LOCUS       NM_178642               4582 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus anoctamin 1, calcium activated chloride channel
            (Ano1), transcript variant 1, mRNA.
ACCESSION   NM_178642
VERSION     NM_178642.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Sun,Z., Shan,X., Fan,C., Liu,L., Li,S., Wang,J., Zhou,N., Zhu,M.
            and Chen,H.
  TITLE     TMEM16A regulates satellite cell-mediated skeletal muscle
            regeneration by ensuring a moderate level of caspase 3 activity
  JOURNAL   Stem Cells 42 (10), 902-913 (2024)
   PUBMED   39097775
  REMARK    GeneRIF: TMEM16A regulates satellite cell-mediated skeletal muscle
            regeneration by ensuring a moderate level of caspase 3 activity.
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Garrud,T.A.C., Bell,B., Mata-Daboin,A., Peixoto-Neves,D.,
            Collier,D.M., Cordero-Morales,J.F. and Jaggar,J.H.
  TITLE     WNK kinase is a vasoactive chloride sensor in endothelial cells
  JOURNAL   Proc Natl Acad Sci U S A 121 (15), e2322135121 (2024)
   PUBMED   38568964
REFERENCE   3  (bases 1 to 4582)
  AUTHORS   Raghavan,S., Brishti,M.A., Bernardelli,A., Mata-Daboin,A.,
            Jaggar,J.H. and Leo,M.D.
  TITLE     Extracellular glucose and dysfunctional insulin receptor signaling
            independently upregulate arterial smooth muscle TMEM16A expression
  JOURNAL   Am J Physiol Cell Physiol 326 (4), C1237-C1247 (2024)
   PUBMED   38581667
  REMARK    GeneRIF: Extracellular glucose and dysfunctional insulin receptor
            signaling independently upregulate arterial smooth muscle TMEM16A
            expression.
REFERENCE   4  (bases 1 to 4582)
  AUTHORS   Liu,X.M., Li,J., Chen,D., Li,H., Qin,X.Y., Wang,Y.X., Gu,Y.Z.,
            Li,N., Zhou,L.G. and Feng,M.
  TITLE     Ano1 regulates embryo transport by modulating intracellular calcium
            levels in oviduct smooth muscle
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (4), 167059 (2024)
   PUBMED   38336104
  REMARK    GeneRIF: Ano1 regulates embryo transport by modulating
            intracellular calcium levels in oviduct smooth muscle.
REFERENCE   5  (bases 1 to 4582)
  AUTHORS   Sciancalepore,M., Ragnini,A., Zacchi,P., Borelli,V., D'Andrea,P.,
            Lorenzon,P. and Bernareggi,A.
  TITLE     A Pharmacological Investigation of the TMEM16A Currents in Murine
            Skeletal Myogenic Precursor Cells
  JOURNAL   Int J Mol Sci 25 (4), 2225 (2024)
   PUBMED   38396901
  REMARK    GeneRIF: A Pharmacological Investigation of the TMEM16A Currents in
            Murine Skeletal Myogenic Precursor Cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4582)
  AUTHORS   Yang,Y.D., Cho,H., Koo,J.Y., Tak,M.H., Cho,Y., Shim,W.S.,
            Park,S.P., Lee,J., Lee,B., Kim,B.M., Raouf,R., Shin,Y.K. and Oh,U.
  TITLE     TMEM16A confers receptor-activated calcium-dependent chloride
            conductance
  JOURNAL   Nature 455 (7217), 1210-1215 (2008)
   PUBMED   18724360
  REMARK    GeneRIF: transmembrane protein 16A (TMEM16A, which we also call
            anoctamin 1 (ANO1)) is a bona fide Ca(2+)-activated chloride
            channel that is activated by intracellular Ca(2+) and
            Ca(2+)-mobilizing stimuli
REFERENCE   7  (bases 1 to 4582)
  AUTHORS   Rock,J.R., Futtner,C.R. and Harfe,B.D.
  TITLE     The transmembrane protein TMEM16A is required for normal
            development of the murine trachea
  JOURNAL   Dev Biol 321 (1), 141-149 (2008)
   PUBMED   18585372
  REMARK    GeneRIF: Study reports that all mice homozygous for a null allele
            of Tmem16a died within one month of birth and exhibited severe
            tracheomalacia with gaps in the tracheal cartilage rings along the
            entire length of the trachea.
REFERENCE   8  (bases 1 to 4582)
  AUTHORS   Rock,J.R., Lopez,M.C., Baker,H.V. and Harfe,B.D.
  TITLE     Identification of genes expressed in the mouse limb using a novel
            ZPA microarray approach
  JOURNAL   Gene Expr Patterns 8 (1), 19-26 (2007)
   PUBMED   17911046
REFERENCE   9  (bases 1 to 4582)
  AUTHORS   Visel,A., Carson,J., Oldekamp,J., Warnecke,M., Jakubcakova,V.,
            Zhou,X., Shaw,C.A., Alvarez-Bolado,G. and Eichele,G.
  TITLE     Regulatory pathway analysis by high-throughput in situ
            hybridization
  JOURNAL   PLoS Genet 3 (10), 1867-1883 (2007)
   PUBMED   17953485
REFERENCE   10 (bases 1 to 4582)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122336.4 and AC153792.4.
            
            On Dec 11, 2019 this sequence version replaced NM_178642.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052589.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC122336.4         177198-177408       c
            212-357             AC153792.4         192016-192161       c
            358-690             AC153792.4         182725-183057       c
            691-789             AC153792.4         170397-170495       c
            790-941             AC153792.4         168981-169132       c
            942-996             AC153792.4         167586-167640       c
            997-1048            AC153792.4         163885-163936       c
            1049-1104           AC153792.4         161406-161461       c
            1105-1146           AC153792.4         158090-158131       c
            1147-1211           AC153792.4         151882-151946       c
            1212-1346           AC153792.4         150457-150591       c
            1347-1507           AC153792.4         146978-147138       c
            1508-1590           AC153792.4         135050-135132       c
            1591-1602           AC153792.4         132857-132868       c
            1603-1674           AC153792.4         132231-132302       c
            1675-1749           AC153792.4         128232-128306       c
            1750-1951           AC153792.4         124996-125197       c
            1952-2063           AC153792.4         124717-124828       c
            2064-2121           AC153792.4         124225-124282       c
            2122-2222           AC153792.4         122232-122332       c
            2223-2368           AC153792.4         121300-121445       c
            2369-2521           AC153792.4         116823-116975       c
            2522-2574           AC153792.4         110563-110615       c
            2575-2759           AC153792.4         108824-109008       c
            2760-2865           AC153792.4         106083-106188       c
            2866-4582           AC153792.4         101931-103647       c
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.85 cM"
     gene            1..4582
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /note="anoctamin 1, calcium activated chloride channel"
                     /db_xref="GeneID:101772"
                     /db_xref="MGI:MGI:2142149"
     exon            1..211
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            212..357
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     CDS             250..3132
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane protein 16A; anoctamin-1"
                     /codon_start=1
                     /product="anoctamin-1 isoform 1"
                     /protein_id="NP_848757.5"
                     /db_xref="CCDS:CCDS40202.2"
                     /db_xref="GeneID:101772"
                     /db_xref="MGI:MGI:2142149"
                     /translation="MRVPEKYSTLPAEDRSVHIVNICAIEDLGYLPSEGTLLNSLSVD
                     PDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRGLQNDMVLGTRSVRQDQPLP
                     GKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHA
                     PWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTK
                     RLSYPFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGV
                     YSAAYPLHDGDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAW
                     LGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPLCDKTCSYWKMS
                     SACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAV
                     KDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIV
                     LGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTK
                     IEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEE
                     CAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVK
                     RKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAK
                     KFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQN
                     GTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKD
                     FWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQG
                     KQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL"
     exon            358..690
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            691..789
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            790..941
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            942..996
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1048
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1104
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1146
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1211
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1346
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1507
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1590
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1602
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1674
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1749
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1951
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2063
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2121
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2222
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2368
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2521
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2574
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2759
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2865
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..4582
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4564..4569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /note="hexamer: AATAAA"
     polyA_site      4582
                     /gene="Ano1"
                     /gene_synonym="Tmem16a"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcagct gggctcaacc ttcctcccgc ctctgccggt ccctctgctg gacccagggc
       61 gtccggagcg aacacatcat gcaggacgcg caggacagcg acatcggcct ggagggcttg
      121 accccagagg gaacctctgc ggaccgagag tgccagcggg gaccggaaac aatcgcgcac
      181 gaggcacagg acgcaggaac ccccaactca gacgccaccg gggttgtgga tggggagcgc
      241 gaggccacca tgagggtccc cgagaagtac tcgacgctcc cggcggagga ccgcagcgtc
      301 cacatcgtga acatctgcgc catcgaggac ctgggctacc tgccgtccga gggcacgttg
      361 ctgaactctc tgtccgtgga ccccgacgcc gaatgcaagt atggactata cttcagggat
      421 ggcaaacgga aggtggacta catcttggta taccatcaca agagagcctc agggagcagg
      481 actctggcca ggaggggact acaaaatgac atggtcctgg ggacccgcag cgtcaggcag
      541 gaccagcccc ttcccgggaa ggggagccct gtggatgcag gctcaccgga agtccccatg
      601 gattaccatg aagatgacaa acgcttcaga cgggaggaat atgagggcaa cctgttggag
      661 gcaggcctgg agttggagaa tgacgaggat accaaaatcc atggtgtcgg gtttgtgaag
      721 atccatgcgc cctggcatgt gctctgtagg gaagctgagt ttttgaaact aaagatgccc
      781 acaaagaagg tgtaccacat cagtgagacg cgaggcctcc tgaaaaccat caactcggtt
      841 ctgcagaaga tcacagaccc catccagccc aaggtggctg agcacaggcc acagaccaca
      901 aagaggctct cctatccctt ctcccgggag aagcaacacc tattcgacct gactgacagg
      961 gactcttttt tcgacagcaa aacccggagc acaatagtct atgaaatcct gaagagaaca
     1021 acgtgcacca aggccaagta cagcatgggt atcaccagcc tcctggccaa tggcgtatac
     1081 tcagctgcat accctctgca cgatggggac tatgagggtg acaacgttga gttcaacgac
     1141 aggaaactcc tgtatgagga atgggcaagt tacggagtct tctacaaata ccagcccatt
     1201 gacctggtca ggaaatactt tggtgagaag gttggcctgt actttgcctg gcttggagcc
     1261 tacacccaga tgctcatccc tgcctcgatc gtgggtgtca ttgtctttct ctatggatgt
     1321 gccactgtgg acgaaaacat ccccagtatg gagatgtgtg accagagata caacatcacc
     1381 atgtgtcctc tgtgtgacaa gacctgcagc tactggaaga tgagctcagc ctgtgccaca
     1441 gcccgtgcca gtcacctttt tgataaccct gccaccgtct tcttctctgt gtttatggcc
     1501 ctctgggctg ccactttcat ggagcactgg aaacggaagc agatgaggct caactaccga
     1561 tgggacctca caggcttcga ggaggaggag gaagctgtca aggatcatcc cagagcagag
     1621 tatgaagcca gagtcttaga gaagtcactg agaaaagaat ccagaaacaa agagaccgac
     1681 aaggtgaagc tgacctggag ggaccgattc ccagcctatt tcaccaatct tgtctccatc
     1741 atcttcatga tcgcagtgac atttgcaatc gtcctcggag ttatcatcta tagaatctcc
     1801 acagctgcag ccttggccat gaactcctcc ccgtctgtgc ggtccaacat ccgggttaca
     1861 gtcacggcca ccgctgttat catcaacctc gtggtcatca ttctgctgga tgaagtttac
     1921 ggctgcattg ccaggtggct caccaagatt gaggtcccaa agacagagaa gagctttgag
     1981 gagaggctaa ccttcaaggc cttcctgctc aagtttgtga actcttacac tcccatcttc
     2041 tatgtcgcct tcttcaaagg ccggtttgtt ggtcggcccg gtgactacgt gtacatcttc
     2101 cgctctttcc ggatggagga gtgtgccccg ggcggctgcc tcatggagct ctgtatccag
     2161 ctgagcatca ttatgctggg caagcagcta atccagaaca atctcttcga gattggcatc
     2221 ccgaagatga aaaagttcat ccgctacctg aagctgcgca gacagagccc ctcagaccgt
     2281 gaagagtacg tgaagcggaa gcagcgctat gaggtggact tcaacctcga acctttcgcc
     2341 ggcctcacgc ccgagtacat ggaaatgatc attcagttcg gctttgtcac cctgtttgtt
     2401 gcgtccttcc ctctggctcc actcttcgcc ctgctaaaca acatcattga gatccgcctg
     2461 gatgccaaaa agtttgtcac cgagctacgg aggccagtag ccatcagagc caaagacatc
     2521 ggcatctggt ataacatcct cagaggtgtt gggaagctgg ctgtcatcat taatgccttt
     2581 gtgatctcct tcacgtctga cttcatccct cgcctggtgt acctctacat gtacagtcag
     2641 aatgggacca tgcacggctt cgtcaatcac acgctctctt ccttcaatgt cagcgacttc
     2701 cagaatggca cagcacccaa tgacccactg gacctgggct atgaggttca gatctgcagg
     2761 tataaagatt accgggaacc cccatggtca gaacacaagt atgacatctc caaagacttc
     2821 tgggctgtcc tggccgcccg actggcattt gtcattgtct tccagaacct ggtgatgttc
     2881 atgagtgact ttgtggactg ggtgatccct gatatcccca aagacatcag ccagcagatc
     2941 cacaaagaga aggttctcat ggtggagctg tttatgcgtg aggagcaggg caagcagcag
     3001 ctactggaca catggatgga gaaggagaag ccaagggatg tgccttgtaa caaccacagc
     3061 cccacaaccc acccagaggc aggcgacggc agcccagtcc ccagctacga gtaccatggg
     3121 gacgcgctgt agccatgatg acaggccagc aggccagcag gcttctgacc atcaccaagt
     3181 gactactcgg cttcctccaa agccagcact tctagcccca ccagggccta tggctcttgt
     3241 gtcgtctgga cgtgtggagg agcactctga tgagacaacg gtcttctttc ttctttctgt
     3301 ttcttctcat ttttgcacaa ggccattatg cagggaagtt tttatcttta gtattcaatg
     3361 ccaaggatgg ctggtgatgg ggatgaacag ggtgaggcat gttccacaga catatggagg
     3421 aatatagagc ccccctgtgg tggtagggag ccaagtcagc atccttagtc tgaaggatgc
     3481 cctgggatag atgcagaatg gtacctagga acctgctgac attttgcaga agggtgaaca
     3541 actcagactc agattcccaa agatgtcacc tgctctagct ggacaagctg gaagactcca
     3601 ctgtctttcc ttctgggagc tcagaagttc agacctgggg tcagaaacag aggtgcctga
     3661 ccacccacac agcacacaca tatggtggct tcacattgta ctccagtaac ggaggagtac
     3721 aggggctccc agttgtccag tacccttggg gtccctggca ggctgcacac aagccactca
     3781 agggcccctc acatgaagct gatgtcctcc ttgtgagaca agctgtcgtg acaagcaggg
     3841 catctctaaa caggctggag ggcttcacca agccctggga cctctgtggg ttcatttttt
     3901 tttgcttttt aaaccatcta ttggtttttg tctaaaaatc aataatgtga tggatttgag
     3961 ggattttctt tctgtagctg aaggtggaag gagtttattt cattagttaa ccaaatactg
     4021 agaggaaatt aaaatatcat tactaccaaa gatttttctc aataaaagct tctattttgg
     4081 taacacacct atattttcac tcgtggggat gagaccctca gcacttacca ttgtaatagc
     4141 ttctaaaaca agcctcatgg gccagcttca tttgatgtgc tagccagctg tacaaggcct
     4201 ctcggcacca ttgccagaag tgtctctctc gcccaagccg aagaaggcaa ttccctgcac
     4261 tcgtcttagg gcaaggcaga cagtgctgta gttgtgagct ggggtccgaa tctcaggctt
     4321 ccaaggtttg cactgtttgc taaaatcgtg agacctgatg cttctatttt ctcttgtgca
     4381 gtgatgtggt ttccaatgat gttttgtata tagctactgt atccaaaatt agataattac
     4441 caaaaattct tttacagaaa ccatttttaa aaaacaacaa aaaaaactaa aaaaaaaaca
     4501 aaaaaacaaa acaaaactga atgtatacac acccacagga gactgtggct gagcgttcat
     4561 ccaaataaat ttgaatacac aa
//
