LOCUS       NM_001013814            2047 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus aminomethyltransferase (Amt), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001013814 XM_486257
VERSION     NM_001013814.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Peng,M.Z., Shao,Y.X., Li,X.Z., Zhang,K.D., Cai,Y.N., Lin,Y.T.,
            Jiang,M.Y., Liu,Z.C., Su,X.Y., Zhang,W., Jiang,X.L. and Liu,L.
  TITLE     Mitochondrial FAD shortage in SLC25A32 deficiency affects
            folate-mediated one-carbon metabolism
  JOURNAL   Cell Mol Life Sci 79 (7), 375 (2022)
   PUBMED   35727412
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Koutelou,E., Wang,L., Schibler,A.C., Chao,H.P., Kuang,X., Lin,K.,
            Lu,Y., Shen,J., Jeter,C.R., Salinger,A., Wilson,M., Chen,Y.C.,
            Atanassov,B.S., Tang,D.G. and Dent,S.Y.R.
  TITLE     USP22 controls multiple signaling pathways that are essential for
            vasculature formation in the mouse placenta
  JOURNAL   Development 146 (4) (2019)
   PUBMED   30718289
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2047)
  AUTHORS   Rhee,C., Edwards,M., Dang,C., Harris,J., Brown,M., Kim,J. and
            Tucker,H.O.
  TITLE     ARID3A is required for mammalian placenta development
  JOURNAL   Dev Biol 422 (2), 83-91 (2017)
   PUBMED   27965054
REFERENCE   4  (bases 1 to 2047)
  AUTHORS   Vessey,J.P., Amadei,G., Burns,S.E., Kiebler,M.A., Kaplan,D.R. and
            Miller,F.D.
  TITLE     An asymmetrically localized Staufen2-dependent RNA complex
            regulates maintenance of mammalian neural stem cells
  JOURNAL   Cell Stem Cell 11 (4), 517-528 (2012)
   PUBMED   22902294
REFERENCE   5  (bases 1 to 2047)
  AUTHORS   Narisawa,A., Komatsuzaki,S., Kikuchi,A., Niihori,T., Aoki,Y.,
            Fujiwara,K., Tanemura,M., Hata,A., Suzuki,Y., Relton,C.L.,
            Grinham,J., Leung,K.Y., Partridge,D., Robinson,A., Stone,V.,
            Gustavsson,P., Stanier,P., Copp,A.J., Greene,N.D., Tominaga,T.,
            Matsubara,Y. and Kure,S.
  TITLE     Mutations in genes encoding the glycine cleavage system predispose
            to neural tube defects in mice and humans
  JOURNAL   Hum Mol Genet 21 (7), 1496-1503 (2012)
   PUBMED   22171071
REFERENCE   6  (bases 1 to 2047)
  AUTHORS   Backofen,B., Jacob,R., Serth,K., Gossler,A., Naim,H.Y. and Leeb,T.
  TITLE     Cloning and characterization of the mammalian-specific nicolin 1
            gene (NICN1) encoding a nuclear 24 kDa protein
  JOURNAL   Eur J Biochem 269 (21), 5240-5245 (2002)
   PUBMED   12392556
REFERENCE   7  (bases 1 to 2047)
  AUTHORS   Backofen,B. and Leeb,T.
  TITLE     Genomic organization of the murine aminomethyltransferase gene
            (Amt)
  JOURNAL   DNA Seq 13 (4), 179-183 (2002)
   PUBMED   12487019
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161260.2.
            
            On Jun 22, 2018 this sequence version replaced NM_001013814.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC132277.1, SRR5189670.96843.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AC161260.2         172992-173112       c
            122-289             AC161260.2         172722-172889       c
            290-370             AC161260.2         172225-172305       c
            371-502             AC161260.2         171118-171249       c
            503-581             AC161260.2         170559-170637       c
            582-727             AC161260.2         170127-170272       c
            728-908             AC161260.2         169303-169483       c
            909-1064            AC161260.2         168789-168944       c
            1065-2047           AC161260.2         167715-168697       c
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.19 cM"
     gene            1..2047
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /note="aminomethyltransferase"
                     /db_xref="GeneID:434437"
                     /db_xref="MGI:MGI:3646700"
     exon            1..121
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1243
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /EC_number="2.1.2.10"
                     /note="aminomethyltransferase, mitochondrial; glycine
                     cleavage system T protein"
                     /codon_start=1
                     /product="aminomethyltransferase, mitochondrial precursor"
                     /protein_id="NP_001013836.1"
                     /db_xref="CCDS:CCDS23519.1"
                     /db_xref="GeneID:434437"
                     /db_xref="MGI:MGI:3646700"
                     /translation="MHRIVSVVAPLGFRLQAQPLVQSRPLSSVQDVLRRTPLYDFHLA
                     HGGKMVAFAGWSLPVQYRDSHVDSHLHTRRHCSLFDVSHMLQTKIFGCDRVKLLESVV
                     VGDIAELRPNQGTLSLFTNEAGGILDDLIVSNTSEGHLYVVSNAGCRDKDLALMQDKV
                     KEFQNRGLDVGLEVVENALLALQGPTATQVLQAGVTDDMKKLPFMTSAVMEVFGVSGC
                     RVTRCGYTGEDGVEISVPATGAVHLATTLLKNPEVKLAGLAARDSLRLEAGLCLYGND
                     IDEHTTPVEGSLSWTLGKRRRIAMDFPGAKIIVPQLKGEVQRRRVGLICEGAPVRAHS
                     PILNTEGTVIGTVTSGCPSPSLKKNVAMGYVPFKYSRPGTQLLVEVRRKQQMTVVSKM
                     PFVPTNYYTLK"
     exon            122..289
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            290..370
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            371..502
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            503..581
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            582..727
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            728..908
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1064
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..2047
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2021..2026
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /note="hexamer: AATAAA"
     polyA_site      2047
                     /gene="Amt"
                     /gene_synonym="EG434437; GCVT"
                     /note="major polyA site"
ORIGIN      
        1 agtgtgccgg ccgcaccagt tgcgggtgac aatgcatcgg atagtcagtg tggtagcccc
       61 tctgggtttt cgcctgcagg cacaaccctt ggtccagagt cgcccactca gttctgtaca
      121 ggatgtgctc cgcaggactc cactctatga cttccaccta gctcatggag gaaaaatggt
      181 ggcgtttgca gggtggagtc tgcctgtaca ataccgtgac agtcatgttg attcacacct
      241 gcacacacgc cggcattgct ctctctttga tgtgtcccac atgctacaga ccaagatatt
      301 tggctgtgac cgagtgaagc tgctggagag tgtagtggtt ggagacattg cggaactaag
      361 gcctaaccag ggaacactct ctttgtttac caacgaagct ggaggcatct tagacgactt
      421 gattgtaagc aatacttctg aggggcacct gtatgtagta tccaatgctg gctgccggga
      481 caaggacttg gctctcatgc aggacaaggt caaggagttc cagaacaggg gcctggatgt
      541 aggcctggag gtggtagaga atgccctgtt agctctgcaa ggacccacgg caacacaggt
      601 gttacaggct ggggtgacag atgatatgaa gaaactaccc ttcatgacaa gtgctgtgat
      661 ggaggtgttc ggtgtgtctg gctgtcgtgt gacccgctgt ggctacacag gagaagatgg
      721 tgttgagata tcagtcccag caacaggggc agtccacttg gcaactactc tgttgaaaaa
      781 tccagaggta aagctggcag gactagcagc ccgagatagc ctgcgcctgg aggcaggcct
      841 ctgtctgtat gggaatgaca tcgatgaaca tactacacct gtggagggca gcctcagctg
      901 gacactgggg aagcgccgcc gcattgctat ggacttccca ggagccaaaa tcattgttcc
      961 ccaactgaag ggtgaggtac agcggagacg tgtggggctg atatgtgaag gggctccagt
     1021 gagagcacac agtcctattt tgaacacaga aggcactgtg ataggtactg tgaccagtgg
     1081 ctgcccttca cccagcctga agaaaaatgt ggcgatgggt tatgtgccat ttaagtacag
     1141 tcgaccaggg acacagctgc tggtggaagt ccggcggaag cagcaaatga cagtggttag
     1201 caaaatgccc tttgtgccca ccaactatta tactctcaag tgaagatggc ccatggggaa
     1261 gcccttccct ccaggagtgt ttctctagag ggcatggcat agtgcttagt cagaacagtg
     1321 gagtacaact cccacgggtg gaagctgaat gaggtttgct gggtctgctt agggttgggg
     1381 agagaatcct atctagaatc tttcccttac aagccattcc aattttaggg cccaattttt
     1441 tttttttttt tttttttttt tttttggttt ttcgagacag ggtttctctg tatatccctg
     1501 gctgtcctga aactcacttt gtagaccagg ctggcctcca actcagaaat ctgcctgcct
     1561 ctgcctctga gtgctgggat taaaggcgta gggcccaatt tttaaatgac aaatcacctt
     1621 acttaatgtc ctgttttagc ccacagtccc actaacctta atctgcctgg tgctgacttt
     1681 ggaacaaagg tcatcctgcc tttgtgtgga gggtaacacc tccgcccacc tcactatggc
     1741 ttctctaatt tcaaagaact attactgtgg gcagtatgga cttaggtacc ttctccactt
     1801 tgcatactgc tgcctttcct tagatagaaa tgactcctga cgtggtgttg gctgagttgt
     1861 tcctgtgtgc aaacatagag atggctaagc aaacatggga ctcacccttc cacattctct
     1921 agtcaaccca gcctgctgtg cagcaagaca tggctggttt aatacttggg ctgacctcgg
     1981 tcagttgttg tgaataatta tactttacta actcttgaaa aataaaatta atttctttct
     2041 tacttgc
//
