LOCUS       NM_009679               2937 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus adaptor-related protein complex 2, mu 1 subunit
            (Ap2m1), transcript variant 1, mRNA.
ACCESSION   NM_009679 XM_358784
VERSION     NM_009679.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Camblor-Perujo,S., Ozer Yildiz,E., Kupper,H., Overhoff,M.,
            Rastogi,S., Bazzi,H. and Kononenko,N.L.
  TITLE     The AP-2 complex interacts with gamma-TuRC and regulates the
            proliferative capacity of neural progenitors
  JOURNAL   Life Sci Alliance 7 (2), e202302029 (2023)
   PUBMED   38086550
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Lopez-Hernandez,T., Takenaka,K.I., Mori,Y., Kongpracha,P.,
            Nagamori,S., Haucke,V. and Takamori,S.
  TITLE     Clathrin-independent endocytic retrieval of SV proteins mediated by
            the clathrin adaptor AP-2 at mammalian central synapses
  JOURNAL   Elife 11, e71198 (2022)
   PUBMED   35014951
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2937)
  AUTHORS   Coccia,E., Planells-Ferrer,L., Badillos-Rodriguez,R., Pascual,M.,
            Segura,M.F., Fernandez-Hernandez,R., Lopez-Soriano,J., Gari,E.,
            Soriano,E., Barneda-Zahonero,B., Moubarak,R.S., Perez-Garcia,M.J.
            and Comella,J.X.
  TITLE     SIVA-1 regulates apoptosis and synaptic function by modulating XIAP
            interaction with the death receptor antagonist FAIM-L
  JOURNAL   Cell Death Dis 11 (2), 82 (2020)
   PUBMED   32015347
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2937)
  AUTHORS   Fred,S.M., Laukkanen,L., Brunello,C.A., Vesa,L., Goos,H.,
            Cardon,I., Moliner,R., Maritzen,T., Varjosalo,M., Casarotto,P.C.
            and Castren,E.
  TITLE     Pharmacologically diverse antidepressants facilitate TRKB receptor
            activation by disrupting its interaction with the endocytic adaptor
            complex AP-2
  JOURNAL   J Biol Chem 294 (48), 18150-18161 (2019)
   PUBMED   31631060
REFERENCE   5  (bases 1 to 2937)
  AUTHORS   Azarnia Tehran,D., Lopez-Hernandez,T. and Maritzen,T.
  TITLE     Endocytic Adaptor Proteins in Health and Disease: Lessons from
            Model Organisms and Human Mutations
  JOURNAL   Cells 8 (11), 1345 (2019)
   PUBMED   31671891
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2937)
  AUTHORS   Collins,B.M., McCoy,A.J., Kent,H.M., Evans,P.R. and Owen,D.J.
  TITLE     Molecular architecture and functional model of the endocytic AP2
            complex
  JOURNAL   Cell 109 (4), 523-535 (2002)
   PUBMED   12086608
REFERENCE   7  (bases 1 to 2937)
  AUTHORS   Jiang,C.H., Tsien,J.Z., Schultz,P.G. and Hu,Y.
  TITLE     The effects of aging on gene expression in the hypothalamus and
            cortex of mice
  JOURNAL   Proc Natl Acad Sci U S A 98 (4), 1930-1934 (2001)
   PUBMED   11172053
REFERENCE   8  (bases 1 to 2937)
  AUTHORS   Odorizzi,G., Cowles,C.R. and Emr,S.D.
  TITLE     The AP-3 complex: a coat of many colours
  JOURNAL   Trends Cell Biol 8 (7), 282-288 (1998)
   PUBMED   9714600
  REMARK    Review article
REFERENCE   9  (bases 1 to 2937)
  AUTHORS   Ohno,H., Poy,G. and Bonifacino,J.S.
  TITLE     Cloning of the gene encoding the murine clathrin-associated adaptor
            medium chain mu 2: gene organization, alternative splicing and
            chromosomal assignment
  JOURNAL   Gene 210 (2), 187-193 (1998)
   PUBMED   9573360
REFERENCE   10 (bases 1 to 2937)
  AUTHORS   Ohno,H., Stewart,J., Fournier,M.C., Bosshart,H., Rhee,I.,
            Miyatake,S., Saito,T., Gallusser,A., Kirchhausen,T. and
            Bonifacino,J.S.
  TITLE     Interaction of tyrosine-based sorting signals with
            clathrin-associated proteins
  JOURNAL   Science 269 (5232), 1872-1875 (1995)
   PUBMED   7569928
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK146517.1 and AK141862.1.
            
            On Dec 10, 2014 this sequence version replaced NM_009679.2.
            
            Summary: This gene encodes the mu subunit of the clathrin adaptor
            protein complex AP-2. It mediates sorting of cargo proteins
            harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate
            splicing of this gene results in multiple transcript variants.
            Pseudogenes of this gene are found on chromosomes 2, 8 and 19.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK141862.1, SRR1660819.133651.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                AK146517.1         1-69
            70-2937             AK141862.1         1-2868
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 12.46 cM"
     gene            1..2937
                     /gene="Ap2m1"
                     /note="adaptor-related protein complex 2, mu 1 subunit"
                     /db_xref="GeneID:11773"
                     /db_xref="MGI:MGI:1298405"
     exon            1..122
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            123..239
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1473
                     /gene="Ap2m1"
                     /note="isoform 1 is encoded by transcript variant 1; AP-2
                     complex subunit mu; mu2-adaptin; AP-2 mu chain; clathrin
                     coat assembly protein AP50; clathrin coat-associated
                     protein AP50; adaptor protein complex AP-2 subunit mu;
                     plasma membrane adaptor AP-2 50 kDa protein;
                     adapter-related protein complex 2 mu subunit; clathrin
                     assembly protein complex 2 medium chain; adaptor-related
                     protein complex AP-2, mu1; clathrin-associated AP-2;
                     adaptor protein complex AP-2, mu1; clathrin assembly
                     protein complex 2 mu medium chain; adaptor-related protein
                     complex 2 subunit mu"
                     /codon_start=1
                     /product="AP-2 complex subunit mu isoform 1"
                     /protein_id="NP_033809.1"
                     /db_xref="CCDS:CCDS28048.1"
                     /db_xref="GeneID:11773"
                     /db_xref="MGI:MGI:1298405"
                     /translation="MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVR
                     SPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIK
                     NNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIG
                     WRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMND
                     KIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMR
                     YRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQ
                     VICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEV
                     PFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC"
     exon            240..505
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            506..588
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            589..594
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..730
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            731..872
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            873..992
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1128
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1226
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1338
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..2937
                     /gene="Ap2m1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2058..2063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2m1"
                     /note="hexamer: ATTAAA"
     polyA_site      2076
                     /gene="Ap2m1"
                     /note="major polyA site"
     regulatory      2655..2660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2m1"
                     /note="hexamer: AATAAA"
     polyA_site      2691
                     /gene="Ap2m1"
ORIGIN      
        1 ggcggggtgg ggcgggagca ctgcagtgaa agccgaggca gcagacaggc gagctggggg
       61 cgggcggaca tcttgggatc cgagagtggc caggccggcc ttgtgggggc tgctgactcc
      121 aggtctgttc tcagagcaat gggtcacaga gactgagctg ccaccatgat cggaggctta
      181 ttcatctata atcacaaggg ggaggtgctt atctcccggg tctacagaga tgacatcggg
      241 aggaatgctg tggatgcctt tcgggtcaat gtcattcatg cacggcagca ggtgcgcagc
      301 cctgtcacaa acatcgctcg caccagcttc ttccatgtta agcggtccaa catctggctg
      361 gccgcagtca ccaagcagaa tgtcaatgct gccatggtct tcgaattcct ctacaagatg
      421 tgtgatgtaa tggctgctta ctttggcaaa atcagcgagg agaacatcaa gaacaatttt
      481 gtgctcatat acgagctgct ggatgagatt ctggactttg gctacccaca gaactcagag
      541 acaggtgcac tgaaaacctt catcacccag cagggtatca agagtcagca tcagacgaag
      601 gaagaacagt cccagatcac cagccaggtg accgggcaga ttggctggcg gcgagaaggc
      661 atcaagtatc gccggaatga actcttccta gatgttctgg agagtgtgaa cctgcttatg
      721 tccccacagg ggcaggtgct gagtgcccat gtgtcaggcc gggtggtgat gaagagttac
      781 ctgagtggca tgcctgagtg caagtttgga atgaatgaca agattgtcat agaaaagcag
      841 ggcaaaggca cagctgatga aacaagcaag agtggtaagc agtcgatcgc cattgatgac
      901 tgcaccttcc accagtgtgt gcgactcagc aagtttgact ctgagcggag catcagcttc
      961 atccctcccg acggagagtt tgaactcatg agataccgta ctaccaagga catcatcctt
     1021 cctttccggg tgatcccatt ggtgcgggag gtggggcgca ccaaactgga ggtcaaggtg
     1081 gtcatcaagt ccaacttcaa gccctcactt ctggcccaga agattgaggt gaggatcccg
     1141 actccattga acacaagcgg ggtacaggtg atctgcatga aggggaaggc caagtacaag
     1201 gccagcgaga acgccattgt atggaagatc aagcgcatgg caggcatgaa ggaatcccag
     1261 atcagcgcag agattgagct cctgcccacc aacgataaga agaaatgggc tcggcccccc
     1321 atttccatga actttgaggt gccattcgcg ccttctggcc tcaaggtgcg ctacttgaag
     1381 gtattcgaac cgaagctgaa ctacagcgac catgatgtca tcaaatgggt gcgatacatt
     1441 ggccgcagtg gcatttatga aacccgctgc tagctgccta gttgcagctg gcccacctcc
     1501 ctacccaccc ttcccacagg tccaagtacc cctagtgacc actcaccagt gtcgttcctc
     1561 cgtcctgctc tgctgccttt cctttccaat agcccctgag tataagtctg aaccagccac
     1621 accacagtgg aactggtcag gtagtgcctg gcttcctggg agggtggttt ttgaggcttc
     1681 tcttccgtct gtctgtcctg gcttagcgcc aggccctgag ttttgtgacc aaagccaggt
     1741 gctctcctct tctctctacc cctgtggcca cacctctgca gtgggaaggt tgactacccc
     1801 tcacctcaga gcttccccaa aggccagtaa cagatcccca gtctttagtc cccactctgc
     1861 tttgggatag tatgagcttc gttttgtaca cgtgtgactt tgtccagtta caaacccaat
     1921 aaacaataga gtggagttgc tggctaaatg ttaatggggc ccgcagaggg tctgacggga
     1981 ctattattac tccatgcagt tctggctgtc ctggaacctg tgtgtaggct caggagatct
     2041 acttcctgag tgctgagatt aaagtgtaca ccaccatgcc agactttgtt ttgtttgggt
     2101 cttgctgctt gtagctctgg ctgacctaga aatcactatg tagaccagac tgcccgtaaa
     2161 ttttaggtga tccctgtgcc tctgcttctc aagtgctgat actgcagata tgtgctttga
     2221 gatgggtttc attgtgtgag ctgaggtggc tggactcagg gtcctcccgc ctccatctct
     2281 caagtgcagt gttgtaagtg tgcaggtttc ccagttagta caaagaggca agatttgtat
     2341 tagttttgtt ttgttttgtt tttttaagga tttatatcag tacactgtag ctgttttcag
     2401 acacaccaaa agagggcatc agatcccttt agaggtggtt gtgagccact atgtggctgc
     2461 tggaaaatga actcaggacc tctggaagaa cagtcagtgc tctttaactg ctgagccatc
     2521 tctccaagat cttgtattag ttcttgcagg aaagatattt tccccatgca tcttatcaaa
     2581 ccctggcaaa aacctccatt cttccagagt tcctaagggg attgattgtc ccaaagatct
     2641 tgtatgttag aataaataaa gaatagggtt gccctcacca gttggacctt atgaggtttc
     2701 agctttttta aacaaacaaa caaaaactag agtaaattta aagcataaga atatagctaa
     2761 tcgaggctgg agagatggct cagtggttaa gagcactgac tgctcttcca gaggtcctga
     2821 gttcaattcc cagcacccac atggtggctc acaaccatct gcaatgtgat ctgacgccct
     2881 cttctggagt gtctgaagac agctatggtg tactcatata cataaaacaa ataaatc
//
