LOCUS       NM_007458               3457 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus adaptor-related protein complex 2, alpha 1 subunit
            (Ap2a1), transcript variant 1, mRNA.
ACCESSION   NM_007458 XM_001002959 XM_001002965 XM_001002976 XM_001002983
VERSION     NM_007458.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Camblor-Perujo,S., Ozer Yildiz,E., Kupper,H., Overhoff,M.,
            Rastogi,S., Bazzi,H. and Kononenko,N.L.
  TITLE     The AP-2 complex interacts with gamma-TuRC and regulates the
            proliferative capacity of neural progenitors
  JOURNAL   Life Sci Alliance 7 (2), e202302029 (2023)
   PUBMED   38086550
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3457)
  AUTHORS   Lopez-Hernandez,T., Takenaka,K.I., Mori,Y., Kongpracha,P.,
            Nagamori,S., Haucke,V. and Takamori,S.
  TITLE     Clathrin-independent endocytic retrieval of SV proteins mediated by
            the clathrin adaptor AP-2 at mammalian central synapses
  JOURNAL   Elife 11, e71198 (2022)
   PUBMED   35014951
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3457)
  AUTHORS   Fukaya,M., Sugawara,T., Hara,Y., Itakura,M., Watanabe,M. and
            Sakagami,H.
  TITLE     BRAG2a Mediates mGluR-Dependent AMPA Receptor Internalization at
            Excitatory Postsynapses through the Interaction with PSD-95 and
            Endophilin 3
  JOURNAL   J Neurosci 40 (22), 4277-4296 (2020)
   PUBMED   32341099
REFERENCE   5  (bases 1 to 3457)
  AUTHORS   Azarnia Tehran,D., Lopez-Hernandez,T. and Maritzen,T.
  TITLE     Endocytic Adaptor Proteins in Health and Disease: Lessons from
            Model Organisms and Human Mutations
  JOURNAL   Cells 8 (11), 1345 (2019)
   PUBMED   31671891
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3457)
  AUTHORS   Odorizzi,G., Cowles,C.R. and Emr,S.D.
  TITLE     The AP-3 complex: a coat of many colours
  JOURNAL   Trends Cell Biol 8 (7), 282-288 (1998)
   PUBMED   9714600
  REMARK    Review article
REFERENCE   7  (bases 1 to 3457)
  AUTHORS   Newman,L.S., McKeever,M.O., Okano,H.J. and Darnell,R.B.
  TITLE     Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific
            vesicle coat protein
  JOURNAL   Cell 82 (5), 773-783 (1995)
   PUBMED   7671305
REFERENCE   8  (bases 1 to 3457)
  AUTHORS   Ball,C.L., Hunt,S.P. and Robinson,M.S.
  TITLE     Expression and localization of alpha-adaptin isoforms
  JOURNAL   J Cell Sci 108 (Pt 8), 2865-2875 (1995)
   PUBMED   7593326
REFERENCE   9  (bases 1 to 3457)
  AUTHORS   Ko,M.S., Wang,X., Horton,J.H., Hagen,M.D., Takahashi,N., Maezaki,Y.
            and Nadeau,J.H.
  TITLE     Genetic mapping of 40 cDNA clones on the mouse genome by PCR
  JOURNAL   Mamm Genome 5 (6), 349-355 (1994)
   PUBMED   8043949
REFERENCE   10 (bases 1 to 3457)
  AUTHORS   Robinson,M.S.
  TITLE     Cloning of cDNAs encoding two related 100-kD coated vesicle
            proteins (alpha-adaptins)
  JOURNAL   J Cell Biol 108 (3), 833-842 (1989)
   PUBMED   2564002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CA327188.1 and BC031433.1.
            
            On Oct 18, 2006 this sequence version replaced NM_007458.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031433.1, SRR1660811.104228.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                CA327188.1         7-56
            51-3457             BC031433.1         1-3407
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.01 cM"
     gene            1..3457
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /note="adaptor-related protein complex 2, alpha 1 subunit"
                     /db_xref="GeneID:11771"
                     /db_xref="MGI:MGI:101921"
     exon            1..211
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..3078
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /note="isoform a is encoded by transcript variant 1; AP-2
                     complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A;
                     100 kDa coated vesicle protein A; adaptor protein complex
                     AP-2 subunit alpha-1; adapter-related protein complex 2
                     alpha-1 subunit; clathrin assembly protein complex 2
                     alpha-A large chain; adaptor protein complex AP-2, alpha 1
                     subunit; adapter-related protein complex 2 subunit
                     alpha-1; adaptor-related protein complex 2 subunit
                     alpha-1; plasma membrane adaptor HA2/AP2 adaptin alpha A
                     subunit; adaptor-related protein complex AP-2, alpha 1
                     subunit"
                     /codon_start=1
                     /product="AP-2 complex subunit alpha-1 isoform a"
                     /protein_id="NP_031484.1"
                     /db_xref="CCDS:CCDS52239.1"
                     /db_xref="GeneID:11771"
                     /db_xref="MGI:MGI:101921"
                     /translation="MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKF
                     KGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLV
                     NSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDS
                     MDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKN
                     PDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAV
                     KGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQL
                     GQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADL
                     LYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLI
                     RIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFG
                     NLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAG
                     SQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGA
                     ASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLV
                     DVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEA
                     DELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHP
                     GDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLK
                     LPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSA
                     LLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCEL
                     LAQQF"
     exon            212..280
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            281..423
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            424..617
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            618..747
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            748..849
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            850..958
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1109
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1278
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1416
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1599
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1697
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1929
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2097
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2258
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2324
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2381
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2464
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2554
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2678
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2795
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2796..2865
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..3000
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     exon            3001..3443
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3414..3419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /note="hexamer: AATAAA"
     polyA_site      3443
                     /gene="Ap2a1"
                     /gene_synonym="Adtaa"
                     /note="major polyA site"
ORIGIN      
        1 cggggcgtcg gcctgcagcc agcccgccgc tagcccgccc tcccccggcc ggctcctcgg
       61 cgccgtctgg ggtcctttcc gcccggtgcc gccagccagc cccgcgcccg ttcggccagg
      121 cttggagccg agccctccgc catcatgccg gctgtatcca aaggcgatgg catgcgtggg
      181 ctcgccgtgt tcatctccga catccggaac tgcaagagca aagaggctga gatcaagagg
      241 atcaacaagg aactggccaa catccgttcc aagttcaaag gggacaaggc cttggatggc
      301 tacagtaaaa agaagtatgt gtgtaagctg ctcttcatat tcctgcttgg ccatgacatt
      361 gactttggac atatggaggc cgtgaacctg ctaagctcta acaagtacac ggagaagcag
      421 atagggtacc tgttcatctc agtcctggtg aactcgaact cagaactgat ccggctcatc
      481 aacaacgcta tcaagaatga cctggccagt cgcaacccca ccttcatgtg cctggccttg
      541 cactgtatcg ctaacgtggg cagccgtgag atgggcgagg cttttgctgc agacattccc
      601 cgaatcctgg tggctgggga cagcatggac agtgtgaagc agagtgccgc cctatgccta
      661 ctgcgactct acaaggcctc gcccgacttg gtgcccatgg gcgagtggac ggcacgtgta
      721 gtgcacttgc tcaatgatca gcacatggga gtggtcacag ctgctgtcag cctcatcacc
      781 tgtctctgca agaagaatcc ggatgacttc aagacctgta tctccctggc tgtgtctcgc
      841 ttaagccgga tcgtctcctc agcctccact gacctccagg actacactta ctacttcgtt
      901 cctgcaccct ggctctctgt gaagctactg cggctgctcc agtgttaccc accaccagag
      961 gatgcagccg tgaaagggcg gttagtggag tgtctggaga ctgtgctcaa caaggcccag
     1021 gagcctccca agtccaagaa ggtgcagcac tccaacgcca agaacgctat cctctttgag
     1081 accattagcc tcatcatcca ctatgacagt gagcccaacc tcctggtccg cgcctgcaac
     1141 cagctgggcc agttcctgca gcaccgggag actaacctgc gctacctggc cctggagagc
     1201 atgtgcacgc tggccagctc cgagttctcc cacgaggccg tcaagaccca cattgataca
     1261 gttattaatg ccctcaagac ggagcgggac gtcagtgtga ggcagcgggc ggctgatctc
     1321 ctgtatgcca tgtgtgaccg gagcaatgcc aagcagattg tgtcagagat gctgcggtac
     1381 ctggagactg ctgactatgc catccgagag gagatcgtgc tgaaggtggc catcctggct
     1441 gagaagtatg cagtggacta cagctggtac gtggacacca tcctcaacct catccgcatc
     1501 gcgggcgact atgtgagcga ggaggtgtgg taccgcgtgc tgcagatcgt caccaaccgt
     1561 gatgacgtcc agggttatgc tgccaagaca gtgtttgagg ccctccaggc cccagcctgt
     1621 catgagaaca tggtgaaggt cggtggctac atccttgggg agtttgggaa cttgattgct
     1681 ggggacccac gctccagccc accagtgcag ttctcgctgc tgcactccaa gttccacctg
     1741 tgcagcgtgg ccacccgcgc tctgttgctg tccacctaca tcaagttcat caacctcttc
     1801 cctgagacca aggccaccat ccaaggggtt ctgcgtgccg gctcccagct gcgaaatgcc
     1861 gacgtggagc tacagcagcg ggccgtggag tacctcaccc tcagctccgt agccagcacc
     1921 gatgttctgg ctacggtgct agaagaaatg cccccatttc ccgagcggga gtcgtccatc
     1981 ttggccaagc tgaagcgcaa gaagggccct ggggcagcca gtgccctaga tgacagccgc
     2041 agggacacca gcagcaatga catcaatggg ggtgtggagc ccacccccag cactgtgtcg
     2101 accccctcac cctccgcgga cctcttaggg ctgcgggcag cccctccccc tgctgcaccc
     2161 ccggctcccg taggcgggaa cctcctggtg gatgtcttct ctgacggccc cactgcacag
     2221 cccagcctgg ggcccactcc tgaggaggcc ttcctcagcg agctggagcc ccctgcccct
     2281 gagagcccca tggctttgtt ggctgaccca gctccagctg ctgacccagg tcctgaggac
     2341 ataggccctc ccattccaga agcagatgaa ctgttgaata agttcgtgtg taagaatagt
     2401 ggggtcttgt ttgagaacca gctgctgcag attggagtca agtctgagtt ccggcagaac
     2461 ctgggccgca tgtatctctt ctatggcaac aagacttctg tgcagttcca gaacttcttg
     2521 cccaccgtgg tccatcctgg ggacctccag actcagctgg cggtgcagac caagcgtgtg
     2581 gcggcacaag tggacggtgg cgcacaggtg cagcaagtac tcaacattga gtgtctgcga
     2641 gacttcctga cgccgccact gttgtcggtg cgcttccggt acggtggcac cgcccagtcc
     2701 ctcactctga agctcccagt gaccatcaac aaattcttcc agcccacaga gatggcggcc
     2761 caagactttt tccagcgctg gaagcagctg agcctccccc tgcaggaggc acagaaaatc
     2821 ttcaaagcca accacccgat ggatgctgaa gttactaagg ccaagcttct ggggtttggc
     2881 tctgctcttc tggacaatgt ggatcccaac cctgagaact ttgtgggtgc tggaatcatc
     2941 cagacgaagg ccctgcaggt ggggtgtctg cttcggctgg agcccaatgc ccaggcccaa
     3001 atgtaccgtc taaccctgcg caccagcaaa gagcctgtct cccgtcactt gtgtgagctg
     3061 ctggcccagc agttctgagc cctggacttg ccctggtata tggccagtgc caggcagccc
     3121 tgggatggag gcagctgtgg tggagcggga cagcgagggg ccctccagtg gcgacagcaa
     3181 agagcccggg gtgggggcgc ctgggagctc cctctggcct tttgtatttt atttttgttc
     3241 atctgctgct gtttacattc tagggggggg tcaagggaag caccctccct cccttgtccc
     3301 ccgaagcaca gaggggagag gggccgagga agtaggtacc tctccccccc cttccctgtc
     3361 accctccccc ttcctccctt tccccatcca ggggctgtgt attattgtga gcgaataaac
     3421 agagagacgc taatattacc ccaaaaaaaa aaaaaaa
//
