LOCUS       NM_001190403            1413 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus amylase 2b (Amy2b), transcript variant 1, mRNA.
ACCESSION   NM_001190403 XM_001481315 XM_001481336
VERSION     NM_001190403.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Quilichini,E., Fabre,M., Nord,C., Dirami,T., Le Marec,A.,
            Cereghini,S., Pasek,R.C., Gannon,M., Ahlgren,U. and Haumaitre,C.
  TITLE     Insights into the etiology and physiopathology of MODY5/HNF1B
            pancreatic phenotype with a mouse model of the human disease
  JOURNAL   J Pathol 254 (1), 31-45 (2021)
   PUBMED   33527355
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Wu,C.Y., DiJulio,D.H., Jacobson,K.L., McKnight,G.S. and Watson,E.L.
  TITLE     The contribution of AKAP5 in amylase secretion from mouse parotid
            acini
  JOURNAL   Am J Physiol Cell Physiol 298 (5), C1151-C1158 (2010)
   PUBMED   20164376
  REMARK    GeneRIF: These data suggest that isoproterenol-stimulated amylase
            secretion occurs via both an AKAP5/AC6/PKA complex and a
            PKA-independent, Epac pathway in mouse parotid acini.
REFERENCE   3  (bases 1 to 1413)
  AUTHORS   Sugino,H.
  TITLE     Comparative genomic analysis of the mouse and rat amylase multigene
            family
  JOURNAL   FEBS Lett 581 (3), 355-360 (2007)
   PUBMED   17223109
REFERENCE   4  (bases 1 to 1413)
  AUTHORS   Bi,Y. and Williams,J.A.
  TITLE     A role for Rho and Rac in secretagogue-induced amylase release by
            pancreatic acini
  JOURNAL   Am J Physiol Cell Physiol 289 (1), C22-C32 (2005)
   PUBMED   15743890
  REMARK    GeneRIF: Rho and Rac are both involved in CCK-induced amylase
            release in pancreatic acinar cell possibly through an effect on the
            actin cytoskeleton.
REFERENCE   5  (bases 1 to 1413)
  AUTHORS   Johnson,T.M., Rosenberg,M.P. and Meisler,M.H.
  TITLE     An insulin-responsive element in the pancreatic enhancer of the
            amylase gene
  JOURNAL   J Biol Chem 268 (1), 464-468 (1993)
   PUBMED   7678001
REFERENCE   6  (bases 1 to 1413)
  AUTHORS   Keller,S.A., Rosenberg,M.P., Johnson,T.M., Howard,G. and
            Meisler,M.H.
  TITLE     Regulation of amylase gene expression in diabetic mice is mediated
            by a cis-acting upstream element close to the pancreas-specific
            enhancer
  JOURNAL   Genes Dev 4 (8), 1316-1321 (1990)
   PUBMED   1699843
REFERENCE   7  (bases 1 to 1413)
  AUTHORS   Osborn,L., Rosenberg,M.P., Keller,S.A. and Meisler,M.H.
  TITLE     Tissue-specific and insulin-dependent expression of a pancreatic
            amylase gene in transgenic mice
  JOURNAL   Mol Cell Biol 7 (1), 326-334 (1987)
   PUBMED   2436036
REFERENCE   8  (bases 1 to 1413)
  AUTHORS   Gumucio,D.L., Wiebauer,K., Dranginis,A., Samuelson,L.C.,
            Treisman,L.O., Caldwell,R.M., Antonucci,T.K. and Meisler,M.H.
  TITLE     Evolution of the amylase multigene family. YBR/Ki mice express a
            pancreatic amylase gene which is silent in other strains
  JOURNAL   J Biol Chem 260 (25), 13483-13489 (1985)
   PUBMED   2414282
REFERENCE   9  (bases 1 to 1413)
  AUTHORS   Mikkelsen,B.M., Clark,M.E., Christiansen,G., Klintebaek,O.M.,
            Nielsen,J.T., Thomsen,K.K. and Hjorth,J.P.
  TITLE     The structure of two distinct pancreatic amylase genes in mouse
            strain YBR
  JOURNAL   Biochem Genet 23 (7-8), 511-524 (1985)
   PUBMED   2413838
REFERENCE   10 (bases 1 to 1413)
  AUTHORS   Wiebauer,K., Gumucio,D.L., Jones,J.M., Caldwell,R.M., Hartle,H.T.
            and Meisler,M.H.
  TITLE     A 78-kilobase region of mouse chromosome 3 contains salivary and
            pancreatic amylase genes and a pseudogene
  JOURNAL   Proc Natl Acad Sci U S A 82 (16), 5446-5449 (1985)
   PUBMED   2410924
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121826.3.
            
            Summary: Amylases are secreted proteins that hydrolyze
            1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides,
            and thus catalyze the first step in digestion of dietary starch and
            glycogen. The mouse genome has a cluster of several amylase genes
            that are expressed at high levels in either salivary gland or
            pancreas. This gene encodes an amylase isoenzyme produced by the
            pancreas. At least one mouse strain has a non-functional allele of
            this gene. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jan
            2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189682.89131.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849377,
                                           SAMN00849388 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               AC121826.3         116896-117080       c
            186-332             AC121826.3         116417-116563       c
            333-521             AC121826.3         114554-114742       c
            522-587             AC121826.3         113778-113843       c
            588-721             AC121826.3         112373-112506       c
            722-844             AC121826.3         111558-111680       c
            845-944             AC121826.3         111366-111465       c
            945-1063            AC121826.3         109993-110111       c
            1064-1189           AC121826.3         109758-109883       c
            1190-1413           AC121826.3         108009-108232       c
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 49.35 cM"
     gene            1..1413
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /note="amylase 2b"
                     /db_xref="GeneID:545562"
                     /db_xref="MGI:MGI:104547"
     exon            1..185
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1379
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /EC_number="3.2.1.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; amylase 2b, pancreatic; amylase 2-2,
                     pancreatic; Pancreatic alpha-amylase; 1,4-alpha-D-glucan
                     glucanohydrolase"
                     /codon_start=1
                     /product="amylase 2b isoform 1 precursor"
                     /protein_id="NP_001177332.1"
                     /db_xref="GeneID:545562"
                     /db_xref="MGI:MGI:104547"
                     /translation="MKFVLLLSLIGFCWAQYDPHTSDGRTAIVHLFEWRWVDIAKECE
                     RYLAPKGFGGVQVSPPNENIVIHNPSRPWWERYQPISYKICTRSGNEDEFRDMVTRCN
                     NVGVRIYVDAVINHMCGSGNPAGTSSTCGSYLNPNNREFPAVPYSAWDFNDNKCNGEI
                     SNYNDAYQVRNCRLSGLLDLALEKDYVRTKVIDLGGEAIKGSEYFGNGRVTEFKFGAK
                     LGTVIRKWNGEKMSYLKNWGEGWGLVPSDRALVFVDNHDNQRGHGAGGSSILTFWDAR
                     MYKMAVGFMLAHPYGFTRVMSSYRWNRNFQNGKDQNDWIGPPNNNGVTKEVTINADTT
                     CGNDWVCEHRWRQIRNMVAFRNVVNGQPFANWWDNNSNQVAFSRGNRGFIVFNNDDWA
                     LSATLQTGLPAGTYCDVISGDKVDGNCTGLKVNVGSDGKAHFSISNSAEDPFIAIHAD
                     SKL"
     exon            186..332
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            333..521
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            522..587
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            588..721
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            722..844
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            845..944
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1063
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1189
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1413
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /note="hexamer: ATTAAA"
     polyA_site      1407
                     /gene="Amy2b"
                     /gene_synonym="Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA"
                     /note="major polyA site"
ORIGIN      
        1 gacaacttca aagcaaaatg aagttcgttc tgctgctttc cctcattggg ttctgctggg
       61 ctcaatatga cccacatact tcagatggga ggactgctat tgtccacctg ttcgagtggc
      121 gctgggttga tattgccaag gaatgtgagc gatacttagc tcctaaggga tttggagggg
      181 tgcaggtctc tccacccaat gaaaatattg taattcataa cccatcaagg ccttggtggg
      241 aaagatatca accaatcagc tacaaaattt gcacaaggtc tggaaatgaa gatgaattca
      301 gagacatggt gacaaggtgc aacaatgttg gtgtccgtat ttatgtggat gctgtcatta
      361 accacatgtg tggctcaggc aatcctgcag gaacaagcag tacctgtgga agttacctca
      421 atccaaataa cagggaattc ccagcagttc catactctgc ttgggacttt aacgataata
      481 aatgtaatgg agaaattagt aactacaatg atgcttatca ggtcagaaat tgtcgtctgt
      541 ctggccttct ggatcttgca cttgagaaag attatgttcg taccaaggtt attgatctgg
      601 gtggtgaggc aattaaaggt agtgagtact ttggaaatgg ccgtgtaaca gaattcaagt
      661 ttggtgcaaa acttggcaca gttatccgca agtggaatgg agagaagatg tcctatttaa
      721 agaactgggg agaaggttgg ggtttggtgc cttctgacag agcccttgtg tttgtggaca
      781 accatgacaa tcagcgagga catggtgctg gaggatcatc catcctgaca ttctgggatg
      841 ctagaatgta caaaatggct gttggattta tgttggctca tccttatgga ttcacaagag
      901 taatgtcaag ttaccgttgg aatagaaatt tccagaatgg aaaagatcag aatgactgga
      961 ttggaccacc taataacaat ggagtaacaa aagaagtgac cattaatgca gacactactt
     1021 gtggcaatga ctgggtctgt gaacatagat ggcgtcaaat aaggaacatg gttgccttca
     1081 ggaatgtagt caatggtcag ccttttgcaa actggtggga taataacagc aaccaagtag
     1141 cttttagcag aggaaacaga ggcttcattg tctttaacaa tgatgactgg gctttgtcag
     1201 ccactttaca gactggtctt cctgctggca catactgtga tgtcatctct ggagataagg
     1261 tcgatggcaa ttgcactgga cttaaagtaa atgttggcag tgatggtaaa gctcactttt
     1321 ccattagtaa ctctgctgag gacccattta ttgcaatcca tgctgactca aaactgtaag
     1381 aatcaaatta aagacatgta gagagcacat caa
//
