LOCUS       NM_144535               3111 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus adaptor-related protein complex 5, mu 1 subunit
            (Ap5m1), transcript variant 1, mRNA.
ACCESSION   NM_144535
VERSION     NM_144535.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Hirst,J., Barlow,L.D., Francisco,G.C., Sahlender,D.A., Seaman,M.N.,
            Dacks,J.B. and Robinson,M.S.
  TITLE     The fifth adaptor protein complex
  JOURNAL   PLoS Biol 9 (10), e1001170 (2011)
   PUBMED   22022230
  REMARK    Erratum:[PLoS Biol. 2012 Mar;10(3). doi:
            10.1371/annotation/89dff893-c156-44bb-a731-bfcc91843583]
REFERENCE   3  (bases 1 to 3111)
  AUTHORS   Lee,M.R., Shin,J.N., Moon,A.R., Park,S.Y., Hong,G., Lee,M.J.,
            Yun,C.W., Seol,D.W., Piya,S., Bae,J., Oh,J.W. and Kim,T.H.
  TITLE     A novel protein, MUDENG, induces cell death in cytotoxic T cells
  JOURNAL   Biochem Biophys Res Commun 370 (3), 504-508 (2008)
   PUBMED   18395520
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK016540.1, AK030928.1, AK164148.1 and AK051372.1.
            
            On Jan 26, 2018 this sequence version replaced NM_144535.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051372.1, AK030928.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1378              AK016540.1         2-1379
            1379-2314           AK030928.1         1360-2295
            2315-3074           AK164148.1         2140-2899
            3075-3111           AK051372.1         3077-3113
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 25.36 cM"
     gene            1..3111
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="adaptor-related protein complex 5, mu 1 subunit"
                     /db_xref="GeneID:74385"
                     /db_xref="MGI:MGI:1921635"
     exon            1..316
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..1715
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="MHD domain-containing death-inducing protein; muD;
                     mu-2-related death-inducing protein; adapter-related
                     protein complex 5 mu subunit; MU-2/AP1M2 domain
                     containing, death-inducing; adapter-related protein
                     complex 5 subunit mu-1; adaptor-related protein complex 5
                     subunit mu-1"
                     /codon_start=1
                     /product="AP-5 complex subunit mu-1"
                     /protein_id="NP_653118.3"
                     /db_xref="CCDS:CCDS26995.1"
                     /db_xref="GeneID:74385"
                     /db_xref="MGI:MGI:1921635"
                     /translation="MALRAVWLIRHEPGTPLGGTVRFSRRYPTVEKRAKAFNGMTYVP
                     VPEDGPFLRALLFQLRLLDDDKDFMERRDGCSRINKTSIYGLSVGGEELWPVIAFLRD
                     SMIYASVPLVEQALSPRPPLISISGVSQGLELLLGIQDFLYSSQKNDTDLHTKLSQLP
                     DLLLQACPLGTLLDANLQNSLNSINSVSVTQPQKQPAWKVGAYKGKAQISISITETVK
                     CMQYGKQDIADTWQVAGTVACKCDLEGVMPAVTISLSLPTNGSPLQDIIVHPCVTSLD
                     SAILTSSSIDTMEDSAFSGPYKFPFTPPLESFNLCHYTSQVPVPPILGSYHMKEEGVQ
                     LKVTVNFKLHESVRNNFEVCEAHIPFYNRGPITHLEYKASFGQLEVFREKSLLVWIIG
                     QKFPKSMEISLSGTLTFGVKGHNKQPFDHICIGNTAYIKLNFRIADYTLTGCYADQHS
                     VQVFASGKPKISAYRKLISSDYYIWNSKAPAPVTYASLLP"
     exon            317..962
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1190
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1330
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1416
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1535
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1632
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..3111
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2712..2717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="hexamer: AATAAA"
     regulatory      2729..2734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="hexamer: AATGAA"
     polyA_site      2734
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="major polyA site"
     polyA_site      2753
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="major polyA site"
     regulatory      3056..3061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="hexamer: ATTAAA"
     polyA_site      3078
                     /gene="Ap5m1"
                     /gene_synonym="4932432K03Rik; Mu5; Mudeng"
                     /note="major polyA site"
ORIGIN      
        1 attccagtcc cttgagattc cgggtgtagg ttcttaagac cttctgagga gtgttttaat
       61 ctcttgaagg gaccgggaga cttgagtcac ctcacccgga agagcgagca tctccagtga
      121 gctcttgcgg ctcagggaga acccggacgt tgcctggtac agcccatcta gaaggcgggg
      181 tctactttgg ctggatggcg accttagttt ctacatcgct ctgatacttg ggaggcttaa
      241 ccatggccct gcgggcagtg tggctcatac gtcacgaacc aggaactcca cttggaggca
      301 ccgtcagatt ctccagacga tacccaactg ttgaaaagcg agccaaagcc ttcaatggaa
      361 tgacttatgt tcctgtacct gaagatggcc cctttcttag agcattgctc tttcaactta
      421 gactattgga tgatgataag gacttcatgg agagacgtga tggctgctca cgcatcaata
      481 aaacatccat ctatgggctt tcagtaggag gtgaagagct ctggccagtc attgccttcc
      541 tgagggatag catgatatat gcaagtgttc cattagttga acaagctctg tcccctcgcc
      601 cacctttaat tagcattagt ggggtctcac aaggcttgga actgctgttg gggatacagg
      661 attttcttta ctcgagtcag aaaaatgaca ctgacctaca cacaaaactg agtcagttgc
      721 ctgacttgct tctacaggct tgtcctttgg gtactttatt ggatgctaac ttgcagaatt
      781 cactgaatag cattaattcc gtatctgtga ctcagccgca aaaacagcca gcctggaaag
      841 ttggagcata caaaggaaaa gcacagattt ctatttctat caccgaaacg gtaaaatgca
      901 tgcaatatgg taagcaggat atagcagaca catggcaagt tgccggaaca gtcgcttgca
      961 agtgtgactt ggaaggggtc atgccagctg ttaccatcag cttgagtctc cccaccaacg
     1021 ggtctccact tcaggatatt attgttcatc cctgtgtgac ttctctggat tctgccattc
     1081 tgacttctag tagcattgac acaatggagg attctgcatt tagtgggccg tataaatttc
     1141 cattcactcc gcctttagag tccttcaact tatgccacta cacttctcag gttcctgtcc
     1201 caccaatact gggttcttac cacatgaaag aagaaggagt acagctaaaa gtaacagtta
     1261 acttcaaact ccatgaaagt gtgagaaata attttgaagt ctgtgaagct cacatacctt
     1321 tttacaacag aggtccaatt acccatttgg aatacaaagc tagttttggt caacttgagg
     1381 tatttcgaga aaaaagctta ctggtctgga ttatcggcca gaagttcccc aaatcaatgg
     1441 aaattagtct ttctggaact ctaacttttg gagttaaggg ccataacaag cagccatttg
     1501 atcacatttg tattggaaat acagcgtata taaagcttaa ctttaggatt gcagattata
     1561 cactcactgg atgttatgca gaccagcatt cagttcaagt ttttgcatca ggaaagccaa
     1621 aaataagtgc atatcggaaa ctaatttctt ctgattatta catctggaat tctaaagcac
     1681 ctgctccagt aacatacgca tcattgttac catgatgttt ttcatattca aaaggattta
     1741 tatgatggta acattctatc atagaatcat agtatttatt tttttacatg tcttcccata
     1801 gcccatgact gaaaaattac tgagtggttt ctttatgaaa acattcatat cttacattta
     1861 tagtctgatt cagtgtaaaa gaatattttc aaggctaatg tccctttttg tcattgttac
     1921 gatgctctga tttcatgcca gtatgactca ggatatataa aagctgtgag tggcttcgct
     1981 ttagactagt ctttagtctc tgtaccacac gtcccttcct gtcccatgga gagagaaaca
     2041 gtgctgcaat cagagtccct tccttgagta agagctcagc cttctcctag tgcaggttca
     2101 gtcagccatc ctatgggctg taattgattt acaaggaaac tgtgtgctta tccaagcctc
     2161 agtacaaaga gcgtgctgtt gcagaatacc catggtctgg ttaaagtaat cacaaattgt
     2221 ttctttcaaa agagccagtg ggaaataatg ggataatgtt caaatagtag cctggttttt
     2281 atcatatctc tcctgctagc caataaacct ccatattcct atttgatgtt ctagaactcc
     2341 tgtctttctg agacagggtc tcataaagcc caggctggcc tgggtctcac tgtgtagcta
     2401 aggatgatcc ctcttaagtg ctgggattac agtggtatac caccatgtcc agtacccttc
     2461 taggactcga actcagggtt tcatgcctgc cagacaagca ccctcccagc tgagctgtga
     2521 ccctaaccca tcaaaggctg ctgtaacaaa ttcagttata tgccagaaca actgtgttta
     2581 ggatgccagt agacttaaga ctgtagtaac ttcagaagat gagcataatg atctatgatt
     2641 ctttagtgtc cttagacaag gctttaaaat aattgtaggt attatagtgt ttgtttagtt
     2701 tttaaaaata aaataaactc tgttaatgaa tgaactcagg taaacaacca aaagcctttc
     2761 tgtgggtatc atttagactt atttaatata gcaatttttt tctctttaag tctgtccctg
     2821 tcgttttctt tttaccattt tcttgattcc tttaaaactg tctagccaag ttccttatct
     2881 tctctcattt ctacaattac atgacatatt ctttttttag agcagcatac tgtcatcaaa
     2941 ttgaacttaa agtgactatt taaaaaaaaa aaaagatcta aagatcctgg gaacttaact
     3001 gatgccaaat ttagaagcca gacaataaat atgaattgta gaataatatt ttttaattaa
     3061 acttattctg ggttctgctt ctttttgtct tttttttccc cttgagtctt c
//
