LOCUS       NM_020332               3511 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus progressive ankylosis (Ank), mRNA.
ACCESSION   NM_020332
VERSION     NM_020332.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ohnishi,T., Tran,V., Sao,K., Ramteke,P., Querido,W., Barve,R.A.,
            van de Wetering,K. and Risbud,M.V.
  TITLE     Loss of function mutation in Ank causes aberrant mineralization and
            acquisition of osteoblast-like-phenotype by the cells of the
            intervertebral disc
  JOURNAL   Cell Death Dis 14 (7), 447 (2023)
   PUBMED   37468461
  REMARK    GeneRIF: Loss of function mutation in Ank causes aberrant
            mineralization and acquisition of osteoblast-like-phenotype by the
            cells of the intervertebral disc.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Szeri,F., Niaziorimi,F., Donnelly,S., Fariha,N., Tertyshnaia,M.,
            Patel,D., Lundkvist,S. and van de Wetering,K.
  TITLE     The Mineralization Regulator ANKH Mediates Cellular Efflux of ATP,
            Not Pyrophosphate
  JOURNAL   J Bone Miner Res 37 (5), 1024-1031 (2022)
   PUBMED   35147247
  REMARK    GeneRIF: The Mineralization Regulator ANKH Mediates Cellular Efflux
            of ATP, Not Pyrophosphate.
REFERENCE   3  (bases 1 to 3511)
  AUTHORS   Nagasaki,A., Nagasaki,K., Chu,E.Y., Kear,B.D., Tadesse,W.D.,
            Ferebee,S.E., Li,L., Foster,B.L. and Somerman,M.J.
  TITLE     Ablation of Pyrophosphate Regulators Promotes Periodontal
            Regeneration
  JOURNAL   J Dent Res 100 (6), 639-647 (2021)
   PUBMED   33356859
REFERENCE   4  (bases 1 to 3511)
  AUTHORS   Szeri,F., Lundkvist,S., Donnelly,S., Engelke,U.F.H., Rhee,K.,
            Williams,C.J., Sundberg,J.P., Wevers,R.A., Tomlinson,R.E.,
            Jansen,R.S. and van de Wetering,K.
  TITLE     The membrane protein ANKH is crucial for bone mechanical
            performance by mediating cellular export of citrate and ATP
  JOURNAL   PLoS Genet 16 (7), e1008884 (2020)
   PUBMED   32639996
  REMARK    GeneRIF: The membrane protein ANKH is crucial for bone mechanical
            performance by mediating cellular export of citrate and ATP.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3511)
  AUTHORS   Chu,E.Y., Vo,T.D., Chavez,M.B., Nagasaki,A., Mertz,E.L.,
            Nociti,F.H., Aitken,S.F., Kavanagh,D., Zimmerman,K., Li,X.,
            Stabach,P.R., Braddock,D.T., Millan,J.L., Foster,B.L. and
            Somerman,M.J.
  TITLE     Genetic and pharmacologic modulation of cementogenesis via
            pyrophosphate regulators
  JOURNAL   Bone 136, 115329 (2020)
   PUBMED   32224162
REFERENCE   6  (bases 1 to 3511)
  AUTHORS   Sampson,H.W., Davis,R.W. and Dufner,D.C.
  TITLE     Spondyloarthropathy in progressive ankylosis mice: ultrastructural
            features of the intervertebral disk
  JOURNAL   Acta Anat (Basel) 141 (1), 36-41 (1991)
   PUBMED   1659102
REFERENCE   7  (bases 1 to 3511)
  AUTHORS   Krug,H.E., Mahowald,M.L. and Clark,C.
  TITLE     Progressive ankylosis (ank/ank) in mice: an animal model of
            spondyloarthropathy. III. Proliferative spleen cell response to T
            cell mitogens
  JOURNAL   Clin Exp Immunol 78 (1), 97-101 (1989)
   PUBMED   2805429
REFERENCE   8  (bases 1 to 3511)
  AUTHORS   Mahowald,M.L., Krug,H. and Halverson,P.
  TITLE     Progressive ankylosis (ank/ank) in mice: an animal model of
            spondyloarthropathy. II. Light and electron microscopic findings
  JOURNAL   J Rheumatol 16 (1), 60-66 (1989)
   PUBMED   2541245
REFERENCE   9  (bases 1 to 3511)
  AUTHORS   Sampson,H.W.
  TITLE     Spondyloarthropathy in progressive ankylosis (ank/ank) mice:
            morphological features
  JOURNAL   Spine (Phila Pa 1976) 13 (6), 645-649 (1988)
   PUBMED   3175755
REFERENCE   10 (bases 1 to 3511)
  AUTHORS   Hakim,F.T., Brown,K.S. and Oppenheim,J.J.
  TITLE     Hereditary joint disorder in progressive ankylosis (ank/ank) mice.
            II. Effect of high-dose hydrocortisone treatment on inflammation
            and intraarticular calcium hydroxyapatite deposits
  JOURNAL   Arthritis Rheum 29 (1), 114-123 (1986)
   PUBMED   3004514
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK083135.1, BC054379.1 and AW049351.1.
            
            On Oct 6, 2007 this sequence version replaced NM_020332.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ832285.1, AK083135.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                AK083135.1         2-94
            94-3287             BC054379.1         1-3194
            3288-3511           AW049351.1         1-224               c
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 10.23 cM"
     gene            1..3511
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /note="progressive ankylosis"
                     /db_xref="GeneID:11732"
                     /db_xref="MGI:MGI:3045421"
     exon            1..423
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..1806
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /note="progressive ankylosis protein; fn54 protein; ATP
                     carrier protein ANKH; progressive ankylosis protein
                     homolog"
                     /codon_start=1
                     /product="mineralization regulator ANKH"
                     /protein_id="NP_065065.3"
                     /db_xref="CCDS:CCDS27402.1"
                     /db_xref="GeneID:11732"
                     /db_xref="MGI:MGI:3045421"
                     /translation="MVKFPALTHYWPLIRFLVPLGITNIAIDFGEQALNRGIAAVKED
                     AVEMLASYGLAYSLMKFFTGPMSDFKNVGLVFVNSKRDRAKAVLCMVVAGAIAAVFHT
                     LIAYSDLGYYIINKLHHVDESVGSKTRRAFLYLAAFPFMDAMAWTHAGILLKHKYSFL
                     VGCASISDVIAQVVFVAILLHSHLECREPLLIPILSLYMGALVRCTTLCLGYYRNIHD
                     IIPDRSGPELGGDATIRKMLSFWWPLALILATQRISRPIVNLFVSRDLGGSSAATEAV
                     AILTATYPVGHMPYGWLTEIRAVYPAFDKNNPSNKLANTSNTVTSAHIKKFTFVCMAL
                     SLTLCFVMFWTPNVSEKILIDIIGVDFAFAELCVIPLRIFSFFPVPVTVRAHLTGWLM
                     TLKKTFVLAPSSVLRIIVLITSLVVLPYLGVHGATLGVGSLLAGFVGESTMVALAACY
                     VYRKQKKKMENESATEGEDSAMTDMPPTEEVTDIVEMREENE"
     exon            424..640
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            641..759
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            760..843
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            844..1014
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1149
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1242
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1338
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1468
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1592
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1692
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..3498
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3023..3028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /note="hexamer: AATAAA"
     polyA_site      3043
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /note="major polyA site"
     regulatory      3475..3480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
                     /note="hexamer: ATTAAA"
     polyA_site      3498
                     /gene="Ank"
                     /gene_synonym="Ankh; D15Ertd221e; mKIAA1581"
ORIGIN      
        1 agagcgctta taatggagcc gctgtcagca gaagcctctg ctgccgccgc cgccgccgcc
       61 gccgctgccg ccgctgccgt ccctgtccct cctctctttc tccccggcag atctttgttg
      121 tgtgggaggg cagcggggat ggacttgagc tcgcggctct cctgctagag cagcagcgct
      181 tggagaggac gccgccgccg ccgcgagcag ccgccgcccc aggccccgcc ggccgcggcc
      241 tccgtccccg cgctgcgctc cgcgcgcctc ccagcacagt gccctcgcgg cggcagatga
      301 gtgtggggtc agcccacggc ggggactatg gtgaaattcc cggcgctcac gcactactgg
      361 cccctgatcc ggttcctggt gcccctcggc atcaccaaca tagccatcga cttcggggag
      421 caggccttga accggggcat cgctgcagtc aaggaggatg cagtagaaat gctggccagc
      481 tacgggctgg cgtattcttt gatgaagttc ttcacggggc ccatgagtga cttcaaaaat
      541 gtgggcctgg tgttcgtgaa cagcaagaga gacagggcca aagccgtcct ctgcatggtg
      601 gtggccggtg ccatcgctgc cgtcttccac accctgatag cctacagtga cttagggtac
      661 tacatcatca acaagctgca tcatgtggat gagtctgtgg ggagcaaaac acgaagggcc
      721 ttcctgtatc tcgctgcctt cccttttatg gatgccatgg cgtggactca tgctggcatt
      781 ctcttaaaac acaaatacag tttcctggtg ggatgtgcct caatctcaga tgtcatagct
      841 caggttgttt tcgtagccat tttgcttcac agtcacctgg aatgccgaga accgctgctc
      901 atccccatcc tgtctctgta catgggtgcg cttgtgcgct gcaccacact gtgcctgggc
      961 tactacagga acatccacga catcatcccc gacaggagcg gcccagagct ggggggtgat
     1021 gcaaccataa gaaagatgct gagcttctgg tggcccctgg ctctgatcct ggccacgcag
     1081 cgaatcagtc ggcccattgt caacctcttc gtgtcccggg accttggtgg cagctctgct
     1141 gctacagagg cagtggccat tctgacagcc acctaccccg tgggtcatat gccatatggc
     1201 tggttgacag aaatccgggc tgtctaccct gcttttgaca agaataaccc cagcaataaa
     1261 ctggcgaaca caagcaacac ggtcacctca gcccacatca agaagttcac ctttgtctgc
     1321 atggcgctgt cgctgacgct ctgttttgtc atgttctgga cccccaacgt ctctgagaag
     1381 attctgatag atatcattgg agtggacttt gcctttgcag aactctgtgt cattcctctg
     1441 cgtatcttct ccttcttccc agtgccagtg actgtgagag ctcatctcac tggatggctg
     1501 atgacactga agaaaacctt tgtgctggcg cccagctccg tgctgcgcat catcgtcctc
     1561 atcaccagcc ttgtggtcct gccgtacctg ggggtgcacg gagccacact aggtgtgggc
     1621 tcccttctag cagggtttgt gggagaatct accatggttg cccttgcagc atgctatgtc
     1681 tatcgaaaac agaaaaagaa gatggagaat gagtcagcca ccgagggaga agactcggcc
     1741 atgaccgaca tgcctccaac agaggaggtc acagacatcg tagagatgag agaagaaaat
     1801 gagtaagcac aggctgctgg gggccaccgc agggacagtc aggacaacaa ctgtcgtctc
     1861 tttcctcctc ctcctcccat caagttgttt tctgttgttt aatttttatt cttggttatg
     1921 aaagaggcct tgatttagag gtttcgtata aattctctag catactgggt atgctcaccg
     1981 atgcagggac ctgaagaaag gtctttactg tcgctttgta actcagaatc gctgacttca
     2041 cgcccctgct tcataaaacc caaaagatag agctgcctct tggtcaacgt ttctactccc
     2101 ttggacaatc tccactttgg aaccaaagga cttgggccag acttttcctg ttcatgtttg
     2161 cctcctccta agaatcaaca ggttgaagct cagcctctct tgacttgctc cccaacactg
     2221 tggctctgga gtcatgaact atctgccgca catactggtg ggccccaggc tgcagcccac
     2281 agtctccctg ttcccagagg aagggctggt ggccctgctg ggccaacgta gtgggaattt
     2341 aatctcctgt agaaattggg tcagtcacca actgacttga tcgtcagcat cccattgttt
     2401 tcctggtttc actgagttgc cgcaccccac agtgtatata catgagctga cttttcagag
     2461 ctgtcccgca agtgcagctc cagtgtcagc accctccgca tgagtttccc tgaaggcttg
     2521 cgtttactcg ccttccctga agatggcact agagcgagaa gtggagcgtt ctaactgtcc
     2581 atttctctaa ctgtccattt cgttttcaca gtgagcaaag ctttaagtcg taatctagca
     2641 tcctaatgcc agggtcttgt ctcgtaactt ctgatgtaga tgtgccgcct atttctgcag
     2701 gacaggtaac cgagtgtact atggtactcc cctccacacc ataaagcaag acattttata
     2761 aacagtatca aggtcactat gtgatacccc ggaaataatg cattggaaat ccacttagtg
     2821 cagtatattt ttctaagttt tggaaagcgg gttttttcct ttaaaaaaaa ttatggacac
     2881 agttcactaa attcctgatt tagtcaaagt aactagactg aaagaaccta aacaaaacaa
     2941 atattttaaa gatataaata tatgctgtat atgttatgta atttatttta ggctataata
     3001 catttcctat tttcgcattt tcaataaaat gtctctaata caattcagtg attgccttca
     3061 tgctccacat acctacagct tactcgggtt gtaccagtga ccgctgtatg ttacagcctt
     3121 ctacacgtcc cttgtgtatg aatgtaaggg cctacagatc caattgatgg gcaacctcgg
     3181 gaggaaatgg atccactact gtagacaggc aaaggtggga agggcggagg gaaaagggcg
     3241 gaaggcgggc ctcttgtgct tattattttg gtgttgtgta acctcatgtt aagactgtca
     3301 gggctacaca ggctcgcatt tccatttcta gatacccagt gtccctcata tgtgcccacc
     3361 atgggaacaa atcctatgtg cttccgtagc tgcttgtggg tgacgtcagg ggcagatctg
     3421 aaagataact gaaagtctta attgtattgg ctttttaaag tacatgacta gaaaattaaa
     3481 cagcaatatt ttttaaaaaa aaaaaaaaaa a
//
