LOCUS       NM_001411484            2753 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus adaptor-related protein complex 1 associated
            regulatory protein (Ap1ar), transcript variant 3, mRNA.
ACCESSION   NM_001411484
VERSION     NM_001411484.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Mertins,P., Przybylski,D., Yosef,N., Qiao,J., Clauser,K.,
            Raychowdhury,R., Eisenhaure,T.M., Maritzen,T., Haucke,V., Satoh,T.,
            Akira,S., Carr,S.A., Regev,A., Hacohen,N. and Chevrier,N.
  TITLE     An Integrative Framework Reveals Signaling-to-Transcription Events
            in Toll-like Receptor Signaling
  JOURNAL   Cell Rep 19 (13), 2853-2866 (2017)
   PUBMED   28658630
REFERENCE   3  (bases 1 to 2753)
  AUTHORS   Schachtner,H., Weimershaus,M., Stache,V., Plewa,N., Legler,D.F.,
            Hopken,U.E. and Maritzen,T.
  TITLE     Loss of Gadkin Affects Dendritic Cell Migration In Vitro
  JOURNAL   PLoS One 10 (12), e0143883 (2015)
   PUBMED   26624014
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2753)
  AUTHORS   Hirst,J., Edgar,J.R., Borner,G.H., Li,S., Sahlender,D.A.,
            Antrobus,R. and Robinson,M.S.
  TITLE     Contributions of epsinR and gadkin to clathrin-mediated
            intracellular trafficking
  JOURNAL   Mol Biol Cell 26 (17), 3085-3103 (2015)
   PUBMED   26179914
REFERENCE   5  (bases 1 to 2753)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 2753)
  AUTHORS   Maritzen,T., Zech,T., Schmidt,M.R., Krause,E., Machesky,L.M. and
            Haucke,V.
  TITLE     Gadkin negatively regulates cell spreading and motility via
            sequestration of the actin-nucleating ARP2/3 complex
  JOURNAL   Proc Natl Acad Sci U S A 109 (26), 10382-10387 (2012)
   PUBMED   22689987
  REMARK    GeneRIF: Together with the observation that Gadkin-mediated
            inhibition of cell spreading requires its binding to ARP2/3, these
            data indicate that Gadkin is a negative regulator of ARP2/3
            function present on intracellular membranes
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163391.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422591.121373.1,
                                           SRR13422592.257287.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-349               AC163391.4         62772-63120
            350-454             AC163391.4         67101-67205
            455-503             AC163391.4         76956-77004
            504-530             AC163391.4         77659-77685
            531-556             AC163391.4         79484-79509
            557-653             AC163391.4         83263-83359
            654-752             AC163391.4         84575-84673
            753-808             AC163391.4         86974-87029
            809-882             AC163391.4         87662-87735
            883-1011            AC163391.4         87925-88053
            1012-2753           AC163391.4         91516-93257
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 56.54 cM"
     gene            1..2753
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /note="adaptor-related protein complex 1 associated
                     regulatory protein"
                     /db_xref="GeneID:211556"
                     /db_xref="MGI:MGI:2384822"
     exon            1..349
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     CDS             273..1274
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="AP-1 complex-associated regulatory protein
                     isoform 3"
                     /protein_id="NP_001398413.1"
                     /db_xref="GeneID:211556"
                     /db_xref="MGI:MGI:2384822"
                     /translation="MGNCCWTQCFGLLRREAGRLQRAGGGNLGKEENEDSHKKRGHIS
                     LSNKKMSDASCCTQCERSKYFRTCPRGEHLTIEFENLVESDEGESPGSSHRPLTEDEI
                     ADLQERHYDSIAEKQKDVDRKIQRQLALQEEKLRLEEEALYAAQREAARAARQRKLLE
                     QEKQRVAQHYHCSSGDYQSAGPEDDFESSLRNIKSQYEVFRSSRLSSDATVLTPNTES
                     SCDLMTKTKSTSGNDDSTSLDLEWEDEEGMNRMLPMRERSKTEEDILRAALKYSSKKG
                     SNPTSASDDSNGLEWENDFVSAEMDDNGNSEYSGFVNPVLELSDSAIKQSDTDQQIR"
     exon            350..454
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            455..503
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            504..530
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            531..556
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            557..653
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            654..752
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            753..808
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            809..882
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1011
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..2753
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2482..2487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /note="hexamer: AATATA"
     polyA_site      2501
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
     regulatory      2733..2738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /note="hexamer: AATAAA"
     polyA_site      2753
                     /gene="Ap1ar"
                     /gene_synonym="Gadkin"
                     /note="major polyA site"
ORIGIN      
        1 gtggcggcgc gggcctcccg cggctgccgg ggcggtgacg ggcgcgggcg gccgggcggg
       61 tggcgagcgg gccatgcgga cggctggaga cccgggaccc cacggccctg cgctcgtccc
      121 tcgcgccgcg gtagcccgga gcccgcgggc ctagcggcgg cctttgttcc cggcgtcgcc
      181 gtctgccgag ctccggccgg cgcggatgca gctgctccgc ctgggctcgc gctcggggca
      241 ggaggaggag cggcggctcc gggcggcatg cgatggggaa ctgctgctgg acgcaatgct
      301 tcgggctgct ccgccgggaa gcgggtcggc tgcagcgagc gggcggaggt aacttaggga
      361 aggaagaaaa tgaagactca cataagaaaa gaggtcacat ttcactctcc aacaagaaaa
      421 tgagtgatgc cagctgctgc acacaatgtg aaagatcaaa gtattttagg acatgcccaa
      481 gaggagagca cttgaccata gagtttgaga acctcgtgga gagtgatgag ggggagagcc
      541 caggaagcag ccataggcca ctcactgaag acgaaatcgc tgacttacag gagcggcatt
      601 atgattctat tgcagaaaag cagaaagatg tggataggaa aatccagagg cagttagcct
      661 tacaagagga gaagttaaga ctagaagagg aggctttata cgctgcccag cgtgaagcag
      721 ccagggcagc aaggcagcgg aagctcctgg agcaagaaaa gcagagagtt gctcagcact
      781 atcattgcag cagtggagac tatcaaagtg caggaccaga ggacgacttt gaatcttcct
      841 taagaaacat aaagtcacag tatgaagtgt ttcggagcag tagactctcc tctgacgcca
      901 cagtgctgac accaaacacc gagagcagct gtgacctgat gaccaaaacc aaatcaacga
      961 gtggaaatga cgacagcacc tccttagacc tagagtggga agatgaagaa ggaatgaaca
     1021 ggatgctgcc catgagggag cgctccaaga cagaggaaga catcctgcgg gcagcactga
     1081 agtacagcag caagaagggc agcaacccca cgtccgcctc ggatgactcc aacgggctgg
     1141 agtgggagaa cgactttgtc agtgccgaga tggacgacaa cggcaactct gagtactccg
     1201 ggtttgtgaa ccctgtgtta gaactgtcgg attctgccat caagcagtct gatacagacc
     1261 agcagatccg atagggtgaa accgtgacct tacttaccaa atgtttaaaa accaactaga
     1321 atgtacaggt ggcatgccaa ggctttttgt taaggcagca aaaaaccatg tgacaaatgc
     1381 ttgttttcct aggagcagga gtggagggac agctgagcgc ttcagtcagg tgtgatctta
     1441 acatctgcct tcttcgtccc aggactgact cagtgaatct gacatatcac acattcatgc
     1501 ctttctgctc acctcgtcaa gttctgcatc ttaagtctgt attggtttta ctctgattgc
     1561 tttatagaac taaatctgaa agtatttttt gttacttctc agcatatgtg caggcagcat
     1621 gaggagggat gggcgggttc cccgggaagg cagaccattc ccagcagcgt ctccctggtt
     1681 acttttacct ttcatgtcta cttggcggca gtaattggag atggcccaag cattgtgctg
     1741 atgtgcgggc ttccttacac acacacacac acacacacac acatgcacac atcctaagtc
     1801 ctgcttcact caatctgagc aagagatgac aggattaaag atgcttccaa agtttggata
     1861 ctttgagttg gtaataatta aaactaatac ataaaaatgc atatttaaaa tttttacttc
     1921 ctttcacatg ccattatatt ggctttgaat ttagatttta agttttcact ttctttttta
     1981 aaaattctat atttccagtg aatgatcttt gaaagatttt tacacagaac atattctgtg
     2041 aatgatttaa gaaaacacta aaatacgggt atttcaaagt acacacactc ctttctgatt
     2101 tgaaaggtcc cgttggctgt accgcccttt cctttgcctt gtagtgcaag gcgctctgtg
     2161 gctggaacaa attatgttag cgtattgacg acctgtgtct gagtcttaga gaaggccagg
     2221 gctgggaaat gtgtcctgac tgcagtcttt ggctgggctg aggactgact gtcactttgg
     2281 caagcacact gtattctgtc tcccttgctg ttcctctctc ttcggcagcg atggtgcatg
     2341 ctttcaacag tccaggacgg tccaggtaga aatagtagag ttcttgctgt ccgtgaaaac
     2401 ttatgtgggt ggccctttcg atatctgaac tgctatacaa tgacatctgt aattttgtca
     2461 tttttaaatc tcatacaaat aaatatactt tgatatgtaa atatgcatta ggttatgcta
     2521 ttatttatat ctatctcaag atttaaattg taggtgtgtc atggaaataa tttaaaggta
     2581 ctctatattc acattgcctg tgttaatgct ttttaaggtt tatattacat cagatgtata
     2641 tttttggttt ggcataagct actgttgtaa ttttcttggc tttttgttca taaagaattt
     2701 tctgaaggaa tggtaacttc tcaaggattg tgaataaaca tttttacatt taa
//
