LOCUS       NM_001411494            3586 bp    mRNA    linear   ROD 08-APR-2024
DEFINITION  Mus musculus amylase 2a1 (Amy2a1), mRNA.
ACCESSION   NM_001411494 XM_036163493
VERSION     NM_001411494.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Sugino,H.
  TITLE     Comparative genomic analysis of the mouse and rat amylase multigene
            family
  JOURNAL   FEBS Lett 581 (3), 355-360 (2007)
   PUBMED   17223109
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Kockel,L., Strom,A., Delacour,A., Nepote,V., Hagenbuchle,O.,
            Wellauer,P.K. and Herrera,P.L.
  TITLE     An amylase/Cre transgene marks the whole endoderm but the primordia
            of liver and ventral pancreas
  JOURNAL   Genesis 44 (6), 287-296 (2006)
   PUBMED   16786601
  REMARK    GeneRIF: Generation of a new transgenic mouse that should be useful
            to target endoderm at early stages, without affecting the liver or
            ventral pancreas before embryonic day E12.5.
REFERENCE   3  (bases 1 to 3586)
  AUTHORS   Osborn,L., Rosenberg,M.P., Keller,S.A. and Meisler,M.H.
  TITLE     Tissue-specific and insulin-dependent expression of a pancreatic
            amylase gene in transgenic mice
  JOURNAL   Mol Cell Biol 7 (1), 326-334 (1987)
   PUBMED   2436036
REFERENCE   4  (bases 1 to 3586)
  AUTHORS   Gumucio,D.L., Wiebauer,K., Dranginis,A., Samuelson,L.C.,
            Treisman,L.O., Caldwell,R.M., Antonucci,T.K. and Meisler,M.H.
  TITLE     Evolution of the amylase multigene family. YBR/Ki mice express a
            pancreatic amylase gene which is silent in other strains
  JOURNAL   J Biol Chem 260 (25), 13483-13489 (1985)
   PUBMED   2414282
REFERENCE   5  (bases 1 to 3586)
  AUTHORS   Mikkelsen,B.M., Clark,M.E., Christiansen,G., Klintebaek,O.M.,
            Nielsen,J.T., Thomsen,K.K. and Hjorth,J.P.
  TITLE     The structure of two distinct pancreatic amylase genes in mouse
            strain YBR
  JOURNAL   Biochem Genet 23 (7-8), 511-524 (1985)
   PUBMED   2413838
REFERENCE   6  (bases 1 to 3586)
  AUTHORS   Wiebauer,K., Gumucio,D.L., Jones,J.M., Caldwell,R.M., Hartle,H.T.
            and Meisler,M.H.
  TITLE     A 78-kilobase region of mouse chromosome 3 contains salivary and
            pancreatic amylase genes and a pseudogene
  JOURNAL   Proc Natl Acad Sci U S A 82 (16), 5446-5449 (1985)
   PUBMED   2410924
REFERENCE   7  (bases 1 to 3586)
  AUTHORS   Bodary,S., Grossi,G., Hagenbuchle,O. and Wellauer,P.K.
  TITLE     Members of the Amy-2 alpha-amylase gene family of mouse strain CE/J
            contain duplicated 5' termini
  JOURNAL   J Mol Biol 182 (1), 1-10 (1985)
   PUBMED   2987507
REFERENCE   8  (bases 1 to 3586)
  AUTHORS   Tosi,M., Bovey,R., Astolfi,S., Bodary,S., Meisler,M. and
            Wellauer,P.K.
  TITLE     Multiple non-allelic genes encoding pancreatic alpha-amylase of
            mouse are expressed in a strain-specific fashion
  JOURNAL   EMBO J 3 (12), 2809-2816 (1984)
   PUBMED   6098446
REFERENCE   9  (bases 1 to 3586)
  AUTHORS   Dranginis,A., Morley,M., Nesbitt,M., Rosenblum,B.B. and
            Meisler,M.H.
  TITLE     Independent regulation of nonallelic pancreatic amylase genes in
            diabetic mice
  JOURNAL   J Biol Chem 259 (19), 12216-12219 (1984)
   PUBMED   6207174
REFERENCE   10 (bases 1 to 3586)
  AUTHORS   Strahler,J.R. and Meisler,M.
  TITLE     Two distinct pancreatic amylase genes are active in YBR mice
  JOURNAL   Genetics 101 (1), 91-102 (1982)
   PUBMED   6180955
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102926.14 and LXEJ02010129.1.
            
            On Aug 26, 2022 this sequence version replaced XM_036163493.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X02579.1, SRR5189676.70137.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               AC102926.14        172633-172817
            186-332             AC102926.14        173148-173294
            333-521             AC102926.14        174349-174537
            522-752             AC102926.14        175290-175520
            753-762             AC102926.14        177186-177195
            763-886             LXEJ02010129.1     140606-140729
            887-1009            LXEJ02010129.1     141407-141529
            1010-1109           LXEJ02010129.1     141622-141721
            1110-1228           LXEJ02010129.1     142889-143007
            1229-1354           LXEJ02010129.1     143116-143241
            1355-3586           LXEJ02010129.1     144819-147050
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 49.35 cM"
     gene            1..3586
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /note="amylase 2a1"
                     /db_xref="GeneID:100043207"
                     /db_xref="MGI:MGI:104548"
     exon            1..185
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1544
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /EC_number="3.2.1.1"
                     /note="amylase 2a1, pancreatic; amylase 2-1, pancreatic;
                     Pancreatic alpha-amylase; 1,4-alpha-D-glucan
                     glucanohydrolase"
                     /codon_start=1
                     /product="amylase 2a1 precursor"
                     /protein_id="NP_001398423.1"
                     /db_xref="GeneID:100043207"
                     /db_xref="MGI:MGI:104548"
                     /translation="MKFVLLLSLIGFCWAQYDPHTSDGRTAIVHLFEWRWVDIAKECE
                     RYLAPKGFGGVQVSPPNENVVVHNPSRPWWERYQPISYKICTRSGNEDEFRDMVTRCN
                     NVGVRIYVDAVINHMCGAGNPAGTSSTCGSYLNPNNREFPAVPYSAWDFNDNKCNGEI
                     DNYNDAYQVRNCRLTGLLDLALEKDYVRTKVADYMNHLIDIGVAGFRLDAAKHMWPGD
                     IKAVLDKLHNLNTKWFSQGSRPFIFQEVIDLGGEAIKGSEYFGNGRVTEFKYGAKLGT
                     VIRKWNGEKMSYLKNWGEGWGMVPSDRALVFVDNHDNQRGHGAGGSSILTFWDARMYK
                     MAVGFMLAHPYGFTRVMSSYRWNRNFQNGKDQNDWIGPPNNNGVTKEVTINADTTCGN
                     DWVCEHRWRQIRNMVAFRNVVNGQPFSNWWDNNSNQVAFSRGNRGFIVFNNDDWALSA
                     TLQTGLPAGTYCDVISGDKVDGNCTGLRVNVGSDGKAHFSISNSAEDPFIAIHADSKL
                     "
     exon            186..332
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            333..521
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            522..752
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            753..886
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1009
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1109
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1228
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1354
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..3586
                     /gene="Amy2a1"
                     /gene_synonym="Amy2; Amy2-1; Amy2a; mAmy2-6; PA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gacaacttca aagcaaaatg aagttcgttc tgctgctttc cctcattggg ttctgctggg
       61 ctcaatatga cccacatact tcagatggga ggactgctat tgtccacctg ttcgagtggc
      121 gctgggttga tattgccaag gaatgtgagc gatacttagc tcctaaggga tttggagggg
      181 tgcaggtctc tccacccaat gaaaacgttg tagttcataa cccatcaaga ccttggtggg
      241 aaagatacca accaatcagc tataaaatct gcacaaggtc tggaaatgaa gatgaattca
      301 gagacatggt gacaaggtgc aacaatgttg gtgtccgtat ttatgtggat gctgtcatta
      361 accacatgtg tggcgcaggc aatcctgcag gaacaagcag tacctgtgga agttacctca
      421 atccaaataa cagggaattc ccagcagttc catactctgc ttgggacttt aacgataata
      481 aatgtaatgg agaaattgat aactacaatg atgcttatca ggtcagaaat tgtcgtctga
      541 ctggccttct ggatcttgca cttgagaaag attatgttcg taccaaggtg gctgactata
      601 tgaaccatct cattgacatt ggagtagcag ggttcagact tgatgctgct aagcacatgt
      661 ggcctggaga cataaaggca gttttggaca aactgcataa tctcaataca aaatggttct
      721 cccaaggaag cagacctttc attttccaag aggtcattga tctgggtggt gaggcaatta
      781 aaggtagtga gtactttgga aatggccgtg tgacagaatt caagtatggt gcaaaacttg
      841 gcacagttat ccgcaagtgg aatggcgaga agatgtccta tttaaagaac tggggagaag
      901 gttggggtat ggtgccttct gacagagccc ttgtgtttgt ggacaaccat gataatcagc
      961 gaggacatgg tgctggagga tcatccatcc tgacattctg ggatgctaga atgtataaaa
     1021 tggctgttgg atttatgttg gctcatcctt atggattcac aagagtaatg tcaagttacc
     1081 gttggaatag aaatttccag aatggaaaag atcagaatga ctggattgga ccacccaata
     1141 acaatggagt aacaaaagaa gtgaccatta atgcagacac tacttgtggc aatgactggg
     1201 tctgtgaaca cagatggcgt caaatcagga acatggttgc cttcagaaat gtggtcaatg
     1261 gtcagccttt ttcaaactgg tgggataata acagcaacca agtagctttt agcagaggaa
     1321 acagaggatt cattgtcttt aacaatgatg actgggcttt gtcagccact ttacagactg
     1381 gtcttcctgc tggcacatac tgtgatgtca tctctggaga taaggtcgat ggcaattgca
     1441 ctggacttag agtgaatgtt ggcagtgatg gcaaagctca cttttccatt agtaactctg
     1501 ctgaggaccc atttattgca atccatgctg actcaaaatt gtaagaatct atattaaaga
     1561 gatttggatt aagcatcaat tgcatgtgtt tctttttcat atgctctgta ctatattttt
     1621 atttctattt tgtttcttta tttttatgtt tccatgtgtg ctttgtcttc tgagccctgg
     1681 agtgtatgta ggatgtttga acaaccctgt gctgtggcat aacctctcta cattttccca
     1741 tgagctgtgc tgatcaaact aaatccatga ggcttccctg gaactcactt ttatctgcta
     1801 ttctatgttg ttatctcata tgccctttaa ttcttgcagt ttatttcctt gtagtaaaaa
     1861 ccatagacaa ctgtcttaat tagggtttct attcctgcac aaacatcatg accaagaagc
     1921 aagttgggaa ggaaagggtt tattcagctt aaacttctat actgctgttc atcaccaagg
     1981 aagtcaggac tggaactcaa gcaggtcagg aagcaggagc tgatgcaagg ccatggagtg
     2041 atgttcttta ctggcttgtc tcctctggct tgctcagcct gttctcttat agaaccaaga
     2101 ctatcagctc agagatggca ccacccacaa aggaacctcc ctgcttgatc actaattgag
     2161 aaaatgcatt gcagctggat ctcatggagg catttcccca attgaatctc ttttctctga
     2221 gataactcca gcctgtgtca agttgacaca aaactagcca gtacaacaat aaacttgaat
     2281 aggcaaatat cttaaaactc acataaaatc atctgataac aacatcctaa atatcctaag
     2341 cattagcaga atgtctaaat gtttttttct aataaattct caaatatctt aatagtgttc
     2401 tggcaaatat tttgctttac tattactata aattctttca tgtagccgtt ttaaagtatg
     2461 aaaatttatt ttcaataaat ttcattgaca atctaattac tcattaaaca tgcttttggc
     2521 tacttggaaa atccatcatt gaataatttc ttctcatgac tgttttcttc aagttccttt
     2581 cataattaat ttttccagga aagaagtttc ctcattccac aatacccacc tgtttctttt
     2641 ttggtgttct gttatgcatg gcctgtggtt cttgtgatcg gggagaccct tgttcagtag
     2701 aatgtctctg ctggcgattt ggcggcttgt gtgaccgctg atgtttaggg tactggggtt
     2761 ttaggaactg gcataacctt ggattctgga taagtcattg tctctggtag agcaaagagc
     2821 ttctgccaga gttgtggtat gggacatgga gatgagtgaa ggggccactg ttggggaaag
     2881 ttccacaggc tctgaggaaa tgttggtggt ctgcctcagg gcctgacctg atagagcagg
     2941 gtgattctgc tggagctatg ggtcaagata tggagacctg gaaggcatgc acttgtacct
     3001 gctgtgctat gttgctggct catctgtact ttgattttta aagtttattt attttttacc
     3061 aatagcctta aacaatgaac ttgaataggt agacatgatg aattgcacat gtaatcatta
     3121 gataacttca tcccaaattt actatgcatt aggagaaaat ctaaattttt tttcgaagaa
     3181 aataatcaga actttggatg gcttcccaag aaatatcttt ttaccattaa tatgatttcc
     3241 ttcatgttcc cctatatatt ttatgaaaaa gtgtttcaat ataaattcac tcacttatta
     3301 aacatgcttt ggttacttta aatctcatca ttgctcaatt tcctctggtg gccatttttt
     3361 aaaaaaattt gtcttataaa tagttatttc aagaaaaaaa atatcttttt ttaattccac
     3421 aatactaaca atactgacag cacatattgt aacctatctt gttactcagt taatggcatt
     3481 ctttgtttgg tgtacagaag cattttagtg tcatgaattc ctgttcttat tgtatgagaa
     3541 attgcaatca cattcagtaa agccttcctt ttgcaaatga gtttaa
//
