LOCUS       NM_007459               4639 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus adaptor-related protein complex 2, alpha 2 subunit
            (Ap2a2), transcript variant 2, mRNA.
ACCESSION   NM_007459
VERSION     NM_007459.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Xu,Z., Qiao,S., Qian,W., Zhu,Y., Yan,W., Shen,S. and Wang,T.
  TITLE     Card9 protects fungal peritonitis through regulating Malt1-mediated
            activation of autophagy in macrophage
  JOURNAL   Int Immunopharmacol 110, 108941 (2022)
   PUBMED   35850054
REFERENCE   3  (bases 1 to 4639)
  AUTHORS   Lopez-Hernandez,T., Takenaka,K.I., Mori,Y., Kongpracha,P.,
            Nagamori,S., Haucke,V. and Takamori,S.
  TITLE     Clathrin-independent endocytic retrieval of SV proteins mediated by
            the clathrin adaptor AP-2 at mammalian central synapses
  JOURNAL   Elife 11, e71198 (2022)
   PUBMED   35014951
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4639)
  AUTHORS   Montgomery,M.K., Bayliss,J., Keenan,S., Rhost,S., Ting,S.B. and
            Watt,M.J.
  TITLE     The role of Ap2a2 in PPARalpha-mediated regulation of lipolysis in
            adipose tissue
  JOURNAL   FASEB J 33 (12), 13267-13279 (2019)
   PUBMED   31533003
REFERENCE   5  (bases 1 to 4639)
  AUTHORS   Azarnia Tehran,D., Lopez-Hernandez,T. and Maritzen,T.
  TITLE     Endocytic Adaptor Proteins in Health and Disease: Lessons from
            Model Organisms and Human Mutations
  JOURNAL   Cells 8 (11), 1345 (2019)
   PUBMED   31671891
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4639)
  AUTHORS   Owen,D.J., Vallis,Y., Noble,M.E., Hunter,J.B., Dafforn,T.R.,
            Evans,P.R. and McMahon,H.T.
  TITLE     A structural explanation for the binding of multiple ligands by the
            alpha-adaptin appendage domain
  JOURNAL   Cell 97 (6), 805-815 (1999)
   PUBMED   10380931
REFERENCE   7  (bases 1 to 4639)
  AUTHORS   Odorizzi,G., Cowles,C.R. and Emr,S.D.
  TITLE     The AP-3 complex: a coat of many colours
  JOURNAL   Trends Cell Biol 8 (7), 282-288 (1998)
   PUBMED   9714600
  REMARK    Review article
REFERENCE   8  (bases 1 to 4639)
  AUTHORS   Jurecic,R., Nachtman,R.G., Colicos,S.M. and Belmont,J.W.
  TITLE     Identification and cloning of differentially expressed genes by
            long-distance differential display
  JOURNAL   Anal Biochem 259 (2), 235-244 (1998)
   PUBMED   9618202
REFERENCE   9  (bases 1 to 4639)
  AUTHORS   Newman,L.S., McKeever,M.O., Okano,H.J. and Darnell,R.B.
  TITLE     Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific
            vesicle coat protein
  JOURNAL   Cell 82 (5), 773-783 (1995)
   PUBMED   7671305
REFERENCE   10 (bases 1 to 4639)
  AUTHORS   Robinson,M.S.
  TITLE     Cloning of cDNAs encoding two related 100-kD coated vesicle
            proteins (alpha-adaptins)
  JOURNAL   J Cell Biol 108 (3), 833-842 (1989)
   PUBMED   2564002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY729047.1, AK173054.1 and BC058099.1.
            
            On Dec 20, 2007 this sequence version replaced NM_007459.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058099.1, AK128972.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                BY729047.1         1-63
            64-3277             AK173054.1         1-3214
            3278-4639           BC058099.1         3220-4581
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 86.94 cM"
     gene            1..4639
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="adaptor-related protein complex 2, alpha 2 subunit"
                     /db_xref="GeneID:11772"
                     /db_xref="MGI:MGI:101920"
     exon            1..247
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..2997
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="isoform 2 is encoded by transcript variant 2; AP-2
                     complex subunit alpha-2; alpha2-adaptin; 100 kDa coated
                     vesicle protein C; adaptor protein complex AP-2 subunit
                     alpha-2; adapter-related protein complex 2 alpha-2
                     subunit; clathrin assembly protein complex 2 alpha-C large
                     chain; plasma membrane adaptor HA2/AP2 adaptin alpha C
                     subunit; adaptor-related protein complex AP-2, alpha 2
                     subunit; alpha-C adaptin; alpha-adaptin C; adaptor protein
                     complex AP-2, alpha 2 subunit; adapter-related protein
                     complex 2 subunit alpha-2; adaptor-related protein complex
                     2 subunit alpha-2"
                     /codon_start=1
                     /product="AP-2 complex subunit alpha-2 isoform 2"
                     /protein_id="NP_031485.3"
                     /db_xref="CCDS:CCDS40188.1"
                     /db_xref="GeneID:11772"
                     /db_xref="MGI:MGI:101920"
                     /translation="MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKF
                     KGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLV
                     NSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDT
                     MDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKN
                     PEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVR
                     GRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLG
                     QFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLL
                     YAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIR
                     IAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGN
                     LIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDS
                     QLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTV
                     TDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGL
                     LVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIF
                     YGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEA
                     PVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKA
                     KHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQM
                     YRLTLRTSKDTVSQRLCELLSEQF"
     exon            248..316
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            317..459
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            460..653
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            654..783
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            784..885
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            886..994
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1142
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1311
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1449
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1632
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1730
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1962
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2136
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2300
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2383
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2473
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2597
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2714
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2784
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2919
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..4639
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3255..3260
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="hexamer: ATTAAA"
     polyA_site      3284
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="major polyA site"
     regulatory      3307..3312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="hexamer: ATTAAA"
     polyA_site      3339
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
     regulatory      4613..4618
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="hexamer: AATAAA"
     polyA_site      4639
                     /gene="Ap2a2"
                     /gene_synonym="2410074K14Rik; Adtab; L25"
                     /note="major polyA site"
ORIGIN      
        1 gaggctcagc gtgcgcgcca gcgcgtgcgt cgtgcgtgct cgcgcgcgca gcccagcgag
       61 cggcgctcgg gggcccgagg cggctgtggc taggcggctc cgcgctccag ggctcttcgg
      121 cgtgaccgcc gcgcccgtgc tgttctagcc cgccgccgag gccgctcccg gggcgcaaag
      181 atgccggccg tatccaaggg ggacgggatg cgaggcctag cggtcttcat ctccgacatc
      241 cgcaactgta aaagtaaaga agctgaaata aagagaataa acaaagaact ggcaaatatt
      301 agatccaaat ttaaaggtga caaggctctt gatggctaca gtaaaaagaa gtatgtctgc
      361 aaattgctct tcatctttct ccttggtcat gacattgact ttggacacat ggaagctgtg
      421 aatcttctga gctcaaacag atacacggaa aagcagattg gctacctttt catctctgta
      481 ttggtgaact cgaatagtga actgatccgg ttgattaaca atgccatcaa gaatgacctg
      541 gccagccgca atcctacgtt tatgggtctc gccctgcact gcattgccaa tgtgggtagc
      601 cgtgagatgg cggaggcctt cgccggagag atccccaaga tcctcgtggc aggagatacc
      661 atggatagtg tgaagcagag cgcagccctg tgcttgttac gcctgtacag gacatcacct
      721 gacctagttc ccatgggcga ctggacatct cgtgtggtac acctcctcaa tgaccagcac
      781 ttgggggtag tgactgcagc gactagcctc atcaccaccc tggcccagaa gaaccctgag
      841 gagttcaaga cctctgtctc gctagctgtc tcgcgactga gcaggattgt gacgtctgca
      901 tcaacagatc ttcaggatta tacttactac tttgtcccgg ctccttggct gtcagtcaag
      961 cttctgagac tgctgcagtg ctacccaccc ccagaccctg cggtgcgtgg ccgcctgact
     1021 gagtgcttgg agaccatcct gaacaaggcc caggagccac ccaagtccaa gaaggtccag
     1081 cattcaaatg ccaagaacgc tgtgctgttt gaggccatca gcctgatcat ccaccatgac
     1141 agtgagccaa acttgctcgt ccgcgcctgc aaccagctgg gccagttcct gcagcaccgg
     1201 gagacgaacc tacgctacct ggctctggag agcatgtgca cgctggccag ctctgagttc
     1261 tcccacgagg ccgtcaagac ccacattgag acggtcatca atgccttgaa gacggaaaga
     1321 gacgtgagtg tgaggcagcg ggcagtggac ctgctctacg ccatgtgtga ccggagcaac
     1381 gcccagcaga ttgtggctga gatgctgagc tacctagaaa cggctgacta ctccatccga
     1441 gaggaaattg tgctgaaggt ggccatcctg gctgagaagt atgcagtgga ctacacttgg
     1501 tatgtggaca ccatcctcaa cctcatccgc atcgcgggcg actatgtgag cgaggaggtg
     1561 tggtaccgcg tcatccagat tgtcatcaat cgcgatgatg tgcagggcta tgctgccaag
     1621 acggtatttg aggcattgca ggcgccagca tgtcatgaga acttggtcaa agtgggcggc
     1681 tacatcctgg gggagtttgg aaacttgata gctggggacc cacgatccag ccctctgatc
     1741 cagttcaacc tgctccactc caagttccac ttgtgcagcg tccccactcg ggcactcctg
     1801 ctgtccacct acatcaagtt cgtgaacctc tttccagagg taaaagctac cattcaggac
     1861 gtgctgcgca gtgacagcca gctgaagaat gccgacgtgg agctgcagca gcgggctgtg
     1921 gagtacctgc gactgagcac tgtcgccagc actgacatcc tggcaactgt cctggaggaa
     1981 atgccaccct tccctgagcg tgagtcctcc atcttggcca agctgaagaa gaagaagggc
     2041 ccaagcacag tgactgacct ggaggagacc aagcgggagc gaagcatcga tgtgaatggg
     2101 ggccctgaac ccgttccagc cagcaccagt gctgcgtcca caccttctcc gtcagcagac
     2161 ctgctgggtc tgggggccgt gccccctgct cccacggggc cccctccctc ctctggcggc
     2221 gggctgctgg tggacgtgtt ctcagactca gcttctgcag tcgcacctct tgcacccggc
     2281 tctgaagaca actttgccag gtttgtttgt aaaaataatg gtgtattgtt cgaaaaccag
     2341 ctgcttcaaa ttggacttaa gtcagaattt cggcagaatt taggccgaat gttcatattt
     2401 tatggtaaca agacctccac gcagttccta aacttcactc caacactaat ctgtgctgac
     2461 gaccttcaaa ctaatctgaa cctgcagact aagcccgtgg acccaactgt ggatgggggc
     2521 gcacaggtgc aacaagtggt caacattgag tgtatatctg atttcacaga ggcacctgtc
     2581 ctcaacatcc agttcaggta tggtgggacc ttccagaatg tgtctgttaa gcttcccatc
     2641 actctcaaca aatttttcca gcccacagaa atggcttctc aggatttctt tcaacgttgg
     2701 aagcagttga gcaatccaca gcaagaagtg cagaatatct tcaaagcaaa gcatccgatg
     2761 gacacagaga tcactaaggc aaagattatt ggatttggtt ctgcgctcct ggaagaagtt
     2821 gacccgaatc ctgcaaattt cgtgggtgct ggcataatac acaccaaaac cacccagatt
     2881 ggctgcttgt tgcgcctgga gccaaacctg caagctcaga tgtatagact caccttgcgt
     2941 accagcaaag acaccgtctc tcagagacta tgtgaattgc tgtcggaaca gttctagtcc
     3001 gcagaccaga agatcaggct ctttgtttgt gcgtctctct tgtctctctc gtttgtcttt
     3061 gtaccacact gcagataaac accccttgtc tgaaaactgc cattcacggc acctccaggc
     3121 ctatgcccac agccagcccc tgggtgtgga gggaatgcag gactcagttt gagaagggct
     3181 gcagcaggcc tgccccgtca aagaagggtg cctgagcctg ggaaatcagg gcagtcctgt
     3241 ggttcaacct acaaattaaa gggaaattag aagtttgaac gaaagtggaa attttgttca
     3301 aattttatta aaatgccact ggctcaatct cagaatccag aaacagcgcc atgtgctttt
     3361 agggttaacc cagggctgcg tggctctgct ggatggtgac agcggtggct tagaggtatg
     3421 tgacctcatg ggctaggcag tgtccatgga gtttgctgga gcactgcctg gtgtataact
     3481 gtttgaaact ccatgcccca cgcatggcag ggtcccgtgc ctaacaagcc cagtgttctt
     3541 ggagggacag ttgaaacatt ttaccacttt tttgagatct taggtttgca gcatggtggg
     3601 caattcctct tgactttgga agacatggat atcacccatc tttgttttta atgaatgtcc
     3661 tatccctatg gtacattctg gggacctgaa agctaagtgg gcgtggggtg gtaaggtaat
     3721 gactgtgaga catgcttctc tccagagggt cttgattggt gcccagtgtt acatcctcac
     3781 ttctgtgaag cccttgtggc tgggtggaaa agagagacct aagtgtggca gtggagtgac
     3841 agtctggggc gagaatggca acccaggcag gtgctgcgct aaggatgcac tggactccct
     3901 agctcatggg ccatttaacc ctttgtgggc attggactgt atgtacggcc catcagggga
     3961 agaacatagg cagcgtcagg agctcaggct tggggccttg cactatgtct tgcagtaatg
     4021 cccaggattg ctatagactg agctgtatgg ggtcatctgt acgtacacag cagtgttgtg
     4081 tcccttgccc actggagcac taggtgtggt cagttatgca cgggtctttt tttttttttt
     4141 ttttgcctca gcctcaggct gccagcctgc catgtgggtt tgaggactgc agcagggctc
     4201 taactactca agctcccgca gccccactct gtatagccca ggcaggtagt ttccgaacac
     4261 caccacctgt gtgctgatcc ttgcgttcca cgaagccctt ggtctgccta tcgtgttgtg
     4321 acccacactg ccatggcaca ggcttaggtt ccagatgatc tagctgtggc cacaatgagc
     4381 ctccgaatcc ccttgctccg tagctgtcca ttgtgtgcag tccacgcagt attgcatgga
     4441 ccacgtgggt agctcaacct gtgtggctgt ggcttgtgta ccacgtgctg cccagtgatg
     4501 gtgtctgtat cagtaatgtc ccagcccacg ggagctgctg gggcacaggc ccactctccg
     4561 tgactaccgg gcctccatta gtccagtgtg cttgttctca gaggaaaaaa aaaataaatg
     4621 ctaggacagg aaactggaa
//
