LOCUS       NM_001364562            3720 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus anoctamin 2 (Ano2), transcript variant 2, mRNA.
ACCESSION   NM_001364562 XM_011241354
VERSION     NM_001364562.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Guarneri,G., Pifferi,S., Dibattista,M., Reisert,J. and Menini,A.
  TITLE     Paradoxical electro-olfactogram responses in TMEM16B knock-out mice
  JOURNAL   Chem Senses 48 (2023)
   PUBMED   36744918
  REMARK    GeneRIF: Paradoxical electro-olfactogram responses in TMEM16B
            knock-out mice.
REFERENCE   3  (bases 1 to 3720)
  AUTHORS   Maul,A., Huebner,A.K., Strenzke,N., Moser,T., Rubsamen,R.,
            Jovanovic,S. and Hubner,C.A.
  TITLE     The Cl--channel TMEM16A is involved in the generation of cochlear
            Ca2+ waves and promotes the refinement of auditory brainstem
            networks in mice
  JOURNAL   Elife 11, e72251 (2022)
   PUBMED   35129434
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3720)
  AUTHORS   Hernandez-Clavijo,A., Sarno,N., Gonzalez-Velandia,K.Y., Degen,R.,
            Fleck,D., Rock,J.R., Spehr,M., Menini,A. and Pifferi,S.
  TITLE     TMEM16A and TMEM16B Modulate Pheromone-Evoked Action Potential
            Firing in Mouse Vomeronasal Sensory Neurons
  JOURNAL   eNeuro 8 (5) (2021)
   PUBMED   34433575
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3720)
  AUTHORS   Auer,F., Franco Taveras,E., Klein,U., Kesenheimer,C.,
            Fleischhauer,D., Mohrlen,F. and Frings,S.
  TITLE     Anoctamin 2-chloride channels reduce simple spike activity and
            mediate inhibition at elevated calcium concentration in cerebellar
            Purkinje cells
  JOURNAL   PLoS One 16 (3), e0247801 (2021)
   PUBMED   33651839
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3720)
  AUTHORS   Pifferi,S., Dibattista,M. and Menini,A.
  TITLE     TMEM16B induces chloride currents activated by calcium in mammalian
            cells
  JOURNAL   Pflugers Arch 458 (6), 1023-1038 (2009)
   PUBMED   19475416
  REMARK    GeneRIF: Our results demonstrate that mTMEM16B functions as a
            Ca(2+)-activated Cl(-) channel when expressed in HEK 293T cells.
REFERENCE   7  (bases 1 to 3720)
  AUTHORS   Stephan,A.B., Shum,E.Y., Hirsh,S., Cygnar,K.D., Reisert,J. and
            Zhao,H.
  TITLE     ANO2 is the cilial calcium-activated chloride channel that may
            mediate olfactory amplification
  JOURNAL   Proc Natl Acad Sci U S A 106 (28), 11776-11781 (2009)
   PUBMED   19561302
  REMARK    GeneRIF: ANO2 is the cilial calcium-activated chloride channel that
            may mediate olfactory amplification.
            GeneRIF: Ano2 likely amplifies the odor-induced generator potential
            in olfactory sensory neuron cilia by sensing elevated calcium
            levels, permitting the outward flow of chloride ions from the cell.
REFERENCE   8  (bases 1 to 3720)
  AUTHORS   Stohr,H., Heisig,J.B., Benz,P.M., Schoberl,S., Milenkovic,V.M.,
            Strauss,O., Aartsen,W.M., Wijnholds,J., Weber,B.H. and Schulz,H.L.
  TITLE     TMEM16B, a novel protein with calcium-dependent chloride channel
            activity, associates with a presynaptic protein complex in
            photoreceptor terminals
  JOURNAL   J Neurosci 29 (21), 6809-6818 (2009)
   PUBMED   19474308
  REMARK    GeneRIF: TMEM16B is lost from photoreceptor membranes of
            MPP4-deficient mice
REFERENCE   9  (bases 1 to 3720)
  AUTHORS   Rock,J.R. and Harfe,B.D.
  TITLE     Expression of TMEM16 paralogs during murine embryogenesis
  JOURNAL   Dev Dyn 237 (9), 2566-2574 (2008)
   PUBMED   18729231
  REMARK    GeneRIF: Data show the expression of Tmem16b during murine
            embryogenesis with an emphasis on the respiratory, digestive,
            skeletal, and integumentary systems.
REFERENCE   10 (bases 1 to 3720)
  AUTHORS   Ding,S., Wu,X., Li,G., Han,M., Zhuang,Y. and Xu,T.
  TITLE     Efficient transposition of the piggyBac (PB) transposon in
            mammalian cells and mice
  JOURNAL   Cell 122 (3), 473-483 (2005)
   PUBMED   16096065
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            KC164759.2.
            
            On Jun 22, 2018 this sequence version replaced XM_011241354.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: KC164759.2, SRR17784643.354786.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3720              KC164759.2         201-3920
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.58 cM"
     gene            1..3720
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /note="anoctamin 2"
                     /db_xref="GeneID:243634"
                     /db_xref="MGI:MGI:2387214"
     exon            1..126
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..3101
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="anoctamin-2 isoform 2"
                     /protein_id="NP_001351491.1"
                     /db_xref="GeneID:243634"
                     /db_xref="MGI:MGI:2387214"
                     /translation="MAAPGLRDIPLLPGSPRRLSSRTVARGSQGPKHGQQYLKVPGHR
                     APGQRDNSSLHPSQVSRRESSRDRSVINNYLDANEPPSSEARLSRMHFHDNQRKVDYV
                     LAYHYRKRGAHLGHGSPGHSLAVISNGETGKERHGGGPGDVELGPLDALEEERREQRD
                     EFEHNLMAAGLELEKDLESKSQGSVFVRIHAPWQVLAREAEFLKIKVPTKKMYEIKAG
                     GSIAKKFSAILQTLSSPLQPRVPEHSNNRMKNLSYPFSREKMYLYNIQEKDTFFDNAT
                     RSRIVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPGDDMNDRKLLYQ
                     EWARYGVFYKFQPIDLIRKYFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIE
                     EDIPSKEMCDHQNAFTMCPLCDKSCDYWNLSSACGTARASHLFDNPATVFFSIFMALW
                     ATMFLENWKRLQMRLGYFWDLTGIEEEEEHSRPEYETKVREKLLKESGKSAVQKLEAN
                     SPEDDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRITTAAALSLNKA
                     TRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLL
                     KFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGK
                     QLIQNNIFEIGVPKLKKLFRKLKDETEPGESDPDHSKRPEQWDLDHSLEPYTGLTPEY
                     MEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWF
                     DILSGIGKFSVIINAFVIAVTSDFIPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLKE
                     GTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWSVLSARLAFVIIFQNLVMFL
                     SVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKVKLADEPTQRSQGGGDRSRRS
                     RAASSAPSGRSQPGSIASSGSQHTNV"
     exon            127..311
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            312..638
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            639..737
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            738..889
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            890..944
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            945..996
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1052
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1094
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1159
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1294
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1455
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1538
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1646
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1721
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1917
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2029
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2087
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2188
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2334
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2487
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2540
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2722
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2828
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..3720
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3686..3691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /note="hexamer: AATAAA"
     polyA_site      3702
                     /gene="Ano2"
                     /gene_synonym="Tmem16b"
                     /note="major polyA site"
ORIGIN      
        1 agacagagct gagccgcgat ggaattaact ccctggcagc cacataagcc tatccagagt
       61 ggagcgaggg cagccaaggg gcttggcatc tggggaccag caccatggcg gcccctgggc
      121 tgcgagacat ccctctgctc cctggatctc cacgtcggct gagttctcgg accgtggcca
      181 gaggaagcca gggccccaaa catgggcagc agtacctcaa agtgccaggt caccgggccc
      241 caggtcagag ggacaattcc agcctacacc ctagccaggt ctccaggaga gagagttccc
      301 gagaccgttc tgtcatcaat aactacctgg atgccaatga gcccccgtcc tcggaagccc
      361 gtctgagccg catgcacttt cacgacaacc agaggaaagt cgactatgta cttgcatacc
      421 actaccggaa acgaggcgca cacctgggtc acggctcccc tggacactct ctggctgtta
      481 tctccaatgg ggagacaggc aaggagcgcc atggcggggg ccctggtgat gttgaacttg
      541 ggccactgga tgctctggaa gaggaaagga gggagcagcg agatgaattt gagcacaacc
      601 tgatggctgc tgggctggag ctggagaagg acttggagag caaaagccag ggatctgtct
      661 ttgtccggat ccacgcccca tggcaggtgc tggccaggga agcagagttc ttgaagatca
      721 aagtccccac caagaaaatg tacgagatca aagcaggagg cagcatcgca aagaagttca
      781 gcgccattct gcagacgctc agttcacccc tgcagcccag agttcctgaa cacagcaaca
      841 acaggatgaa aaacctctcc tacccattct ccagggaaaa aatgtacctg tacaacatcc
      901 aggaaaagga caccttcttt gacaatgcta cccggagccg cattgtacac gagattctga
      961 agcggacggc ctgttctcga gccaacaaca cgatgggtat taactctcta atagcaaaca
     1021 atatctacga ggctgcctac ccgcttcatg atggtgaata tgacagtcca ggggatgaca
     1081 tgaatgacag aaagctgctg tatcaggaat gggcacgcta tggagtgttt tacaaatttc
     1141 aacccattga cctcatcagg aagtattttg gagagaaaat tgggctatat tttgcttggc
     1201 tggggttgta tacatcattc ctgatcccat cttctgtcat aggcgtgatt gtgtttcttt
     1261 atggatgtgc aacaatcgag gaagatattc ccagcaaaga gatgtgtgac caccagaatg
     1321 ccttcaccat gtgtcccctg tgtgacaagt cctgtgacta ttggaacctc agctcagcct
     1381 gtgggactgc acgggccagt catctgtttg acaatccagc caccgtcttc ttttccatct
     1441 tcatggctct gtgggctacc atgtttctgg aaaactggaa gagactccag atgcggttgg
     1501 gctacttctg ggacctgact gggatcgaag aggaagaaga acactctcgg cctgaatatg
     1561 aaaccaaagt acgagagaaa ctgttaaagg agagtggcaa gtcagcggtt cagaagctgg
     1621 aagcaaattc acctgaggat gatgaggatg atgaagataa actgacttgg aaagatcgtt
     1681 tcccaggata cttgatgaac tttgcctcca tcttgtttat gattgccctg acgttctcca
     1741 ttgtctttgg ggtcattgtg tatcggatca caactgcagc tgccctgtct ctcaacaagg
     1801 ccacacgctc caatgtccgg gtgacagtga ctgcaacagc agtcatcatc aacctcgtgg
     1861 tcatcctcat cttggatgag atctatggtg ctgtggccaa gtggcttacc aaaatcgagg
     1921 ttccgaaaac agaacagact tttgaagaac ggttgatcct caaagctttc ttgctcaagt
     1981 ttgtcaatgc ctactccccc atcttctatg tggccttttt caaggggagg tttgtgggca
     2041 gacctggaag ctatgtctac gtgttcgacg gttaccgcat ggaagagtgt gctccgggag
     2101 gctgtctcat ggagctgtgc atccaactca gcatcatcat gttgggcaag cagctgatcc
     2161 agaacaatat ctttgagatt ggagtcccga agctaaagaa actctttcgg aagctgaaag
     2221 atgagacaga gcctggagaa agtgaccctg accactcaaa acgtccagag cagtgggacc
     2281 tggaccacag cctagagcct tacactgggc tgactcctga gtacatggaa atgatcatcc
     2341 agtttggttt tgtcaccctt tttgtggcct cctttcctct ggcccctgtg tttgctctcc
     2401 tcaacaatgt cattgaagtg cggctggatg caaaaaagtt tgttacagaa ctgagacgcc
     2461 cagatgctgt gaggaccaaa gacattggaa tttggtttga cattctctct ggaattggca
     2521 agttctccgt catcattaat gcttttgtca ttgctgtcac ctccgacttt atcccccgcc
     2581 ttgtgtacca gtactcctat agccacaacg ggacactaca tggctttgtg aaccatacac
     2641 tctccttttt caacgtcagt cagctgaagg agggaacaca gcctgaaaac tcacagttcg
     2701 accaggaggt tcagttttgc aggttcaaag attatcgaga accaccatgg gcaccaaacc
     2761 cttatgagtt ttccaagcag tactggtctg ttttgtctgc ccgtctggcc tttgttatca
     2821 tcttccagaa cctagtgatg ttcctgagtg tcctggtgga ctggatgatt ccggatatcc
     2881 ccactgacat cagcgaccag atcaagaaag agaagagctt gctggtagac ttctttctga
     2941 aggaggagca tgagaaggtc aagctggctg atgagcccac ccagaggagc cagggaggtg
     3001 gggatcggag caggaggagc cgtgcagcca gctctgcacc atcaggccgg agccagccag
     3061 gcagcatagc atcctcaggg tcccagcaca ccaatgtatg agtcattggc agcagaggag
     3121 ggcatgttat ggcgaaacgc atgagtacac catatgttcc tataacacag tggtacctct
     3181 tctgctggga actggcttaa gggtgtctcc aaaaggaagt tacagaaagg gaaatagagt
     3241 gagagagtgc aggagggaag gaagccgtat agcttctcaa aaaagctaga agcttgtgtt
     3301 tcttttaggt tgacttttga gctgtgtcct ttcagggagt tggtacccca tgggggtcac
     3361 ttcaccatag aattttctcg ggtcctttct tattcttcac catcatctta ggtgaaagaa
     3421 gttcagtgag ggtcccatcc atcctagcac ccatctaact gatcacaccc ccttcctact
     3481 gtcaccctgg aataacctct cagagacact tgtgaatggg gccacaacaa ggctgggcag
     3541 atgcaaatga agttacagga ttgatttctg tggccactgg acctggtggt aaggaagaaa
     3601 ggtcctgtcc aggtttgtct gcagctccta tgacaaggat gcatataatg caactcacca
     3661 ccgttttatg caagttctgg tttaaaataa aataaatgga aagtcttttt tttaaaaaaa
//
