LOCUS       NM_145633               4292 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus ankyrin repeat domain 27 (Ankrd27), transcript variant
            1, mRNA.
ACCESSION   NM_145633 XM_911790
VERSION     NM_145633.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 4292)
  AUTHORS   Marubashi,S., Ohbayashi,N. and Fukuda,M.
  TITLE     A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through
            Stabilization of Varp Protein in Mouse Melanocytes
  JOURNAL   J Invest Dermatol 136 (8), 1672-1680 (2016)
   PUBMED   27066885
  REMARK    GeneRIF: RACK1 competes with Rab40C for binding to the ANKR2 domain
            of Varp and regulates dendrite outgrowth through stabilization of
            Varp in mouse melanocytes
REFERENCE   4  (bases 1 to 4292)
  AUTHORS   Yatsu,A., Shimada,H., Ohbayashi,N. and Fukuda,M.
  TITLE     Rab40C is a novel Varp-binding protein that promotes proteasomal
            degradation of Varp in melanocytes
  JOURNAL   Biol Open 4 (3), 267-275 (2015)
   PUBMED   25661869
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4292)
  AUTHORS   Ohbayashi,N., Yatsu,A., Tamura,K. and Fukuda,M.
  TITLE     The Rab21-GEF activity of Varp, but not its Rab32/38 effector
            function, is required for dendrite formation in melanocytes
  JOURNAL   Mol Biol Cell 23 (4), 669-678 (2012)
   PUBMED   22171327
REFERENCE   6  (bases 1 to 4292)
  AUTHORS   Tamura,K., Ohbayashi,N., Maruta,Y., Kanno,E., Itoh,T. and Fukuda,M.
  TITLE     Varp is a novel Rab32/38-binding protein that regulates Tyrp1
            trafficking in melanocytes
  JOURNAL   Mol Biol Cell 20 (12), 2900-2908 (2009)
   PUBMED   19403694
  REMARK    GeneRIF: Varp functions as the Rab32/38 effector that controls
            trafficking of Tyrp1 in melanocytes.
REFERENCE   7  (bases 1 to 4292)
  AUTHORS   Wang,F., Zhang,H., Zhang,X., Wang,Y., Ren,F., Zhang,X., Zhai,Y. and
            Chang,Z.
  TITLE     Varp interacts with Rab38 and functions as its potential effector
  JOURNAL   Biochem Biophys Res Commun 372 (1), 162-167 (2008)
   PUBMED   18477474
REFERENCE   8  (bases 1 to 4292)
  AUTHORS   Zhang,X., He,X., Fu,X.Y. and Chang,Z.
  TITLE     Varp is a Rab21 guanine nucleotide exchange factor and regulates
            endosome dynamics
  JOURNAL   J Cell Sci 119 (Pt 6), 1053-1062 (2006)
   PUBMED   16525121
  REMARK    GeneRIF: Varp is a potential Rab21 guanine nucleotide exchange
            factor and might regulate endosome dynamics in vivo
REFERENCE   9  (bases 1 to 4292)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
REFERENCE   10 (bases 1 to 4292)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY070464.1, BC065093.1, AK144733.1 and BY376293.1.
            
            On Feb 16, 2007 this sequence version replaced NM_145633.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.8080.1,
                                           SRR1660811.179027.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BY070464.1         2-31
            31-2159             BC065093.1         1-2129
            2160-3848           AK144733.1         2146-3834
            3849-4279           AK144733.1         3836-4266
            4280-4292           BY376293.1         439-451
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 21.37 cM"
     gene            1..4292
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /note="ankyrin repeat domain 27"
                     /db_xref="GeneID:245886"
                     /db_xref="MGI:MGI:2444103"
     exon            1..123
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            124..254
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..3299
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /note="isoform 1 is encoded by transcript variant 1;
                     VPS9-ankyrin repeat-containing protein; ankyrin repeat
                     domain-containing protein 27; VPS9-ankyrin-repeat protein;
                     mFLJ00040 protein; VPS9 domain-containing protein; ankyrin
                     repeat domain 27 (VPS9 domain)"
                     /codon_start=1
                     /product="ankyrin repeat domain-containing protein 27
                     isoform 1"
                     /protein_id="NP_663608.3"
                     /db_xref="CCDS:CCDS21154.1"
                     /db_xref="GeneID:245886"
                     /db_xref="MGI:MGI:2444103"
                     /translation="MALYDEDLLKNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLP
                     GSVQASCQFESYVLVPTEGHFQTLDGKAVVIEGNRIKLGAGFACLLSVPILFEETFYN
                     EKEESFSILCIAHPLERRETSEEPSAPADPFSLKTIEDVREFLGRHSEKFDKNIASFH
                     RTFRECERKSLRHHIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHH
                     DIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNK
                     CTSPQQKLLCLRKVVQLMTQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSY
                     IKNFRFSSSAKDELGYCLTSVEAAIEYIRQGSLSTKTPDAEGFGDRLFLKQRMNLLSQ
                     MTSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLISGRLND
                     PSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGF
                     QSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGD
                     TALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRR
                     PRPSEVPAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREVEKLLRAVADGDL
                     EMVRYLLEWTEDDLDDVEDAISTVDLEFCHPLCQCPKCAPAQKLARISANGLSVNVTN
                     QDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDS
                     NAKPNKKDLSGNTPLICACSAGHHEVAALLLQHGASINACNNKGNTALHEAVMGRHTL
                     VVELLLFYGASVDILNKRQYTAADCAEQDSKIMELLQVVPGCVASLDSVEEADHEGYV
                     TVEIRRKWNPKMYNLPEEPLRRQFCLINPGGRFQERTSRETMGRDRSVPDLAGRSLQE
                     PEKQRVTGKQSDLSDLSRYQTSEEGNKGLPERPVSRQAAPGHRPMVRRHTVNDAAILQ
                     VPEVTVHLTTHEASVPQS"
     exon            255..365
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            366..522
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            523..677
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            678..737
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            738..791
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            792..857
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            858..934
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1056
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1135
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1268
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1361
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1489
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1572
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1688
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1781
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1979
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2039
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2159
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2204
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2324
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2522
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2642
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2804
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2916
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2917..2995
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3068
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..4292
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4262..4267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /note="hexamer: AGTAAA"
     polyA_site      4280
                     /gene="Ankrd27"
                     /gene_synonym="D330003H11Rik; Varp"
                     /note="major polyA site"
ORIGIN      
        1 ggcggagtaa agaggtaggc tgaagagtgg agccaccgca cccgggtggt gggcactcag
       61 gtcccagccc agggtccggg aggtgctgtc gtagcacaga ggtgaaacta tgtagcctct
      121 taagaagctc acttggatcc cccgaagtcc ctatggctct atatgatgaa gatctcctga
      181 aaaacccttt ctacctggcc cttcagaagt ggcgccccga cttgtgcagc aaggtggccc
      241 agatccatgg cattgtccta gtgccttgca gaggaagcct gcccggcagt gtgcaggcct
      301 cctgccagtt cgagtcctat gttttggtac ccacggaagg acactttcag accttagatg
      361 gaaaggctgt cgtcattgaa ggaaacagga ttaagctagg agcaggtttt gcttgccttc
      421 tctctgtgcc catcctcttt gaggagactt tctacaacga gaaagaggag agtttcagca
      481 ttctctgcat tgctcatcct ttggagagga gagagacttc agaagaacct tcagcgcctg
      541 cagatccttt ctccctgaaa accatcgaag atgtgagaga atttttggga agacactcag
      601 agaaatttga caagaacatt gcctctttcc accggacgtt ccgagaatgt gaaagaaaga
      661 gcctccgcca ccacatagac tccgtaaatg ctctctacac caaatgcctc cagcagcttc
      721 tccgagactc tcacctgaag gttcttgcaa agcaggaggc ccagatgaac ctgatgaagc
      781 aggccgtaga gatgtatgtc catcacgata tttacgacct gatctttaaa tacgtgggga
      841 ccatggaggc gagcgaggat gccgccttta acaaaatcac gagaagcctt caagatctcc
      901 agcagaaaga catcggcgtg aaacccgagt tcagcttcaa catccctcgg gcaaagagag
      961 agctgggtca gttgaacaag tgtacgtcgc cacagcagaa gctgctgtgc ctgaggaagg
     1021 tggtccagct catgacacaa tctcccagcc agagagtgaa cttggagacc atgtgtgccg
     1081 atgatctcct ctcggtcctg ttatatctgc tcgtgaagac agagatccct aactggatgg
     1141 caaatttgag ctacatcaaa aacttcagat ttagcagctc ggccaaagat gagctaggat
     1201 actgcctgac ctcagtcgag gctgcgattg aatacatccg gcaaggaagt ctctccacga
     1261 agacgcctga cgctgaggga tttggcgaca gactatttct caagcagcgg atgaacttgc
     1321 tgtcccagat gacatcaaca cccatcgact gcctgttcaa gcacatcgcc tctggtaacc
     1381 aaaaggaagt ggaaagactt ctgagccaag atgaccagga caaagacgcc atgcaaaaga
     1441 tgtgccaccc actttgttcc tgtgaggact gtgagaagct tatctctggg aggctgaatg
     1501 acccgtcagt tgtcactcct ttctccagag atgacagagg tcaaacaccc ctccatgtgg
     1561 ctgccctctg tggtcaggca tccctcattg acttcctggt ctccaaaggt gccgtagtga
     1621 acgccacaga ctatcatggg tccaccccct tacatctggc atgtcagaaa ggcttccaaa
     1681 gtgtgacgct gctgctgctg cactacaagg ccagcactga ggttcaggac aacaacggca
     1741 acaccccgct gcacctggcc tgcacctatg ggcaggagga ttgcgtgaag gccctggtct
     1801 actatgatgt ccaggcctgc agattggata ttggtaatga gaaaggagac acagccctgc
     1861 acattgctgc acgctggggt tacgagggca tcatagagac actgctgcag aatggagccc
     1921 ccacagcagt ccagaacaga ctgaaggaaa caccactcaa gtgtgctctg aactcgaaga
     1981 ttctgtctat catggaagct catcacctat cctctgacag gaggccaagg ccttctgagg
     2041 ttcctgcaca gtcccctaca cgctctgtgg attccatcag ccagggctcc tccacctcca
     2101 gcttttcttc catatcggtg agcttcaggc aagaagaggt caaaaaggac tacagggagg
     2161 tagaaaagct tctaagggca gttgctgatg gcgatctaga aatggtacga taccttttgg
     2221 agtggacaga ggatgaccta gacgatgtgg aagacgccat cagcacggtg gatctcgaat
     2281 tctgtcaccc cttatgccag tgtcccaagt gtgctccagc tcagaaactg gccaggattt
     2341 ctgccaatgg gcttagtgtg aatgtgacca accaggacgg cttctctccc ctgcacatgg
     2401 ctgccctgca tggccgcaca gacctcgtcc ccctcctgct gaagcacggt gcttactcag
     2461 gtgccagaaa cacaagccaa gccgtcccgc tccacctggc ctgccagcag gggcactttc
     2521 aggtggcaaa atgtctctta gattccaatg caaaacctaa taaaaaggac ctcagtggga
     2581 acacacccct catttgtgcc tgctctgccg gccaccatga agtcgctgcg ctgttgctac
     2641 agcacggggc ctccatcaat gcttgcaaca acaaaggcaa cacggccctg catgaggctg
     2701 tgatgggaag gcacaccctg gtagtggagc tgcttctgtt ctacggggcg tcggttgaca
     2761 tcctgaacaa gaggcagtac acggcggcgg actgtgccga gcaggactca aaaataatgg
     2821 agttgctgca ggtagtgcca ggctgtgttg cgtctctaga cagcgtcgag gaggcagacc
     2881 acgagggtta cgtgactgtt gagatcagga gaaaatggaa ccccaaaatg tacaacctgc
     2941 cagaggaacc tcttaggaga cagttttgcc tcattaaccc ggggggtcgg ttccaggaga
     3001 ggacatccag ggagactatg ggaagggata gaagtgtgcc cgatcttgca ggacgttctc
     3061 ttcaggagcc agagaagcaa agagtcaccg gaaagcagag tgacctgtca gacctgagca
     3121 ggtatcagac ctctgaggaa ggaaacaagg ggctcccgga gaggcccgtg tcgagacagg
     3181 ctgctccagg acaccggccg atggtccgca gacacacagt caatgacgca gccatactcc
     3241 aggtcccaga ggtgactgtc cacctcacca cccacgaggc gagtgttcct cagtcttagc
     3301 ggtaagggcc tggtaaatct acggagacac gagtccacgg taagatggtg agcatgaaca
     3361 cagtatagaa ctaagcatac tttcaataac cagcttgaga gacagcccct cggacttacc
     3421 accctctcct cttcaaagct aactcacata cttgaaaagg aatgggaggg actgaccgag
     3481 cccctccatc gcagcattcg ggtttcctgg tcacaggcag agacttcatc gccaggtctg
     3541 acctcctttg gctggaaggt gctaagtgtc tgtgaggtgg ataagtaggc aagccttacg
     3601 ctagaaggat gcccatgtcc aggagatgtc agagaagcaa tgccggtctt ccggaaccgt
     3661 ggataccaag cagagaagca tcccattggg atgaagaaac ggaacctgga aacacttgaa
     3721 ctggaagata cctgaatgct gttttgacgt ccgtcccagt gaaaaggacc taccattctg
     3781 ccttgttcta caccatgtgt ctggggaaag ttggccgtcg ctttcccaac aacacagacc
     3841 cccctgggtc gctgcacgtt tgcctgtatg aatttctgct cactctcctt tccgttagag
     3901 cctccccagc acagcacacc tgacagacgg ttactagaga gcagctttgg cacatcttaa
     3961 gtctttcgat tcaaattcaa aacttagagc acaaaccagg tcagagttta agttaggatt
     4021 tgttgccatt gattcctttt tataactttc tatagattct attatttttt tatgttcctg
     4081 accctaaagt tgcagacatg gattcagagg gttttgtaaa ggggaaatgg ttttgtgcac
     4141 gctattggaa atacttgtga atgtatagaa gggaggggct gattattttt ctacaaagta
     4201 atttatgatt tctaattttc taatgtgcct tggatatgtg ccaaatgatg gaaaagaaaa
     4261 cagtaaactt tgcctctcga ttgtgtcgtg cc
//
