LOCUS       NM_007426               3560 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus angiopoietin 2 (Angpt2), mRNA.
ACCESSION   NM_007426
VERSION     NM_007426.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Pastura,P., McDaniel,C.G., Alharbi,S., Fox,D., Coleman,B.,
            Malik,P., Adams,D.M. and Le Cras,T.D.
  TITLE     NRASQ61R mutation drives elevated angiopoietin-2 expression in
            human endothelial cells and a genetic mouse model
  JOURNAL   Pediatr Blood Cancer 71 (7), e31032 (2024)
   PUBMED   38711167
  REMARK    GeneRIF: NRAS[Q61R] mutation drives elevated angiopoietin-2
            expression in human endothelial cells and a genetic mouse model.
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Meder,L., Orschel,C.I., Otto,C.J., Koker,M., Bragelmann,J.,
            Ercanoglu,M.S., Dahling,S., Compes,A., Selenz,C., Nill,M.,
            Dietlein,F., Florin,A., Eich,M.L., Borchmann,S., Odenthal,M.,
            Blazquez,R., Hilberg,F., Klein,F., Hallek,M., Buttner,R.,
            Reinhardt,H.C. and Ullrich,R.T.
  TITLE     Blocking the angiopoietin-2-dependent integrin beta-1 signaling
            axis abrogates small cell lung cancer invasion and metastasis
  JOURNAL   JCI Insight 9 (10), e166402 (2024)
   PUBMED   38775153
  REMARK    GeneRIF: Blocking the angiopoietin-2-dependent integrin beta-1
            signaling axis abrogates small cell lung cancer invasion and
            metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3560)
  AUTHORS   Wang,X., Huang,J., Liu,J., Sun,Y., Feng,X., Jin,Y. and Zhou,W.
  TITLE     Silencing ANGPT2 alleviates ulcerative colitis by regulating
            autophagy-mediated NLRP3 inflammasome inactivation via the mTOR
            signaling pathway
  JOURNAL   Braz J Med Biol Res 57, e13379 (2024)
   PUBMED   38808888
  REMARK    GeneRIF: Silencing ANGPT2 alleviates ulcerative colitis by
            regulating autophagy-mediated NLRP3 inflammasome inactivation via
            the mTOR signaling pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3560)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3560)
  AUTHORS   Ao,X., Li,Y., Jiang,T., Li,C., Lian,Z., Wang,L., Zhang,Z. and
            Huang,M.
  TITLE     Angiopoietin-2 Promotes Mechanical Stress-induced Extracellular
            Matrix Degradation in Annulus Fibrosus Via the HIF-1alpha/NF-kappaB
            Signaling Pathway
  JOURNAL   Orthop Surg 15 (9), 2410-2422 (2023)
   PUBMED   37475697
  REMARK    GeneRIF: Angiopoietin-2 Promotes Mechanical Stress-induced
            Extracellular Matrix Degradation in Annulus Fibrosus Via the
            HIF-1alpha/NF-kappaB Signaling Pathway.
REFERENCE   6  (bases 1 to 3560)
  AUTHORS   Gale,N.W. and Yancopoulos,G.D.
  TITLE     Growth factors acting via endothelial cell-specific receptor
            tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular
            development
  JOURNAL   Genes Dev 13 (9), 1055-1066 (1999)
   PUBMED   10323857
  REMARK    Review article
REFERENCE   7  (bases 1 to 3560)
  AUTHORS   Valenzuela,D.M., Griffiths,J.A., Rojas,J., Aldrich,T.H.,
            Jones,P.F., Zhou,H., McClain,J., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A., Huang,T., Papadopoulos,N., Maisonpierre,P.C.,
            Davis,S. and Yancopoulos,G.D.
  TITLE     Angiopoietins 3 and 4: diverging gene counterparts in mice and
            humans
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 1904-1909 (1999)
   PUBMED   10051567
REFERENCE   8  (bases 1 to 3560)
  AUTHORS   Takakura,N., Huang,X.L., Naruse,T., Hamaguchi,I., Dumont,D.J.,
            Yancopoulos,G.D. and Suda,T.
  TITLE     Critical role of the TIE2 endothelial cell receptor in the
            development of definitive hematopoiesis
  JOURNAL   Immunity 9 (5), 677-686 (1998)
   PUBMED   9846489
REFERENCE   9  (bases 1 to 3560)
  AUTHORS   Maisonpierre,P.C., Suri,C., Jones,P.F., Bartunkova,S.,
            Wiegand,S.J., Radziejewski,C., Compton,D., McClain,J.,
            Aldrich,T.H., Papadopoulos,N., Daly,T.J., Davis,S., Sato,T.N. and
            Yancopoulos,G.D.
  TITLE     Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo
            angiogenesis
  JOURNAL   Science 277 (5322), 55-60 (1997)
   PUBMED   9204896
REFERENCE   10 (bases 1 to 3560)
  AUTHORS   Fujisawa-Sehara,A., Hanaoka,K., Hayasaka,M., Hiromasa-Yagami,T. and
            Nabeshima,Y.
  TITLE     Upstream region of the myogenin gene confers transcriptional
            activation in muscle cell lineages during mouse embryogenesis
  JOURNAL   Biochem Biophys Res Commun 191 (2), 351-356 (1993)
   PUBMED   8384837
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN539462.1, CJ182273.1,
            BC138309.1 and AC122206.4.
            
            On Jan 25, 2013 this sequence version replaced NM_007426.3.
            
            Summary: This gene encodes an endothelial cell (EC)-derived
            regulator of angiogenesis and ligand for endothelial-specific
            receptor tyrosine kinase. The encoded protein acts as an
            anti-apoptotic factor for stressed ECs and a proapoptotic factor
            for resting ECs. [provided by RefSeq, Jan 2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143974.1, AK019860.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                CN539462.1         1-11
            12-28               CJ182273.1         12-28
            29-106              CN539462.1         30-107
            107-1860            BC138309.1         1-1754
            1861-3560           AC122206.4         35246-36945
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 10.3 cM"
     gene            1..3560
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /note="angiopoietin 2"
                     /db_xref="GeneID:11601"
                     /db_xref="MGI:MGI:1202890"
     exon            1..572
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     CDS             285..1775
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /codon_start=1
                     /product="angiopoietin-2 precursor"
                     /protein_id="NP_031452.2"
                     /db_xref="CCDS:CCDS22125.1"
                     /db_xref="GeneID:11601"
                     /db_xref="MGI:MGI:1202890"
                     /translation="MWQIIFLTFGWDLVLASAYSNFRKSVDSTGRRQYQVQNGPCSYT
                     FLLPETDSCRSSSSPYMSNAVQRDAPLDYDDSVQRLQVLENILENNTQWLMKLENYIQ
                     DNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTRKLTDVEAQVLNQTTRLELQL
                     LQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMKEQKDELQV
                     LVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKE
                     EQTTFRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVD
                     FQRTWKEYKEGFGSPLGEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYL
                     AGEESNYRIHLTGLTGTAGKISSISQPGSDFSTKDSDNDKCICKCSQMLSGGWWFDAC
                     GPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF"
     exon            573..728
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            729..850
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1083
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1214
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1316
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1483
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1614
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..3560
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2470..2475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /note="hexamer: ATTAAA"
     polyA_site      2492
                     /gene="Angpt2"
                     /gene_synonym="Agpt2; Ang-2; Ang2"
                     /note="major polyA site"
ORIGIN      
        1 tgattgattc ggatactgac actgtagact caggggagaa acaaagagtc cgtgcagacc
       61 tctggagtga gcagggctgc tccttcctct caggacagct ccgagtgtgc cggggagaag
      121 agaagagaag agacaggcac tgggaaagag cctgctgcgg gacggagaag gctctcactg
      181 atggacttat tcacacggca cagccctgtg ccttagacag cagctgagag ctcaggacgc
      241 aagtttgctg aactcacagt ttagaaccca aaaagagaga gagaatgtgg cagatcattt
      301 tcctaacttt tggctgggat cttgtcttgg cctcagccta cagtaacttt aggaagagcg
      361 tggacagcac aggcagaagg cagtaccagg tccagaacgg accctgcagc tacacgttcc
      421 tgctgccgga gaccgacagc tgccgatctt cctccagccc ctacatgtcc aatgccgtgc
      481 agagggatgc acccctcgac tacgacgact cagtgcaaag gctgcaggtg ctggagaaca
      541 ttctagagaa caacacacag tggctgatga agctggagaa ttacattcag gacaacatga
      601 agaaggagat ggtggagatc caacagaatg tggtgcagaa ccagacagct gtgatgatag
      661 agattggaac cagcttgctg aaccagacag cagcacaaac tcggaaactg actgatgtgg
      721 aagcccaagt actaaaccag acgacaagac tcgagctgca gcttctccaa cattctattt
      781 ctaccaacaa attggaaaag cagattttgg atcagaccag tgaaataaac aagctacaaa
      841 ataagaacag cttcctagaa cagaaagttc tggacatgga gggcaagcac agcgagcagc
      901 tacagtccat gaaggagcag aaggacgagc tccaggtgct ggtgtccaag cagagctctg
      961 tcattgacga gctggagaag aagctggtga cagccacggt caacaactcg ctccttcaga
     1021 agcagcagca tgacctaatg gagaccgtca acagcttgct gaccatgatg tcatcaccca
     1081 actccaagag ctcggttgct atccgtaaag aagagcaaac caccttcaga gactgtgcgg
     1141 aaatcttcaa gtcaggactc accaccagtg gcatctacac actgaccttc cccaactcca
     1201 cagaggagat caaggcctac tgtgacatgg acgtgggtgg aggagggtgg acagtcatcc
     1261 aacaccgaga agatggcagt gtggacttcc agaggacgtg gaaagaatac aaagagggct
     1321 tcgggagccc tctgggagag tactggctgg gcaatgagtt tgtctcccag ctgaccggtc
     1381 agcaccgcta cgtgcttaag atccagctga aggactggga aggcaacgag gcgcattcgc
     1441 tgtatgatca cttctacctc gctggtgaag agtccaacta caggattcac cttacaggac
     1501 tcacggggac cgcgggcaaa ataagtagca tcagccaacc aggaagtgat tttagcacaa
     1561 aggattcgga caatgacaaa tgcatctgca agtgttccca gatgctctca ggaggctggt
     1621 ggtttgacgc atgtggtcct tccaacttga atggacagta ctacccacaa aaacagaata
     1681 caaataagtt taacggtatc aagtggtact actggaaggg gtccggctac tcgctcaagg
     1741 ccacaaccat gatgatccgg ccagcagatt tctaaatgcc tgcctacact accagaagaa
     1801 cttgctgcat ccaaagatta actccaaggc actgagagac accaatgcat agcagcccct
     1861 ttccacatca ggaagtgctc ctgggggtgg ggagggtctg tgtgtaccag actgaagcgc
     1921 atcacttaag cctgcaccgc taaccaacca aaggcactgc agtctggaga aacacttctg
     1981 ggaaggttgt ggctgaggat cagaaggaca gcgtgcagac tctgtcacag ggaagaatgt
     2041 tccgtgggag ttcagcagta aataactgga aaacagaaca cttagatggt gcagataaat
     2101 cttgggacca cattcctcta agcacggttt ctagagtgaa tacattcaca gctcggctgt
     2161 cacaatgaca aggccgtgtc ctcgcactgt ggcagccagt atccagggac ttctaagtgg
     2221 tgggcacagg ttatcatctg gagaagcaca cattcattgt tttcctcttg ggtgctttac
     2281 atgttcattt gaaaacaaca catttaccta tcttgatggc ttagttttta atggctggct
     2341 actatttact atatggcaaa aatgcccaca tctctggaat aaccaccaaa taagcgccat
     2401 gttggtgaat gcggagactg tactattttg ttttcttcct ggctgttaaa tatgaaggta
     2461 tttttagtaa ttaaatataa gttattagtt gaggtgatta tgtttaattt ttacgataat
     2521 atttacgatt ttgttctttg gtctcacata cacacacaca gacacacaca cacacagaca
     2581 cacacacaca gacacacaca ctcccgtgtc ttgtaacaag gaagactgct gttaatgacg
     2641 aaaatgctgt ttcaagaaga agaacttcgc cactgagcaa agtgctctag cctagagatg
     2701 tgctccctaa tttaacaaga taatatttta gaaaatgaag ctaaaactta atttatagtg
     2761 gaggaaaatc accctacctg ggacatctac aaagccataa ttctctgaat taacttttag
     2821 aaaaaattag tttgcctcag taacattttt aatatccact aacaagctga aagtgtttca
     2881 gactgtaaag ccaaaatgtc ctgctcacaa tgtaaccaga tacccctaga aaaagtttca
     2941 gaacacaact ttatgcttag aattgtcaaa ccttaactcc ttctgttcta accctcctaa
     3001 gcctgcagag tggcctctgc agcaatgaac acttgtacag agcactagtg caatttctcc
     3061 tgtagccttc cttgtctgtc tgtccatcca tccatccatc tgtccatcct tcctttctag
     3121 gtgctatttt ttaagtttat ttctataatg caaaggttct caaccttcgt atgctgagac
     3181 actgtaacac agttcctcat gttgtagtga cacccagcca taaagttatt tcactcccgt
     3241 aagtttgcta ctgttatgaa tagtgatgta agtatctgat tgcaggatag ctgatatgtg
     3301 accccaaaag gtctctgccc acaggctgag aaccaacgcc ttaacccatc tcctttcaca
     3361 cagccttggt gtgttctctg gtcaatccac agactcgggg tagccaaggc gctcttctcc
     3421 tctgccacgt tcacttcctg ccagctaact ccagagtgaa gaactctgtc cctgcctttt
     3481 gaccactgtt tctattatta catttaggaa atgtcgacag ggccaacatg gctttgtacc
     3541 cagttaaaat atgtgacacc
//
