LOCUS       NM_175550               6524 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus adaptor-related protein complex AP-4, epsilon 1
            (Ap4e1), mRNA.
ACCESSION   NM_175550 XM_894489 XM_901599 XM_901601 XM_901604 XM_918064
            XM_925455 XM_925461 XM_991402 XM_991421 XM_991442 XM_991461
VERSION     NM_175550.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Orlowski,A., Karippaparambil,J., Paumier,J.M., Ghanta,S.,
            Pallares,E., Chandran,R., Edmison,D., Tandukar,J., Gao,R. and
            Gowrishankar,S.
  TITLE     Axonal organelle buildup from loss of AP-4 complex function causes
            exacerbation of amyloid plaque pathology and gliosis in Alzheimer's
            disease mouse model
  JOURNAL   eNeuro 11 (12) (2024) In press
   PUBMED   39632089
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6524)
  AUTHORS   Caracci,M.O., Pizarro,H., Alarcon-Godoy,C., Fuentealba,L.M.,
            Farfan,P., De Pace,R., Santibanez,N., Cavieres,V.A., Pastor,T.P.,
            Bonifacino,J.S., Mardones,G.A. and Marzolo,M.P.
  TITLE     The Reelin receptor ApoER2 is a cargo for the adaptor protein
            complex AP-4: Implications for Hereditary Spastic Paraplegia
  JOURNAL   Prog Neurobiol 234, 102575 (2024)
   PUBMED   38281682
  REMARK    GeneRIF: The Reelin receptor ApoER2 is a cargo for the adaptor
            protein complex AP-4: Implications for Hereditary Spastic
            Paraplegia.
            Erratum:[Prog Neurobiol. 2024 Oct;241:102662. doi:
            10.1016/j.pneurobio.2024.102662. PMID: 39294072]
REFERENCE   4  (bases 1 to 6524)
  AUTHORS   Park,D., Wu,Y., Wang,X., Gowrishankar,S., Baublis,A. and De
            Camilli,P.
  TITLE     Synaptic vesicle proteins and ATG9A self-organize in distinct
            vesicle phases within synapsin condensates
  JOURNAL   Nat Commun 14 (1), 455 (2023)
   PUBMED   36709207
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6524)
  AUTHORS   Davies,A.K., Alecu,J.E., Ziegler,M., Vasilopoulou,C.G., Merciai,F.,
            Jumo,H., Afshar-Saber,W., Sahin,M., Ebrahimi-Fakhari,D. and
            Borner,G.H.H.
  TITLE     AP-4-mediated axonal transport controls endocannabinoid production
            in neurons
  JOURNAL   Nat Commun 13 (1), 1058 (2022)
   PUBMED   35217685
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6524)
  AUTHORS   Thomas,J.W. and Green,E.D.
  CONSRTM   NISC Comparative Sequencing Program
  TITLE     Comparative sequence analysis of a single-gene conserved segment in
            mouse and human
  JOURNAL   Mamm Genome 14 (10), 673-678 (2003)
   PUBMED   14694903
REFERENCE   7  (bases 1 to 6524)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 6524)
  AUTHORS   Hirst,J., Bright,N.A., Rous,B. and Robinson,M.S.
  TITLE     Characterization of a fourth adaptor-related protein complex
  JOURNAL   Mol Biol Cell 10 (8), 2787-2802 (1999)
   PUBMED   10436028
REFERENCE   9  (bases 1 to 6524)
  AUTHORS   Dell'Angelica,E.C., Mullins,C. and Bonifacino,J.S.
  TITLE     AP-4, a novel protein complex related to clathrin adaptors
  JOURNAL   J Biol Chem 274 (11), 7278-7285 (1999)
   PUBMED   10066790
REFERENCE   10 (bases 1 to 6524)
  AUTHORS   Odorizzi,G., Cowles,C.R. and Emr,S.D.
  TITLE     The AP-3 complex: a coat of many colours
  JOURNAL   Trends Cell Biol 8 (7), 282-288 (1998)
   PUBMED   9714600
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY037321.1, AK144636.1, AK036944.1 and AL928590.10.
            
            On or before Feb 7, 2007 this sequence version replaced
            XM_918064.2, XM_925461.2, XM_925455.2, XM_894489.2, XM_901604.2,
            XM_901599.2, XM_901601.2, XM_991421.1, XM_991461.1, XM_991402.1,
            XM_991442.1, NM_175550.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144636.1, AK036944.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                BY037321.1         2-22
            22-909              AK144636.1         2-889
            910-4195            AK036944.1         891-4176
            4196-6524           AL928590.10        41593-43921
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.76 cM"
     gene            1..6524
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /note="adaptor-related protein complex AP-4, epsilon 1"
                     /db_xref="GeneID:108011"
                     /db_xref="MGI:MGI:1336993"
     exon            1..190
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..3412
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /note="epsilon-adaptin; epsilon subunit of AP-4; AP-4
                     adapter complex subunit epsilon; adapter-related protein
                     complex 4 subunit epsilon-1; AP-4 adaptor complex subunit
                     epsilon; adaptor-related protein complex 4 subunit
                     epsilon-1"
                     /codon_start=1
                     /product="AP-4 complex subunit epsilon-1"
                     /protein_id="NP_780759.2"
                     /db_xref="CCDS:CCDS38231.1"
                     /db_xref="GeneID:108011"
                     /db_xref="MGI:MGI:1336993"
                     /translation="MSDMVERTLTALPGLFLQNQLGGPAASRAPFFSRLGGLIRGVTA
                     LSSKHEEEKLIQQELSSLKATVSAPTTTLKTMKECMVRLIYCEMLGYDASFGYIHAIK
                     LAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQIF
                     PREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFYLIAPNQVQHIHTKFRKALCDRDV
                     GVMAASLHIYLRMIKENASGYKDLTESFVTILKQVVGGKLPVEFSYHSVPAPWLQIQL
                     LRILGLLGKDDERTSELMYDVLDESLRRAELNHNVTYAILFECVHTIYSIYPKSELLE
                     KAAKCIGKFVLSPKINLKYLGLKALTYVIQQDPSLALQHQITIIECLDHPDPIIKRET
                     LELLYRITNAQNVVVIVQKMLEYLHQSKEEHIIISLVGRIAELAEKYAPDNVWFIQTM
                     NAVFSVGGDVMHPDILSNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDMENTFYPQR
                     FLQVMSWVLGEYSYLLDKESPEEVITRLYKLLMSDSISSETKAWLFAAVTKLTPQAHS
                     SPLVEKLIQEFTVSLNTCLRQHAFELKHLHENTELMKSLLQGAQNCEDIVADASLSFL
                     DGFVAEGLSQGAAPYKPHHQRQEEQLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLG
                     SDISGNSAETGLKETSSLKMEGIKKLWGKEGYLPKKESGTGDKPEASHVPAEGATVEN
                     VDQATTRKDQAQGHIPSTEEKEKQLLASSLFVGLGPENTVDLLGKADVVSHKFRRKSK
                     LKVAQSDKTPSAPTAPCSALSLGSDVAGGDEDGLSAVDRGDGELSSELFRSESLSGPP
                     SAEKLESVSLPVPSLFADNNMEVFNPPSSSATSTVKEETPECRHSGLVEICSNEAVSV
                     SSYKVWRDDCLLVIWAVTSKTDSEFTDAQLEIFPVENFKIIEQPECSSPVIETERTKS
                     FQYSVQMESPCIEGTLSGFIKYQMMDTHSVQLEFSMNLPLLDFIRPLKISTEDFGKLW
                     LSFANDVKQTIKISEPGVALTSVLTELQQNLRLRVIDVIGNEGLLACKLLPSTPCVLH
                     CRVHADAVALWFRSSSSVLSDYLSCHCQKVMQTS"
     exon            191..262
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            263..386
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            387..460
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            461..582
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            583..742
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            743..909
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            910..983
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1106
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1216
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1356
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1469
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1588
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1891
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2006
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2130
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2389
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2902
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3093
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3251
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..6524
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6105..6110
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      6125
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /note="major polyA site"
     regulatory      6203..6208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
                     /note="hexamer: AATACA"
     polyA_site      6213
                     /gene="Ap4e1"
                     /gene_synonym="2310033A20Rik; 9930028M04Rik"
ORIGIN      
        1 ggccgggaga gtcagcgcga agtagcctcg tagggaggca gcgatgagcg acatggtgga
       61 gaggacgctg accgcactgc cggggctctt cctgcagaac cagctgggag gacctgcggc
      121 ctccagagca cccttcttct ccaggctggg cggcctcatc cgtggcgtca ctgcactctc
      181 atccaagcac gaagaagaga aattaatcca gcaggaactg agtagtctga aggcaactgt
      241 ttctgctccg accacgacac tgaaaacgat gaaggaatgt atggtgagac ttatatactg
      301 tgagatgctt ggatacgatg cttcttttgg ttatatacat gcaatcaagt tggcccaaca
      361 aggaaacctc ttagaaaaaa gagtgggtta tttggctgtt tctttatttc tacatgaaag
      421 tcatgaactt ttgcttctcc ttgtgaatac agtggtaaag gacctgcaga gcactaatct
      481 ggtagaagta tgtatggcac ttactgttgt cagccagatt ttccctcgag aaatgattcc
      541 agccgttctt ccactaatag aagataaact tcaacattct aaggagatta tacgaagaaa
      601 agcagttctg gcattataca aattttacct cattgccccc aatcaagtac agcacatcca
      661 cactaaattt cggaaagcgc tttgtgacag agatgttggg gtcatggctg cctctttgca
      721 catatacctt aggatgatta aggaaaatgc ttctggctat aaagacttga cggagagttt
      781 tgtaacaatt ttaaagcagg tggtgggcgg gaagctcccg gtagagttca gttaccacag
      841 cgtgccagca ccatggttac agattcagct cctgagaata ctgggactgc tggggaaaga
      901 cgatgaaagg acaagtgaat taatgtatga tgttcttgat gaatccttac gaagagccga
      961 gttaaatcac aatgtcactt acgctattct gtttgaatgt gtacacacaa tctattctat
     1021 ttatcctaag tcagaattac ttgagaaggc tgccaagtgc attggaaaat ttgttctgtc
     1081 acctaaaata aacctcaaat atttaggact gaaggctctt acctatgtta tccagcagga
     1141 tccttctctg gctcttcaac accagataac cataattgaa tgcctagacc accctgatcc
     1201 cattattaaa agagagactc tggaacttct ttacagaatt acaaatgcac agaatgtagt
     1261 ggttattgta cagaaaatgc ttgaatactt acatcagagc aaagaagagc atatcatcat
     1321 cagtttagtt ggcagaattg ctgagctggc tgagaaatat gctcctgaca acgtgtggtt
     1381 tattcagaca atgaatgctg tgttttcagt gggaggagat gtaatgcatc cagatattct
     1441 cagcaacttc ctgagactgc tagcagaagg ttttgatgat gaaacagaag atcaacaatt
     1501 aagactctac gcagttcagt cttatcttac tttactagat atggaaaata ctttctatcc
     1561 acagagattt cttcaagtta tgagttgggt gttaggagag tactcctacc tcttagataa
     1621 agaaagtcca gaggaggtta taaccaggct ctacaagtta cttatgagtg actccatttc
     1681 ttcagaaacg aaagcatggt tgtttgctgc ggtaaccaag ttgacacctc aagctcattc
     1741 ttctccctta gttgagaaat taatccaaga attcactgta tccttgaata cttgtctgag
     1801 acagcatgca tttgaattga aacatctgca tgaaaataca gagcttatga agagcctgct
     1861 tcagggtgct cagaattgtg aagacatagt ggcagatgcg tctttatctt ttctggatgg
     1921 ttttgtggct gaaggactca gtcagggagc agcaccttat aagccccatc accagcgcca
     1981 agaggaacag ctctctcagg aaaaggttct caattttgaa ccatatggac tatcattttc
     2041 ttcatctggc ttcactggac gacagtctcc ggctggcatt tccctgggct ctgatatatc
     2101 tggaaacagt gccgagacag ggctgaaaga gacaagcagt ttgaagatgg aagggataaa
     2161 gaaattatgg ggtaaagagg gctatctgcc caagaaggag agtggaactg gtgataaacc
     2221 ggaagcctca catgttcctg cagagggtgc aacagtggag aatgtagacc aagccacgac
     2281 cagaaaggac caagctcaag gccatatccc ttctacagag gagaaagaaa agcagctgtt
     2341 ggcatcatcg ctgtttgttg ggctaggacc agaaaataca gtcgatttgt tgggaaaagc
     2401 agatgttgtg tctcacaagt tcaggaggaa atcaaaactc aaggtagccc aaagcgacaa
     2461 gacacccagc gctcctactg ctccctgctc cgccctcagc cttggctcag atgtggctgg
     2521 tggggatgag gacggtctga gtgctgtgga tagaggggac ggagaactca gttcagagct
     2581 ttttcgttct gagtctctct cggggccgcc ctcagctgag aagctcgaga gtgtcagctt
     2641 gcctgtgcct tctttatttg ctgataacaa catggaagtc tttaatcccc cttcatcctc
     2701 tgcaacttca actgtcaagg aagaaactcc tgagtgcagg cattcaggtc tcgtggaaat
     2761 ctgcagtaat gaagccgtgt ctgtgtcttc ttacaaagtc tggagggatg attgtttatt
     2821 ggtgatctgg gcagtcacta gtaaaactga ctcagagttc acagatgctc agttagaaat
     2881 ttttcctgtg gaaaatttca agatcattga gcaacctgaa tgttcttctc ctgtgataga
     2941 aacagaacgc accaaatcct ttcaatacag tgtgcagatg gaaagccctt gtatagaggg
     3001 gactctctct ggttttataa agtatcaaat gatggatacg cattctgttc aactggaatt
     3061 ttccatgaac ttaccattat tagattttat tagaccatta aagatctcaa ctgaagactt
     3121 tggcaaactc tggttgtcct ttgcaaatga tgtgaagcaa actataaaaa tatcagagcc
     3181 tggagttgct ctgacttctg tcctaacaga gctgcagcag aacctgagac ttcgtgtgat
     3241 tgacgtcata ggcaatgaag ggctgttggc ctgtaagctg ctcccatcca ccccctgtgt
     3301 gctgcactgc cgagtacatg ctgacgcggt agctctgtgg ttccggtcct ctagctctgt
     3361 tctttcagac tatttatcgt gtcactgtca aaaagtgatg cagacatcct agcgaagttg
     3421 tgttaaattt taccttttcc acataaatgg tttacatcta taaccttacc aaagtaaaaa
     3481 gcactcgtgg tacttgtgat ggaggtgggg ggctgcaagt tttggtttat gtggtttctt
     3541 cttgagtcct gctaagaatg atccccaaga aactgtgtca tcaatttttt tactcaggat
     3601 tgtacagtat attccccccc ccacatgacc taagctaata ttataaaatg ttaatgattt
     3661 tatgtcactt aatttggagg gactgaaaat atttattttg ttataaatgt tttatagcag
     3721 gtttattcta gcattaactg atttctaata aggctgagac tcagttctct gctttggtgc
     3781 aggggacaca gagactgctc atcacctgaa tcacacttgg ctttggagtg cagaacctgc
     3841 tgtaaggaaa atctgtgctc tcggctctgc tttgcacagg tcagggttaa gctgaggcgg
     3901 cctaacacac gctaaccatt gtacaagacc atggcatgct ggctgttagg caaacacaat
     3961 ctctggcttt tactttttaa cttgttacac ttaattttca gtaatttgac cataaagtat
     4021 atctgtttgc tattagaccc tttttgcagt taaaatttgt gatcatagta taaatggcca
     4081 attgattatt ttttttctgg tccttaaatc tactagtatt aagtggatgt tactgagtag
     4141 tgactatgta aatatatcct acaaatactt cggcaaaata tatcatttaa atgttatatt
     4201 tatgcttcta ggccctctat tagtaaaaga agagattatg ccttgtgtgt gtgatgggcc
     4261 agaatcttga gattgtatat taactgaaat agtattttat aaaaactaat ggtgctgtgg
     4321 gaaatattta ttttaccctt tttggtactt caccattgaa actattcagg taatacaact
     4381 taagttattc ttagtggtca ggcactctgg ttttacgtta tctcaccaat acttgctgta
     4441 aagatgctga ggaggaggcc aggtggtggt ggtggtggtg atgcacgcct ttaatcccag
     4501 cactcgggag gcagaggcag gcggatttct gagttcgagg ccagcctggt ctacagagtg
     4561 agttctagga cagtcagggc tacacagaga aaccctgtct caaaaaaaaa aaaaaaaaag
     4621 attctacaga gctgtcatcc atagttagaa caagacaaaa agttatattt tttgtatttt
     4681 ttaagttaac ttttaagata gtcattcata aatgtagcat tttgttaacg tcgctcttaa
     4741 atacatgcag ttaagccttg ctgagagagg agggtgacgg aatgtcctgc actttgggtt
     4801 tctcccttca gaagaaggaa gactggatgc atcagcagcc tcggcactgt cgggatagag
     4861 ggtttggaag cagctgctcc attagcttgc tcttcctctt tatgttgcca ttcccagcct
     4921 tccctgctcc ttacccaggc tcgccctgac tttatcaagc agtgcggcca tgtggtgtag
     4981 gacgggctcc tttgtgtatt ttttcaggcg tttctgtggt cactgagttt ggtcagttgt
     5041 ggagcaggga gagtaaggta tataaaatca cagtgacatc tgtccgaagg atcatggttt
     5101 ttgctttctt ctgctttgtt ataagggatg gttatgagag gactctactg gcctgtctaa
     5161 cagcgcatgt gtaaacagta ggtttggggt tgaggcatga cttcagatct gccaaagaga
     5221 acatagtgtc acttttgttc tttctgaact cattaaataa aatgtagagt aacagggttt
     5281 ctacagaaat gtttaataca atgtttacaa tgtttatgat tttaataaac taataataat
     5341 agaaaagagt agatatttta aaactttgga ccagtacata ttctatctaa actcactgca
     5401 ttatattctg aatcaggcat cgtcaacctt tttgatcaca aaatagggag aattttaggt
     5461 ctagttcttg cgtggggcca tccataacac ctagtctctt tactttataa tcagccaatg
     5521 agggtagcgc ttaggggaag aatggctgtg tgctgtgtgc cgggcaccat taccaagaat
     5581 ggagggcagc agagccaggc tggggacaga taaaggagga caggaagagc atgagagcag
     5641 cggaaaggag gtggccaggc tcctagcagt cacccaggcg ctcagcagaa gagcggccac
     5701 tcagggtatg agaggagcct gtcttaccct tccctacaga gaagctgtgg gaggagctga
     5761 gccatctgag agccagtgta caggtgtgcc cagcgcagca tgtgctctgc aggggagtgt
     5821 tggctgacgt gctaagctct tccaaattga cttataaaag tacagtattt tattttcatt
     5881 taactgtagg ttcttttatt ttctatggct aaacatgatc attgtcacat ggtaagccat
     5941 aagaagtgca ctgaccctat tcatgtgtct ttgagctgtt ttcttatgtc agaactttag
     6001 gagagaacaa tgcaagtgaa tagcaacgta ataaatgatg catttttttt ttactttgtt
     6061 ttggcactgg actgtgtaaa ttgaaatttt ttaattgaat gtataataaa aacatctgtg
     6121 ctttttgggg tctgtatgtt ctttataatt taagtagatg aaaattattt aatcagtata
     6181 tagataggtt tctaaatata taaatacaac ttgctcagtc tgtatgtttg tttttgcatg
     6241 tgtgttatat gcacatgttt gtgtggctgt acatataatc atgtaaatgt tcatgtttca
     6301 gaggccagag atcaacatca ggtttcttcc tcactatctt attagttttt aacttttaaa
     6361 aatatggctt attcatacat aggtgcttgg tgctagcaga ggtcagaaga gccatcggtt
     6421 cccctggaac tagagttaaa gaacaaatgg ttgtgagctg ccgtgtgagt gcaggaaatt
     6481 gaacccaggt gctctgcagg ttctacagca caacaagagc tctt
//
