LOCUS       NM_001402727           12587 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus APC, WNT signaling pathway regulator (Apc), transcript
            variant 4, mRNA.
ACCESSION   NM_001402727 XM_006525530
VERSION     NM_001402727.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Fimiani,C., Pereira,J.A., Gerber,J., Berg,I., DeGeer,J.,
            Bachofner,S., Fischer,J.S., Kauffmann,M. and Suter,U.
  TITLE     The E3 ubiquitin ligase Nedd4 fosters developmental myelination in
            the mouse central and peripheral nervous system
  JOURNAL   Glia 73 (2), 422-444 (2025)
   PUBMED   39511974
REFERENCE   2  (bases 1 to 12587)
  AUTHORS   Xu,X., Fang,M., Chen,L., Huang,H., Dai,Z.M., Yang,J. and Qiu,M.
  TITLE     Nzf2 promotes oligodendrocyte differentiation and regeneration via
            repressing HDAC1-mediated histone deacetylation
  JOURNAL   Sci Adv 10 (50), eadf8405 (2024)
   PUBMED   39671488
REFERENCE   3  (bases 1 to 12587)
  AUTHORS   Georgieva,T.G., Darmoul,D., Chen,H., Cui,H., Rice,P.F.S.,
            Barton,J.K., Besselsen,D.G. and Ignatenko,N.A.
  TITLE     Kallikrein-Related Peptidase 6 Contributes to Murine Intestinal
            Tumorigenesis Driven by a Mutant Adenomatous polyposis coli Gene
  JOURNAL   Cancers (Basel) 16 (22), 3842 (2024)
   PUBMED   39594797
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12587)
  AUTHORS   Islam,M., Yang,Y., Simmons,A.J., Shah,V.M., Musale,K.P., Xu,Y.,
            Tasneem,N., Chen,Z., Trinh,L.T., Molina,P., Ramirez-Solano,M.A.,
            Sadien,I.D., Dou,J., Rolong,A., Chen,K., Magnuson,M.A.,
            Rathmell,J.C., Macara,I.G., Winton,D.J., Liu,Q., Zafar,H.,
            Kalhor,R., Church,G.M., Shrubsole,M.J., Coffey,R.J. and Lau,K.S.
  TITLE     Temporal recording of mammalian development and precancer
  JOURNAL   Nature 634 (8036), 1187-1195 (2024)
   PUBMED   39478207
REFERENCE   5  (bases 1 to 12587)
  AUTHORS   Sadien,I.D., Adler,S., Mehmed,S., Bailey,S., Sawle,A.,
            Couturier,D.L., Eldridge,M., Adams,D.J., Kemp,R., Lourenco,F.C. and
            Winton,D.J.
  TITLE     Polyclonality overcomes fitness barriers in Apc-driven
            tumorigenesis
  JOURNAL   Nature 634 (8036), 1196-1203 (2024)
   PUBMED   39478206
  REMARK    GeneRIF: Polyclonality overcomes fitness barriers in Apc-driven
            tumorigenesis.
REFERENCE   6  (bases 1 to 12587)
  AUTHORS   Justice,M.J., Gilbert,D.J., Kinzler,K.W., Vogelstein,B.,
            Buchberg,A.M., Ceci,J.D., Matsuda,Y., Chapman,V.M., Patriotis,C.,
            Makris,A. et al.
  TITLE     A molecular genetic linkage map of mouse chromosome 18 reveals
            extensive linkage conservation with human chromosomes 5 and 18
  JOURNAL   Genomics 13 (4), 1281-1288 (1992)
   PUBMED   1354644
REFERENCE   7  (bases 1 to 12587)
  AUTHORS   Su,L.K., Kinzler,K.W., Vogelstein,B., Preisinger,A.C., Moser,A.R.,
            Luongo,C., Gould,K.A. and Dove,W.F.
  TITLE     Multiple intestinal neoplasia caused by a mutation in the murine
            homolog of the APC gene
  JOURNAL   Science 256 (5057), 668-670 (1992)
   PUBMED   1350108
  REMARK    Erratum:[Science 1992 May 22;256(5060):1114]
REFERENCE   8  (bases 1 to 12587)
  AUTHORS   Moser,A.R., Dove,W.F., Roth,K.A. and Gordon,J.I.
  TITLE     The Min (multiple intestinal neoplasia) mutation: its effect on gut
            epithelial cell differentiation and interaction with a modifier
            system
  JOURNAL   J Cell Biol 116 (6), 1517-1526 (1992)
   PUBMED   1541640
REFERENCE   9  (bases 1 to 12587)
  AUTHORS   Joslyn,G., Carlson,M., Thliveris,A., Albertsen,H., Gelbert,L.,
            Samowitz,W., Groden,J., Stevens,J., Spirio,L., Robertson,M. et al.
  TITLE     Identification of deletion mutations and three new genes at the
            familial polyposis locus
  JOURNAL   Cell 66 (3), 601-613 (1991)
   PUBMED   1678319
REFERENCE   10 (bases 1 to 12587)
  AUTHORS   Wolff,G.L., Greenman,D.L., Frigeri,L.G., Morrissey,R.L., Suber,R.L.
            and Felton,R.P.
  TITLE     Diabetogenic response to streptozotocin varies among obese yellow
            and among lean agouti (BALB/c x VY)F1 hybrid mice
  JOURNAL   Proc Soc Exp Biol Med 193 (2), 155-163 (1990)
   PUBMED   2137249
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114003.4, AL591373.8 and AC090534.10.
            
            On Mar 21, 2022 this sequence version replaced XM_006525530.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.771799.1,
                                           SRR13948564.3072054.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               AC114003.4         186067-186443
            378-530             AL591373.8         55398-55550         c
            531-612             AL591373.8         48176-48257         c
            613-811             AL591373.8         47301-47499         c
            812-920             AL591373.8         44008-44116         c
            921-1034            AL591373.8         39878-39991         c
            1035-1118           AL591373.8         37203-37286         c
            1119-1223           AL591373.8         26613-26717         c
            1224-1322           AL591373.8         20426-20524         c
            1323-1701           AL591373.8         17754-18132         c
            1702-1797           AL591373.8         16494-16589         c
            1798-1851           AL591373.8         15240-15293         c
            1852-1991           AL591373.8         11833-11972         c
            1992-2069           AL591373.8         10912-10989         c
            2070-2186           AL591373.8         10176-10292         c
            2187-2401           AL591373.8         5648-5862           c
            2402-6951           AL591373.8         1-4550              c
            6952-12587          AC090534.10        116981-122616       c
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 18.53 cM"
     gene            1..12587
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="APC, WNT signaling pathway regulator"
                     /db_xref="GeneID:11789"
                     /db_xref="MGI:MGI:88039"
     exon            1..377
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            378..530
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     CDS             396..8978
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="isoform 1 is encoded by transcript variant 4;
                     adenomatous polyposis coli protein; multiple intestinal
                     neoplasia; adenomatosis polyposis coli"
                     /codon_start=1
                     /product="adenomatous polyposis coli protein isoform 1"
                     /protein_id="NP_001389656.1"
                     /db_xref="GeneID:11789"
                     /db_xref="MGI:MGI:88039"
                     /translation="MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNM
                     KEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVS
                     SRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQL
                     QNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIAR
                     IQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQ
                     SPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLA
                     MSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQ
                     PDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCV
                     LMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDH
                     YSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRN
                     LSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICA
                     VDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLL
                     QHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAA
                     ALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPK
                     ASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGS
                     LDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIH
                     TSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEY
                     KRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHM
                     DDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQN
                     TSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLC
                     QEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDI
                     SSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKG
                     TTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQT
                     AEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSP
                     SKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIIS
                     PSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQ
                     RVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGN
                     ETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKL
                     AQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFS
                     TATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVT
                     PDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTK
                     KKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEK
                     LPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKG
                     KESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPD
                     RGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEP
                     SKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLK
                     SESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSL
                     HQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGE
                     KSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTM
                     IHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQ
                     TSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLP
                     RTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQM
                     SNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASF
                     ESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDG
                     RPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASS
                     ESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAAS
                     GAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSI
                     KDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAE
                     TCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVST
                     NTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV"
     exon            531..612
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            613..811
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            812..920
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1034
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1118
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1223
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1322
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1701
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1797
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1851
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..1991
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2069
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2186
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2401
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..12587
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9774..9779
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="hexamer: AATAAA"
     polyA_site      9799
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="major polyA site"
     regulatory      9937..9942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="hexamer: AATAAA"
     polyA_site      9971
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="major polyA site"
     regulatory      12568..12573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="hexamer: ATTAAA"
     polyA_site      12587
                     /gene="Apc"
                     /gene_synonym="CC1; mAPC; Min"
                     /note="major polyA site"
ORIGIN      
        1 gcactgcccg cttcccgcct ccaacaagat ggcgaaggcc ctgtgctgag ggggcggagt
       61 gtggctgccg gaaacccggc cgctaccttg ggggagcttc gggcgaaggc ccgggagcag
      121 cggaccgagg ctggcgcgat gctgttcccg gggagcgcag tcggctaccg ttgaggaagg
      181 tggagtgagg agtggccctt ccagcgcccc ctatgtacgc cttcctgcgc tcggggccgg
      241 tcgccgcgtt gcccgcctcc gtaccgcccg tgactctcgg ggcccggagc tccggcggcg
      301 gccggggtcg agtcccgggg gaggggaggc gcccgggcgg cgcccgagct tgcggccgcg
      361 gagcgagcgt ctggcaggtc caagggtagc caaggatggc tgcagcttca tatgatcagt
      421 tgttaaagca agttgaggca ctgaagatgg agaactcaaa tcttcgacaa gagctagaag
      481 ataattccaa tcatcttaca aaactggaaa ctgaggcatc taatatgaag gaagtactta
      541 agcagctaca gggaagtatt gaagatgaga ctatgacttc tggacagatt gacttactag
      601 agcgtcttaa agaatttaac ttagatagta atttccccgg agtgaaacta cgctcaaaaa
      661 tgtcccttcg ctcctacgga agtcgggaag gatctgtatc cagccgttca ggagaatgca
      721 gtcctgtccc catggggtca ttcccaagaa gaacatttgt aaatggaagc agagagagta
      781 ctgggtatct agaagagctt gaaaaagaaa gatcattact ccttgctgat cttgacaaag
      841 aagagaagga aaaggactgg tattatgctc aacttcagaa cctcacaaaa agaatagata
      901 gcctgccttt aactgaaaat ttttccttac agacagacat gacaagacgg cagctggagt
      961 atgaagcaag gcagatcagg gctgcaatgg aggagcagct tggcacctgc caggacatgg
     1021 agaagcgtgc acagcgaaga atagccagga tccagcaaat agaaaaggac atactgcgcg
     1081 tgcgccagct tttacagtcc caggcggcgg aagcggagag gtcatctcag agcaggcatg
     1141 atgctgcctc ccatgaagct ggccggcagc acgaaggcca cggagtggca gaaagcaaca
     1201 ccgcagcctc cagtagtggt cagagtccag ctacacgtgt ggatcacgaa acagccagtg
     1261 ttttgagttc tagcggcacg cactctgctc ctcgaaggtt gacaagtcat ctggggacaa
     1321 aggtggaaat ggtgtattcc ttgttgtcaa tgcttggtac tcatgataag gacgatatgt
     1381 cacgaacttt gctagctatg tccagctccc aagacagctg tatatccatg cggcagtctg
     1441 gatgtcttcc tctcctcatc cagcttttac atggcaatga caaagactct gtattgttgg
     1501 gaaattcccg gggcagtaaa gaggctcggg ccagggccag tgcagcactc cacaacatca
     1561 ttcactcaca gcctgatgac aagagaggca ggcgtgaaat ccgagtcctt catcttttgg
     1621 aacagatacg agcttactgt gaaacctgtt gggagtggca ggaagcccac gaacaaggca
     1681 tggaccagga caaaaaccca atgccagctc ctgttgagca tcagatctgt cctgctgtgt
     1741 gtgttctaat gaagctttca tttgatgaag agcataggca tgcaatgaat gaacttggta
     1801 ggaaggctac ccggggcatt tcatcccagg agctaggtca ggggctttca gggggactgc
     1861 aggccattgc agagttattg caggtggact gtgagatgta tgggcttact aatgaccact
     1921 acagtgttac tttaagacgg tatgctggaa tggctttgac aaacttgacc tttggagatg
     1981 ttgccaacaa ggctacgctg tgttctatga aaggctgcat gagagcactt gtggcccagt
     2041 taaaatctga gagtgaagac ttacagcagg ttattgcaag tgttttgagg aatttgtctt
     2101 ggcgagcaga tgtaaatagc aaaaagacgt tgagagaagt tggaagtgtg aaagcattga
     2161 tggaatgtgc tttggaagtt aaaaaggaat caaccctcaa aagcgttttg agtgccttat
     2221 ggaacctgtc tgcacactgc actgagaata aggctgacat ctgtgctgtg gatggagcac
     2281 tggcatttct ggttggcacc ctcacttacc ggagccagac aaatacttta gccattattg
     2341 aaagtggagg tgggatatta cggaatgtgt ccagcttgat agctacaaac gaagaccaca
     2401 ggcaaatcct aagagagaac aattgcctac aaactttatt acagcacttg aaatctcaca
     2461 gcttgacaat agtcagtaat gcatgtggaa ctttgtggaa tctctcagca agaaatccta
     2521 aagaccagga agccttgtgg gacatggggg cagtgagcat gctcaagaac ctcattcatt
     2581 ccaagcacaa aatgattgcc atgggaagtg cagcagcttt aaggaatctc atggcaaaca
     2641 gacctgcaaa gtataaggat gccaatatca tgtctcccgg ctcaagtctg ccatcccttc
     2701 acgttaggaa acagaaagct ctagaagctg agctagatgc tcagcattta tcagaaacct
     2761 tcgacaacat tgacaaccta agtcccaagg cctctcaccg gagtaagcag agacacaagc
     2821 agaatcttta tggtgactat gcttttgacg ccaatcgaca tgatgatagt aggtcagaca
     2881 atttcaatac tggaaacatg actgttcttt caccatattt aaatactacg gtattgccca
     2941 gctcttcttc ctcaagggga agtttagaca gttctcgttc tgagaaagac agaagtttgg
     3001 agagagagcg aggtattggc ctcagtgctt accatccaac aacagaaaat gcaggaacct
     3061 catcaaaacg aggtctgcag atcactacca ctgcagccca gatagccaaa gttatggaag
     3121 aagtatcagc cattcatacc tcccaggacg acagaagttc tgcttctacc accgagttcc
     3181 attgtgtggc agacgacagg agtgcggcac gaagaagctc tgcctcccac acacactcaa
     3241 acacatacaa cttcactaag tcggaaaatt caaataggac atgctctatg ccttatgcca
     3301 aagtggaata taaacgatct tcaaatgaca gtttaaatag tgtcactagt agtgatggat
     3361 atggtaaaag aggccaaatg aaaccctcag ttgaatccta ttctgaagat gatgaaagta
     3421 aattttgcag ttatggtcag tatccagctg acctagccca taagatacac agtgcaaatc
     3481 atatggatga taatgatgga gaactggata caccaataaa ttacagtctt aaatattcag
     3541 atgagcagtt gaactcagga aggcagagtc cctcacagaa tgaaaggtgg gcaagaccaa
     3601 agcatgtgat agaagatgaa ataaagcaaa acgagcaaag acaagcaaga agccagaaca
     3661 ccagttatcc tgtctattct gagaataccg atgacaaaca cctcaaattc caaccacatt
     3721 ttggacaaca agaatgtgtt tccccatata ggtcaagggg aaccagtggt tcagaaacaa
     3781 atcgaatggg ttctagtcat gcaattaatc aaaatgtaaa ccagtctctg tgtcaggaag
     3841 atgattatga agatgataaa cctaccaact acagtgaacg ttattctgag gaagaacaac
     3901 atgaagaaga agaagagaga ccgacaaatt atagcataaa atataatgaa gagaaacatc
     3961 atgtggatca gcctattgat tatagtttaa aatatgccac tgacatttct tcctcacaaa
     4021 aaccatcatt ttcattctca aagaattcat cagcacaaag cactaaacct gaacatctct
     4081 ctccaagcag cgagaataca gctgtacctc catctaatgc caaaaggcag aatcagctgc
     4141 gtccaagttc agcacaaaga aatggccaga ctcaaaaagg cactacttgc aaagtcccct
     4201 ccatcaacca agaaacaata cagacttact gcgtagaaga caccccaata tgtttttcaa
     4261 ggtgcagttc attatcatca ctgtcatcag ctgacgatga aataggatgt gatcagacaa
     4321 cacaggaagc agattctgct aatactctgc agacagcaga agtaaaagag aatgatgtaa
     4381 ctcggtcagc tgaagatcct gcaactgaag ttccagcagt gtcccagaat gctagagcca
     4441 aacccagccg actccaggct tctggcttat cttcagaatc aaccaggcat aataaagctg
     4501 ttgagttttc ttcaggagcc aagtctccct ccaaaagtgg tgctcagaca cccaaaagtc
     4561 ccccagaaca ctatgtccag gagactccgc tcgtattcag caggtgtact tctgtcagct
     4621 cccttgacag ttttgagagt cgctccattg ccagctctgt tcagagtgag ccatgtagtg
     4681 gaatggtgag tggcatcata agccccagtg accttccaga tagtcctggg cagaccatgc
     4741 caccaagcag aagcaaaacc cctccacctc ctccacagac agtgcaggcc aagagagagg
     4801 tgccaaaaag taaagtccct gctgctgaga agagagagag tgggcctaag cagactgctg
     4861 taaatgctgc cgtgcagagg gtgcaggtcc ttccagacgt ggatactttg ttacacttcg
     4921 ccacagaaag tactccagac gggttttctt gttcctccag cctaagtgct ctgagcctgg
     4981 atgagccatt tatacagaaa gatgtagaat taagaatcat gcctccagtt caggaaaacg
     5041 acaatgggaa tgaaactgaa tcagaacagc ctgaggaatc aaatgaaaac caggataaag
     5101 aggtagaaaa gcctgactct gaaaaagact tattagatga ttctgatgac gatgatattg
     5161 aaatattaga agaatgtatt atttcagcca tgccaacaaa gtcatcacgc aaagccaaaa
     5221 aactagccca gactgcttca aaattacctc cacctgtggc aaggaaacca agtcagctac
     5281 ctgtgtataa acttctgcca gcacagaata ggctgcaggc acaaaaacat gttagcttta
     5341 caccagggga tgatgtgccc cgggtgtact gtgtagaagg gacacctata aacttttcca
     5401 cagcaacgtc tctaagtgat ctgacaatag agtcccctcc aaatgaattg gctactggag
     5461 atggggtcag agcgggtata cagtcaggtg aatttgaaaa acgagatacc attcctacag
     5521 aaggcagaag tacagatgat gctcagcgag gaaaaatctc atctatagtt acaccagacc
     5581 tggatgacaa caaagcagag gaaggagata ttcttgcaga atgtatcaat tctgctatgc
     5641 ccaaaggaaa aagccacaag cctttccgag tgaaaaagat aatggaccaa gtccaacaag
     5701 catcctcgac ttcatctgga gctaacaaaa atcaagtaga cactaagaaa aagaagccta
     5761 cttcaccagt aaagcccatg ccacaaaata ctgaatatag aacgcgtgtg agaaagaata
     5821 cagactcaaa agttaatgta aatactgaag aaactttctc agacaacaaa gactcaaaga
     5881 aaccaagctt acaaaccaat gccaaggcct tcaatgaaaa gctacctaac aatgaagaca
     5941 gagtgcgggg gagcttcgcc ttggactcac cgcatcacta cacccctatt gaggggacgc
     6001 cgtactgctt ttcccgaaat gactccttga gttctctgga ttttgatgat gacgatgttg
     6061 acctttccag ggaaaaggcc gagttaagaa agggcaaaga aagcaaggat tccgaagcca
     6121 aagttacctg ccgcccagaa ccaaactcaa gccagcaggc agctagtaag tcacaagcca
     6181 gtataaaaca tccagcaaac agagcacagt ccaaaccagt gctgcagaaa cagcccactt
     6241 tcccccagtc ctccaaagac ggaccagata gaggggcagc aactgacgaa aaactgcaga
     6301 attttgctat tgaaaatact ccagtttgct tttctcgaaa ttcctctctg agttccctta
     6361 gtgacattga ccaggaaaac aacaataaca aagaaagtga accaatcaaa gaagctgaac
     6421 ctgccaactc acaaggagag cccagtaagc ctcaggcatc cgggtatgct cccaagtcct
     6481 tccacgtcga agacacccct gtctgtttct caagaaacag ctctctcagt tctcttagca
     6541 ttgactctga ggacgacctg ttacaggagt gtataagttc tgccatgcca aaaaagaaaa
     6601 ggccttcaag actcaagagt gagagcgaaa agcagagccc tagaaaagtg ggtggcatat
     6661 tagctgaaga cctgacgctt gatttgaaag atctacagag gccagattca gaacacgctt
     6721 tctcccccga ctcagaaaat tttgactgga aagctattca ggaaggcgca aactccatag
     6781 taagtagttt gcaccaagct gctgcagccg ccgcgtgctt atctagacaa gcgtcatccg
     6841 actcagattc cattctgtca ctaaagtccg gcatttctct gggatcgcct tttcatctta
     6901 cacctgatca agaggaaaag ccattcacaa gcaataaagg cccaagaatt ctcaaacctg
     6961 gagagaaaag cacattagaa gcaaaaaaaa tagaatctga aaacaaagga atcaaaggcg
     7021 ggaaaaaggt ttataaaagc ttgattacgg gaaagattcg ctccaattca gaaatttcca
     7081 gccaaatgaa acaacccctc ccgacaaaca tgccttcaat ctcaagaggc aggacgatga
     7141 ttcacatccc agggcttcgg aatagctcct ctagtacaag ccctgtctct aagaaaggcc
     7201 cacccctcaa gactccagcc tctaaaagcc ccagtgaagg gccgggagct accacttctc
     7261 ctcgaggaac taagccagca ggaaagtcag agcttagccc tatcaccagg caaacttccc
     7321 aaatcagtgg gtcaaataag gggtcttcta gatcaggatc tagagactcc actccctcaa
     7381 gacctacaca gcaaccatta agtaggccaa tgcagtctcc agggcgaaac tcaatttccc
     7441 ctggtagaaa tggaataagc cctcctaaca aactgtctca gctgcccaga acatcatctc
     7501 ccagtactgc ttcaactaag tcctccggtt ctgggaaaat gtcatataca tccccaggta
     7561 gacagctgag ccaacaaaat cttaccaaac aagcaagttt atccaagaat gccagcagta
     7621 tccccagaag tgagtcggca tctaaaggac tgaatcagat gagtaacggc aatgggtcaa
     7681 ataaaaaggt agaactttct agaatgtctt caactaaatc aagtggaagt gaatcagaca
     7741 gatcagaaag gcctgcatta gtacgccagt ctactttcat caaagaagcc ccaagcccaa
     7801 ccctgaggag gaaactggag gaatctgcct catttgaatc cctttctcca tcttctagac
     7861 cagattctcc caccaggtcg caggcacaga ccccagtttt aagcccttcc cttcctgata
     7921 tgtctctgtc cacacatcca tctgttcagg caggtgggtg gcgaaagctc ccgcctaatc
     7981 tcagccccac tatcgagtat aatgacggaa ggcccacaaa acggcatgat attgcacgct
     8041 cccattctga aagtccttcc agactaccaa tcaaccgggc gggaacctgg aagcgtgaac
     8101 acagcaaaca ttcctcgtcc cttcctcgag tgagtacttg gagaagaact ggaagctcat
     8161 cttctattct ttctgcttca tcagagtcca gtgaaaaagc aaaaagtgag gatgaaaggc
     8221 atgtgagctc catgccagca cccagacaga tgaaggaaaa ccaggtgccc accaaaggaa
     8281 catggaggaa aatcaaggaa agtgacattt ctcccacagg catggcttct cagagcgctt
     8341 cctcaggtgc tgccagtggt gctgaatcca agcctctgat ctatcagatg gcacctcctg
     8401 tctctaaaac agaggatgtt tgggtgagaa ttgaggactg ccccattaac aaccctagat
     8461 ctggacggtc ccccacaggc aacacccccc cagtgattga cagtgtttca gagaagggaa
     8521 gttcaagcat taaagattca aaagacaccc atgggaaaca gagtgtgggc agtggcagtc
     8581 ctgtgcaaac cgtgggtctg gaaacccgcc tcaactcctt tgttcaggta gaggccccag
     8641 aacagaaagg aactgaggca aaaccaggac agagtaaccc agtctctata gcagagactg
     8701 ctgagacgtg tatagcagag cgtacccctt tcagttccag tagctccagc aagcacagct
     8761 cacctagcgg gactgttgct gccagagtga caccttttaa ttacaaccct agccctagga
     8821 agagcagcgc agacagcact tcagcccggc cgtctcagat ccctacgcca gtgagcacca
     8881 acacgaagaa gagagattcg aagactgaca gcacagaatc cagtggagcc caaagtccta
     8941 aacgccattc cgggtcttac ctcgtgacgt ctgtttaaaa gagctagaag gacaaacgta
     9001 gaaaagctgg tgttaatcac tactgctgta tagagacttt atttcaaaga aaactttaaa
     9061 ggttgaaaat ttttgtaaat aggtttgatt cttgttagag gggttttgtt ctggaaacca
     9121 tatttgatag tacactttgt cctcactggt cattttggga ggcactctta ttaggtagga
     9181 aaaaatggta aagccaagta tatttgtaca gtgtgtttta catgtattta agtagcatcc
     9241 atcccattgt cctttaatta tcacctgtct taaaaataac actacagata gaaactatat
     9301 gatatattgc tgtcatcaat cacttttaga ttataaatta aacttacatc agggagaaat
     9361 tggtatttat gcaaaaaaaa ttagtccttg tgaatccatc taatgtcata attaatcatg
     9421 tggctgtgaa attcacagta atgtggttcc ccatgaatat gtttacccag cctgctttgc
     9481 tttactgcat gaatgaagct gacggttcaa tttcagaagt aacaattaat gattctgtga
     9541 tcacatggtg tgcagagaga tagctacagt gtaacgattc acactatttt gtgctcaaat
     9601 ctgtgtaact gtaaagcatt gaatgaaact attttaactg aactagattt tatcaaaagt
     9661 aggtagaatt tttgctatgc tgtaactgtt gtatattctg gtatttgagg tgagttggct
     9721 gctcttttat taatgagaca cgaattgtgt ctcaaactaa atgaacattt cagaataaat
     9781 tattgctgta tgtaaactat tactgaaatt ggtatttgtt tgagggtctt atttcacatt
     9841 tgtattaata actgtcaaaa gggcctcttt taaaagctaa atgtaatttt ttttcttcag
     9901 attctatgca ttaagagtaa aatttcctct tgctgtaata aagacaattg aagaagattg
     9961 ttgacactta accattccta aaatttattt ttatgtaatt agcacctctc aatgcttggt
    10021 atttttccac tttttttctt actccttggg ggtttgatta aaacaaatgc ctataatagc
    10081 acaagactag gcactggaca taagggccca catagaatat ctccttttcc ttaatagaat
    10141 ccatacttga aaaccattga ttcttgacag ttaccagttt tgtctttcag gctttccagt
    10201 gcaactgtct taccccgtca tcttctccgt attgatactg ggtctgaaat aaatgcctac
    10261 taccactctg tatagtaagg cagatttttt tttgcaatca ggcataatca gtattttctc
    10321 atactcaaaa aatacaagtc agtgaaacat cagagatatg taatgctgtt atgataatat
    10381 ttcaagaaag attcaaaagt gggccaggtc agtaggtaaa ggtgatgcct caaagcctga
    10441 aggcccagtt cacatcacag gatgcacatg gcagaaggaa agaaccagcc ccctaaagtc
    10501 atcctctgac ctccactcgc ctgtcttggc acatttgcat gtgtgcacac acagacttct
    10561 tacgttgtcc atgaggacat tcagggctaa cgagaaggct cagcaggtaa agcctggtga
    10621 catgaagtct gtccccagaa tcctccaacc tccacacacg ctccataatt agaaagtcac
    10681 aagttttttt ttaattccta cccgctaaag acaacttcat ttttaatggg tccggtggtt
    10741 gggatgttat taagtgccca ctgcccatat gaatgagaat acactgacat acttaatagc
    10801 caagagtttc agttcttgtc aaatgttatg tgaagttgtt ttatatcagt attccatatt
    10861 gttctccaag gttgccataa gcaatgggag atgggaatat aggtcacagc tggttctctt
    10921 tcattcctag ttttccatta gaattactgc acgtcagttt ttataaggca cttcaagtag
    10981 ttttgtggaa agagatgagg ttttagttag aatacaattg ccttttaatt catgttttaa
    11041 gaaattaatg ttagaagtaa aagaagagtc cattccacct attggagacg aaattcccat
    11101 gggcagccct gctgggaagg gtctgtcttg tgttgtccct tgaagatgga gttgctggat
    11161 taaacccttt ggctagagat aatggctgtg aaagcaaagg ggttgacctt agttgtgctt
    11221 tcagaagttc actggatgat tccttaactg gtagcaggcc aagtactcct ggattttgct
    11281 tgtttcagat ggcacactaa gcccagtcag tgttggggct tggttactgg gctggatgct
    11341 tattaaaagg gcaaggacct acttagcttt ggactgcagc aaagaaagcc taaggtagca
    11401 atttcaaaca ggtgtggttg gatcaccaga agtgtttgtt agatcatttc tgggcacaca
    11461 cccgcagttt gataagagtt gatctggaga gggctacaag aatttgcatc tgtaatgtat
    11521 ttagggatgg tgggtggtat gtgggcttct ggtgtgggga ccgtacttgg aaaagtactg
    11581 tcctaagtgt gtacagcaca gctcttaata agcaaatgag ttcctgatct accaagtaaa
    11641 tgctccttgg catgggagac ctgaatattg acttgtccag tctcctagcc tatgtcgact
    11701 cattgtaggg aggaaactca ccatacttag gaatacgaaa gtcaagatgt ccactgcctc
    11761 tgagtgcatc actcttgtcc ctttgaaata actactgtta aaagagggtg tgtgtggtgg
    11821 gggggggggt acttaaaata cttagtttcg atgggcagta agaggctatg gaaagggaaa
    11881 ttcaggatga gggaagattg gttgtaagtg aaaggaaact aatcaatttg ttagtacaca
    11941 gtagaagtga ttaattggtg aaaaaaatta tatttgataa tgaactttac aaattaccag
    12001 ggtactaact aggtaaataa ataaaagata agccctgtag gtatcaacca gcaataagta
    12061 atgttagagt tttctttata gtgtaaaatg tggatcatgt taccacaaag ttaattgaaa
    12121 gatgactttg tcactgaaaa tgggagatgg ttgccagggt ttctttgcag ctaagaaaat
    12181 gaaaaaatta aactgtccaa agctaaaggg tttacatggt cagataattc agatatacct
    12241 aacaactttt caggagtcaa ggctaaaata cagtgtatat tctttctcat tttctagtga
    12301 gcctatctat ttccctgtgt actaacctta gaaaacgact ttgtaatgtg gaattctatg
    12361 aaccgtgact tctgtaaacc caggtcattt tcctcctgtt ctaactctgc tttttgttag
    12421 gccattgatg catctttccg actgtatgtt ctgtgtataa tgctgttctt ggtgctaaat
    12481 agactttctt tcctctgata ttcaggataa tttttctcta tacttctgtt cacttgtttt
    12541 cttgaagtta tctgattgtt atttttaatt aaaaatactt aaatgta
//
