LOCUS       NM_027915               5369 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus adaptor-related protein complex 2, beta 1 subunit
            (Ap2b1), transcript variant 2, mRNA.
ACCESSION   NM_027915
VERSION     NM_027915.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5369)
  AUTHORS   Azarnia Tehran,D., Lopez-Hernandez,T. and Maritzen,T.
  TITLE     Endocytic Adaptor Proteins in Health and Disease: Lessons from
            Model Organisms and Human Mutations
  JOURNAL   Cells 8 (11), 1345 (2019)
   PUBMED   31671891
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5369)
  AUTHORS   Yuan,Y., Zhao,X., Wang,P., Mei,F., Zhou,J., Jin,Y., McNutt,M.A. and
            Yin,Y.
  TITLE     PTENalpha regulates endocytosis and modulates olfactory function
  JOURNAL   FASEB J 33 (10), 11148-11162 (2019)
   PUBMED   31291551
REFERENCE   5  (bases 1 to 5369)
  AUTHORS   Piccini,A., Castroflorio,E., Valente,P., Guarnieri,F.C., Aprile,D.,
            Michetti,C., Bramini,M., Giansante,G., Pinto,B., Savardi,A.,
            Cesca,F., Bachi,A., Cattaneo,A., Wren,J.D., Fassio,A., Valtorta,F.,
            Benfenati,F. and Giovedi,S.
  TITLE     APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle
            Trafficking and Neuronal Development
  JOURNAL   Cell Rep 21 (12), 3596-3611 (2017)
   PUBMED   29262337
REFERENCE   6  (bases 1 to 5369)
  AUTHORS   Rappoport,J.Z., Taha,B.W., Lemeer,S., Benmerah,A. and Simon,S.M.
  TITLE     The AP-2 complex is excluded from the dynamic population of plasma
            membrane-associated clathrin
  JOURNAL   J Biol Chem 278 (48), 47357-47360 (2003)
   PUBMED   14530274
  REMARK    GeneRIF: the AP-2 complex is not associated with the endocytosed
            plasma membrane-associated clathrin
REFERENCE   7  (bases 1 to 5369)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 5369)
  AUTHORS   Le Bras,S., Cohen-Tannoudji,M., Guyot,V., Vandormael-Pournin,S.,
            Coumailleau,F., Babinet,C. and Baldacci,P.
  TITLE     Transcript map of the Ovum mutant (Om) locus: isolation by exon
            trapping of new candidate genes for the DDK syndrome
  JOURNAL   Gene 296 (1-2), 75-86 (2002)
   PUBMED   12383505
REFERENCE   9  (bases 1 to 5369)
  AUTHORS   Collins,B.M., McCoy,A.J., Kent,H.M., Evans,P.R. and Owen,D.J.
  TITLE     Molecular architecture and functional model of the endocytic AP2
            complex
  JOURNAL   Cell 109 (4), 523-535 (2002)
   PUBMED   12086608
REFERENCE   10 (bases 1 to 5369)
  AUTHORS   Friocourt,G., Chafey,P., Billuart,P., Koulakoff,A., Vinet,M.C.,
            Schaar,B.T., McConnell,S.K., Francis,F. and Chelly,J.
  TITLE     Doublecortin interacts with mu subunits of clathrin adaptor
            complexes in the developing nervous system
  JOURNAL   Mol Cell Neurosci 18 (3), 307-319 (2001)
   PUBMED   11591131
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK138434.1, AK004975.1 and AL603711.6.
            
            On Mar 24, 2006 this sequence version replaced NM_027915.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.11966.1, AK004975.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1256              AK138434.1         2-1257
            1257-3346           AK004975.1         1181-3270
            3347-5369           AL603711.6         1181-3203           c
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 50.3 cM"
     gene            1..5369
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /note="adaptor-related protein complex 2, beta 1 subunit"
                     /db_xref="GeneID:71770"
                     /db_xref="MGI:MGI:1919020"
     exon            1..141
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            142..201
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..2978
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /note="isoform b is encoded by transcript variant 2; AP-2
                     complex subunit beta; beta-adaptin; beta2-adaptin; adaptor
                     protein complex AP-2 subunit beta; adapter-related protein
                     complex 2 beta subunit; clathrin assembly protein complex
                     2 beta large chain; plasma membrane adaptor HA2/AP2
                     adaptin beta subunit; beta-2-adaptin; adapter-related
                     protein complex 2 subunit beta; adaptor-related protein
                     complex 2 subunit beta"
                     /codon_start=1
                     /product="AP-2 complex subunit beta isoform b"
                     /protein_id="NP_082191.1"
                     /db_xref="CCDS:CCDS25162.1"
                     /db_xref="GeneID:71770"
                     /db_xref="MGI:MGI:1919020"
                     /translation="MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVG
                     KDVSSLFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLI
                     RALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQMVEDQG
                     FLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTALNECTEWG
                     QIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDY
                     YNMLLKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYV
                     KLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCV
                     STLLDLIQTKVNYVVQEAIVVIRDIFRKYPNKYESIIATLCENLDSLDEPDARAAMIW
                     IVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVKLFLKKPSETQELVQQVLS
                     LATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLLDELICH
                     IGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTTTNLEQPQVIPSQ
                     GDLLGDLLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLVGQSFIPSSVPATFAPSPT
                     PAVVSSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEM
                     NFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKME
                     PLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLATWKDIPNENELQFQIKE
                     CHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTL
                     SLKCRAPEVSQYIYQVYDSILKN"
     exon            202..307
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            308..443
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            444..689
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            690..880
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1102
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1223
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1319
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1435
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1601
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1700
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1960
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2153
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2154..2300
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2446
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2576
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2577..2661
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2748
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2903
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     exon            2904..5369
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5349..5354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /note="hexamer: AATAAA"
     polyA_site      5369
                     /gene="Ap2b1"
                     /gene_synonym="1300012O03Rik; AP105B"
                     /note="major polyA site"
ORIGIN      
        1 atctttgtcc ctggcaaatt gggttttgcg cagtggctta gatctagaga gaatcgtgac
       61 gggcaggaaa ccattacacc accaccccgg ctgtgctctc cgactgccgc cgccgccgcc
      121 gccaccccgc ccttgcctcc ggttcacgct aaagatccag aatcatgact gactccaagt
      181 acttcacaac caataagaag ggagaaattt ttgaattaaa agctgaactc aacaatgaaa
      241 agaaagaaaa gaggaaggag gctgtgaaga aagtgattgc tgctatgacc gtggggaagg
      301 acgtgagctc tctcttccca gatgtggtga actgcatgca gactgacaac ctggaactaa
      361 agaagctcgt gtacctctat ctgatgaact atgccaagag tcagccagac atggccatca
      421 tggctgtcaa cagctttgtg aaggattgtg aagatcccaa tcctttgatt cgagccttgg
      481 cagttagaac catgggatgc atccgggtgg acaagattac agagtatctc tgtgaacccc
      541 tccgcaagtg cttgaaggat gaagacccct atgttcggaa aacggcagca gtatgcgtgg
      601 caaaactcca tgatatcaat gcccagatgg tggaagatca gggatttctg gattctctgc
      661 gggatctcat agcagattca aacccaatgg tggtggctaa tgctgtagca gcattgtctg
      721 agatcagtga gtctcaccca aacagcaact tacttgatct gaaccctcag aatatcaata
      781 agctgctcac agccctgaat gaatgcactg aatggggcca gattttcatc ctggactgcc
      841 tgtctaatta caaccctaaa gatgaccggg aagctcagag catctgtgag cgagtaacgc
      901 ctcggctctc ccacgccaac tctgcagtgg tgctttcagc cgtaaaagtc ctaatgaagt
      961 ttctagaatt gttgcccaag gactctgact actacaatat gctgctaaag aagttagcac
     1021 ctccacttgt cacgttgctg tctggggagc cagaagtaca gtacgtcgcc ctgaggaaca
     1081 tcaacctaat tgtccagaaa aggcctgaaa tcttgaagca ggaaatcaag gtcttctttg
     1141 tgaagtacaa tgatcctatc tatgttaaac tagagaagtt agacatcatg attcgtcttg
     1201 catcccaagc caacattgct caggttctgg cggagctgaa ggaatatgcc actgaagttg
     1261 atgtggactt tgttcgcaaa gctgtgaggg ccattggacg gtgtgccatc aaagtggagc
     1321 aatcagcaga acgctgtgta agcacactgc ttgatctaat ccagaccaaa gtaaattatg
     1381 tggtccaaga ggcaattgtt gtcatcaggg acatcttccg aaaatacccc aacaagtatg
     1441 aaagtattat tgccaccctc tgtgaaaact tggactccct ggatgaaccc gatgcccgag
     1501 cagctatgat ttggattgta ggagaatatg ctgaaagaat cgataacgca gatgaattac
     1561 tagagagttt cctggaaggt tttcatgacg aaagcaccca ggtgcagctc acactgctca
     1621 ccgccatagt gaagctgttt ctcaagaagc catcagaaac acaggagctg gtccaacagg
     1681 tcttgagctt agccacacag gactccgata accctgacct tcgagatcgg ggttatatat
     1741 attggcgcct tctttcaact gaccctgtga cagccaaaga agtggtgttg tctgagaagc
     1801 cattgatctc tgaggagaca gacctcattg agcctaccct cctggatgag ctcatctgcc
     1861 acattggttc tttggcctct gtgtaccaca aacctccaaa tgcttttgtg gaagggagtc
     1921 atgggattca tcgcaaacac ttgccaattc atcatgggag cactgatgca ggtgatagcc
     1981 ccgttggcac caccactaca accaacctgg agcagcctca ggtcattccc tctcaaggtg
     2041 accttctggg ggatcttttg aatcttgacc tgggtccccc agtgaatgtc ccgcaagtgt
     2101 cctccatgca gatgggagcg gtggatcttt taggaggagg actggatagc ctggtaggac
     2161 agtccttcat cccgtcatca gtgcctgcga ccttcgctcc ttcacctact cctgctgtgg
     2221 tcagcagtgg tctgaatgac ctgtttgagc tttccactgg gataggcatg gcacctggcg
     2281 gatatgtggc tcctaaggcg gtctggctac ctgcagtaaa ggctaaaggc ttggagattt
     2341 cggggacgtt tactcaccgc caagggcata tctatatgga aatgaacttc accaacaaag
     2401 ctctgcagca tatgacagac tttgccatcc agtttaacaa gaatagcttc ggtgtcatcc
     2461 caagcactcc cttggccata catactccac tgatgccaaa ccagagcatt gacgtctctc
     2521 tgccgctcaa caccttgggc ccagtcatga agatggagcc cctgaataac ctgcaggtgg
     2581 ctgttaaaaa caatattgat gtcttctact tcagctgcct catcccactc aatgtgcttt
     2641 ttgtagaaga tggcaaaatg gagcgccagg tcttccttgc aacgtggaag gatattccca
     2701 atgaaaatga actccagttt cagattaagg aatgtcattt aaatgctgac acagtttcca
     2761 gcaagttgca aaacaacaat gtttacacta ttgccaagag gaacgtggaa gggcaggaca
     2821 tgctgtacca gtccctgaag ctcactaatg gcatttggat tttggcagag ctgcggatcc
     2881 agccaggaaa ccccaattat acgctgtcgc tgaagtgtag agcccctgaa gtctctcagt
     2941 acatctatca ggtctacgac agcattttga aaaactaata aatgggtcca gtcagcctgt
     3001 aatcggtgca agccaagaac tcttaactgg aagacgttgt actgttgtgt agagcctgaa
     3061 cccaaaccct gcggtaccca ccccggtagt ggccagtcat cttgtgctga cattagcatt
     3121 caccctgttg gataggttag cttcccgaga catctccttc caccattccc cacttctgcc
     3181 acctgctgct cttctctgtc cttagttgtg agcttctcca tgctgtgcca atggctagcc
     3241 ttttctacac cctcttttga gtgtagtttg atattttgta atcaaaagct catttcacaa
     3301 gcagaaaaag gcgacaagtt aattaaagca aggaaaagtg tcactgaaac ataaactgca
     3361 ccttattgtt ttatattttt gtacagatga gaagttgagg tagaacactg agtagaaagg
     3421 gcgactgacc ctcatcaaac acagtcttag cggagatcca ctgatggccc tggcccctgc
     3481 tggggcgggg caggaggcag ccctgctcct tgccttttgc attgtcattc ctcccctctg
     3541 tccaggtcat tccttggcta agccttcgag ctcccttata cccatgctat ctggtttgac
     3601 aattccatat atggcagtgg tggtcttcac tgatggttgc gagtgcaggg acactggaca
     3661 tatgggctca gtgctgagag gtggcagagc tgcgctcctg cccacagtgc tagcccagga
     3721 atcctgggga ggctgagtag acatgagtaa gaacagggcg ccgccccgcc ccttcctccc
     3781 ccacccccct tccctgtgtt tttcagggct cattcaaagt taaaatcaga gtctcagagt
     3841 taaaacagac attaatgact tatccaaccc tgtttttcta aaaagaaagg taacaggcaa
     3901 aaagtgtgtt gccgaggcct cttggctctt gagtagcaga gctgagactg gttccctgat
     3961 gctccgtcag cttccctatg tggctggctc attattttcc tcaaagcctt ctgtctttcc
     4021 tgtgcctgcc tgtgactctt tgcacactct gtactgagtt tggtgctcca cccattcact
     4081 ggggtcaggt ggcagttgac atcttggtta taagtatgca tgcaggtaac aagagtgaaa
     4141 gcagagctga catttgccag gctttcctgg agcaggccag tgagtgtaga cgtgtgtaaa
     4201 gatgtcgcca gttctcactg tctgtcagct cactgagctg cccttcatcc atggtccttg
     4261 agctccagag cggagggaga ttctctcagc aggcccatct cacaaagact ggggatgtga
     4321 acacattggt gtgagtcatc agaaaccccg tactcatggc cctgctcagg gctgctgttg
     4381 actctgcttg agaggtggca cttgtcgaac agcctggcac ctggtatcca ggctccaacg
     4441 acctcatgca ggactcagac ctgacccact gaatcccgag gagaggctga aaaaatcaga
     4501 gtagaaacca gcaacattta caagtgtgtc tggagtaatc ctgtttcctc ccagctgcaa
     4561 agcttctctc aagttcctta atccttgaga atcttaagag gcttgctggc tggtgaaaaa
     4621 gaaatacatc cctccctcca tcaccatgtg gggatgcagg aaatcctgca ggggttgtgt
     4681 gggaggcaga tagatggtgg tatgttgggt ggaaagacaa gttgaccaag ttcccttgtc
     4741 gatgctgatt cctggagtca gttggttatg agtagtataa gggattccca ctacaaatgc
     4801 agtgttccca tctcaaagca aaactgtagt tcccaaaaga agatgtgtca gggcaagcag
     4861 aaagggaagt ttaggccaga atgtggccat cccagaaact gaggcctctc tcagcctcac
     4921 cagtgacaga gaacagctgt ggttctctgc tggcctcagc tgatccatgc tgtctgactc
     4981 ccggggagag ttaggaagat gcaaggacct ctcctcaaga gactgttgcc tgcctgaagc
     5041 ttctgttcag gctcatgcca agcttgaccg gattgggaga actggactca gtttaacctg
     5101 tatccactct ccttagccaa agtgtgcgtc tgtcgaaagc tgaagtgcag agggcaggga
     5161 agcaaacaca ccagagctct ttctggtcag attccccagg tgtttgtccc cagccaagcc
     5221 tggtccgtat tgtgaagctt gggatgctgg agccaatgct gtcccaagaa ggcttacttt
     5281 gctgttcttg ttgggatctg ttgttgggga gggtggggtt tggggagcct ttaccttgtg
     5341 tactcatcaa taaactcatt tacacaaaa
//
