LOCUS       NM_008569               8967 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus anaphase promoting complex subunit 1 (Anapc1), mRNA.
ACCESSION   NM_008569
VERSION     NM_008569.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Ferguson,C.J., Urso,O., Bodrug,T., Gassaway,B.M., Watson,E.R.,
            Prabu,J.R., Lara-Gonzalez,P., Martinez-Chacin,R.C., Wu,D.Y.,
            Brigatti,K.W., Puffenberger,E.G., Taylor,C.M., Haas-Givler,B.,
            Jinks,R.N., Strauss,K.A., Desai,A., Gabel,H.W., Gygi,S.P.,
            Schulman,B.A., Brown,N.G. and Bonni,A.
  TITLE     APC7 mediates ubiquitin signaling in constitutive heterochromatin
            in the developing mammalian brain
  JOURNAL   Mol Cell 82 (1), 90-105 (2022)
   PUBMED   34942119
REFERENCE   3  (bases 1 to 8967)
  AUTHORS   Steinmetz,C.C., Tatavarty,V., Sugino,K., Shima,Y., Joseph,A.,
            Lin,H., Rutlin,M., Lambo,M., Hempel,C.M., Okaty,B.W., Paradis,S.,
            Nelson,S.B. and Turrigiano,G.G.
  TITLE     Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting
            AMPAR into the Recycling Endosomal Pathway
  JOURNAL   Cell Rep 16 (10), 2711-2722 (2016)
   PUBMED   27568566
REFERENCE   4  (bases 1 to 8967)
  AUTHORS   Cao,J., Dai,X., Wan,L., Wang,H., Zhang,J., Goff,P.S.,
            Sviderskaya,E.V., Xuan,Z., Xu,Z., Xu,X., Hinds,P., Flaherty,K.T.,
            Faller,D.V., Goding,C.R., Wang,Y., Wei,W. and Cui,R.
  TITLE     The E3 ligase APC/C(Cdh1) promotes ubiquitylation-mediated
            proteolysis of PAX3 to suppress melanocyte proliferation and
            melanoma growth
  JOURNAL   Sci Signal 8 (392), ra87 (2015)
   PUBMED   26329581
  REMARK    GeneRIF: findings suggest a tumor suppressor role for APC/C(Cdh1)
            in melanocytes and that targeting PAX3 may be a strategy for
            treating melanoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8967)
  AUTHORS   Sun,J., Karoulia,Z., Wong,E.Y., Ahmed,M., Itoh,K. and Xu,P.X.
  TITLE     The phosphatase-transcription activator EYA1 is targeted by
            anaphase-promoting complex/Cdh1 for degradation at M-to-G1
            transition
  JOURNAL   Mol Cell Biol 33 (5), 927-936 (2013)
   PUBMED   23263983
  REMARK    GeneRIF: EYA1 is efficiently degraded during mitotic exit in a
            ANAPC1-dependent manner and these two proteins physically interact.
REFERENCE   6  (bases 1 to 8967)
  AUTHORS   Fan,M., Rhee,J., St-Pierre,J., Handschin,C., Puigserver,P., Lin,J.,
            Jaeger,S., Erdjument-Bromage,H., Tempst,P. and Spiegelman,B.M.
  TITLE     Suppression of mitochondrial respiration through recruitment of
            p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK
  JOURNAL   Genes Dev 18 (3), 278-289 (2004)
   PUBMED   14744933
REFERENCE   7  (bases 1 to 8967)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 8967)
  AUTHORS   Davis,E.S., Wille,L., Chestnut,B.A., Sadler,P.L., Shakes,D.C. and
            Golden,A.
  TITLE     Multiple subunits of the Caenorhabditis elegans anaphase-promoting
            complex are required for chromosome segregation during meiosis I
  JOURNAL   Genetics 160 (2), 805-813 (2002)
   PUBMED   11861581
REFERENCE   9  (bases 1 to 8967)
  AUTHORS   Jorgensen,P.M., Graslund,S., Betz,R., Stahl,S., Larsson,C. and
            Hoog,C.
  TITLE     Characterisation of the human APC1, the largest subunit of the
            anaphase-promoting complex
  JOURNAL   Gene 262 (1-2), 51-59 (2001)
   PUBMED   11179667
REFERENCE   10 (bases 1 to 8967)
  AUTHORS   Starborg,M., Brundell,E., Gell,K. and Hoog,C.
  TITLE     A novel murine gene encoding a 216-kDa protein is related to a
            mitotic checkpoint regulator previously identified in Aspergillus
            nidulans
  JOURNAL   J Biol Chem 269 (39), 24133-24137 (1994)
   PUBMED   7929068
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077847.1, BC139002.1, AK034858.1, BE987509.1 and BB676785.1.
            
            On Apr 29, 2009 this sequence version replaced NM_008569.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090134.1, X80169.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2990              AK077847.1         2-2991
            2991-6175           BC139002.1         2972-6156
            6176-8343           AK034858.1         1707-3874
            8344-8682           BE987509.1         66-404
            8683-8967           BB676785.1         159-443
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.63 cM"
     gene            1..8967
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="anaphase promoting complex subunit 1"
                     /db_xref="GeneID:17222"
                     /db_xref="MGI:MGI:103097"
     exon            1..243
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            244..480
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..6102
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="cyclosome subunit 1; mitotic checkpoint regulator;
                     testis-specific gene 24 protein; meiotic check point
                     regulator"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 1"
                     /protein_id="NP_032595.2"
                     /db_xref="CCDS:CCDS16715.1"
                     /db_xref="GeneID:17222"
                     /db_xref="MGI:MGI:103097"
                     /translation="MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQP
                     ASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIW
                     SKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMN
                     MHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLD
                     EITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKP
                     EEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSS
                     PSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFN
                     DSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQL
                     QLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGK
                     VFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKL
                     NDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQA
                     IKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFD
                     FEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKD
                     EDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLK
                     LDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEG
                     MPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENR
                     FTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSE
                     AVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSE
                     DLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFT
                     LFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLK
                     IAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMT
                     SIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQ
                     GTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLN
                     VPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKT
                     NNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRE
                     NSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFSCLHKFAKD
                     FMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNY
                     GFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLY
                     VLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKV
                     KGPRYWELLIDLSKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANR
                     NSEARAFKPETISSFTSDPALLSFAEYFCKPTVSMGPKQEILDLFSSILYECVAQETP
                     EMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEFFSSRSHQDRQHTYPKRGLFINS
                     EFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLVYHSVPAPRHL
                     PPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM"
     exon            481..642
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            643..694
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            695..795
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            796..878
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            879..952
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1098
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1319
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1529
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1625
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1716
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1782
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1917
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2058
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2119
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2307
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2382
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2651
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2743
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2892
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..2971
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3008
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3079
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3258
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3473
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3592
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3593..3864
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4099
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4100..4191
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4192..4326
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4327..4409
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4410..4488
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4489..4563
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4564..4640
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4641..4731
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4732..4785
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4786..4979
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            4980..5066
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5067..5184
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5185..5262
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5263..5330
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5331..5469
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5470..5556
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5557..5652
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5653..5851
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5852..5986
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     exon            5987..8967
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6319..6324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="hexamer: AATAAA"
     polyA_site      6346
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="major polyA site"
     regulatory      8325..8330
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="hexamer: AATAAA"
     polyA_site      8350
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
     regulatory      8924..8929
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
                     /note="hexamer: AATAAA"
     polyA_site      8946
                     /gene="Anapc1"
                     /gene_synonym="2610021O03Rik; Apc1; Mcpr; tsg24"
ORIGIN      
        1 gccctggcgc gaggcaggtc gggaaatcca agatggctcc agccatttga gttgttcgca
       61 agtctttggt gtcccgctgc cgcccattct tcctccggca tccgggaggg aaaagggatg
      121 agaaactggg gcggtgagga gtcatcgtga cggtcctgtc gctgctctcg ggccttcgcg
      181 gcccttgtgc ctccggggag cagccggggc tcgcaagcgc ctgacgcgtc ccgagttatt
      241 cagaaataat gttcatattt ggaacccatg tcgaacttct ctgaagaaag ggcaacgatg
      301 attgcagccg gggatttgca ggaatttgtt ccttttggcc gagaccactg caagcaccac
      361 cctaacgctc tgaaccttca gcttcgtcag ctgcagccag cctctgagtt atggtcttct
      421 gatggtgcag ctggcttggt gggatctctt caggaggtta caatccatga gaaacagaag
      481 gaaagctggc agttaagaaa aggtgtgagt gagattggag atgcagctga ctacgatgaa
      541 gagctctatg tggctgggaa catggttatc tggagcaaag ggagtaaaag ccaggcactg
      601 gcagtgtaca aagcgttcac agtggacagc actgtccagc aggcattgtg gtgtgacttc
      661 attatatcac aggataagtc tgaaaagatc tataagagcc atgaactaga gaaatgcata
      721 tgtatattac agagttcatg tatgaacatg catagcatag acgggaagga ttatatagct
      781 tccttacctt ttcaggtggc aaatgtttgg gccactaaat atggcttgct gtttgaacga
      841 tgcagttctt cacatgaggt acctccaagt ctacctagag aacctttgcc tactatgttc
      901 agcatgctgc acccactgga tgagataact ccacttgttt gtaaatccgg aagtcttttt
      961 ggctcatcac gggtacagta tgttgtagat ccagcagtga aaattgtgtt tctgaacatt
     1021 gacccctcca ttgtcatgac ctatgatgct gttcagaatg tgcattctgt gtggactctg
     1081 cggagggtaa agccagagga agagaatgct gttttaaagt tccctgagca ggcagggacc
     1141 ctgcagaatg caactactag cagctccctg acagcacatc tcagaagcct ctccaaaggc
     1201 gaatcccctg tggcttcgcc gttccagaat tactcttcca ttcacagcca gagtcgctca
     1261 acctcttcgc ccagtctaca ctctcggtcg ccttccattt ccaacatggc ggctctaagt
     1321 cgtgctcact ctcctgcctt aggtgtgcac tctttctcag gggcgcaaag attcaacctt
     1381 tcaagccaca gtcagtcacc aaagagacac agcatttctc actctccgag tggcagtttc
     1441 aacgactcat ttttggcacc agaaacagag ccaattgttc ctgagctgtg cattgatcac
     1501 ttatggactg agacacttcc gaatataaga gagaaaaatt ctcaagcctc caaggtattt
     1561 ataacgactg acctgtgtgg acagaagttc ttgtgctttt tagtggaggc ccagcttcag
     1621 ttacgctgtg taaagtttca agagagtaat gacaagactc agcttatctt tggctctgtc
     1681 accaacatac atgcaaaaga tgcagcccca gtggagaaga tacacaccat gctggtccta
     1741 gaaggcaacg ggaacctggt gctgtacaca ggagtggtgc gggtggggaa agtctttatc
     1801 cctggacttc cggctccttc cctgacaatg tccaacatga tgcctcggcc cagtaccccg
     1861 ctcgacggtg ttggtactcc aaagcctctg agtaaattgc ttggatcgat ggatgaggtg
     1921 gttctgttgt ctccagttcc agaattgagg gattcttcaa aacttaatga ttctctctac
     1981 aatgaggatt gcacattcca gcagcttgga acttacattc attctgtgcg agaccctgtt
     2041 cataacagag tcactctaga actgagcaat ggctccatgg ttcggatcac tatccctgaa
     2101 gtggccacct cggaattagt acaaacgtgt ctgcaagcaa ttaagttcat cctgccaaaa
     2161 gaagtagcca ttcaggtgct cgtcaagtgg tacaatgtgc acagtgctcc aggaggaccc
     2221 agctgtcact cagagtggag tttatttgtg atttgtctct tgaacatgat gggttataac
     2281 acagaccgct tagcatggac ccgaagtttt gactttgaag gatcactttc cccagtcatt
     2341 gcacccaaaa aagcaaggcc ttctgatact ggatctgacg aggactggga gtacttactg
     2401 aactcagagt accatcgcaa tgttgagtct catcttttga acaaatctct atgtttgact
     2461 gctttggaag tttcaaatgc aaaggatgaa gatttttcgc agaacctcag tctggattct
     2521 tctacccttc tctttgctca cattcctgca attttctttg ttcttcacct ggtttatgaa
     2581 gagctcaagt tgaatactct aatgggagaa ggaatttgtt ctcttatcga cctccttgtt
     2641 cagttggcaa gggacttaaa attggactct tacttggacc attactatcg agattcccca
     2701 actcttgtca aaaccactgg acaagtatgc accattgacc aaggtcagat gggatttatg
     2761 catcatcccc cattttttac ttctgagcca ccaagtattt atcagtgggt gagttcatgc
     2821 ctgaagggtg aagggatgcc accctatcct taccttcctg ggatttgcga gaggagcagg
     2881 ctagtggtct tgagcattgc cctctatacc cttggtgatg agagctgtgt gtctgatgaa
     2941 acttgccagt acttatccaa agtaacctca actccccaaa agccacaagc agagcaagaa
     3001 gaaaacaggt ttactttccg gcattctgct tccgtttctg ttctggccga gagattagtt
     3061 gtctggatgg ccagtgtagg attcacttta agagatctgg agactcttcc ctttgggatt
     3121 gctcttccca tcagagatgc gatttaccac tgtcgggagc agcctgattc agattggtca
     3181 gaagcggtct gtctcttgat tggacgtcag gatctttcca agcaggcttg tgaaggaaat
     3241 ttacccagag gcaaatctgt gctctcatca gaggtgtctt caggaactga ggcagaggag
     3301 gaagatgatg gcatgaatga cttgaaccat gaggttatgt cattaatatg gagtgaagat
     3361 ttacgggtgc aggatgtgcg aaggctgctt caaagtgcac agcctgtccg tgtcaatgtg
     3421 gtgcagtacc cagaactcag tgaccatgag ttcattgaag aaaaggaaaa cagactgctc
     3481 cagttgtgtc agcgaactat ggcccttcca gtaggacgag gaatgtttac cttgttctca
     3541 tatcatcctg ttccaacaga gccgttgcct gttcctaaat tgaatctgac agggcgagcc
     3601 cctccacgaa acacaactgt agatcttaat agcggaaaca tcgacgtgcc tcccaacatg
     3661 gccagctggg ccagctttca taatggtgtg gctgccggcc tgaagatagc cccagcctcc
     3721 cagatagact cagcttggat cgtttacaac aagccaaagc atgctgagtt agccaatgag
     3781 tatgccggct ttctcatggc cctgggtctg aatgggcacc ttaccaagct ggctactctc
     3841 aatatccatg actatttgac caagggccat gaaatgacaa gcattggatt gctacttggt
     3901 gtttctgctg caaagcttgg caccatggac atgtcaatta cccggctcct cagcattcac
     3961 gttcctgctc tcttaccccc gacatccaca gagcttgacg tgcctcacaa tgtccaagtg
     4021 gcggctgtgg ttggcattgg ccttgtgtat cagggcacag ctcacagaca cactgcggaa
     4081 gtcctgttgg ctgaaatagg gcggccccct ggtccagaaa tggaatactg cactgacaga
     4141 gagtcgtact ccttagctgc tggcctggcc ctgggcatgg tttgcttggg gcatggcagc
     4201 aatttgattg gcatgtctga tctcaatgtg cctgagcagc tgtatcagta catggttgga
     4261 ggccataggc gatttcaaac tggaatgcac agggagaaac ataagtctcc aagttatcag
     4321 atcaaagaag gagacaccat aaacgtggat gtgacttgtc ccggtgctac tctggcgttg
     4381 gctatgatct acttaaaaac caataacagg tccattgctg attggctgcg tgctcctgat
     4441 accatgtatt tgctagactt tgtgaaacca gaattccttt tgcttaggac acttgctcgg
     4501 tgcttgattt tgtgggatga tatcttacca aattccaagt gggttgacag caatgttcct
     4561 cagattataa gagaaaatag tatctctctg agtgaaattg aattgccttg ttcagaggac
     4621 ttgaatttgg aaaccttgtc gcaagcacac gtctacatca ttgcaggagc ctgcttgtct
     4681 ctagggtttc gatttgctgg ctcagaaaac ttatcagcat ttagctgtct gcataaattt
     4741 gcaaaagatt ttatgaatta tttatctgca cccaatgctt ctgtaacagg gccctataac
     4801 ctcgaaacct gcctgagtgt ggtcctgctg tctcttgcca tggtgatggc tggctctggg
     4861 aacctgaagg tgttgcagct ctgtcgcttt ctgcacatga agactggtgg agagatgaac
     4921 tatggcttcc acttggccca ccacatggcc ctgggccttc tctttttggg aggaggaagg
     4981 tactctttga gcacatccaa ctcatccatt gctgcccttc tctgcgccct ttacccacat
     5041 ttcccagccc acagcactga caaccggtat catctccagg cccttcggca cctctatgtg
     5101 ctagctgcag aaccaaggct cctggtacct gtggatgtgg atacaaacac accctgctat
     5161 gcccttatag aagttactta caagggcact cagtggtatg aacagaccaa agaagaactg
     5221 atggctccaa cccttcttcc agaacttcat cttttaaagc agatgaaagt taaagggcca
     5281 agatactggg aactgctcat agatttaagc aagggagaac agcacttgag gtctattctt
     5341 tcgaaggatg gagttttata tgtaaagctc agggcaggac agctctccta caaagaagac
     5401 ccaatggggt ggcagagttt gttggcacaa actgttgcta ataggaactc tgaagcccgg
     5461 gctttcaagc ctgaaacaat ttcatcattc acttctgatc cagcacttct gtcatttgct
     5521 gaatatttct gcaagccgac tgtgagcatg ggtccaaaac aggagattct ggacctcttt
     5581 tcttcgatac tttatgagtg tgttgctcag gaaaccccag agatgttgcc tgcctacata
     5641 gcaatggatc aggctctaag aagtcttaaa aagagagaca tgtcagacac atctgatctt
     5701 tggcagataa agttgatatt agaatttttc agttcccgaa gccaccagga taggcagcat
     5761 acctatccta agcgagggct cttcataaat tctgagttcc tacctgtggt gaagtgcact
     5821 gttgatgcta ccctggacca gtggctacaa gctggcggtg atgtgtgtgt gcatgcctac
     5881 ctcagtgggc agcctgttga gaagtctcag ctaaacatgc tggcctgctt ccttgtctac
     5941 cactctgtgc cagcaccacg acacttgccc cccatgggac tggaagggag cacaagcttt
     6001 gctgaactcc tctacaggtt ccgacacctg aagatgcccg tccgggcttt gctaagactg
     6061 gctcctgtgc tgcttgggaa cccacagccg atggtcatgt gaccacctct gccgagggaa
     6121 acccacccta gaaatgactg cggcacagaa tcctgatggc agcaaagtga aggcagcatt
     6181 tctgttttgt aaccaatgga actcagttgc atacgcctca ctgtgcttaa aattcatgtt
     6241 gaaaataaga cagataacgc tggtgctgtc cacgtgtcat atgatgtata aaacatcagt
     6301 taaaactcac attttgtaaa taaagatttt tggtttgttt tcaaaactat tgattgcttt
     6361 ggacttcaga gaatagatgg acaggctgag tttttaagcc ttataacttt agtggttcag
     6421 gttctgtact cagaagtgca aggcttaaaa agccacattg tgaaaatgga ctttgtgctc
     6481 tccgggagcg tttgtgtgtt gtctgttacg taacagttat cttctctaca atgagtctgt
     6541 gcttcccgtg gtggatggat gacagacagc gttactgcca gcagttttct actgaatgaa
     6601 gaattgctca gcatggagta ctggactgtg cccaagccct gaccttggtg cagctgtgta
     6661 ggctgcagct ctttggaggt aatcgaggtt aaagggcact ctaaggggga gtctatttgg
     6721 tagaatcgat gtcctgctca gaagaggaaa aagagatgat tttctctctg taccatgtga
     6781 gaactcatag cacaactgct cacaaactaa gaagattccc ttgtgaggaa ccagtcagac
     6841 agtcttgagc tttagcacct agaagtatta agcgataaac tcctgatgct tgtctgtggt
     6901 acttggtgtt tgcagcctgt gcaggctgtg catgacacct gctcaaaata ctgaaaatac
     6961 agaattaccg ccacagagga gtgttaagaa atacggaaat cagtctatct gaactaaagg
     7021 tctggaaagt gcttttgcat tctgagtttc aggacagctc atgatgcccc atgtaacagg
     7081 aacaacactc ctttcactcc atttcaacct gtgctcacag ctttctctca gaccagtgtg
     7141 actgtaaaca agtaggaact gcagaatgcc tactcgttaa cttcccgctt gtttctgaaa
     7201 aagccaatag aaattgctgc gttgggattt ggtaatgtcc atctgcctgc ctgcctgcct
     7261 gcagcaaggt aatttgtggc tgaccttgag ggcaaaatgt gctttccttc acttttagat
     7321 tagggattgc aattaaatgg cagttggtga tgaacattca atttgtatca atattgaacc
     7381 cactgtccaa gttatttttc actctcaatt gttaatggct gccatgggag gacttgaaag
     7441 agaggactga attcagtgga aacagcattg atgggccttt ttgtccgctg gacggacacc
     7501 aagaagagat tctagtgctc ttgagtcaac catgttagga tcttcagata aactatttac
     7561 tagtctccaa accatgctgt gggaaccagt cttaccatgc acagcagcca gcctcctggg
     7621 aaccacgtca agggaagagc ccgagaaaca cctgaagtgt tctagtatca aaagtggcac
     7681 ttgtgactac aagggaaggc tgattgccca ggacgttgct tcacctctga tacatgtgga
     7741 atggagacac aaggggcagc tgctttgagt gtaggtactg tccagcagac agacaatgga
     7801 gcggtgtgct cagattcttt ccccaaaggg tactgacgtc tgtccttctc tctcttggtc
     7861 taacgtggga gtgaaggaaa actgagctca gatgccaaac ttcatctaca ctactccatc
     7921 atagttctca acattctgga ctctacccta tctgctacac ccaaccctgc tctggtctgc
     7981 tgctgcctgc atggattctg agtccaagag aatcagaaac aactcaatgt gaactggaag
     8041 gtcttagcgt tggcagagct tctgaacatg ttgaaagcag ccaagcacct ttgcaatcac
     8101 atgggctttt gtgtgttttc ttgatgtttc tcccattgac ttagtcatca ctattggtca
     8161 cattatatag agcatcttgt ttaagctttt gtctttttag ctaaaaacac ttctttggta
     8221 aacttttaat actgcaatct ttttccttta gaatattttg gagtgtactt ttaagacttt
     8281 tttttttctt gaaacatatc agaattttaa aacaggaact tcataataaa tttagtaaga
     8341 tttataagca cctgccagat cttaattcct gttcttttat actccaatgt ttagctgagc
     8401 tgctagcttt tctctgtagc taatgatgtc tcaagtctct gccttgtgag gctgcagacc
     8461 acgtgtttta agaactcaag acatgttggg tcatggtgtc atggtcacct cataggtgga
     8521 gggtgggtac ctaaaacaag gacagaagcc agcagctagt gttacatggt aattttcttc
     8581 tgcttgaaac atccttccag gaagaagggg aggggagccc caagacacta aataagcatc
     8641 acgtgtctta ggaagctgaa gcttaagagt cacaaagtgc tttcccaagg ttgtagaaat
     8701 ggaactgtat gggaacagga cagaatggga cagattgact taccagcaga gctccccaga
     8761 gaaggcctgg ctgcctggga gaggagattc cctgactagt caagcagcct gcaagttgtg
     8821 tagagttgtt acccatgatg atgtgggctt tggtgataga gctgcttttt gagtcgttcc
     8881 tgctcctgga acaacacaaa cccctattcc tacaagtgac cccaataaac tcattatatt
     8941 gctaaagttg gactttagca atgtcat
//
