LOCUS       NM_001253814            5259 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2),
            transcript variant 1, mRNA.
ACCESSION   NM_001253814
VERSION     NM_001253814.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Han,B.Y., Wu,S., Foo,C.S., Horton,R.M., Jenne,C.N., Watson,S.R.,
            Whittle,B., Goodnow,C.C. and Cyster,J.G.
  TITLE     Zinc finger protein Zfp335 is required for the formation of the
            naive T cell compartment
  JOURNAL   Elife 3, e03549 (2014)
   PUBMED   25343476
  REMARK    GeneRIF: Restoring the expression of one Zfp335 target, Ankle2,
            partially rescues T cell maturation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5259)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5259)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   5  (bases 1 to 5259)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 5259)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132187.1, AC118260.7, BC065071.1 and CD538245.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132187.1, SRR10223971.6049.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-477               AK132187.1         3-479
            478-478             AC118260.7         105561-105561
            479-4077            AK132187.1         481-4079
            4078-5256           BC065071.1         4038-5216
            5257-5259           CD538245.1         1-3                 c
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.4 cM"
     gene            1..5259
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="ankyrin repeat and LEM domain containing 2"
                     /db_xref="GeneID:71782"
                     /db_xref="MGI:MGI:1261856"
     exon            1..420
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..3128
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ankyrin repeat and LEM domain-containing protein 2; LEM
                     domain-containing protein 4"
                     /codon_start=1
                     /product="ankyrin repeat and LEM domain-containing protein
                     2 isoform 1"
                     /protein_id="NP_001240743.1"
                     /db_xref="CCDS:CCDS57372.1"
                     /db_xref="GeneID:71782"
                     /db_xref="MGI:MGI:1261856"
                     /translation="MLWQRLAVVEWAALAWELLGASVLFIAVRWLVRRLEKRPRDLNR
                     CGTLSSPPSASEAVAAQPGEVTMDAMMARLKLLNPDDLRKEVMKAGLKCGPITSTTRF
                     IFEKKLAQALLEQGGLLTSSLPKPSAVTAMAFIQGTSRTPPSVDGKQTQQACFSEDRD
                     FGYSVGLNPPEEEAVASSVHPVPFSASTRNDNHKAGVTAPKEPLVYYGVCPVYEDGPV
                     RHERIHVYEDKKEALQAAKLIKGSRFKAFRTREDAEKFARGICDYLPSPNKTTPLLSP
                     VKAVPLGGSDGLKADGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVENGEEHTFS
                     DLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASMCQLTLETLENPEFMRLMY
                     PDDNMDMLQKRILYVVDLYLNTPDKVGFDTPLHFACKFGNVDVVNVLSSHPLIVKNRK
                     NKYGKTPEEVICERSQNKSPALKERIREYLMGHYYVPLLRAEDTSPVIGELWSSDQKA
                     EASNTAHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGFFHSIRKSDPE
                     RGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELSKKAQQEIR
                     ENEGCLQDRTSDFGSGKKYSNSISVGAFLDGDDDSSLEEIKNQQNTVPSQSQPTTDKF
                     QTSKSGSLPLGQKVDPGETSVGTYPDKGRNGFCHPLNHRTADGRGLEATNGEEALPPP
                     VSVLTQELNKLNLQSLGDSLHETPDKNGKLEDEVLPSRKGAADSDLLASPPAIASLGK
                     KQVRTNTEVSEAMAEMSLGPKSPQLGVQAGLEPILSSATVDSTKRLFLSGEEPSKLDR
                     DVLAALECANIDPGLYPAIHRWKSTVMCYSPSDRQSWPSPALKGKFTTELVDLDCSHS
                     CSGRCSPAGSSPSKPGHTSSSSGLHSPGRYSPAHGRHFQRVAHVARLAAL"
     exon            421..894
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1101
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1298
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1487
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1610
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1677
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1847
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..1954
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2145
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2776
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2908
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     exon            2909..5259
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3258..3263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="hexamer: AATACA"
     polyA_site      3278
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="major polyA site"
     regulatory      4552..4557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="hexamer: AATAAA"
     polyA_site      4574
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
     regulatory      5234..5239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
                     /note="hexamer: ATTAAA"
     polyA_site      5256
                     /gene="Ankle2"
                     /gene_synonym="1110001J12Rik; D5Ertd585e"
ORIGIN      
        1 ggagccgcct gaggctgcgt tccgggccgc gttctagcct gatcgttggg aaacgggacg
       61 gcccgcactg cgcgcgcagc ccgcctggtc taaccacggc cgggcggagg aggaggcttg
      121 gggcaggcgc gactcccgtg gcgaggcgag cggtcttctg agggccggag gcgggagcga
      181 cgcgggctgc atttgcagcg gccgcgctca aggcctcggc gccggcggtg gcgatgctgt
      241 ggcagcggct ggctgtggtg gagtgggcgg ccctggcctg ggagctcctg ggcgcctcgg
      301 tgctgttcat cgccgtgcgc tggctggtcc gccggctaga gaagcggccg cgggacctga
      361 accgttgcgg gacgctctcc tctccgccca gcgcgtctga agcggtggcc gcacagccag
      421 gtgaagtgac aatggatgcc atgatggctc gactgaaact cttgaatcca gatgacctta
      481 gaaaagaagt tatgaaagct ggacttaaat gtggacccat tacatcaaca acaagattta
      541 tttttgagaa gaaattagct caggccttac tggaacaagg tggattgctg acctcttccc
      601 ttcccaagcc cagcgcggtt actgccatgg catttatcca gggcacctca aggactcctc
      661 catctgtgga cgggaaacaa actcagcagg cctgtttttc tgaagacaga gattttggtt
      721 acagtgtggg cttgaaccct ccagaagagg aagcggtagc atccagtgtc cacccagtac
      781 ccttcagtgc ctctaccagg aatgacaacc acaaggctgg agtgacagct cctaaggagc
      841 cacttgtgta ctatggagtg tgtccagtat atgaggatgg ccctgtgaga catgagagga
      901 tccatgttta cgaagataaa aaggaagctt tacaagctgc caagttgatc aaagggtccc
      961 ggtttaaagc ttttcgaaca agagaagatg ctgagaaatt tgctagaggg atatgtgatt
     1021 atttaccttc tcccaacaaa actacgccct tactgtctcc tgtgaaggca gtgcccctcg
     1081 gtggcagtga tgggctgaaa gcagatggct tgtgcctggc tgaatcagaa acagtaaaca
     1141 aagaacgagc aaacagttac aaaaaccccc gtacccagga cctcacggcc aagctgcgga
     1201 aagctgtcga aaacggggag gaacacacct tttctgatct tatctggagt aatcctcgat
     1261 atctaattgg ttctggggac aacccaacca ttgtacaaga aggatgtagg tacaatgtca
     1321 tgcatgttgc tgccaaagag aaccaggctt ccatgtgcca gctgacatta gagactctgg
     1381 agaaccctga gtttatgcgt ctgatgtacc cagatgacaa catggacatg ctacagaagc
     1441 gcatcctcta cgttgttgac ctgtatctta atactcctga caaagtgggc tttgatacac
     1501 cactgcattt tgcttgtaaa tttggaaatg tggatgtagt caatgtgctt tcttcacacc
     1561 ctttgattgt gaaaaaccga aagaataaat atggtaaaac acctgaagaa gtaatttgtg
     1621 aaagaagcca aaacaaatct ccagcactga aagaaaggat tagagaatac ttaatgggtc
     1681 actactatgt gccactcctg agagcagaag acacttctcc tgtcattggg gagctgtggt
     1741 cctcagacca gaaagctgaa gcctcaaata ctgcccactg tagaagcagc cccagagatc
     1801 ctgtgatgac actgagagct ttcgtgggac ctctgagtcc atccaaagca gaagattttc
     1861 gaaagctctg gaaaactcca cctcgaaaga aagcaggctt cttccacagc atcagaaagt
     1921 ctgacccaga acgaggcatt gagagagttg gaagggagct agctcatgag ctggggtatc
     1981 cctgggttga atactgggaa tttctgggat gttttgttga tctgtcttcc caggaaggct
     2041 tgcaaagact agaagaatac cttatccaga aggagttaag caaaaaggct caacaagaaa
     2101 tacgagaaaa tgaaggctgc cttcaggaca gaacctcaga ttttggcagt ggaaagaagt
     2161 acagcaactc catctccgtg ggagcatttc tagatgggga tgatgacagt agtttggaag
     2221 aaattaaaaa ccagcaaaat acagttccaa gtcagagtca gcccacaact gataagttcc
     2281 aaacctctaa aagtggttcc cttcccttgg gacagaaggt cgaccctgga gaaacctcag
     2341 ttggaactta cccagataaa ggcagaaatg ggttttgtca ccctctaaac cacaggactg
     2401 cagatggaag gggactagag gccaccaatg gggaggaggc ccttccacca cctgtttctg
     2461 ttttgactca agagttgaat aaactgaatt tgcaaagcct gggagacagc cttcatgaga
     2521 caccagataa aaacgggaaa ctagaggatg aggtgcttcc ttcaagaaag ggtgcagcag
     2581 acagtgactt gctggcatct cctcctgcca tagcaagcct tggaaagaag caagtcagga
     2641 ccaacactga ggtgtcagag gcaatggctg aaatgtcttt gggccctaag agcccccaac
     2701 ttggagttca ggctggtctg gaacctatac tctcatctgc cacggtagat tccaccaaga
     2761 ggctcttcct gtctggagag gaaccatcaa aactagatcg agatgtttta gcagctcttg
     2821 agtgtgcgaa tattgaccct ggtctgtacc cagcaatcca cagatggaaa agcactgtca
     2881 tgtgctactc gccctcagac agacagagtt ggccaagtcc tgcactcaaa gggaagttca
     2941 caactgagtt ggtggatctc gattgctctc atagctgctc aggacgatgc agtccagctg
     3001 gtagcagccc cagtaagcct ggccacacct cctcctcttc tggcctgcac agccctggac
     3061 gctacagtcc agcacatggg agacacttcc aaagagtggc acacgtggcc cgacttgcag
     3121 ctctgtagag ccagcttact cctgggattc atttacttct ttgtttgtaa aagagtaatg
     3181 tttattggta aaaattccat gaaagaatgt atcctgaata acttaatcct cgctttgagg
     3241 gtactggtat tttagaaaat acaatgttct gagtataatt tgaatgtctg atattttgga
     3301 agaagttaat gaaagcaaac tcagatacta attgtttgaa agtgtagccg taataatgat
     3361 tcagactttt caggtggaaa cttctaattt tgaaatagaa caaagatgca aaggcataga
     3421 caagtgaggt gttgctaatt gggtttgtta gcagcaacac tgcagcacgt gcacacaccc
     3481 actatttctg aaacagatca attatgtatt gtctcttggg ggggtgcagc tttttgatat
     3541 gcaatggcaa aactatgtgt ttgtcatttc accctagtta ttccaaccga gagaaagcag
     3601 gtggcagaaa agcagatgtg ccctgactca gacttcagta acaatgctca aaacactgcc
     3661 agaagttggg atggggggtt aaagccatcg agaatcatgc gagtaatcat catagaaaac
     3721 tcaccagcaa taactaaaac caaaactatt tctcagtact tgtcagtgta gtaagttgtc
     3781 tagaagttgt acccaggctg gagaaatggc tcagcggtta agagcaccga ctgctcttcc
     3841 aatggtcctg agttcaattc ccagcaacca catggtggct cacaaccatc cataataaaa
     3901 tctgatgcct tcttttgggg tgtctgaaaa cagatacagt gtacttacat ataataaata
     3961 catcttttat aaaaaaaaga aattgttccc aagtcttaaa atttagcctg agatttcact
     4021 aagcatgatt acatgacaga tgtatgccgt attttagaaa ttaaagagct ttatgctaga
     4081 gtttagcact tgagatctga gttcatttca gtggcttgca gtgttgactt ttggctatcc
     4141 caagagatgt gtaccgagtg gcattgcaca ccctaaggct ccaagataaa accaccttgc
     4201 tgaagctggg tctcagctgt ctgtcagttg cttgcacaga gccagtagtg ttctggagac
     4261 acttgcagtg cttcacccca gtacagaact ataccactga ttttgccaat ttcagtgcct
     4321 tttgtgagtc attttgagat cacatagtgc taaatggggg cgttagattc tcttttgttt
     4381 ataaaaatgt tgaccaggca agcctttaag cccagcactc attaggcaga agcagggggt
     4441 tctctgaatt tgaggccagc ctggtctaga tagtgagttc caagacagcc agaactacac
     4501 agaggatctt gtctcaaatt taaaacaaaa acaaaaaccc accaaaatga taataaagta
     4561 attctgagct caaatgttta ggtcttttct tttttctttt tttttttttt taactttttt
     4621 aaagcttatt ttaatgtggt aagaaatacc agaattgaga ttttggactt tggatttagt
     4681 ttgttttgtt ccccttgtaa gcagctgagc tctaagtaca agacagccaa tcaagtgacc
     4741 tggaaacaat cccaaaggga atctttgttc tcaaaagaaa ccaaaaccca cacactatat
     4801 tcagctatga ttttgttttc tctaaagtct tattgtcctg tgcggttatt tttgttgttt
     4861 tttccatact caaaaactga agtactagtt ttgccctatt ttaacttttt tttttttaaa
     4921 ctcttctttc ttgatattta cttaagacaa cttttgggag tcagttctca aaccatgtag
     4981 gtctcaggaa tccaactcca gtcactggaa ttgagtttta cccgagccat cttgctcgcc
     5041 cattttaaac cctaagctgc ctttggacta tttttccact agtatgaagg gtatatacct
     5101 gttagtaaca cctgttgatc tatgtcataa acattgttta cttgatcttt cgcaaagtgt
     5161 tgggtaggaa acgactcctt aatttgttat tggacagaat ccccttctta gtaaaactgt
     5221 aagaaatatt ttcattaaaa aggacattga gtagcacat
//
