LOCUS       NM_001290379            2226 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus adaptor-related protein complex 1, sigma 2 subunit
            (Ap1s2), transcript variant 3, mRNA.
ACCESSION   NM_001290379 XM_006528675 XM_006528676
VERSION     NM_001290379.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Sengul,G.F., Mishra,R., Candiello,E. and Schu,P.
  TITLE     Hsc70 phosphorylation patterns and calmodulin regulate AP2
            Clathrin-Coated-Vesicle life span for cell adhesion protein
            transport
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (1), 119611 (2024)
   PUBMED   37926156
REFERENCE   3  (bases 1 to 2226)
  AUTHORS   Mishra,R., Sengul,G.F., Candiello,E. and Schu,P.
  TITLE     Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1,
            Sgip1/AP2, synaptojanin1 interactome
  JOURNAL   Sci Rep 11 (1), 8007 (2021)
   PUBMED   33850201
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2226)
  AUTHORS   Nakano,Y., Wiechert,S. and Banfi,B.
  TITLE     Overlapping Activities of Two Neuronal Splicing Factors Switch the
            GABA Effect from Excitatory to Inhibitory by Regulating REST
  JOURNAL   Cell Rep 27 (3), 860-871 (2019)
   PUBMED   30995482
REFERENCE   5  (bases 1 to 2226)
  AUTHORS   Candiello,E., Mishra,R., Schmidt,B., Jahn,O. and Schu,P.
  TITLE     Differential regulation of synaptic AP-2/clathrin vesicle uncoating
            in synaptic plasticity
  JOURNAL   Sci Rep 7 (1), 15781 (2017)
   PUBMED   29150658
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2226)
  AUTHORS   Bultema,J.J. and Di Pietro,S.M.
  TITLE     Cell type-specific Rab32 and Rab38 cooperate with the ubiquitous
            lysosome biogenesis machinery to synthesize specialized
            lysosome-related organelles
  JOURNAL   Small GTPases 4 (1), 16-21 (2013)
   PUBMED   23247405
  REMARK    Review article
REFERENCE   7  (bases 1 to 2226)
  AUTHORS   Nakano,Y., Jahan,I., Bonde,G., Sun,X., Hildebrand,M.S.,
            Engelhardt,J.F., Smith,R.J., Cornell,R.A., Fritzsch,B. and Banfi,B.
  TITLE     A mutation in the Srrm4 gene causes alternative splicing defects
            and deafness in the Bronx waltzer mouse
  JOURNAL   PLoS Genet 8 (10), e1002966 (2012)
   PUBMED   23055939
REFERENCE   8  (bases 1 to 2226)
  AUTHORS   Glyvuk,N., Tsytsyura,Y., Geumann,C., D'Hooge,R., Huve,J.,
            Kratzke,M., Baltes,J., Boening,D., Klingauf,J. and Schu,P.
  TITLE     AP-1/sigma1B-adaptin mediates endosomal synaptic vesicle recycling,
            learning and memory
  JOURNAL   EMBO J 29 (8), 1318-1330 (2010)
   PUBMED   20203623
  REMARK    GeneRIF: Synaptic vesicle reformation in cultured neurons from
            adaptor protein complex AP-1 sigma-1B-deficient mice is reduced
            upon stimulation, and large endosomal intermediates accumulate.
            Erratum:[EMBO J. 2010 Jun 2;29(11):1941. Boning, Daniel [corrected
            to Boening, Daniel]]
REFERENCE   9  (bases 1 to 2226)
  AUTHORS   Heldwein,E.E., Macia,E., Wang,J., Yin,H.L., Kirchhausen,T. and
            Harrison,S.C.
  TITLE     Crystal structure of the clathrin adaptor protein 1 core
  JOURNAL   Proc Natl Acad Sci U S A 101 (39), 14108-14113 (2004)
   PUBMED   15377783
REFERENCE   10 (bases 1 to 2226)
  AUTHORS   Kirchhausen,T., Davis,A.C., Frucht,S., Greco,B.O., Payne,G.S. and
            Tubb,B.
  TITLE     AP17 and AP19, the mammalian small chains of the
            clathrin-associated protein complexes show homology to Yap17p,
            their putative homolog in yeast
  JOURNAL   J Biol Chem 266 (17), 11153-11157 (1991)
   PUBMED   2040623
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK045558.1.
            
            On or before Mar 12, 2014 this sequence version replaced
            XM_006528676.1, XM_006528675.1.
            
            Transcript Variant: This variant (3) lacks an exon and contains two
            alternate 3' terminal exons, resulting in a different 3' coding
            region and 3' UTR, compared to variant 1. The encoded isoform (3)
            is the same length as isoform 1 but has a distinct C-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045558.1, SRR1660815.281741.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2226              AK045558.1         2-2227
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 75.97 cM"
     gene            1..2226
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /note="adaptor-related protein complex 1, sigma 2 subunit"
                     /db_xref="GeneID:108012"
                     /db_xref="MGI:MGI:1889383"
     exon            1..257
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..740
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /note="isoform 3 is encoded by transcript variant 3; AP-1
                     complex subunit sigma-2; sigma 1B subunit of AP-1
                     clathrin; adaptor-related protein complex 1 subunit
                     sigma-1B; adaptor protein complex AP-1 sigma-1B subunit;
                     golgi adaptor HA1/AP1 adaptin sigma-1B subunit;
                     adapter-related protein complex 1 sigma-1B subunit;
                     clathrin assembly protein complex 1 sigma-1B small chain"
                     /codon_start=1
                     /product="AP-1 complex subunit sigma-2 isoform 3"
                     /protein_id="NP_001277308.1"
                     /db_xref="CCDS:CCDS72465.1"
                     /db_xref="GeneID:108012"
                     /db_xref="MGI:MGI:1889383"
                     /translation="MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPK
                     MCSFLEWRDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDI
                     IFNFEKAYFILDEFLLGGEVQETSKKNVLKAIEQADLLQEDAKEAETPRSVLEEIGLT
                     "
     exon            258..436
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..545
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     exon            546..683
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     exon            684..692
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     exon            693..2226
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1906..1911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /note="hexamer: AATAAA"
     polyA_site      1924
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /note="major polyA site"
     regulatory      2200..2205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
                     /note="hexamer: AATAAA"
     polyA_site      2221
                     /gene="Ap1s2"
                     /gene_synonym="1500012A13Rik; EST1"
ORIGIN      
        1 agcttggcgc gcggtctccg gtccgcctct ccgctcgccg cagcccgggc tcctcctccg
       61 tccagcctcc agggcgggat ctgtcggctc gccctaccct cgcagcctaa gcctgccctc
      121 caccagacgg gtcccctccc cgcggcacca cggcttccct tccacagcac ggcgacgggg
      181 gcttcccctc agcagctgcc ggcgcctcag tctccgcggc cagcccagct ccgccgcgtc
      241 cgcggcccgc cgccgccatg cagtttatgt tgctttttag tcgccaggga aagcttcgac
      301 tgcagaaatg gtatgtccca ctgtcagaca aagaaaagaa gaagatcaca agagaacttg
      361 ttcaaaccgt tttagcacgg aaacccaaga tgtgcagctt ccttgagtgg agagatctga
      421 agattgttta taaaagatat gccagtctat atttttgctg tgctattgag gatcaggaca
      481 atgaactgat taccctggaa ataatccatc gttacgtgga attacttgac aagtattttg
      541 gcagtgtatg tgaacttgat atcatcttta attttgagaa ggcctatttt attttggatg
      601 aatttctctt gggaggagaa gttcaggaaa catccaagaa aaatgtcctt aaagcaattg
      661 agcaggccga tctcctgcag gaggatgcaa aagaagctga aaccccacgt agtgttcttg
      721 aagaaattgg actgacataa ctgtcctccc ttgctgatga cttcttgtgg catttcacac
      781 tgtagatggt cactgcctct catgtccatg ttagctaatg gtgtaagatg atgtcttgtc
      841 agtattactg ttttgctaag ctgcttcatt caggcctacc caattttttt taaagggaac
      901 tttggttaat caaatgataa attaagggac ataaatatga attaggaaga tacagacatt
      961 ttaaagtata aaggccagtc tcataattat gtgcttatat aaaatatgtc tatatccatg
     1021 tattatccaa cagtttggca aaatacattt actattgtac ttaaaaaagg aaacttaaat
     1081 tatgtgaaac caaagactag agtaacattt cagtacacat aaaaaataaa aggaattgac
     1141 acatcacaca gatcaaatta atgcaagttt attgaaaatg tcagcgacca aattgatacc
     1201 aaatacccat attggataca atatacttta aatcaaaggg aatttagatg cttttagagg
     1261 actaacttag aatgatgctg ttttgcacta aaattcatta agtattctga atgaatggaa
     1321 caaggaacaa agactttcct aatcttgagc acataatttt taaggccaag taacatttga
     1381 ctccctgagg tgaagttttg gtggtcataa tcgtgttttc aatgccccaa aataaaatag
     1441 cacggttaat taccaggtat tcttcctaac agtatgttgc tctcttcacg aagtattaca
     1501 taaagtcatt atctaaagca tgaatgttcc ttccggggtg cttgttaaag ccaaatttat
     1561 tttgcttccc tattgcttcc ttccagttag aagtgctata gacttgacag cagctcccta
     1621 ttctttcctg tcactgtcta gtcgttggaa gtcttatgac agcttaaaag atacctttca
     1681 tatagcaaag tgagagttga ctgagcaaag tggcattggt tggccctata ttttttgaag
     1741 ctgtataaat gcgcggcatc acttttatac atcctgacac atacctgaac acatctccct
     1801 gatttctatg ttcccttgta atatgctagg ctggagtaag ctttgctgtg attgtgaaca
     1861 actttcaagc caccggaact gtcttatcac atcaccaatt aatgtaataa atgttctgca
     1921 acactgtggt gtgcttttct ctttccaaaa gttttgttca actagggaga tcttctaaag
     1981 gaagggtact cttttcttcc catacaatat tgacaattcc tctccatctg acaccccagc
     2041 cctgcagcga aatgtgatgg cgcttgcacg cacgcacgca cgcacgcacg cacacacgca
     2101 caaaaatagc agaaggatca aggaatgttc atgtatttca actcagtaaa aaaaaatcac
     2161 atagattcta tgctacttac ttacaacaca ttttcaatta ataaatgccg cttttaagcc
     2221 atgatg
//
