LOCUS       NM_028085               2048 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ankyrin repeat and sterile alpha motif domain
            containing 4B (Anks4b), mRNA.
ACCESSION   NM_028085
VERSION     NM_028085.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2048)
  AUTHORS   Crawley,S.W., Weck,M.L., Grega-Larson,N.E., Shifrin,D.A. Jr. and
            Tyska,M.J.
  TITLE     ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation
  JOURNAL   Dev Cell 36 (2), 190-200 (2016)
   PUBMED   26812018
REFERENCE   4  (bases 1 to 2048)
  AUTHORS   Sato,Y., Hatta,M., Karim,M.F., Sawa,T., Wei,F.Y., Sato,S.,
            Magnuson,M.A., Gonzalez,F.J., Tomizawa,K., Akaike,T., Yoshizawa,T.
            and Yamagata,K.
  TITLE     Anks4b, a novel target of HNF4alpha protein, interacts with GRP78
            protein and regulates endoplasmic reticulum stress-induced
            apoptosis in pancreatic beta-cells
  JOURNAL   J Biol Chem 287 (27), 23236-23245 (2012)
   PUBMED   22589549
  REMARK    GeneRIF: Anks4b is a HNF4alpha target gene that regulates
            endoplasmic reticulum stress in beta-cells by interacting with
            GRP78, thus suggesting that HNF4alpha is involved in maintenance of
            the ER.
REFERENCE   5  (bases 1 to 2048)
  AUTHORS   Pettitt,S.J., Liang,Q., Rairdan,X.Y., Moran,J.L., Prosser,H.M.,
            Beier,D.R., Lloyd,K.C., Bradley,A. and Skarnes,W.C.
  TITLE     Agouti C57BL/6N embryonic stem cells for mouse genetic resources
  JOURNAL   Nat Methods 6 (7), 493-495 (2009)
   PUBMED   19525957
REFERENCE   6  (bases 1 to 2048)
  AUTHORS   Johnston,A.M., Naselli,G., Niwa,H., Brodnicki,T., Harrison,L.C. and
            Gonez,L.J.
  TITLE     Harp (harmonin-interacting, ankyrin repeat-containing protein), a
            novel protein that interacts with harmonin in epithelial tissues
  JOURNAL   Genes Cells 9 (10), 967-982 (2004)
   PUBMED   15461667
  REMARK    GeneRIF: Harp + harmonin constitute a scaffolding complex to
            facilitate signal transduction in epithelia.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY106969.1, AK008229.1, BC118013.1 and AI956809.1.
            
            On Apr 29, 2009 this sequence version replaced NM_028085.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK008229.1, AF524030.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BY106969.1         2-19
            19-1031             AK008229.1         1-1013
            1032-1412           BC118013.1         993-1373
            1413-2028           AK008229.1         1395-2010
            2029-2048           AI956809.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 64.45 cM"
     gene            1..2048
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /note="ankyrin repeat and sterile alpha motif domain
                     containing 4B"
                     /db_xref="GeneID:72074"
                     /db_xref="MGI:MGI:1919324"
     exon            1..225
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1333
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /note="harmonin-interacting ankyrin-repeat containing;
                     harmonin-interacting ankyrin repeat-containing protein"
                     /codon_start=1
                     /product="ankyrin repeat and SAM domain-containing protein
                     4B"
                     /protein_id="NP_082361.2"
                     /db_xref="CCDS:CCDS21793.1"
                     /db_xref="GeneID:72074"
                     /db_xref="MGI:MGI:1919324"
                     /translation="MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGN
                     LEALEIICSRGGDPDKCDIWGNTPLHYAASNGHTHCISFLVNFGANIFALDNDLKSPL
                     DAAASREQKECVALLDKAATVQNTMNPKRVTRLKEQALKNARKQMKECERLQERHQNK
                     MARTYSKEDSGTISSSHSTLSRSFLSTTSAGRFGSLSKGIKDTFKIKSKKNKDTTEQL
                     EKDGRSGQRPVMEVFREEEEDSFPKDFKEKLHFSVEEDDDVQHESILNRPGLGSIVFS
                     RNRVLDFEDISDSKRELGFKMPSELFQRQGAAGTVEEEEEEEEEEEEEKREANGTAGD
                     LPWDEEEVEWEEDAVDATPLEVFLQSQHLEEFLPIFMREQIDLEALLLCSDEDLQNIH
                     MQLGPRKKVLSAIDKRKQVLQQPGQLVDTSL"
     exon            226..2030
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2007..2012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /note="hexamer: AATAAA"
     polyA_site      2030
                     /gene="Anks4b"
                     /gene_synonym="2010013E14Rik; Harp"
                     /note="major polyA site"
ORIGIN      
        1 gtgttaccag ctctgcctag acaaagactc ctacctggcc aagctctggt gggcagaaaa
       61 aatgtctacc cgctatcacc aagcagcgag tgacagctac ctggaacttc tgaaggaggc
      121 caccaagcgt gatctgaatc tctcagatga ggatggcatg acacccactc tcctggcagc
      181 ctaccacggc aacctcgagg ccctagaaat aatctgcagt agaggtggag accctgataa
      241 gtgtgacatc tgggggaaca ctcctctaca ttatgcagcc tccaatggcc acactcattg
      301 catctcattt ctagtcaatt ttggtgccaa catctttgcc ctggataatg acttgaagtc
      361 tccactggat gctgctgcta gtagggagca gaaagaatgt gttgctctcc tggacaaggc
      421 tgccactgtg cagaacacca tgaaccccaa aagagtcacc agactgaagg aacaggctct
      481 gaagaatgcc aggaagcaga tgaaagagtg cgaacggctt caggagaggc accaaaacaa
      541 gatggcgcgc acctatagca aggaggactc agggacgatt tcttcttccc atagtacctt
      601 gtctaggtcg tttctttcaa ctacttcagc tggtagattt ggatcactgt ccaaaggcat
      661 taaagatacc ttcaagataa aatctaagaa gaacaaagat acaacagaac agctagagaa
      721 ggacggaaga agtgggcaga ggcctgtgat ggaagtgttc agagaggagg aagaagactc
      781 atttccaaag gacttcaaag agaagctcca tttctcagta gaagaagatg atgatgtaca
      841 acatgagtct attctcaacc gtccgggtct aggaagtatt gtttttagta gaaacagagt
      901 actggacttt gaggacatct ctgacagcaa gagggagttg ggttttaaaa tgcccagtga
      961 gctattccaa agacaaggag cagcaggaac agttgaagaa gaagaagagg aggaggagga
     1021 ggaagaggag gagaagagag aggcaaacgg cactgcaggt gatcttcctt gggatgaaga
     1081 agaagtagaa tgggaggaag atgcagttga tgctactcca ctggaagtgt tcttgcagtc
     1141 tcagcacctg gaggaattcc ttcccatttt tatgagagaa cagattgatc tagaggctct
     1201 gctgctttgc tctgatgagg accttcagaa tatccacatg cagctgggcc ccaggaagaa
     1261 agtccttagt gccatagaca aaaggaagca ggtgctccaa cagcctgggc agttggttga
     1321 caccagcctc taatggagga cattgattaa caggctagcc agagcctggg gttcatgctg
     1381 agttctcctg gaaccacccc aggatgaacc ctgtgcttgg tctctgtcta atgacaaacc
     1441 actgggtgta ggtgttaggg ttttagagag gacactccaa atcagaggct catactctgt
     1501 atccaagtaa accatcaatg agaaaagacc ttgggagacc ttgaaatggg aaaatggtgt
     1561 ttctcttcca ggctttgaat tctgtgtaga ccaaaggctc tcactagtct gaagcccaaa
     1621 gcccaactta atggatcatt taacagaagc ttgctaactg tggggttggg ttgacagtat
     1681 tcctgtctga gaatcaggac gtgtagcagg tagggggatg gcaggcagaa aatgtggact
     1741 gaatttctaa agttcatgta tggaagccct aacccctatg tgaatggttt tggagatagg
     1801 actttaaaga tgtgattaag gttgaatgag gttataaggg cagctcgtaa tccatatgac
     1861 ttaaaagaca aggaagagtg ggaagggttg gttcacactc aagaaaggag gacagcagga
     1921 aggcagctgc caaccagcca aggagagagg cactgagaat tcgaacctgg aaacagtttg
     1981 ggctttcagc ctaccgagat gcgaaaaata aatgtctgtt gtttgggcca aaaaaaaaaa
     2041 aaaaaaaa
//
