LOCUS       NM_001277188            4177 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus anoctamin 4 (Ano4), transcript variant 1, mRNA.
ACCESSION   NM_001277188
VERSION     NM_001277188.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Russell,N.X., Burra,K., Shah,R.M., Bottasso-Arias,N.,
            Mohanakrishnan,M., Snowball,J., Ediga,H.H., Madala,S.K. and
            Sinner,D.
  TITLE     Wnt signaling regulates ion channel expression to promote smooth
            muscle and cartilage formation in developing mouse trachea
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 325 (6), L788-L802 (2023)
   PUBMED   37873566
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Tu,L., Bean,J.C., He,Y., Liu,H., Yu,M., Liu,H., Zhang,N., Yin,N.,
            Han,J., Scarcelli,N.A., Conde,K.M., Wang,M., Li,Y., Feng,B.,
            Gao,P., Cai,Z.L., Fukuda,M., Xue,M., Tong,Q., Yang,Y., Liao,L.,
            Xu,J., Wang,C., He,Y. and Xu,Y.
  TITLE     Anoctamin 4 channel currents activate glucose-inhibited neurons in
            the mouse ventromedial hypothalamus during hypoglycemia
  JOURNAL   J Clin Invest 133 (14), e163391 (2023)
   PUBMED   37261917
  REMARK    GeneRIF: Anoctamin 4 channel currents activate glucose-inhibited
            neurons in the mouse ventromedial hypothalamus during hypoglycemia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4177)
  AUTHORS   Reichhart,N., Schoberl,S., Keckeis,S., Alfaar,A.S., Roubeix,C.,
            Cordes,M., Crespo-Garcia,S., Haeckel,A., Kociok,N., Fockler,R.,
            Fels,G., Mataruga,A., Rauh,R., Milenkovic,V.M., Zuhlke,K.,
            Klussmann,E., Schellenberger,E. and Strauss,O.
  TITLE     Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation
            channel
  JOURNAL   Sci Rep 9 (1), 2257 (2019)
   PUBMED   30783137
  REMARK    GeneRIF: Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective
            cation channel.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4177)
  AUTHORS   Suzuki,J., Fujii,T., Imao,T., Ishihara,K., Kuba,H. and Nagata,S.
  TITLE     Calcium-dependent phospholipid scramblase activity of TMEM16
            protein family members
  JOURNAL   J Biol Chem 288 (19), 13305-13316 (2013)
   PUBMED   23532839
REFERENCE   5  (bases 1 to 4177)
  AUTHORS   Duran,C., Qu,Z., Osunkoya,A.O., Cui,Y. and Hartzell,H.C.
  TITLE     ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are
            intracellular proteins
  JOURNAL   Am J Physiol Cell Physiol 302 (3), C482-C493 (2012)
   PUBMED   22075693
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF535597.1, AK078364.1, BC065114.1 and AI463477.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the supported protein.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-236               CF535597.1         1-236
            237-1811            AK078364.1         1-1575
            1812-3657           BC065114.1         1769-3614
            3658-4152           AI463477.1         1-495               c
            4153-4177           BC065114.1         4110-4134
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 44.42 cM"
     gene            1..4177
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /note="anoctamin 4"
                     /db_xref="GeneID:320091"
                     /db_xref="MGI:MGI:2443344"
     exon            1..359
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            360..554
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            555..659
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            660..796
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            797..955
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1056
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1101
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1233
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1340
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1396
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1518
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1653
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1750
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1811
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1894
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2035
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2200
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2237
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2349
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2407
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2505
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2648
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2649..2801
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2949
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3069
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3070..3175
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3176..3371
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3372..4160
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4132..4137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /note="hexamer: AATAAA"
     polyA_site      4160
                     /gene="Ano4"
                     /gene_synonym="A330096O15Rik; Gm65; Tmem16d"
                     /note="major polyA site"
ORIGIN      
        1 cccaaacctt gctttccaaa gcctagcctg ctgcccccgc ccccctaccc catgccgccc
       61 ctgcacccgg tggggaggac ctgtcctggg agtgatgctc tgagactgtg gtgaaattgg
      121 tccccggctg gctgctttca ctccggcttg cttgctctcc ggagagtcct gttccagact
      181 tctcgggggt tttacagcac aaacaactgc atgcgggctg gaggggaggc tgaaaggact
      241 tgtgtgtgga gagctttgct gcctcccatc actttgccga ggttcgtggt gaatttctgt
      301 ctaccacaga caccgaggaa ggatcctttc tccttctgtt gactctttct atttttatgg
      361 tttaagttta tctattcatg gggctgaaaa gcgcttgcaa atccatcaac ggcgaagtgt
      421 ggcaagccgc ccagggtcac gtcgtcgcct gcctgtggtc aggcattcct cactcccacc
      481 aggcagacgg tcaataaaaa tggaggccag ctcttctgga atcactaatg gaaaaaataa
      541 agttttccac gcagaaggtg gactggattt gcaaagccat cagctggata tgcaaatact
      601 gcccgacggc ccaaagagcg atgtggactt ttcagagatt cttaatgcaa tacaagaaat
      661 ggccaaggat gtcaatattc tttttgacga acttgaggct gtcaacagcc cttgcaaaga
      721 tgatgattct ctgcttcacc ctggaaatct gacgagcact tcggaggaca ccagcaggct
      781 agaagcaggg ggagagacag tgcgagaacg aaacaaatcc aatggacttt actttagaga
      841 cggaaaatgt cgaattgact acattcttgt gtacagaaaa tccaaccctc agactgaaaa
      901 gagagaagtg tttgaaagaa atattagagc agaaggatta caaatggaga aagagtcctc
      961 tctaataaat agtgacatta tctttgtgaa gttgcatgcc ccatgggaag tccttggaag
     1021 atatgcagag caaatgaatg tacggatgcc tttcaggaga aaaatctatt acctgccccg
     1081 ccggtacaag ttcatgagca ggatcgataa acaaataagc aggttccgga gatggttacc
     1141 taagaagcca atgaggctgg acaaggagac actgccagac ctggaagaga atgactgcta
     1201 cactgcccct ttcagccaac aaaggatcca tcacttcatc atacacaaca aagacacttt
     1261 cttcaacaat gccacaagaa gcagaatcgt gcatcatatt ttacaaagaa taaagtacga
     1321 ggaaggaaag aataaaatcg gtctgaaccg cttgcttacc aatggctcct atgaagctgc
     1381 ctttcccctg catgagggaa gttacaggag taaaaactcc attaaaaccc atggagctgt
     1441 aaaccaccga catctactgt atgagtgctg ggcctcctgg ggcgtgtggt acaagtacca
     1501 gcctttggat cttgtcaggc gatattttgg agagaagatt ggactgtatt tcgcctggct
     1561 gggctggtac acaggcatgc tcttccccgc agccttcatt gggttgtttg tcttcttgta
     1621 tggagtcacc actctggatc actgccaagt cagtaaagaa gtctgccaag caacagatat
     1681 catcatgtgt cctgtatgtg acaaatactg tccattcatg aggctttcag acagctgtgt
     1741 ctatgccaag gtgacccacc tttttgacaa tggggccact gtgttctttg ctgtgttcat
     1801 ggcagtctgg gctactgttt tcctggaatt ctggaagaga cgccgagcag tgatcgctta
     1861 tgactgggat ctgattgact gggaagaaga ggaggaagaa atacgacccc agtttgaagc
     1921 caagtattcc aagaaagagc ggatgaatcc catttcagga aagccagaac cttatcaagc
     1981 atttactgat aaatgcagca gacttatcgt ttctgcatca ggaatattct tcatgatctg
     2041 cgtggtgatc gctgctgtgt tcgggatcgt catttacagg gtggtgactg tgagcacttt
     2101 cgctgccttt aagtgggcat taatcaggaa taactctcag gttgcaacca cagggactgc
     2161 agtatgcatc aacttttgta tcattatgct gctgaatgtg ctctatgaaa aagtcgcact
     2221 ccttctaaca aatctggaac agcctcgcac agaatccgag tgggagaaca gctttacctt
     2281 gaaaatgttt ctttttcagt ttgtcaatct gaacagctcc actttttaca tcgcattctt
     2341 cctcggaaga tttacaggac acccaggtgc ctacttgaga ttgataaaca ggtggaggct
     2401 agaagagtgc caccctagcg gatgccttat tgatctctgc atgcaaatgg gtattataat
     2461 ggtgctaaag cagacctgga ataatttcat ggaacttggc tacccgttaa ttcaaaattg
     2521 gtggactaga agaaaagtcc gacaagaaca tgggactgaa aggaaaataa atttcccaca
     2581 gtgggaaaag gactacaatc ttcagccgat gaatgcctat ggactcttcg atgaatacct
     2641 ggaaatgatc cttcagttcg gattcacaac gatctttgtg gcagcttttc ctctagcacc
     2701 gcttctggcc ttactgaata acatcattga aatccgactt gatgcttata agtttgttac
     2761 tcagtggaga aggcctttgg cttcgagagc caaagacata ggaatttggt atggaattct
     2821 cgaaggcatt gggattctgt ctgttattac aaatgcattt gtcatagcaa taacatccga
     2881 cttcatccct cgcttagtat atgcttataa atatggacct tgtgcaggcc aaggagaagc
     2941 tgggcaaaag tgcatggttg gctatgtgaa tgccagcttg tctgtgttcc gaatttctga
     3001 ctttgaaaat cggtcggagc cagagtctga tggcagtgag ttctcaggaa cccctctgaa
     3061 gtactgcaga tatcgagact accgggaccc tcctcactct ttggcaccct atggctacac
     3121 actgcagttc tggcatgttc tcgcagctcg gctggctttt atcattgtgt ttgagcacct
     3181 cgtgttttgt ataaagcacc tcatttccta tctgatacca gacctcccga aagatctaag
     3241 ggaccggatg aggagagaga agtacttgat ccaggagatg atgtatgagg ctgagctgga
     3301 gcgcctccag aaagaacgga aggagaggaa gaagaacgga aaagcacatc acaatgagtg
     3361 gccatgacaa ggtgacagtt gattttcggg ctaaagacct gaattttgtt tacttctggc
     3421 agtgcaaaaa agcacattca agtgaatgac cagcagcgca cgcgcagtgc atgctgccga
     3481 caccaccgcg cagccgcaga cagggcggca gcgaggagat ggccgactga ggtggagtcc
     3541 cgctgttgtg aaatcacgct gcagtccagc acacaattgc tatctatcca tagaccattc
     3601 ttgaccgagc aagcatgcac gttatgggca gttacattct caagttttta aaaccaaggg
     3661 gagacttgta tactgggcct gtttttttag cctgtttgct accttttttg cattctatcc
     3721 catgtgaatt ttacagacac tgggcttaaa aagggtattt ggacacttgg acacgttcct
     3781 agaatgtcct gttctacatc accaaatgac acagagaaga ccctgtttac aacttcttct
     3841 ttctttttaa gttcagatct ttctgaagag attattctat atgacatatc tatagctatg
     3901 tggatggcca tagatgtatt tctgtgtgta catatgtata gacatgtatt cctacatatg
     3961 tacatacaaa tgcagaggta tataaagtac ataagaattc cttacttgta aatagccaaa
     4021 attaaaaaat aaaaaacagt actgacatga atgatgaatt ttcacattta aagagtcacc
     4081 aacatgaagc catgtttaat tgcttgtata atgtgacaca atagagttta aaataaattt
     4141 atgcacatgg aatattttca aaaaaaaaaa aaaaaaa
//
