LOCUS       NM_172756               2772 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ankyrin repeat and LEM domain containing 1 (Ankle1),
            transcript variant 1, mRNA.
ACCESSION   NM_172756 XM_001478168 XM_001478177 XM_001478396 XM_001478407
VERSION     NM_172756.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Braun,J., Meixner,A., Brachner,A. and Foisner,R.
  TITLE     The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM
            Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse
            Hematopoiesis
  JOURNAL   PLoS One 11 (3), e0152278 (2016)
   PUBMED   27010503
  REMARK    GeneRIF: lack of Ankle1 does neither affect mouse hematopoiesis nor
            DNA damage repair in mouse embryonic fibroblasts, indicating a
            redundant or non-essential function of ANKLE1 in mouse
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ046325.1, EU184014.1, BC094316.1 and AK083552.1.
            
            On Jul 15, 2015 this sequence version replaced NM_172756.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094316.1, EU184014.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                CJ046325.1         2-25
            25-1629             EU184014.1         1-1605
            1630-2134           BC094316.1         1587-2091
            2135-2772           AK083552.1         2434-3071
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.43 cM"
     gene            1..2772
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /note="ankyrin repeat and LEM domain containing 1"
                     /db_xref="GeneID:234396"
                     /db_xref="MGI:MGI:1918775"
     exon            1..86
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1629
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ankyrin repeat domain 41; LEM-domain containing 3;
                     LEM-domain containing protein 3; ankyrin repeat
                     domain-containing protein 41; ankyrin repeat and LEM
                     domain-containing protein 1"
                     /codon_start=1
                     /product="ankyrin repeat and LEM domain-containing protein
                     1 isoform 1"
                     /protein_id="NP_766344.2"
                     /db_xref="CCDS:CCDS52583.1"
                     /db_xref="GeneID:234396"
                     /db_xref="MGI:MGI:1918775"
                     /translation="MADTACLALRLLAALREEEARAVEELLRLGADPNLVLDDGAAAV
                     HLAARASHPRALHCLRMLLRWGADPNARSAEGLTPVHVAAAWGCCGALELLLSRGGDP
                     TLRDQDGLRPLDWALQQRHHNCARVLQELDTPTQPDETREPTETFHVAQGSFETETCQ
                     GPALAESSGVSQDSELHVHRAELEVEAVEVAVHPQSSEATENSDYSSDASFVTAVEDS
                     LQPGRPGGALELVAGLWVTRGAVSAGKGAPNCQPQVLTLTARDTDKPVLPGDGDLGAL
                     HPHSSVPPMSDLQLLQALRALGYSPGPVTPFTRGHYLRRLQEAQASRADVGHSQELAE
                     ALRTGTIPDCQVDEEALAQCFQRLDPLKKWREGITKSSFTYLLLDPRLTKDLPARASS
                     LTLAECLQCFVRAIFYVGKGTRARPDAHLWEAFGYHDQPRKQVCPKVRRILDIWASGR
                     GIISLHCFQHVVAMEAYTREACLLDALGLQTLTNQKQGHYYGVVAHWPPSRRRRLGVH
                     LLQRALLVFLAEGERELRPQDIQARG"
     exon            87..239
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            240..345
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            346..457
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            458..994
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1166
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1326
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1456
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..2772
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2109..2114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /note="hexamer: AACAAA"
     polyA_site      2139
                     /gene="Ankle1"
                     /gene_synonym="8430438L13Rik; Ankrd41; C430003I23; Lem3"
                     /note="major polyA site"
ORIGIN      
        1 agagggcggt gactcctatg ggacatggcc gatactgcat gcttggctct gaggctgcta
       61 gcagcactac gcgaggagga ggcgcgggct gtggaggagc tgctgcgcct cggagcggac
      121 cccaacctgg tgttggacga tggcgctgcg gcggtgcacc tggcagccag agcgagccac
      181 ccgcgcgccc tgcattgcct tcggatgctg ctgcgctggg gagcggatcc caatgctcgg
      241 tccgcggagg ggctgacgcc tgtgcatgtg gctgccgcgt ggggctgttg tggtgctctg
      301 gagttgctgc tgagccgagg aggtgacccc acactgcggg accaggatgg gctcaggccc
      361 ttggactggg cactacagca gcgacaccac aactgcgcac gagtgctgca ggagctggac
      421 acgcccaccc agcctgatga gacccgagaa cccacagaga cgtttcatgt agcccagggt
      481 agctttgaaa ctgaaacatg ccaaggacct gccttagcag agtcttctgg agtttctcaa
      541 gattcggagt tgcacgtgca tagagctgag ctggaggtgg aggctgtaga ggtggctgtg
      601 catccccaga gctctgaggc caccgagaac tcagactaca gcagcgatgc ttcgtttgtc
      661 actgcagtgg aggactctct gcagcccggg cgccctgggg gggcattgga gctagtggct
      721 ggcttgtggg taacccgtgg ggctgtgtca gctgggaagg gagcacccaa ctgccagccg
      781 caggtcctga cattgactgc aagggacacg gacaaacccg tactgcctgg tgatggggat
      841 ctgggggctt tgcaccccca cagttcggtg ccccccatgt cggatctgca gctcctccag
      901 gccctgcggg cgctgggcta tagccctgga cccgtcacac ctttcaccag aggacactat
      961 ctacggcggc tgcaggaagc ccaggctagc cgtgctgatg tagggcatag ccaagaactg
     1021 gcggaggcac taaggacagg caccatccct gactgccagg tggatgagga agccctggct
     1081 cagtgctttc agcggctgga tcccttgaag aagtggcggg aaggcattac aaagtctagt
     1141 tttacgtatc tacttctcga ccccaggctg acgaaggacc tgccggcccg agcctcttcc
     1201 ctcacactgg ctgagtgtct gcaatgtttc gtgagagcca tcttctatgt gggcaaggga
     1261 acccgagccc gaccagatgc ccacctctgg gaagcctttg gctaccatga ccagccaagg
     1321 aaacaggtct gtcccaaggt tcgccggatc ttagacatct gggctagtgg ccggggcatc
     1381 atctctctgc actgcttcca gcatgtggtg gccatggaag cgtacactcg ggaagcctgt
     1441 ctgttggatg ccctaggtct ccagacactg accaaccaga aacaagggca ctactatgga
     1501 gtggttgccc attggccacc ctctcggcgt cggcgtttgg gggtgcacct gctacagcgt
     1561 gctctgctgg tcttcctggc tgagggggag agagagctga ggcctcagga cattcaggct
     1621 cgaggctgaa tgctgaggat gtgacatgaa tcctgagcca tcaaggtgtc tgagcatctg
     1681 gctggttcct aactgttccc agctctagct tcagaggaca gtgtgttttc ttgtgtgtgt
     1741 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtatgtgt gtatgtgtgt
     1801 gtgcgtgcgc gcgtgtgtgt gcgtgtatgt gtgtgttttt aaatatgtga cagtatttct
     1861 taatcaagct gagtgaacat tgggatacat ggtgcttctg gaagagtagt gagctcccca
     1921 tcacgggagg aaaacaagcc aaggagcaga agctattctg gagcaccacc tttaatccca
     1981 gcattcagga ggcaatagca gacagagctc tgtgagttca aggacagcct ggtttataga
     2041 aatgagttcc agtgcagcca ggggatatac agagaaacca tgtcccccaa aacaaaccaa
     2101 aaaacaacaa caaagccaag atggtactct ggaatgctac tttgtctcca tgtctcatct
     2161 tctgatgcaa ggaagagctc aagagagata atccaggtgc actggtagtg atggttcatc
     2221 atccctgcct ttgggaagct gaggcaggag gattgctagt tggaggccag cctgggcacc
     2281 tcagaatggg aattgacatt tatgttagtg gtgacagtcc ttttccccat ggctatccat
     2341 actccagaca gcctgggatc cccactgtgc tcctaggagc gcatcttaga tcaaacagca
     2401 ctgaagagtt ccaaactgac tgtggggaag tggtgggaga gtgcacactc acatgtcgct
     2461 agtgtccttg cacaccgact gtggtgggag agtgcacact cacatgtcgc tagtgtcctt
     2521 gcacactgac tgtagtggaa gagtgcacac tcacatgtcg ctagtgtcct tgcacaccga
     2581 ctgtggtggg agagtgcaca ctcacatgtc gctaagtgtc cctgcacact gactgtagtg
     2641 gaagagtgca cactcacatg tcgctagtgt ccttgcacac cgactgtggt gggagagtgc
     2701 acactcacat gtcactagtg tccctgcact gtcttttgaa agatgaatca tttaatatag
     2761 tgtaagtagt gc
//
