LOCUS       NM_033603               1700 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus amnionless (Amn), mRNA.
ACCESSION   NM_033603 XM_030247005
VERSION     NM_033603.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Liang,S., Wang,Y., Kang,M., Deng,J., Chen,L., Hong,X., Hou,F.F. and
            Zhang,F.
  TITLE     Generation and characterization of an inducible renal proximal
            tubule-specific CreERT2 mouse
  JOURNAL   Front Cell Dev Biol 11, 1171637 (2023)
   PUBMED   37215091
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Perea-Gomez,A., Cases,O., Lelievre,V., Pulina,M.V., Collignon,J.,
            Hadjantonakis,A.K. and Kozyraki,R.
  TITLE     Loss of Cubilin, the intrinsic factor-vitamin B12 receptor, impairs
            visceral endoderm endocytosis and endodermal patterning in the
            mouse
  JOURNAL   Sci Rep 9 (1), 10168 (2019)
   PUBMED   31308417
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1700)
  AUTHORS   Kulinski,T.M., Casari,M.R., Guenzl,P.M., Wenzel,D., Andergassen,D.,
            Hladik,A., Datlinger,P., Farlik,M., Theussl,H.C., Penninger,J.M.,
            Knapp,S., Bock,C., Barlow,D.P. and Hudson,Q.J.
  TITLE     Imprinted expression in cystic embryoid bodies shows an embryonic
            and not an extra-embryonic pattern
  JOURNAL   Dev Biol 402 (2), 291-305 (2015)
   PUBMED   25912690
REFERENCE   4  (bases 1 to 1700)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   5  (bases 1 to 1700)
  AUTHORS   Zheng,H.J., Tsukahara,M., Liu,E., Ye,L., Xiong,H., Noguchi,S.,
            Suzuki,K. and Ji,Z.S.
  TITLE     The novel helicase helG (DHX30) is expressed during gastrulation in
            mice and has a structure similar to a human DExH box helicase
  JOURNAL   Stem Cells Dev 24 (3), 372-383 (2015)
   PUBMED   25219788
REFERENCE   6  (bases 1 to 1700)
  AUTHORS   Witherden,A.S., Hafezparast,M., Nicholson,S.J., Ahmad-Annuar,A.,
            Bermingham,N., Arac,D., Rankin,J., Iravani,M., Ball,S., Peters,J.,
            Martin,J.E., Huntley,D., Hummerich,H., Sergot,M. and Fisher,E.M.
  TITLE     An integrated genetic, radiation hybrid, physical and transcription
            map of a region of distal mouse chromosome 12, including an
            imprinted locus and the 'Legs at odd angles' (Loa) mutation
  JOURNAL   Gene 283 (1-2), 71-82 (2002)
   PUBMED   11867214
REFERENCE   7  (bases 1 to 1700)
  AUTHORS   Kalantry,S., Manning,S., Haub,O., Tomihara-Newberger,C., Lee,H.G.,
            Fangman,J., Disteche,C.M., Manova,K. and Lacy,E.
  TITLE     The amnionless gene, essential for mouse gastrulation, encodes a
            visceral-endoderm-specific protein with an extracellular
            cysteine-rich domain
  JOURNAL   Nat Genet 27 (4), 412-416 (2001)
   PUBMED   11279523
REFERENCE   8  (bases 1 to 1700)
  AUTHORS   Tomihara-Newberger,C., Haub,O., Lee,H.G., Soares,V., Manova,K. and
            Lacy,E.
  TITLE     The amn gene product is required in extraembryonic tissues for the
            generation of middle primitive streak derivatives
  JOURNAL   Dev Biol 204 (1), 34-54 (1998)
   PUBMED   9851841
REFERENCE   9  (bases 1 to 1700)
  AUTHORS   Manova,K., Tomihara-Newberger,C., Wang,S., Godelman,A.,
            Kalantry,S., Witty-Blease,K., De Leon,V., Chen,W.S., Lacy,E. and
            Bachvarova,R.F.
  TITLE     Apoptosis in mouse embryos: elevated levels in pregastrulae and in
            the distal anterior region of gastrulae of normal and mutant mice
  JOURNAL   Dev Dyn 213 (3), 293-308 (1998)
   PUBMED   9825865
REFERENCE   10 (bases 1 to 1700)
  AUTHORS   Wang,X., Bornslaeger,E.A., Haub,O., Tomihara-Newberger,C.,
            Lonberg,N., Dinulos,M.B., Disteche,C.M., Copeland,N., Gilbert,D.J.,
            Jenkins,N.A. and Lacy,E.
  TITLE     A candidate gene for the amnionless gastrulation stage mouse
            mutation encodes a TRAF-related protein
  JOURNAL   Dev Biol 177 (1), 274-290 (1996)
   PUBMED   8660894
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB873437.1, BC087954.1 and
            AC127580.3.
            
            On or before Jun 26, 2023 this sequence version replaced
            XM_030247005.2, NM_033603.2.
            
            Summary: This gene encodes a type I transmembrane protein. The
            encoded protein is an essential component of the cubulin receptor
            complex which is thought to play a role in coordinating growth and
            patterning of the embryo. This protein is thought to modulate a
            bone morphogenetic protein (BMP) signaling pathway. A homoygous
            mutation in the mouse gene results in the lack of an amnion in
            embryos. Mutations in the human gene are associated with
            Megaloblastic Anemia-1. [provided by RefSeq, Sep 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF320619.1, BC087954.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BB873437.1         2-3
            3-1528              BC087954.1         1-1526
            1529-1700           AC127580.3         112289-112460       c
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 60.94 cM"
     gene            1..1700
                     /gene="Amn"
                     /note="amnionless"
                     /db_xref="GeneID:93835"
                     /db_xref="MGI:MGI:1934943"
     exon            1..69
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1403
                     /gene="Amn"
                     /note="protein amnionless; type I transmembrane protein"
                     /codon_start=1
                     /product="protein amnionless precursor"
                     /protein_id="NP_291081.2"
                     /db_xref="CCDS:CCDS36564.1"
                     /db_xref="GeneID:93835"
                     /db_xref="MGI:MGI:1934943"
                     /translation="MGALGRVLLWLQLCAMTRAAYKLWVPNTSFDTASNWNQNRTPCA
                     GDAVQFPADKMVSVLVRDSHAISDMLLPLDGELVLASGAALSAAGGDSDPACNPGAPL
                     LFRNPDRFSWLDPHLWSSGTQAPGLFSVDAERVPCSYDDVLFPRDGSFRVALGPGPNP
                     VHVRSVSAVGQTFSRDEDLTAFLASREGRLRFHGSGALRVGSQACTDASGCVCGNAEM
                     LPWICASLLQPLGGRCPQAACQDPLLPQGQCCDLCGAIVSLTHDPTFDLERYRARLLD
                     LFLKQPQYQGLQVAVSKVLRDAHTEIQVVLVETEHATGAAGQLGHALLQDAVAQGSVL
                     GIVSATLRQSGKPMTADSELNQSSSGAGLAGGVAALVLLALLGTVLLLLHRSGRLRWR
                     RHEDAEPVSAGLPLGFRNPIFDAIVFKQQPSVELPDSAQKVDILDIDTKFGCFVNPLF
                     AGEAEAEA"
     exon            70..188
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            189..233
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            234..321
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            322..539
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            540..677
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            678..786
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            787..869
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1017
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1195
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1289
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1700
                     /gene="Amn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1501..1506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amn"
                     /note="hexamer: AATAAA"
     polyA_site      1533
                     /gene="Amn"
                     /note="major polyA site"
     regulatory      1679..1684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Amn"
                     /note="hexamer: ATTAAA"
     polyA_site      1698
                     /gene="Amn"
ORIGIN      
        1 gcatctgtga gagcctcggc ggagagatgg gtgctctggg ccgggtcctg ctgtggctgc
       61 agctctgtgc aatgactcgg gccgcctaca aactttgggt ccccaatacc tccttcgata
      121 ctgcctccaa ctggaaccag aaccggaccc cgtgcgcggg agatgcggtt cagttcccgg
      181 cggacaagat ggtgtcggtc ctggtacgag acagtcacgc catctcggat atgctcctgc
      241 ctctggacgg ggagttggtc ctggcctcgg gagctgcact cagcgctgca ggaggcgact
      301 cggacccagc ttgtaaccct ggcgccccgt tgctcttccg caatcccgat cgcttctcct
      361 ggcttgaccc gcacctctgg agctccggga ctcaggcgcc cgggctcttc tccgtggacg
      421 ccgagcgcgt gccctgcagc tacgacgacg tcctcttccc gcgcgatgga tctttccgcg
      481 tggcgcttgg cccaggcccc aacccggtgc acgttcgcag cgtctcggca gtgggccaga
      541 cgttctcgcg cgacgaggac ctgactgctt tcctggcgtc ccgcgagggc cgcctgcgct
      601 tccacgggtc gggcgcgctg agagtggggt cccaagcttg caccgacgcg tcgggctgcg
      661 tctgtggcaa cgccgagatg ctgccgtgga tctgcgcgtc tctgctgcag cccttgggcg
      721 gccgctgtcc ccaggccgcc tgccaggacc ccctactgcc tcaaggacaa tgctgtgacc
      781 tctgcggagc tatcgtgtcg ttgacccacg accccacatt tgacctggag cggtaccgag
      841 cgcggctgct ggacctcttc ctgaagcagc cccagtacca gggcctgcag gtggctgtgt
      901 ccaaggtgct gcgcgacgcc cacaccgaga tccaggtggt gctggtggag accgagcacg
      961 cgaccggcgc ggcggggcag ctgggccacg cgctcctcca ggacgcggtg gcacaaggca
     1021 gcgtgctcgg tatcgtgtcg gcgaccctgc ggcagtccgg caagcctatg acggcagact
     1081 cagagctgaa tcagagctcg tccggtgccg ggctggcggg cggcgtggcg gccttggtgc
     1141 tgctggctct gctggggacc gtgctccttc tgctgcaccg cagcggaagg ctcaggtgga
     1201 gaaggcatga agacgctgag cccgtatcgg ctgggctgcc gctgggtttc cgaaatccga
     1261 ttttcgacgc gattgtcttc aagcagcagc cttcggtcga gctacctgac tcagctcaga
     1321 aggtggacat cttggacatt gataccaagt tcggctgctt cgttaaccca ctctttgctg
     1381 gggaggcaga ggcagaggcc tgacctgcta gaagggtact aggcttctaa cctgtggact
     1441 gttcaccccc tcggagaccg tgacttctct cctggtggca aggacagact taccttgccc
     1501 aataaagagt tgcttgtttg ttttttaact tgatagcctg gtcctacttg taaccttgct
     1561 ttacttgagc gatggagatc tgaactctgg tcctcagacc cacgccgcaa gtgctgttac
     1621 cattcgggcg cagtgttttg ttttgaggtg agcgtctcac catgtactct aggaaggcat
     1681 taaacttttg ggctcagata
//
