaggg agagccggag gtgacagatc agctcgagtg gcaaagcaac
      301 caaccctggg agcaggccct gaaccgcttc tgggattacc tgcgctgggt gcagacgctg
      361 tctgaccagg tccaggaaga gctgcagagc tcccaagtca cacaagaact gacggcactg
      421 atggaggaca ctatgacgga agtaaaggct tacaaaaagg agctggagga acagctgggt
      481 ccagtggcgg aggagacacg ggccaggctg ggcaaagagg tgcaggcggc acaggcccga
      541 ctcggagccg acatggagga tctacgcaac cgactcgggc agtaccgcaa cgaggtgcac
      601 accatgctgg gccagagcac agaggagata cgggcgcggc tctccacaca cctgcgcaag
      661 atgcgcaagc gcttgatgcg ggatgccgag gatctgcaga agcgcctagc tgtgtacaag
      721 gcaggggcac gcgagggcgc cgagcgcggt gtgagtgcca tccgtgagcg cctggggcct
      781 ctggtggagc aaggtcgcca gcgcactgcc aacctaggcg ctggggccgc ccagcctctg
      841 cgcgatcgcg cccaggcttt tggtgaccgc atccgagggc ggctggagga agtgggcaac
      901 caggcccgtg accgcctaga ggaggtgcgt gagcacatgg aggaggtgcg ctccaagatg
      961 gaggaacaga cccagcaaat acgcctgcag gcggagatct tccaggcccg cctcaagggc
     1021 tggttcgagc caatagtgga agacatgcat cgccagtggg caaacctgat ggagaagata
     1081 caggcctctg tggctaccaa ccccatcatc accccagtgg cccaggagaa tcaatgagta
     1141 tccttctcct gtcctgcaac aacatccata tccagccagg tggccctgtc tcaagcacct
     1201 ctctggccct ctggtggccc ttgcttaata aagattctcc gagcacattc tgagtctctg
     1261 tgagtgattc caaaaaaaaa aaaaaaaa
//
